################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglII BlnI BpuAI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MfeI MluI MroI MslI MunI MvnI NarI NciI NdeI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AciI 1326 GCGG CCGC(-3/-1) AfaI 402 GTAC GT^AC AflIII 850 ACATGT A^C[GA][CT]GT Aor51HI 167 AGCGCT AGC^GCT AspHI 679 GAGCTC G[AT]GC[AT]^C AviII 1088 TGCGCA TGC^GCA BalI 93 TGGCCA TGG^CCA BanII 679 GAGCTC G[GA]GC[CT]^C BbvCI 843 GCTGAGG CCTCAGC(-5/-2) BglI 646 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 713 GCTCAGC GC^T[ACGT]AGC Bpu1102I 713 GCTCAGC GC^T[ACGT]AGC BsaBI 976 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1251 GGTCTC GGTCTC(1/5) BsiHKAI 679 GAGCTC G[AT]GC[AT]^C BsmI 112 GAATGC GAATGC(1/-1) BspLU11I 850 ACATGT A^CATGT BsrFI 161 GCCGGC [GA]^CCGG[CT] BstAPI 1101 GCACTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 606 GGATG GGATG(2/0) CelII 713 GCTCAGC GC^T[ACGT]AGC Cfr10I 161 GCCGGC [GA]^CCGG[CT] DrdI 1063 GACCTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 93 TGGCCA [CT]^GGCC[GA] EarI 964 GAAGAG CTCTTC(1/4) Eco47III 167 AGCGCT AGC^GCT EcoT22I 114 ATGCAT ATGCA^T FokI 606 GGATG GGATG(9/13) FspI 1088 TGCGCA TGC^GCA HaeII 167 AGCGCT [GA]GCGC^[CT] HgaI 146 GCGTC GACGC(5/10) Ksp632I 964 GAAGAG CTCTTC(1/4) MamI 976 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 93 TGGCCA TGG^CCA MscI 93 TGGCCA TGG^CCA MseI 227 TTAA T^TAA NaeI 161 GCCGGC GCC^GGC NgoMIV 161 GCCGGC G^CCGGC NheI 204 GCTAGC G^CTAGC NsiI 114 ATGCAT ATGCA^T RsaI 402 GTAC GT^AC SacI 679 GAGCTC GAGCT^C SfiI 645 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspI 1396 AATATT AAT^ATT StuI 1055 AGGCCT AGG^CCT Tru9I 227 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BamHI 873 GGATCC G^GATCC 1411 GGATCC BseRI 34 GAGGAG GAGGAG(10/8) 385 GAGGAG BsmFI 1061 GGGAC GGGAC(10/14) 376 GTCCC BstYI 873 GGATCC [GA]^GATC[CT] 1411 GGATCC CfoI 168 GCGC GCG^C 1089 GCGC EcoT14I 371 CCATGG C^C[AT][AT]GG 1275 CCATGG HapII 162 CCGG C^CGG 189 CCGG HhaI 168 GCGC GCG^C 1089 GCGC HinP1I 168 GCGC G^CGC 1089 GCGC HpaII 162 CCGG C^CGG 189 CCGG MflI 873 GGATCC [GA]^GATC[CT] 1411 GGATCC MspA1I 586 CAGCTG C[AC]G^C[GT]G 619 CAGCTG MspI 162 CCGG C^CGG 189 CCGG NcoI 371 CCATGG C^CATGG 1275 CCATGG NspI 43 ACATGC [GA]CATG^[CT] 850 ACATGT PflMI 366 CCACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 887 CCAATCCGTGG PvuII 586 CAGCTG CAG^CTG 619 CAGCTG SmlI 178 CTCAAG C^T[CT][GA]AG 382 CTTGAG StyI 371 CCATGG C^C[AT][AT]GG 1275 CCATGG Van91I 366 CCACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 887 CCAATCCGTGG XhoII 873 GGATCC [GA]^GATC[CT] 1411 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AluI AlwI AlwNI AvaII BanI BbvI BfaI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PstI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I