(Circular) (Six-base) MAPSORT of: pFN21AB9973.seq Check: 9335 from: 1 to: 6367 pFN21AB9973 6367 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2154 2783 3510 2154 Size: 629 727 5011 Fragments arranged by size: 5011 727 629 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4177 278 Size: 53 83 186 936 2641 2468 Fragments arranged by size: 2641 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2214 3710 1937 Size: 277 1496 4594 Fragments arranged by size: 4594 1496 277 Acc65I G'GTAC_C Cuts at: 3688 3688 Size: 6367 AclI AA'CG_TT Cuts at: 1563 4494 4867 1563 Size: 2931 373 3063 Fragments arranged by size: 3063 2931 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2284 2381 4430 5814 6082 1632 Size: 652 97 2049 1384 268 1917 Fragments arranged by size: 2049 1917 1384 652 268 97 AflII C'TTAA_G Cuts at: 820 1017 2249 820 Size: 197 1232 4938 Fragments arranged by size: 4938 1232 197 AflIII A'CryG_T Cuts at: 1203 5266 6243 1203 Size: 4063 977 1327 Fragments arranged by size: 4063 1327 977 AgeI A'CCGG_T Cuts at: 5198 5198 Size: 6367 AhdI GACnn_n'nnGTC Cuts at: 5096 5096 Size: 6367 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6367 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3323 1184 Size: 2139 4228 AlwNI CAG_nnn'CTG Cuts at: 2155 2164 2440 2725 4110 5682 2155 Size: 9 276 285 1385 1572 2840 Fragments arranged by size: 2840 1572 1385 285 276 9 ApaI G_GGCC'C Cuts at: 3119 3119 Size: 6367 ApaLI G'TGCA_C Cuts at: 4424 5580 4424 Size: 1156 5211 ApoI r'AATT_y Cuts at: 1493 3676 3902 4054 6163 1493 Size: 2183 226 152 2109 1697 Fragments arranged by size: 2183 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4921 160 Size: 872 3889 1606 Fragments arranged by size: 3889 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6367 AvaI C'yCGr_G Cuts at: 1870 1943 2870 3295 3692 1870 Size: 73 927 425 397 4545 Fragments arranged by size: 4545 927 425 397 73 BamHI G'GATC_C Cuts at: 2737 3464 3697 4064 2737 Size: 727 233 367 5040 Fragments arranged by size: 5040 727 367 233 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 3508 3688 5143 618 Size: 325 217 627 231 1490 180 1455 1842 Fragments arranged by size: 1842 1490 1455 627 325 231 217 180 BanII G_rGCy'C Cuts at: 1391 2057 3015 3119 3359 3686 1391 Size: 666 958 104 240 327 4072 Fragments arranged by size: 4072 958 666 327 240 104 BbeI G_GCGC'C Cuts at: 2022 2022 Size: 6367 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3167 3487 928 Size: 971 1268 320 3808 Fragments arranged by size: 3808 1268 971 320 BbvCI CC'TCA_GC Cuts at: 3242 3242 Size: 6367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4558 4592 1903 Size: 34 2621 34 3678 Fragments arranged by size: 3678 2621 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4259 5469 4259 Size: 1210 5157 BclI T'GATC_A Cuts at: 1548 1548 Size: 6367 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2657 3237 4978 Size: 107 122 71 631 605 985 580 1741 Cuts at: 4978 136 Size: 1525 Fragments arranged by size: 1741 1525 985 631 605 580 122 107 71 BglII A'GATC_T Cuts at: 6362 6362 Size: 6367 BlpI GC'TnA_GC Cuts at: 3267 3784 3267 Size: 517 5850 Bme1580I G_kGCm'C Cuts at: 1790 2906 3119 3513 4428 5584 1790 Size: 1116 213 394 915 1156 2573 Fragments arranged by size: 2573 1156 1116 915 394 213 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6367 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2798 3507 4098 Size: 416 53 83 399 24 1381 709 591 Cuts at: 4098 5056 442 Size: 958 1753 Fragments arranged by size: 1753 1381 958 709 591 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6367 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2716 3223 3484 2716 Size: 507 261 5599 Fragments arranged by size: 5599 507 261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 2462 2524 2952 2965 3344 3454 3467 Size: 1178 45 62 428 13 379 110 13 Cuts at: 3467 5027 1239 Size: 1560 2579 Fragments arranged by size: 2579 1560 1178 428 379 110 62 45 13 13 Bpu10I CC'TnA_GC Cuts at: 3242 3242 Size: 6367 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4492 5357 5655 4492 Size: 865 298 5204 Fragments arranged by size: 5204 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2177 2792 3183 5030 882 Size: 617 678 615 391 1847 2219 Fragments arranged by size: 2219 1847 678 617 615 391 BsaAI yAC'GTr Cuts at: 493 1204 4158 493 Size: 711 2954 2702 Fragments arranged by size: 2954 2702 711 BsaBI GATnn'nnATC Cuts at: 6361 6361 Size: 6367 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 4174 4556 5191 Size: 53 83 186 936 486 2155 382 635 Cuts at: 5191 275 Size: 1451 Fragments arranged by size: 2155 1451 936 635 486 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4800 5198 5472 5619 6030 4800 Size: 398 274 147 411 5137 Fragments arranged by size: 5137 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2174 2204 1178 Size: 30 165 30 771 30 5341 Fragments arranged by size: 5341 771 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 3283 3349 3367 1186 Size: 931 1166 66 18 4186 Fragments arranged by size: 4186 1166 931 66 18 BseYI C'CCAG_C Cuts at: 2050 3023 5570 6066 2050 Size: 973 2547 496 2351 Fragments arranged by size: 2547 2351 973 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2354 2711 1163 Size: 573 618 357 4819 Fragments arranged by size: 4819 618 573 357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3817 4578 5606 1540 Size: 275 186 1816 761 1028 2301 Fragments arranged by size: 2301 1816 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2774 3341 4428 4513 5584 2774 Size: 567 1087 85 1071 3557 Fragments arranged by size: 3557 1087 1071 567 85 BsmI GAATG_Cn' Cuts at: 2540 3878 3971 2540 Size: 1338 93 4936 Fragments arranged by size: 4936 1338 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2774 2906 3015 3119 3341 3359 Size: 399 267 717 132 109 104 222 18 Cuts at: 3359 3513 3686 4428 4513 5584 1391 Size: 154 173 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 717 399 267 222 173 154 132 109 104 85 18 BspEI T'CCGG_A Cuts at: 6030 6030 Size: 6367 BspHI T'CATG_A Cuts at: 4256 5986 4256 Size: 1730 4637 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2154 2783 3510 3722 844 Size: 1310 629 727 212 3489 Fragments arranged by size: 3489 1310 727 629 212 BsrBI CCG'CTC Cuts at: 1394 1823 3596 4254 1394 Size: 429 1773 658 3507 Fragments arranged by size: 3507 1773 658 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2904 4862 5036 65 Size: 1183 1656 1958 174 1396 Fragments arranged by size: 1958 1656 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3079 5011 5198 1700 Size: 110 1269 1932 187 2869 Fragments arranged by size: 2869 1932 1269 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6367 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6367 BssSI C'ACGA_G Cuts at: 2188 4427 5439 2188 Size: 2239 1012 3116 Fragments arranged by size: 3116 2239 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2155 2514 2725 794 Size: 1361 359 211 4436 Fragments arranged by size: 4436 1361 359 211 BstBI TT'CG_AA Cuts at: 5171 5171 Size: 6367 BstEII G'GTnAC_C Cuts at: 1184 2554 1184 Size: 1370 4997 BstXI CCAn_nnnn'nTGG Cuts at: 2132 2132 Size: 6367 BstYI r'GATC_y Cuts at: 1972 2233 2737 3216 3464 3697 4064 4449 4466 Size: 261 504 479 248 233 367 385 17 Cuts at: 4466 5918 6004 6016 6362 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 504 479 385 367 346 261 248 233 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2646 513 Size: 552 1581 4234 Fragments arranged by size: 4234 1581 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2308 487 Size: 683 56 1082 4546 Fragments arranged by size: 4546 1082 683 56 BtsI GCAGTG_nn' Cuts at: 2608 2752 3224 3883 4677 4697 2608 Size: 144 472 659 794 20 4278 Fragments arranged by size: 4278 794 659 472 144 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6367 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6332 DraI TTT'AAA Cuts at: 3671 3671 Size: 6367 DraIII CAC_nnn'GTG Cuts at: 2734 2734 Size: 6367 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5374 809 Size: 507 4058 1802 Fragments arranged by size: 4058 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2759 3133 3534 Size: 54 1425 50 275 18 929 374 401 Cuts at: 3534 3814 4703 6332 8 Size: 280 889 1629 43 Fragments arranged by size: 1629 1425 929 889 401 374 280 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3814 1537 Size: 275 2002 4090 Fragments arranged by size: 4090 2002 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2357 2382 4297 1353 Size: 1004 25 1915 3423 Fragments arranged by size: 3423 1915 1004 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3586 4954 5328 5474 387 Size: 1064 138 1997 1368 374 146 1280 Fragments arranged by size: 1997 1368 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2284 2381 2417 2462 2524 2952 2965 Size: 393 652 97 36 45 62 428 13 Cuts at: 2965 3344 3454 3467 4430 5027 5814 6082 1239 Size: 379 110 13 963 597 787 268 1524 Fragments arranged by size: 1524 963 787 652 597 428 393 379 268 110 97 62 45 36 13 13 EcoNI CCTnn'n_nnAGG Cuts at: 2252 2486 3106 3635 2252 Size: 234 620 529 4984 Fragments arranged by size: 4984 620 529 234 EcoO109I rG'GnC_Cy Cuts at: 2859 2914 3116 3238 3402 2859 Size: 55 202 122 164 5824 Fragments arranged by size: 5824 202 164 122 55 EcoRI G'AATT_C Cuts at: 3676 4054 3676 Size: 378 5989 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2386 2476 2386 Size: 90 6277 FspI TGC'GCA Cuts at: 4873 4873 Size: 6367 HaeII r_GCGC'y Cuts at: 1678 2022 2032 5514 1678 Size: 344 10 3482 2531 Fragments arranged by size: 3482 2531 344 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 2158 2190 2597 Size: 32 135 32 521 32 33 32 407 Cuts at: 2597 2629 3154 3158 3186 3190 3440 3472 5008 Size: 32 525 4 28 4 250 32 1536 Cuts at: 5008 5040 5082 5114 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1536 525 521 407 250 135 42 33 32 32 32 32 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 3711 3957 669 Size: 988 281 1773 246 3079 Fragments arranged by size: 3079 1773 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6367 HpaI GTT'AAC Cuts at: 3957 3957 Size: 6367 KasI G'GCGC_C Cuts at: 2018 2018 Size: 6367 KpnI G_GTAC'C Cuts at: 3692 3692 Size: 6367 MfeI C'AATT_G Cuts at: 3966 3966 Size: 6367 MluI A'CGCG_T Cuts at: 6243 6243 Size: 6367 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 3520 5481 5665 1305 Size: 786 1429 1961 184 2007 Fragments arranged by size: 2007 1961 1429 786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3135 10 Size: 54 1425 343 1303 3242 Fragments arranged by size: 3242 1425 1303 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4325 4684 4843 518 Size: 648 3159 359 159 2042 Fragments arranged by size: 3159 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2161 2338 2758 3783 4460 5608 5853 1606 Size: 555 177 420 1025 677 1148 245 2120 Fragments arranged by size: 2120 1148 1025 677 555 420 245 177 NaeI GCC'GGC Cuts at: 3081 3081 Size: 6367 NarI GG'CG_CC Cuts at: 2019 2019 Size: 6367 NcoI C'CATG_G Cuts at: 513 1065 2646 513 Size: 552 1581 4234 Fragments arranged by size: 4234 1581 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6367 NgoMIV G'CCGG_C Cuts at: 3079 3079 Size: 6367 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6367 NotI GC'GGCC_GC Cuts at: 3814 3814 Size: 6367 NspI r_CATG'y Cuts at: 1132 1229 3004 3725 5270 1132 Size: 97 1775 721 1545 2229 Fragments arranged by size: 2229 1775 1545 721 97 PasI CC'CwG_GG Cuts at: 1348 3108 3235 1348 Size: 1760 127 4480 Fragments arranged by size: 4480 1760 127 PciI A'CATG_T Cuts at: 5266 5266 Size: 6367 PflMI CCAn_nnn'nTGG Cuts at: 1108 2046 2440 2551 3505 1108 Size: 938 394 111 954 3970 Fragments arranged by size: 3970 954 938 394 111 PfoI T'CCnGG_A Cuts at: 3483 3483 Size: 6367 PmeI GTTT'AAAC Cuts at: 3671 3671 Size: 6367 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4436 4468 5949 5981 4436 Size: 32 1481 32 4822 Fragments arranged by size: 4822 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2914 2914 Size: 6367 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6367 PsiI TTA'TAA Cuts at: 3937 3937 Size: 6367 PspOMI G'GGCC_C Cuts at: 3115 3115 Size: 6367 PstI C_TGCA'G Cuts at: 830 2151 2160 2343 3719 830 Size: 1321 9 183 1376 3478 Fragments arranged by size: 3478 1376 1321 183 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6367 PvuII CAG'CTG Cuts at: 2338 2338 Size: 6367 SalI G'TCGA_C Cuts at: 1936 3709 1936 Size: 1773 4594 SanDI GG'GwC_CC Cuts at: 2914 2914 Size: 6367 SapI GCTCTTCn'nnn_ Cuts at: 2357 2382 2357 Size: 25 6342 SbfI CC_TGCA'GG Cuts at: 3719 3719 Size: 6367 ScaI AGT'ACT Cuts at: 2117 4615 6327 2117 Size: 2498 1712 2157 Fragments arranged by size: 2498 2157 1712 SfcI C'TryA_G Cuts at: 826 1045 2119 2147 2156 2339 3556 3715 4850 Size: 219 1074 28 9 183 1217 159 1135 Cuts at: 4850 5531 5722 826 Size: 681 191 1471 Fragments arranged by size: 1471 1217 1135 1074 681 219 191 183 159 28 9 SfoI GGC'GCC Cuts at: 2020 2020 Size: 6367 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6367 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6367 SmaI CCC'GGG Cuts at: 1872 2872 3694 1872 Size: 1000 822 4545 Fragments arranged by size: 4545 1000 822 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 4471 5372 5634 820 Size: 197 926 306 2222 901 262 1553 Fragments arranged by size: 2222 1553 926 901 306 262 197 SnaBI TAC'GTA Cuts at: 493 4158 493 Size: 3665 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6367 SphI G_CATG'C Cuts at: 1132 3004 3725 1132 Size: 1872 721 3774 Fragments arranged by size: 3774 1872 721 SspI AAT'ATT Cuts at: 5 52 2500 4291 5 Size: 47 2448 1791 2081 Fragments arranged by size: 2448 2081 1791 47 StuI AGG'CCT Cuts at: 1517 2402 3546 1517 Size: 885 1144 4338 Fragments arranged by size: 4338 1144 885 StyI C'CwwG_G Cuts at: 513 1065 2376 2646 2959 3313 3606 3806 513 Size: 552 1311 270 313 354 293 200 3074 Fragments arranged by size: 3074 1311 552 354 313 293 270 200 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2779 4394 4411 4564 4749 5194 325 Size: 1255 1199 1615 17 153 185 445 1498 Fragments arranged by size: 1615 1498 1255 1199 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2115 4613 6325 Size: 274 80 33 51 1443 138 2498 1712 Cuts at: 6325 96 Size: 138 Fragments arranged by size: 2498 1712 1443 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2174 2206 2722 2754 6327 6359 2174 Size: 32 516 32 3573 32 2182 Fragments arranged by size: 3573 2182 516 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6367 XbaI T'CTAG_A Cuts at: 3703 3703 Size: 6367 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2047 2548 2653 3520 2047 Size: 501 105 867 4894 Fragments arranged by size: 4894 867 501 105 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6367 XmaI C'CCGG_G Cuts at: 1870 2870 3692 1870 Size: 1000 822 4545 Fragments arranged by size: 4545 1000 822 XmnI GAAnn'nnTTC Cuts at: 2501 4496 2501 Size: 1995 4372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4175 276 Size: 53 83 186 936 2641 2468 Fragments arranged by size: 2641 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI