(Circular) (Six-base) MAPSORT of: pFN21AB5832.seq Check: 7865 from: 1 to: 6148 pFN21AB5832 6148 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2219 2978 2219 Size: 759 5389 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3491 1937 Size: 1554 4594 Acc65I G'GTAC_C Cuts at: 3469 3469 Size: 6148 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2307 4211 5595 5863 1632 Size: 675 1904 1384 268 1917 Fragments arranged by size: 1917 1904 1384 675 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 5047 6024 1203 Size: 3844 977 1327 Fragments arranged by size: 3844 1327 977 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 2174 2495 1166 Size: 1008 321 4819 Fragments arranged by size: 4819 1008 321 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6148 AlwNI CAG_nnn'CTG Cuts at: 2570 3891 5463 2570 Size: 1321 1572 3255 Fragments arranged by size: 3255 1572 1321 ApaI G_GGCC'C Cuts at: 2030 2036 2466 3180 2030 Size: 6 430 714 4998 Fragments arranged by size: 4998 714 430 6 ApaLI G'TGCA_C Cuts at: 2203 4205 5361 2203 Size: 2002 1156 2990 Fragments arranged by size: 2990 2002 1156 ApoI r'AATT_y Cuts at: 1493 3457 3683 3835 5944 1493 Size: 1964 226 152 2109 1697 Fragments arranged by size: 2109 1964 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 2029 2035 2581 2972 3473 1870 Size: 73 86 6 546 391 501 4545 Fragments arranged by size: 4545 546 501 391 86 73 6 BamHI G'GATC_C Cuts at: 2383 3478 3845 2383 Size: 1095 367 4686 Fragments arranged by size: 4686 1095 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 2722 2906 2920 2977 Size: 325 217 627 304 631 184 14 57 Cuts at: 2977 3169 3469 4924 618 Size: 192 300 1455 1842 Fragments arranged by size: 1842 1455 631 627 325 304 300 217 192 184 57 14 BanII G_rGCy'C Cuts at: 1391 2030 2036 2466 2507 3180 3467 1391 Size: 639 6 430 41 673 287 4072 Fragments arranged by size: 4072 673 639 430 287 41 6 BbeI G_GCGC'C Cuts at: 3173 3173 Size: 6148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5177 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2989 3023 4339 4373 1903 Size: 34 1052 34 1316 34 3678 Fragments arranged by size: 3678 1316 1052 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4040 5250 4040 Size: 1210 4938 BclI T'GATC_A Cuts at: 1548 1548 Size: 6148 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 4759 136 Size: 107 122 71 631 605 368 2719 1525 Fragments arranged by size: 2719 1525 631 605 368 122 107 71 BglII A'GATC_T Cuts at: 6143 6143 Size: 6148 BlpI GC'TnA_GC Cuts at: 3565 3565 Size: 6148 Bme1580I G_kGCm'C Cuts at: 1790 2030 2036 2207 2466 2982 3180 4209 5365 Size: 240 6 171 259 516 198 1029 1156 Cuts at: 5365 1790 Size: 2573 Fragments arranged by size: 2573 1156 1029 516 259 240 198 171 6 BmgBI CAC'GTC Cuts at: 1318 2953 1318 Size: 1635 4513 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3118 3243 3879 Size: 416 53 83 399 24 1701 125 636 Cuts at: 3879 4837 442 Size: 958 1753 Fragments arranged by size: 1753 1701 958 636 416 399 125 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2095 2095 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2393 2823 3020 4808 1239 Size: 1154 430 197 1788 2579 Fragments arranged by size: 2579 1788 1154 430 197 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4273 5138 5436 4273 Size: 865 298 4985 Fragments arranged by size: 4985 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2691 4811 882 Size: 617 1192 2120 2219 Fragments arranged by size: 2219 2120 1192 617 BsaAI yAC'GTr Cuts at: 493 1204 2176 3939 493 Size: 711 972 1763 2702 Fragments arranged by size: 2702 1763 972 711 BsaBI GATnn'nnATC Cuts at: 6142 6142 Size: 6148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3170 3955 4337 4972 Size: 53 83 186 936 1637 785 382 635 Cuts at: 4972 275 Size: 1451 Fragments arranged by size: 1637 1451 936 785 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4581 4979 5253 5400 5811 4581 Size: 398 274 147 411 4918 Fragments arranged by size: 4918 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2075 2105 1178 Size: 30 165 30 672 30 5221 Fragments arranged by size: 5221 672 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2641 1186 Size: 1455 4693 BseYI C'CCAG_C Cuts at: 2110 2157 2810 2865 3200 3256 3365 5351 5847 Size: 47 653 55 335 56 109 1986 496 Cuts at: 5847 2110 Size: 2411 Fragments arranged by size: 2411 1986 653 496 335 109 56 55 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2519 2535 2694 1163 Size: 573 783 16 159 4617 Fragments arranged by size: 4617 783 573 159 16 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3598 4359 5387 1540 Size: 275 186 1597 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2207 2633 4209 4294 5365 2207 Size: 426 1576 85 1071 2990 Fragments arranged by size: 2990 1576 1071 426 85 BsmI GAATG_Cn' Cuts at: 2738 3659 3752 2738 Size: 921 93 5134 Fragments arranged by size: 5134 921 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2562 2562 Size: 6148 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2036 2207 2466 2507 2633 2982 Size: 399 240 6 171 259 41 126 349 Cuts at: 2982 3180 3467 4209 4294 5365 1391 Size: 198 287 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 349 287 259 240 198 171 126 85 41 6 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 3387 4037 5767 3387 Size: 650 1730 3768 Fragments arranged by size: 3768 1730 650 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2219 2685 2978 3503 844 Size: 1375 466 293 525 3489 Fragments arranged by size: 3489 1375 525 466 293 BsrBI CCG'CTC Cuts at: 1394 1823 3034 4035 1394 Size: 429 1211 1001 3507 Fragments arranged by size: 3507 1211 1001 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4643 4817 65 Size: 1183 3395 174 1396 Fragments arranged by size: 3395 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4792 4979 1700 Size: 110 2982 187 2869 Fragments arranged by size: 2982 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 4208 5220 4208 Size: 1012 5136 BstAPI GCAn_nnn'nTGC Cuts at: 794 2211 2744 794 Size: 1417 533 4198 Fragments arranged by size: 4198 1417 533 BstBI TT'CG_AA Cuts at: 4952 4952 Size: 6148 BstEII G'GTnAC_C Cuts at: 1184 2167 1184 Size: 983 5165 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2917 3161 3166 3365 2011 Size: 906 244 5 199 4794 Fragments arranged by size: 4794 906 244 199 5 BstYI r'GATC_y Cuts at: 1972 2383 3369 3478 3845 4230 4247 5699 5785 Size: 411 986 109 367 385 17 1452 86 Cuts at: 5785 5797 6143 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 986 411 385 367 346 109 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2187 2308 513 Size: 552 939 183 121 4353 Fragments arranged by size: 4353 939 552 183 121 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5409 Fragments arranged by size: 5409 683 56 BtsI GCAGTG_nn' Cuts at: 2140 2488 2809 3202 3228 3664 4458 4478 2140 Size: 348 321 393 26 436 794 20 3810 Fragments arranged by size: 3810 794 436 393 348 321 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6113 DraI TTT'AAA Cuts at: 3452 3452 Size: 6148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5155 809 Size: 507 3839 1802 Fragments arranged by size: 3839 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3019 3595 4484 Size: 54 1425 50 275 18 1189 576 889 Cuts at: 4484 6113 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1189 889 576 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 1537 Size: 275 1783 4090 Fragments arranged by size: 4090 1783 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2423 2821 3011 3313 4078 1353 Size: 1070 398 190 302 765 3423 Fragments arranged by size: 3423 1070 765 398 302 190 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2078 2084 4735 5109 5255 387 Size: 1064 138 489 6 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 489 374 146 138 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2307 2393 2823 3020 4211 4808 5595 Size: 393 675 86 430 197 1191 597 787 Cuts at: 5595 5863 1239 Size: 268 1524 Fragments arranged by size: 1524 1191 787 675 597 430 393 268 197 86 EcoNI CCTnn'n_nnAGG Cuts at: 2769 2769 Size: 6148 EcoO109I rG'GnC_Cy Cuts at: 2026 2462 2969 3025 3176 3237 2026 Size: 436 507 56 151 61 4937 Fragments arranged by size: 4937 507 436 151 61 56 EcoRI G'AATT_C Cuts at: 3457 3835 3457 Size: 378 5770 EcoRV GAT'ATC Cuts at: 2182 2182 Size: 6148 FspI TGC'GCA Cuts at: 4654 4654 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 3173 5295 1678 Size: 1495 2122 2531 Fragments arranged by size: 2531 2122 1495 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2424 2456 3311 3343 4789 Size: 32 135 32 852 32 855 32 1446 Cuts at: 4789 4821 4863 4895 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1446 855 852 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3492 3738 669 Size: 988 281 1554 246 3079 Fragments arranged by size: 3079 1554 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6148 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KasI G'GCGC_C Cuts at: 3169 3169 Size: 6148 KpnI G_GTAC'C Cuts at: 3473 3473 Size: 6148 MfeI C'AATT_G Cuts at: 3747 3747 Size: 6148 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3452 5262 5446 1305 Size: 2147 1810 184 2007 Fragments arranged by size: 2147 2007 1810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3021 10 Size: 54 1425 343 1189 3137 Fragments arranged by size: 3137 1425 1189 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2174 2495 4106 4465 4624 518 Size: 648 843 165 321 1611 359 159 2042 Fragments arranged by size: 2042 1611 843 648 359 321 165 159 MspA1I CmG'CkG Cuts at: 1606 2052 2843 2984 3133 3265 3365 3564 4241 Size: 446 791 141 149 132 100 199 677 Cuts at: 4241 5389 5634 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 791 677 446 245 199 149 141 132 100 NarI GG'CG_CC Cuts at: 3170 3170 Size: 6148 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4657 Fragments arranged by size: 4657 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NspI r_CATG'y Cuts at: 1132 1229 3506 5051 1132 Size: 97 2277 1545 2229 Fragments arranged by size: 2277 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6148 PciI A'CATG_T Cuts at: 5047 5047 Size: 6148 PflMI CCAn_nnn'nTGG Cuts at: 1108 3233 1108 Size: 2125 4023 PfoI T'CCnGG_A Cuts at: 2409 2409 Size: 6148 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4217 4249 5730 5762 4217 Size: 32 1481 32 4603 Fragments arranged by size: 4603 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2969 3025 2969 Size: 56 6092 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6148 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PspOMI G'GGCC_C Cuts at: 2026 2032 2462 3176 2026 Size: 6 430 714 4998 Fragments arranged by size: 4998 714 430 6 PspXI vC'TCGA_Gb Cuts at: 2581 2581 Size: 6148 PstI C_TGCA'G Cuts at: 830 2123 2881 2989 3500 830 Size: 1293 758 108 511 3478 Fragments arranged by size: 3478 1293 758 511 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 PvuII CAG'CTG Cuts at: 2843 3365 2843 Size: 522 5626 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SanDI GG'GwC_CC Cuts at: 2969 2969 Size: 6148 SapI GCTCTTCn'nnn_ Cuts at: 2821 3313 2821 Size: 492 5656 SbfI CC_TGCA'GG Cuts at: 2881 3500 2881 Size: 619 5529 ScaI AGT'ACT Cuts at: 2671 4396 6108 2671 Size: 1725 1712 2711 Fragments arranged by size: 2711 1725 1712 SfcI C'TryA_G Cuts at: 826 1045 2119 2877 2985 3496 4631 5312 5503 Size: 219 1074 758 108 511 1135 681 191 Cuts at: 5503 826 Size: 1471 Fragments arranged by size: 1471 1135 1074 758 681 511 219 191 108 SfoI GGC'GCC Cuts at: 3171 3171 Size: 6148 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 2031 2037 2974 3475 1872 Size: 159 6 937 501 4545 Fragments arranged by size: 4545 937 501 159 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 2581 4252 5153 5415 820 Size: 197 926 638 1671 901 262 1553 Fragments arranged by size: 1671 1553 926 901 638 262 197 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SrfI GCCC'GGGC Cuts at: 2031 2037 2031 Size: 6 6142 SspI AAT'ATT Cuts at: 5 52 4072 5 Size: 47 4020 2081 Fragments arranged by size: 4020 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6148 StyI C'CwwG_G Cuts at: 513 1065 2004 2770 3587 513 Size: 552 939 766 817 3074 Fragments arranged by size: 3074 939 817 766 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2102 2531 3244 4175 4192 4345 4530 Size: 1255 522 429 713 931 17 153 185 Cuts at: 4530 4975 325 Size: 445 1498 Fragments arranged by size: 1498 1255 931 713 522 445 429 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2669 4394 6106 Size: 274 80 33 51 1443 692 1725 1712 Cuts at: 6106 96 Size: 138 Fragments arranged by size: 1725 1712 1443 692 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2075 2107 2961 2993 3181 3213 3252 3284 6108 Size: 32 854 32 188 32 39 32 2824 Cuts at: 6108 6140 2075 Size: 32 2083 Fragments arranged by size: 2824 2083 854 188 39 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2453 1316 Size: 1137 5011 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3442 3442 Size: 6148 XhoI C'TCGA_G Cuts at: 1943 2581 1943 Size: 638 5510 XmaI C'CCGG_G Cuts at: 1870 2029 2035 2972 3473 1870 Size: 159 6 937 501 4545 Fragments arranged by size: 4545 937 501 159 6 XmnI GAAnn'nnTTC Cuts at: 4277 4277 Size: 6148 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SexAI SfiI SwaI