(Circular) (Six-base) MAPSORT of: pFN21AB5835.seq Check: 5026 from: 1 to: 6295 pFN21AB5835 6295 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4105 278 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3638 1937 Size: 1701 4594 Acc65I G'GTAC_C Cuts at: 3616 3616 Size: 6295 AclI AA'CG_TT Cuts at: 1563 4422 4795 1563 Size: 2859 373 3063 Fragments arranged by size: 3063 2859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2615 3549 4358 5742 6010 1632 Size: 983 934 809 1384 268 1917 Fragments arranged by size: 1917 1384 983 934 809 268 AflII C'TTAA_G Cuts at: 820 1017 3272 820 Size: 197 2255 3843 Fragments arranged by size: 3843 2255 197 AflIII A'CryG_T Cuts at: 1203 2389 5194 6171 1203 Size: 1186 2805 977 1327 Fragments arranged by size: 2805 1327 1186 977 AgeI A'CCGG_T Cuts at: 5126 5126 Size: 6295 AhdI GACnn_n'nnGTC Cuts at: 2569 5024 2569 Size: 2455 3840 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6295 AlwNI CAG_nnn'CTG Cuts at: 4038 5610 4038 Size: 1572 4723 ApaI G_GGCC'C Cuts at: 2037 2177 3122 2037 Size: 140 945 5210 Fragments arranged by size: 5210 945 140 ApaLI G'TGCA_C Cuts at: 2084 4352 5508 2084 Size: 2268 1156 2871 Fragments arranged by size: 2871 2268 1156 ApoI r'AATT_y Cuts at: 1493 3138 3604 3830 3982 6091 1493 Size: 1645 466 226 152 2109 1697 Fragments arranged by size: 2109 1697 1645 466 226 152 AseI AT'TA_AT Cuts at: 160 1032 4849 160 Size: 872 3817 1606 Fragments arranged by size: 3817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 3465 3620 1870 Size: 73 1522 155 4545 Fragments arranged by size: 4545 1522 155 73 BamHI G'GATC_C Cuts at: 3580 3625 3992 3580 Size: 45 367 5883 Fragments arranged by size: 5883 367 45 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3616 5071 618 Size: 325 217 627 1829 1455 1842 Fragments arranged by size: 1842 1829 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2037 2177 2364 2958 3122 3315 3418 3466 Size: 646 140 187 594 164 193 103 48 Cuts at: 3466 3614 1391 Size: 148 4072 Fragments arranged by size: 4072 646 594 193 187 164 148 140 103 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5324 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2790 2824 4486 4520 1903 Size: 34 50 34 769 34 1662 34 3678 Fragments arranged by size: 3678 1662 769 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2578 4187 5397 2578 Size: 1609 1210 3476 Fragments arranged by size: 3476 1609 1210 BclI T'GATC_A Cuts at: 1548 2931 3162 1548 Size: 1383 231 4681 Fragments arranged by size: 4681 1383 231 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 6295 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3385 4906 136 Size: 107 122 71 631 605 1713 1521 1525 Fragments arranged by size: 1713 1525 1521 631 605 122 107 71 BglII A'GATC_T Cuts at: 6290 6290 Size: 6295 BlpI GC'TnA_GC Cuts at: 3712 3712 Size: 6295 Bme1580I G_kGCm'C Cuts at: 1790 2037 2088 2177 3122 4356 5512 1790 Size: 247 51 89 945 1234 1156 2573 Fragments arranged by size: 2573 1234 1156 945 247 89 51 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6295 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2893 3031 3412 Size: 416 53 83 399 24 1476 138 381 Cuts at: 3412 3450 4026 4984 442 Size: 38 576 958 1753 Fragments arranged by size: 1753 1476 958 576 416 399 381 138 83 53 38 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3432 3432 Size: 6295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2348 2447 3053 3323 3329 4955 1239 Size: 1109 99 606 270 6 1626 2579 Fragments arranged by size: 2579 1626 1109 606 270 99 6 Bpu10I CC'TnA_GC Cuts at: 3359 3359 Size: 6295 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2866 3571 4420 5285 5583 2866 Size: 705 849 865 298 3578 Fragments arranged by size: 3578 865 849 705 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4958 882 Size: 617 3459 2219 Fragments arranged by size: 3459 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2560 4086 493 Size: 711 1356 1526 2702 Fragments arranged by size: 2702 1526 1356 711 BsaBI GATnn'nnATC Cuts at: 6289 6289 Size: 6295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4102 4484 5119 275 Size: 53 83 186 936 2569 382 635 1451 Fragments arranged by size: 2569 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4728 5126 5400 5547 5958 4728 Size: 398 274 147 411 5065 Fragments arranged by size: 5065 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2471 2501 1178 Size: 30 165 30 1068 30 4972 Fragments arranged by size: 4972 1068 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6295 BseYI C'CCAG_C Cuts at: 2177 3227 5498 5994 2177 Size: 1050 2271 496 2478 Fragments arranged by size: 2478 2271 1050 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2101 1163 Size: 573 365 5357 Fragments arranged by size: 5357 573 365 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3745 4506 5534 1540 Size: 275 186 1744 761 1028 2301 Fragments arranged by size: 2301 1744 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2088 2559 3418 3466 4356 4441 5512 2088 Size: 471 859 48 890 85 1071 2871 Fragments arranged by size: 2871 1071 890 859 471 85 48 BsmI GAATG_Cn' Cuts at: 3806 3899 3806 Size: 93 6202 BsmBI CGTCTCn'nnnn_ Cuts at: 2577 2577 Size: 6295 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2088 2177 2364 2559 2958 3122 Size: 399 247 51 89 187 195 399 164 Cuts at: 3122 3315 3418 3466 3614 4356 4441 5512 1391 Size: 193 103 48 148 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 399 247 195 193 187 164 148 103 89 85 51 48 BspEI T'CCGG_A Cuts at: 5958 5958 Size: 6295 BspHI T'CATG_A Cuts at: 4184 5914 4184 Size: 1730 4565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2285 3650 844 Size: 1441 1365 3489 Fragments arranged by size: 3489 1441 1365 BsrBI CCG'CTC Cuts at: 1394 1823 4182 1394 Size: 429 2359 3507 Fragments arranged by size: 3507 2359 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2088 2599 4790 4964 65 Size: 1183 840 511 2191 174 1396 Fragments arranged by size: 2191 1396 1183 840 511 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4939 5126 1700 Size: 110 3129 187 2869 Fragments arranged by size: 3129 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6295 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6295 BssSI C'ACGA_G Cuts at: 4355 5367 4355 Size: 1012 5283 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6295 BstBI TT'CG_AA Cuts at: 5099 5099 Size: 6295 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6295 BstYI r'GATC_y Cuts at: 1972 3580 3625 3992 4377 4394 5846 5932 5944 Size: 1608 45 367 385 17 1452 86 12 Cuts at: 5944 6290 1972 Size: 346 1977 Fragments arranged by size: 1977 1608 1452 385 367 346 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 3249 3381 3249 Size: 132 6163 BtgI C'CryG_G Cuts at: 513 1065 2069 513 Size: 552 1004 4739 Fragments arranged by size: 4739 1004 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5556 Fragments arranged by size: 5556 683 56 BtsI GCAGTG_nn' Cuts at: 3185 3811 4605 4625 3185 Size: 626 794 20 4855 Fragments arranged by size: 4855 794 626 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6295 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6260 DraI TTT'AAA Cuts at: 2943 3599 2943 Size: 656 5639 DraIII CAC_nnn'GTG Cuts at: 2330 2330 Size: 6295 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5302 809 Size: 507 3986 1802 Fragments arranged by size: 3986 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3204 3742 4631 Size: 54 1425 50 275 18 1374 538 889 Cuts at: 4631 6260 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1374 889 538 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3742 1537 Size: 275 1930 4090 Fragments arranged by size: 4090 1930 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4225 1353 Size: 2872 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4882 5256 5402 387 Size: 1064 138 3293 374 146 1280 Fragments arranged by size: 3293 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3416 3464 3416 Size: 48 6247 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2348 2447 2615 3053 3323 3329 3549 Size: 393 716 99 168 438 270 6 220 Cuts at: 3549 4358 4955 5742 6010 1239 Size: 809 597 787 268 1524 Fragments arranged by size: 1524 809 787 716 597 438 393 270 268 220 168 99 6 EcoNI CCTnn'n_nnAGG Cuts at: 2129 3501 2129 Size: 1372 4923 EcoO109I rG'GnC_Cy Cuts at: 2033 2034 2174 3253 2033 Size: 1 140 1079 5075 Fragments arranged by size: 5075 1079 140 1 EcoRI G'AATT_C Cuts at: 3138 3604 3982 3138 Size: 466 378 5451 Fragments arranged by size: 5451 466 378 EcoRV GAT'ATC Cuts at: 2650 2650 Size: 6295 FspI TGC'GCA Cuts at: 4801 4801 Size: 6295 HaeII r_GCGC'y Cuts at: 1678 5442 1678 Size: 3764 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2555 2587 2859 2891 3412 Size: 32 135 32 983 32 272 32 521 Cuts at: 3412 3444 4936 4968 5010 5042 1373 Size: 32 1492 32 42 32 2626 Fragments arranged by size: 2626 1492 983 521 272 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3639 3885 669 Size: 988 281 1701 246 3079 Fragments arranged by size: 3079 1701 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6295 HpaI GTT'AAC Cuts at: 3885 3885 Size: 6295 KpnI G_GTAC'C Cuts at: 3620 3620 Size: 6295 MfeI C'AATT_G Cuts at: 3894 3894 Size: 6295 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2457 5409 5593 1305 Size: 1152 2952 184 2007 Fragments arranged by size: 2952 2007 1152 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3206 10 Size: 54 1425 343 1374 3099 Fragments arranged by size: 3099 1425 1374 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2691 3054 4253 4612 4771 518 Size: 648 1525 363 1199 359 159 2042 Fragments arranged by size: 2042 1525 1199 648 363 359 159 MspA1I CmG'CkG Cuts at: 1606 2834 3016 3711 4388 5536 5781 1606 Size: 1228 182 695 677 1148 245 2120 Fragments arranged by size: 2120 1228 1148 695 677 245 182 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5743 NdeI CA'TA_TG Cuts at: 387 2974 387 Size: 2587 3708 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 6295 NspI r_CATG'y Cuts at: 1132 1229 2188 2299 2393 3292 3335 3653 5198 Size: 97 959 111 94 899 43 318 1545 Cuts at: 5198 1132 Size: 2229 Fragments arranged by size: 2229 1545 959 899 318 111 97 94 43 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6295 PciI A'CATG_T Cuts at: 2389 5194 2389 Size: 2805 3490 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6295 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PmlI CAC'GTG Cuts at: 2560 2560 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2135 2167 4364 4396 5877 5909 2135 Size: 32 2197 32 1481 32 2521 Fragments arranged by size: 2521 2197 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3253 3253 Size: 6295 PshAI GACnn'nnGTC Cuts at: 1613 3497 1613 Size: 1884 4411 PsiI TTA'TAA Cuts at: 3865 3865 Size: 6295 PspOMI G'GGCC_C Cuts at: 2033 2173 3118 2033 Size: 140 945 5210 Fragments arranged by size: 5210 945 140 PspXI vC'TCGA_Gb Cuts at: 3465 3465 Size: 6295 PstI C_TGCA'G Cuts at: 830 2142 2165 3647 830 Size: 1312 23 1482 3478 Fragments arranged by size: 3478 1482 1312 23 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 PvuII CAG'CTG Cuts at: 2834 3016 2834 Size: 182 6113 SacI G_AGCT'C Cuts at: 3418 3466 3418 Size: 48 6247 SalI G'TCGA_C Cuts at: 1936 3637 1936 Size: 1701 4594 SbfI CC_TGCA'GG Cuts at: 3647 3647 Size: 6295 ScaI AGT'ACT Cuts at: 4543 6255 4543 Size: 1712 4583 SfcI C'TryA_G Cuts at: 826 1045 2138 2161 2637 3643 4778 5459 5650 Size: 219 1093 23 476 1006 1135 681 191 Cuts at: 5650 826 Size: 1471 Fragments arranged by size: 1471 1135 1093 1006 681 476 219 191 23 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 3622 1872 Size: 1750 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2845 3272 3465 3586 4399 5300 Size: 197 926 902 427 193 121 813 901 Cuts at: 5300 5562 820 Size: 262 1553 Fragments arranged by size: 1553 926 902 901 813 427 262 197 193 121 SnaBI TAC'GTA Cuts at: 493 4086 493 Size: 3593 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6295 SphI G_CATG'C Cuts at: 1132 3292 3653 1132 Size: 2160 361 3774 Fragments arranged by size: 3774 2160 361 SspI AAT'ATT Cuts at: 5 52 4219 5 Size: 47 4167 2081 Fragments arranged by size: 4167 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6295 StyI C'CwwG_G Cuts at: 513 1065 3102 3734 513 Size: 552 2037 632 3074 Fragments arranged by size: 3074 2037 632 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3411 4322 4339 4492 4677 5122 325 Size: 1255 1831 911 17 153 185 445 1498 Fragments arranged by size: 1831 1498 1255 911 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3348 4541 6253 Size: 274 80 33 51 1443 1371 1193 1712 Cuts at: 6253 96 Size: 138 Fragments arranged by size: 1712 1443 1371 1193 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2448 2480 6255 6287 2448 Size: 32 3775 32 2456 Fragments arranged by size: 3775 2456 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6295 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XhoI C'TCGA_G Cuts at: 1943 3465 1943 Size: 1522 4773 XmaI C'CCGG_G Cuts at: 1870 3620 1870 Size: 1750 4545 XmnI GAAnn'nnTTC Cuts at: 2147 4424 2147 Size: 2277 4018 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4103 276 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BstXI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI