(Circular) (Six-base) MAPSORT of: pFN21AB5826.seq Check: 6281 from: 1 to: 6130 pFN21AB5826 6130 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3473 1937 Size: 1536 4594 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1995 2801 2858 3188 3325 4193 5577 5845 Size: 363 806 57 330 137 868 1384 268 Cuts at: 5845 1632 Size: 1917 Fragments arranged by size: 1917 1384 868 806 363 330 268 137 57 AfeI AGC'GCT Cuts at: 2174 2174 Size: 6130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 2855 5029 6006 1203 Size: 1652 2174 977 1327 Fragments arranged by size: 2174 1652 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6130 AlwNI CAG_nnn'CTG Cuts at: 2109 2154 2602 3873 5445 2109 Size: 45 448 1271 1572 2794 Fragments arranged by size: 2794 1572 1271 448 45 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 3439 3665 3817 5926 1493 Size: 1946 226 152 2109 1697 Fragments arranged by size: 2109 1946 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 3455 1870 Size: 73 1512 4545 Fragments arranged by size: 4545 1512 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2390 2423 2390 Size: 33 6097 BamHI G'GATC_C Cuts at: 2878 3416 3460 3827 2878 Size: 538 44 367 5181 Fragments arranged by size: 5181 538 367 44 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2709 3002 3176 3208 3451 Size: 325 217 627 922 293 174 32 243 Cuts at: 3451 4906 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 922 627 325 293 243 217 174 32 BanII G_rGCy'C Cuts at: 1391 2688 3449 1391 Size: 1297 761 4072 Fragments arranged by size: 4072 1297 761 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5159 BbvCI CC'TCA_GC Cuts at: 2849 2849 Size: 6130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BfrBI ATG'CAT Cuts at: 2121 2121 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2657 4741 136 Size: 107 122 71 631 605 985 2084 1525 Fragments arranged by size: 2084 1525 985 631 605 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 2719 3547 2719 Size: 828 5302 Bme1580I G_kGCm'C Cuts at: 1790 2264 2350 3007 3108 4191 5347 1790 Size: 474 86 657 101 1083 1156 2573 Fragments arranged by size: 2573 1156 1083 657 474 101 86 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2344 3043 3861 Size: 416 53 83 399 24 927 699 818 Cuts at: 3861 4819 442 Size: 958 1753 Fragments arranged by size: 1753 958 927 818 699 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2213 1056 Size: 1157 4973 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2707 2707 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 2624 2787 4790 1239 Size: 1166 219 163 2003 2579 Fragments arranged by size: 2579 2003 1166 219 163 Bpu10I CC'TnA_GC Cuts at: 2849 2849 Size: 6130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2168 2408 4255 5120 5418 2168 Size: 240 1847 865 298 2880 Fragments arranged by size: 2880 1847 865 298 240 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3262 4793 882 Size: 617 1763 1531 2219 Fragments arranged by size: 2219 1763 1531 617 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 2985 6124 2985 Size: 3139 2991 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 4319 4954 275 Size: 53 83 186 936 2404 382 635 1451 Fragments arranged by size: 2404 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4563 4961 5235 5382 5793 4563 Size: 398 274 147 411 4900 Fragments arranged by size: 4900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3269 3299 1178 Size: 30 165 30 1866 30 4009 Fragments arranged by size: 4009 1866 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2405 1186 Size: 868 351 4911 Fragments arranged by size: 4911 868 351 BseYI C'CCAG_C Cuts at: 2337 2661 2946 3006 5333 5829 2337 Size: 324 285 60 2327 496 2638 Fragments arranged by size: 2638 2327 496 324 285 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2301 2382 2573 2880 1163 Size: 573 565 81 191 307 4413 Fragments arranged by size: 4413 573 565 307 191 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2688 4191 4276 5347 2688 Size: 1503 85 1071 3471 Fragments arranged by size: 3471 1503 1071 85 BsmI GAATG_Cn' Cuts at: 2123 3641 3734 2123 Size: 1518 93 4519 Fragments arranged by size: 4519 1518 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2264 2350 2688 3007 3108 3449 4191 Size: 399 474 86 338 319 101 341 742 Cuts at: 4191 4276 5347 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 474 399 341 338 319 101 86 85 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4017 1394 Size: 429 2194 3507 Fragments arranged by size: 3507 2194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4625 4799 65 Size: 1183 3377 174 1396 Fragments arranged by size: 3377 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2166 4774 4961 1700 Size: 110 356 2608 187 2869 Fragments arranged by size: 2869 2608 356 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6130 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 3112 794 Size: 2318 3812 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstYI r'GATC_y Cuts at: 1972 2878 3416 3460 3827 4212 4229 5681 5767 Size: 906 538 44 367 385 17 1452 86 Cuts at: 5767 5779 6125 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 906 538 385 367 346 86 44 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2376 2897 3280 513 Size: 552 939 372 521 383 3363 Fragments arranged by size: 3363 939 552 521 383 372 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 2289 3646 4440 4460 2289 Size: 1357 794 20 3959 Fragments arranged by size: 3959 1357 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6095 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3074 5137 809 Size: 507 1758 2063 1802 Fragments arranged by size: 2063 1802 1758 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 3577 4466 Size: 54 1425 50 275 18 268 1479 889 Cuts at: 4466 6095 8 Size: 1629 43 Fragments arranged by size: 1629 1479 1425 889 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2964 4060 1353 Size: 1611 1096 3423 Fragments arranged by size: 3423 1611 1096 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2686 2686 Size: 6130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1995 2405 2624 2787 2801 2858 3188 Size: 393 363 410 219 163 14 57 330 Cuts at: 3188 3325 4193 4790 5577 5845 1239 Size: 137 868 597 787 268 1524 Fragments arranged by size: 1524 868 787 597 410 393 363 330 268 219 163 137 57 14 EcoO109I rG'GnC_Cy Cuts at: 2329 2674 2785 3067 3323 2329 Size: 345 111 282 256 5136 Fragments arranged by size: 5136 345 282 256 111 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2951 3124 3148 2951 Size: 173 24 5933 Fragments arranged by size: 5933 173 24 FspI TGC'GCA Cuts at: 3095 4636 3095 Size: 1541 4589 HaeII r_GCGC'y Cuts at: 1678 2176 5277 1678 Size: 498 3101 2531 Fragments arranged by size: 3101 2531 498 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2783 2815 3269 3301 4771 Size: 32 135 32 1211 32 454 32 1470 Cuts at: 4771 4803 4845 4877 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1470 1211 454 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3474 3720 669 Size: 988 281 1536 246 3079 Fragments arranged by size: 3079 1536 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6130 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2451 5244 5428 1305 Size: 1146 2793 184 2007 Fragments arranged by size: 2793 2007 1146 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2100 10 Size: 54 1425 343 268 4040 Fragments arranged by size: 4040 1425 343 268 54 MslI CAynn'nnrTG Cuts at: 518 1166 4088 4447 4606 518 Size: 648 2922 359 159 2042 Fragments arranged by size: 2922 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2593 2626 3546 4223 5371 5616 1606 Size: 987 33 920 677 1148 245 2120 Fragments arranged by size: 2120 1148 987 920 677 245 33 NaeI GCC'GGC Cuts at: 2168 2168 Size: 6130 NcoI C'CATG_G Cuts at: 513 1065 2004 2376 3280 513 Size: 552 939 372 904 3363 Fragments arranged by size: 3363 939 904 552 372 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NgoMIV G'CCGG_C Cuts at: 2166 2166 Size: 6130 NheI G'CTAG_C Cuts at: 1052 2209 1052 Size: 1157 4973 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NsiI A_TGCA'T Cuts at: 2123 2123 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2052 2859 3488 5033 1132 Size: 97 823 807 629 1545 2229 Fragments arranged by size: 2229 1545 823 807 629 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 2855 5029 2855 Size: 2174 3956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 2898 1108 Size: 1269 521 4340 Fragments arranged by size: 4340 1269 521 PfoI T'CCnGG_A Cuts at: 2754 2771 3234 2754 Size: 17 463 5650 Fragments arranged by size: 5650 463 17 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2785 3067 3323 2785 Size: 282 256 5592 Fragments arranged by size: 5592 282 256 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PstI C_TGCA'G Cuts at: 830 2598 2739 3083 3482 830 Size: 1768 141 344 399 3478 Fragments arranged by size: 3478 1768 399 344 141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 2593 2626 2593 Size: 33 6097 SacI G_AGCT'C Cuts at: 2688 2688 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SfcI C'TryA_G Cuts at: 826 1045 2594 2678 2735 3079 3478 4613 5294 Size: 219 1549 84 57 344 399 1135 681 Cuts at: 5294 5485 826 Size: 191 1471 Fragments arranged by size: 1549 1471 1135 681 399 344 219 191 84 57 SfiI GGCCn_nnn'nGGCC Cuts at: 2657 2657 Size: 6130 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2183 2387 4234 5135 5397 820 Size: 197 926 240 204 1847 901 262 1553 Fragments arranged by size: 1847 1553 926 901 262 240 204 197 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 3403 4054 5 Size: 47 3351 651 2081 Fragments arranged by size: 3351 2081 651 47 StuI AGG'CCT Cuts at: 1517 3062 1517 Size: 1545 4585 StyI C'CwwG_G Cuts at: 513 1065 2004 2376 3280 3569 513 Size: 552 939 372 904 289 3074 Fragments arranged by size: 3074 939 904 552 372 289 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2960 2990 3062 4157 4174 4327 4512 Size: 1255 1380 30 72 1095 17 153 185 Cuts at: 4512 4957 325 Size: 445 1498 Fragments arranged by size: 1498 1380 1255 1095 445 185 153 72 30 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4376 6088 96 Size: 274 80 33 51 1443 2399 1712 138 Fragments arranged by size: 2399 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2350 2382 2863 2878 2895 2910 6090 6122 2350 Size: 32 481 15 17 15 3180 32 2358 Fragments arranged by size: 3180 2358 481 32 32 17 15 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI