Restriction map for
pFN21AB5832
##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BbrPI BbsI BbvCI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tru9I Tsp509I XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 157 CGCG CG^CG
AcyI 1164 GGCGCC G[GA]^CG[CT]C
Alw44I 198 GTGCAC G^TGCAC
AlwNI 560 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 198 GTGCAC G^TGCAC
AspI 445 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1014 TGGCCA TGG^CCA
BamHI 378 GGATCC G^GATCC
BbeI 1164 GGCGCC GGCGC^C
BcgI 995 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 992 CTAG C^TAG
BglI 29 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 169 TACGTG [CT]AC^GT[GA]
BsaHI 1164 GGCGCC G[GA]^CG[CT]C
BsaI 692 GAGACC GGTCTC(1/5)
BseRI 645 CTCCTC GAGGAG(10/8)
BsmBI 551 CGTCTC CGTCTC(1/5)
BsmI 733 GCATTC GAATGC(1/-1)
BspHI 1382 TCATGA T^CATGA
BsrBI 1027 CCGCTC CCGCTC(-3/-3)
BstEII 162 GGTCACC G^GT[ACGT]ACC
BstPI 162 GGTCACC G^GT[ACGT]ACC
BstUI 157 CGCG CG^CG
EaeI 1014 TGGCCA [CT]^GGCC[GA]
EcoNI 760 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 162 GGTCACC G^GT[ACGT]ACC
EcoRV 175 GATATC GAT^ATC
EcoT14I 765 CCAAGG C^C[AT][AT]GG
HaeII 1164 GGCGCC [GA]GCGC^[CT]
Hin1I 1164 GGCGCC G[GA]^CG[CT]C
KasI 1164 GGCGCC G^GCGCC
MaeI 992 CTAG C^TAG
MluNI 1014 TGGCCA TGG^CCA
MscI 1014 TGGCCA TGG^CCA
MvnI 157 CGCG CG^CG
NarI 1164 GGCGCC GG^CGCC
PaeR7I 576 CTCGAG C^TCGAG
PflFI 445 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1222 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 891 GACTC GAGTC(4/5)
RcaI 1382 TCATGA T^CATGA
SbfI 871 CCTGCAGG CCTGCA^GG
ScaI 664 AGTACT AGT^ACT
SfoI 1164 GGCGCC GGC^GCC
SmlI 576 CTCGAG C^T[CT][GA]AG
Sse8387I 871 CCTGCAGG CCTGCA^GG
StyI 765 CCAAGG C^C[AT][AT]GG
TfiI 370 GATTC G^A[AT]TC
Tth111I 445 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1222 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 576 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 364 GTAC GT^AC
665 GTAC
AspHI 198 GTGCAC G[AT]GC[AT]^C
624 GAGCAC
BsiHKAI 198 GTGCAC G[AT]GC[AT]^C
624 GAGCAC
BstAPI 200 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
733 GCATTCCCTGC
BstYI 378 GGATCC [GA]^GATC[CT]
1364 GGATCT
DsaI 182 CCGTGG C^C[GA][CT]GG
303 CCGTGG
HinfI 370 GATTC G^A[ACGT]TC
891 GACTC
MaeII 170 ACGT A^CGT
947 ACGT
MaeIII 163 GTCAC ^GT[ACGT]AC
1039 GTCAC
MflI 378 GGATCC [GA]^GATC[CT]
1364 GGATCT
MslI 165 CACCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
486 CACCCCTGTG
NlaIII 261 CATG CATG^
1383 CATG
PpuMI 963 GGGACCC [GA]G^G[AT]CC[CT]
1019 AGGTCCC
PvuII 836 CAGCTG CAG^CTG
1358 CAGCTG
RsaI 364 GTAC GT^AC
665 GTAC
SapI 809 GCTCTTC GCTCTTC(1/4)
1301 GCTCTTC
SfaNI 346 GATGC GCATC(5/9)
787 GATGC
TaqI 143 TCGA T^CGA
577 TCGA
Tsp45I 163 GTCAC ^GT[GC]AC
1039 GTCAC
TthHB8I 143 TCGA T^CGA
577 TCGA
XhoII 378 GGATCC [GA]^GATC[CT]
1364 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIV PstI Sau3AI Sau96I ScrFI
SfcI SmaI TseI TspRI XmaCI
XmaI