Restriction map for
pFN21AB5819
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbsI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MslI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XhoI XhoII
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 782 GTCGAC GT^[AC][GT]AC
Aor51HI 911 AGCGCT AGC^GCT
ApaI 470 GGGCCC GGGCC^C
Asp700I 1143 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1101 TGGCCA TGG^CCA
BbrPI 406 CACGTG CAC^GTG
BbvCI 652 GCTGAGG CCTCAGC(-5/-2)
BcgI 960 GCAGCGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 73 GCCCGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 406 CACGTG [CT]AC^GT[GA]
BsgI 1231 CTGCAC GTGCAG(16/14)
BsmBI 190 GAGACG CGTCTC(1/5)
BspLU11I 449 ACATGT A^CATGT
BsrBI 874 GAGCGG CCGCTC(-3/-3)
BssSI 307 CACGAG CACGAG(-5/-1)
BstAPI 918 GCAGCGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1029 CATCC GGATG(2/0)
DsaI 1118 CCATGG C^C[GA][CT]GG
Eco47III 911 AGCGCT AGC^GCT
FokI 1029 CATCC GGATG(9/13)
HincII 782 GTCGAC GT[CT]^[GA]AC
HindII 782 GTCGAC GT[CT]^[GA]AC
MluNI 1101 TGGCCA TGG^CCA
MscI 1101 TGGCCA TGG^CCA
NcoI 1118 CCATGG C^CATGG
PflMI 1185 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 406 CACGTG CAC^GTG
PmlI 406 CACGTG CAC^GTG
SacI 1091 GAGCTC GAGCT^C
SalI 782 GTCGAC G^TCGAC
ScaI 600 AGTACT AGT^ACT
SmaI 483 CCCGGG CCC^GGG
SmlI 1054 CTCAAG C^T[CT][GA]AG
SphI 923 GCATGC GCATG^C
TaqI 783 TCGA T^CGA
TfiI 613 GAATC G^A[AT]TC
TthHB8I 783 TCGA T^CGA
Van91I 1185 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 483 CCCGGG C^CCGGG
XmaI 483 CCCGGG C^CCGGG
XmnI 1143 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 407 ACGTGT A^C[GA][CT]GT
449 ACATGT
AlwI 42 GGATC GGATC(4/5)
966 GGATC
AlwNI 814 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1186 CAGCTTCTG
AspHI 304 GAGCAC G[AT]GC[AT]^C
1091 GAGCTC
AvaI 483 CCCGGG C^[CT]CG[GA]G
868 CCCGAG
BfaI 61 CTAG C^TAG
373 CTAG
BsaI 107 GAGACC GGTCTC(1/5)
862 GAGACC
BsiHKAI 304 GAGCAC G[AT]GC[AT]^C
1091 GAGCTC
BsoBI 483 CCCGGG C^[CT]CG[GA]G
868 CCCGAG
BsrFI 848 GCCGGC [GA]^CCGG[CT]
953 GCCGGC
Cfr10I 848 GCCGGC [GA]^CCGG[CT]
953 GCCGGC
EcoRI 327 GAATTC G^AATTC
698 GAATTC
EcoT14I 111 CCTTGG C^C[AT][AT]GG
1118 CCATGG
HaeII 436 AGCGCC [GA]GCGC^[CT]
911 AGCGCT
MaeI 61 CTAG C^TAG
373 CTAG
MaeII 407 ACGT A^CGT
780 ACGT
MaeIII 167 GTGAC ^GT[ACGT]AC
313 GTCAC
MseI 508 TTAA T^TAA
578 TTAA
NaeI 848 GCCGGC GCC^GGC
953 GCCGGC
NgoMIV 848 GCCGGC G^CCGGC
953 GCCGGC
NspI 449 ACATGT [GA]CATG^[CT]
923 GCATGC
PleI 154 GACTC GAGTC(4/5)
753 GACTC
PpuMI 639 GGGTCCC [GA]G^G[AT]CC[CT]
1106 AGGACCC
PvuII 1133 CAGCTG CAG^CTG
1153 CAGCTG
StyI 111 CCTTGG C^C[AT][AT]GG
1118 CCATGG
Tru9I 508 TTAA T^TAA
578 TTAA
Tsp45I 167 GTGAC ^GT[GC]AC
313 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PstI RsaI
SapI Sau3AI Sau96I ScrFI SfcI
TseI Tsp509I TspRI