(Circular) (Six-base) MAPSORT of: pFN21AB5829.seq Check: 6978 from: 1 to: 5077 pFN21AB5829 5077 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2887 278 Size: 53 83 186 936 1351 2468 Fragments arranged by size: 2468 1351 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2420 1937 Size: 483 4594 Acc65I G'GTAC_C Cuts at: 2398 2398 Size: 5077 AclI AA'CG_TT Cuts at: 1563 3204 3577 1563 Size: 1641 373 3063 Fragments arranged by size: 3063 1641 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2014 3140 4524 4792 1632 Size: 382 1126 1384 268 1917 Fragments arranged by size: 1917 1384 1126 382 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4880 AflIII A'CryG_T Cuts at: 1203 3976 4953 1203 Size: 2773 977 1327 Fragments arranged by size: 2773 1327 977 AgeI A'CCGG_T Cuts at: 3908 3908 Size: 5077 AhdI GACnn_n'nnGTC Cuts at: 3806 3806 Size: 5077 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5077 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5077 AlwNI CAG_nnn'CTG Cuts at: 2074 2240 2820 4392 2074 Size: 166 580 1572 2759 Fragments arranged by size: 2759 1572 580 166 ApaLI G'TGCA_C Cuts at: 3134 4290 3134 Size: 1156 3921 ApoI r'AATT_y Cuts at: 1493 2386 2612 2764 4873 1493 Size: 893 226 152 2109 1697 Fragments arranged by size: 2109 1697 893 226 152 AseI AT'TA_AT Cuts at: 160 1032 3631 160 Size: 872 2599 1606 Fragments arranged by size: 2599 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5077 AvaI C'yCGr_G Cuts at: 1870 1943 2402 1870 Size: 73 459 4545 Fragments arranged by size: 4545 459 73 BamHI G'GATC_C Cuts at: 2247 2407 2774 2247 Size: 160 367 4550 Fragments arranged by size: 4550 367 160 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2168 2187 2398 3853 618 Size: 325 217 627 381 19 211 1455 1842 Fragments arranged by size: 1842 1455 627 381 325 217 211 19 BanII G_rGCy'C Cuts at: 1391 2125 2340 2396 1391 Size: 734 215 56 4072 Fragments arranged by size: 4072 734 215 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4106 BbvCI CC'TCA_GC Cuts at: 2233 2233 Size: 5077 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3268 3302 1903 Size: 34 1331 34 3678 Fragments arranged by size: 3678 1331 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2969 4179 2969 Size: 1210 3867 BclI T'GATC_A Cuts at: 1548 2347 1548 Size: 799 4278 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3688 136 Size: 107 122 71 631 605 2016 1525 Fragments arranged by size: 2016 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2033 5072 2033 Size: 3039 2038 BlpI GC'TnA_GC Cuts at: 2339 2494 2339 Size: 155 4922 Bme1580I G_kGCm'C Cuts at: 1790 2173 3138 4294 1790 Size: 383 965 1156 2573 Fragments arranged by size: 2573 1156 965 383 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5077 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2330 2808 3766 Size: 416 53 83 399 24 913 478 958 Cuts at: 3766 442 Size: 1753 Fragments arranged by size: 1753 958 913 478 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5077 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2260 2313 3737 1239 Size: 1021 53 1424 2579 Fragments arranged by size: 2579 1424 1021 53 Bpu10I CC'TnA_GC Cuts at: 2233 2233 Size: 5077 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2025 3202 4067 4365 2025 Size: 1177 865 298 2737 Fragments arranged by size: 2737 1177 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2079 2276 3740 882 Size: 617 580 197 1464 2219 Fragments arranged by size: 2219 1464 617 580 197 BsaAI yAC'GTr Cuts at: 493 1204 2868 493 Size: 711 1664 2702 Fragments arranged by size: 2702 1664 711 BsaBI GATnn'nnATC Cuts at: 5071 5071 Size: 5077 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2884 3266 3901 275 Size: 53 83 186 936 1351 382 635 1451 Fragments arranged by size: 1451 1351 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3510 3908 4182 4329 4740 3510 Size: 398 274 147 411 3847 Fragments arranged by size: 3847 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2286 2316 1178 Size: 30 165 30 883 30 3939 Fragments arranged by size: 3939 883 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5077 BseYI C'CCAG_C Cuts at: 2142 4280 4776 2142 Size: 2138 496 2443 Fragments arranged by size: 2443 2138 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4504 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2527 3288 4316 1540 Size: 275 186 526 761 1028 2301 Fragments arranged by size: 2301 1028 761 526 275 186 BsiHKAI G_wGCw'C Cuts at: 2340 3138 3223 4294 2340 Size: 798 85 1071 3123 Fragments arranged by size: 3123 1071 798 85 BsmI GAATG_Cn' Cuts at: 2588 2681 2588 Size: 93 4984 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2125 2173 2340 2396 3138 3223 4294 Size: 399 335 48 167 56 742 85 1071 Cuts at: 4294 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 335 167 85 56 48 BspEI T'CCGG_A Cuts at: 4740 4740 Size: 5077 BspHI T'CATG_A Cuts at: 2966 4696 2966 Size: 1730 3347 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2365 2432 844 Size: 1521 67 3489 Fragments arranged by size: 3489 1521 67 BsrBI CCG'CTC Cuts at: 1394 1823 2964 1394 Size: 429 1141 3507 Fragments arranged by size: 3507 1141 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3572 3746 65 Size: 1183 2324 174 1396 Fragments arranged by size: 2324 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3721 3908 1700 Size: 110 1911 187 2869 Fragments arranged by size: 2869 1911 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5077 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5077 BssSI C'ACGA_G Cuts at: 3137 4149 3137 Size: 1012 4065 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5077 BstBI TT'CG_AA Cuts at: 3881 3881 Size: 5077 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5077 BstYI r'GATC_y Cuts at: 1972 2009 2033 2152 2222 2247 2407 2774 3159 Size: 37 24 119 70 25 160 367 385 Cuts at: 3159 3176 4628 4714 4726 5072 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 160 119 86 70 37 25 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3586 Fragments arranged by size: 3586 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4338 Fragments arranged by size: 4338 683 56 BtsI GCAGTG_nn' Cuts at: 2059 2593 3387 3407 2059 Size: 534 794 20 3729 Fragments arranged by size: 3729 794 534 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5077 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2287 2322 529 Size: 35 1723 35 3284 Fragments arranged by size: 3284 1723 35 35 DraI TTT'AAA Cuts at: 2381 2381 Size: 5077 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4084 809 Size: 507 2768 1802 Fragments arranged by size: 2768 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2524 3413 5042 Size: 54 1425 50 275 18 694 889 1629 Cuts at: 5042 8 Size: 43 Fragments arranged by size: 1629 1425 889 694 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2524 1537 Size: 275 712 4090 Fragments arranged by size: 4090 712 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3007 1353 Size: 1654 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3664 4038 4184 387 Size: 1064 138 2075 374 146 1280 Fragments arranged by size: 2075 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2338 2338 Size: 5077 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2014 2260 2313 3140 3737 4524 4792 Size: 393 382 246 53 827 597 787 268 Cuts at: 4792 1239 Size: 1524 Fragments arranged by size: 1524 827 787 597 393 382 268 246 53 EcoO109I rG'GnC_Cy Cuts at: 2071 2071 Size: 5077 EcoRI G'AATT_C Cuts at: 2386 2764 2386 Size: 378 4699 FspI TGC'GCA Cuts at: 3583 3583 Size: 5077 HaeII r_GCGC'y Cuts at: 1678 4224 1678 Size: 2546 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3718 3750 3792 3824 1373 Size: 32 135 32 2146 32 42 32 2626 Fragments arranged by size: 2626 2146 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2421 2667 669 Size: 988 281 483 246 3079 Fragments arranged by size: 3079 988 483 281 246 HindIII A'AGCT_T Cuts at: 748 2043 748 Size: 1295 3782 HpaI GTT'AAC Cuts at: 2667 2667 Size: 5077 KpnI G_GTAC'C Cuts at: 2402 2402 Size: 5077 MfeI C'AATT_G Cuts at: 2676 2676 Size: 5077 MluI A'CGCG_T Cuts at: 4953 4953 Size: 5077 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4191 4375 1305 Size: 2886 184 2007 Fragments arranged by size: 2886 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3255 Fragments arranged by size: 3255 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3035 3394 3553 518 Size: 648 1869 359 159 2042 Fragments arranged by size: 2042 1869 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2493 3170 4318 4563 1606 Size: 887 677 1148 245 2120 Fragments arranged by size: 2120 1148 887 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3586 Fragments arranged by size: 3586 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5077 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5077 NotI GC'GGCC_GC Cuts at: 2524 2524 Size: 5077 NspI r_CATG'y Cuts at: 1132 1229 2435 3980 1132 Size: 97 1206 1545 2229 Fragments arranged by size: 2229 1545 1206 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5077 PciI A'CATG_T Cuts at: 3976 3976 Size: 5077 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5077 PfoI T'CCnGG_A Cuts at: 2290 2290 Size: 5077 PmeI GTTT'AAAC Cuts at: 2381 2381 Size: 5077 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3146 3178 4659 4691 3146 Size: 32 1481 32 3532 Fragments arranged by size: 3532 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5077 PsiI TTA'TAA Cuts at: 2647 2647 Size: 5077 PstI C_TGCA'G Cuts at: 830 2054 2429 830 Size: 1224 375 3478 Fragments arranged by size: 3478 1224 375 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5077 SacI G_AGCT'C Cuts at: 2340 2340 Size: 5077 SalI G'TCGA_C Cuts at: 1936 2419 1936 Size: 483 4594 SbfI CC_TGCA'GG Cuts at: 2429 2429 Size: 5077 ScaI AGT'ACT Cuts at: 3325 5037 3325 Size: 1712 3365 SfcI C'TryA_G Cuts at: 826 1045 2050 2425 3560 4241 4432 826 Size: 219 1005 375 1135 681 191 1471 Fragments arranged by size: 1471 1135 1005 681 375 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5077 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5077 SmaI CCC'GGG Cuts at: 1872 2404 1872 Size: 532 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2040 3181 4082 4344 820 Size: 197 926 97 1141 901 262 1553 Fragments arranged by size: 1553 1141 926 901 262 197 97 SnaBI TAC'GTA Cuts at: 493 2868 493 Size: 2375 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5077 SphI G_CATG'C Cuts at: 1132 2435 1132 Size: 1303 3774 SspI AAT'ATT Cuts at: 5 52 3001 5 Size: 47 2949 2081 Fragments arranged by size: 2949 2081 47 StuI AGG'CCT Cuts at: 1517 2259 2281 1517 Size: 742 22 4313 Fragments arranged by size: 4313 742 22 StyI C'CwwG_G Cuts at: 513 1065 2004 2260 2516 513 Size: 552 939 256 256 3074 Fragments arranged by size: 3074 939 552 256 256 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2156 3104 3121 3274 3459 3904 325 Size: 1255 576 948 17 153 185 445 1498 Fragments arranged by size: 1498 1255 948 576 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3323 5035 96 Size: 274 80 33 51 1443 1346 1712 138 Fragments arranged by size: 1712 1443 1346 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2286 2318 5037 5069 2286 Size: 32 2719 32 2294 Fragments arranged by size: 2719 2294 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5077 XbaI T'CTAG_A Cuts at: 2413 2413 Size: 5077 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5077 XmaI C'CCGG_G Cuts at: 1870 2402 1870 Size: 532 4545 XmnI GAAnn'nnTTC Cuts at: 3206 3206 Size: 5077 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2885 276 Size: 53 83 186 936 1351 2468 Fragments arranged by size: 2468 1351 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI