(Circular) (Six-base) MAPSORT of: pFN21AB5819.seq Check: 7995 from: 1 to: 5941 pFN21AB5819 5941 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2788 3284 1937 Size: 851 496 4594 Fragments arranged by size: 4594 851 496 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2622 3011 4004 5388 5656 1632 Size: 990 389 993 1384 268 1917 Fragments arranged by size: 1917 1384 993 990 389 268 AfeI AGC'GCT Cuts at: 2918 2918 Size: 5941 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 2412 2454 4840 5817 1203 Size: 1209 42 2386 977 1327 Fragments arranged by size: 2386 1327 1209 977 42 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2421 3251 1184 Size: 1237 830 3874 Fragments arranged by size: 3874 1237 830 AlwNI CAG_nnn'CTG Cuts at: 2824 3196 3684 5256 2824 Size: 372 488 1572 3509 Fragments arranged by size: 3509 1572 488 372 ApaI G_GGCC'C Cuts at: 2479 2479 Size: 5941 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 2326 2332 2703 3250 3476 3628 5737 1493 Size: 833 6 371 547 226 152 2109 1697 Fragments arranged by size: 2109 1697 833 547 371 226 152 6 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2488 2873 3266 1870 Size: 73 545 385 393 4545 Fragments arranged by size: 4545 545 393 385 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3262 4717 618 Size: 325 217 627 1475 1455 1842 Fragments arranged by size: 1842 1475 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2479 2560 2782 3100 3260 1391 Size: 1088 81 222 318 160 4072 Fragments arranged by size: 4072 1088 318 222 160 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4970 BbvCI CC'TCA_GC Cuts at: 2658 2658 Size: 5941 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2954 2988 4132 4166 1903 Size: 34 1017 34 1144 34 3678 Fragments arranged by size: 3678 1144 1017 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2084 4552 136 Size: 107 122 71 631 605 412 2468 1525 Fragments arranged by size: 2468 1525 631 605 412 122 107 71 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2479 2956 4002 5158 1790 Size: 689 477 1046 1156 2573 Fragments arranged by size: 2573 1156 1046 689 477 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2053 2757 3672 Size: 416 53 83 399 24 636 704 915 Cuts at: 3672 4630 442 Size: 958 1753 Fragments arranged by size: 1753 958 915 704 636 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2665 2665 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2130 2146 2635 2670 3092 4601 1239 Size: 891 16 489 35 422 1509 2579 Fragments arranged by size: 2579 1509 891 489 422 35 16 Bpu10I CC'TnA_GC Cuts at: 2480 2658 3133 2480 Size: 178 475 5288 Fragments arranged by size: 5288 475 178 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3044 4066 4931 5229 3044 Size: 1022 865 298 3756 Fragments arranged by size: 3756 1022 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2106 2861 4604 882 Size: 617 607 755 1743 2219 Fragments arranged by size: 2219 1743 755 617 607 BsaAI yAC'GTr Cuts at: 493 1204 2413 3732 493 Size: 711 1209 1319 2702 Fragments arranged by size: 2702 1319 1209 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3748 4130 4765 275 Size: 53 83 186 936 2215 382 635 1451 Fragments arranged by size: 2215 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4374 4772 5046 5193 5604 4374 Size: 398 274 147 411 4711 Fragments arranged by size: 4711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2232 2249 2262 2279 1178 Size: 30 165 30 829 17 13 17 4840 Fragments arranged by size: 4840 829 165 30 30 17 17 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 2891 3079 1186 Size: 1021 684 188 4048 Fragments arranged by size: 4048 1021 684 188 BseYI C'CCAG_C Cuts at: 2060 5144 5640 2060 Size: 3084 496 2361 Fragments arranged by size: 3084 2361 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3221 1163 Size: 573 1485 3883 Fragments arranged by size: 3883 1485 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2313 3100 4002 4087 5158 2313 Size: 787 902 85 1071 3096 Fragments arranged by size: 3096 1071 902 787 85 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 BsmBI CGTCTCn'nnnn_ Cuts at: 2189 2189 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2313 2479 2560 2782 2956 3100 3260 Size: 399 523 166 81 222 174 144 160 Cuts at: 3260 4002 4087 5158 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 523 399 222 174 166 160 144 85 81 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 844 Size: 2452 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2881 3828 1394 Size: 429 1058 947 3507 Fragments arranged by size: 3507 1058 947 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4436 4610 65 Size: 1183 3188 174 1396 Fragments arranged by size: 3188 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2853 2958 4585 4772 1700 Size: 110 1043 105 1627 187 2869 Fragments arranged by size: 2869 1627 1043 187 110 105 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 2312 4001 5013 2312 Size: 1689 1012 3240 Fragments arranged by size: 3240 1689 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2929 794 Size: 2135 3806 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstYI r'GATC_y Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936 Size: 1299 367 385 17 1452 86 12 346 Cuts at: 5936 1972 Size: 1977 Fragments arranged by size: 1977 1452 1299 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3123 513 Size: 552 2058 3331 Fragments arranged by size: 3331 2058 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2102 487 Size: 683 56 876 4326 Fragments arranged by size: 4326 876 683 56 BtsI GCAGTG_nn' Cuts at: 2176 2424 2439 3457 4251 4271 2176 Size: 248 15 1018 794 20 3846 Fragments arranged by size: 3846 1018 794 248 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2470 2841 3106 Size: 54 1425 50 275 18 640 371 265 Cuts at: 3106 3388 4277 5906 8 Size: 282 889 1629 43 Fragments arranged by size: 1629 1425 889 640 371 282 275 265 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 2184 2221 2843 3210 3871 1353 Size: 711 120 37 622 367 661 3423 Fragments arranged by size: 3423 711 661 622 367 120 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3098 3098 Size: 5941 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 2146 2622 2635 2670 3011 3092 Size: 393 498 16 476 13 35 341 81 Cuts at: 3092 4004 4601 5388 5656 1239 Size: 912 597 787 268 1524 Fragments arranged by size: 1524 912 787 597 498 476 393 341 268 81 35 16 13 EcoO109I rG'GnC_Cy Cuts at: 2476 2645 3112 2476 Size: 169 467 5305 Fragments arranged by size: 5305 467 169 EcoRI G'AATT_C Cuts at: 2332 2703 3250 3628 2332 Size: 371 547 378 4645 Fragments arranged by size: 4645 547 378 371 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3226 3226 Size: 5941 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2445 2920 5088 1678 Size: 767 475 2168 2531 Fragments arranged by size: 2531 2168 767 475 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2121 2144 2153 2176 2198 Size: 32 135 32 549 23 9 23 22 Cuts at: 2198 2230 2232 2264 2519 2551 4582 4614 4656 Size: 32 2 32 255 32 2031 32 42 Cuts at: 4656 4688 1373 Size: 32 2626 Fragments arranged by size: 2626 2031 549 255 135 42 32 32 32 32 32 32 32 23 23 22 9 2 HincII GTy'rAC Cuts at: 669 1657 1938 2789 3285 3531 669 Size: 988 281 851 496 246 3079 Fragments arranged by size: 3079 988 851 496 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1994 2164 3162 5055 5239 1305 Size: 689 170 998 1893 184 2007 Fragments arranged by size: 2007 1893 998 689 184 170 MscI TGG'CCA Cuts at: 10 64 1489 1832 3108 10 Size: 54 1425 343 1276 2843 Fragments arranged by size: 2843 1425 1276 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3899 4258 4417 518 Size: 648 2733 359 159 2042 Fragments arranged by size: 2733 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2821 2950 2968 3140 3160 3357 4034 5182 Size: 1215 129 18 172 20 197 677 1148 Cuts at: 5182 5427 1606 Size: 245 2120 Fragments arranged by size: 2120 1215 1148 677 245 197 172 129 20 18 NaeI GCC'GGC Cuts at: 2855 2960 2855 Size: 105 5836 NcoI C'CATG_G Cuts at: 513 1065 3123 513 Size: 552 2058 3331 Fragments arranged by size: 3331 2058 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NgoMIV G'CCGG_C Cuts at: 2853 2958 2853 Size: 105 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 2458 2932 3299 4844 1132 Size: 97 1229 474 367 1545 2229 Fragments arranged by size: 2229 1545 1229 474 367 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 2454 4840 2454 Size: 2386 3555 PflMI CCAn_nnn'nTGG Cuts at: 1108 3196 1108 Size: 2088 3853 PfoI T'CCnGG_A Cuts at: 2107 2107 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PmlI CAC'GTG Cuts at: 2413 2413 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3215 3247 4010 4042 5523 5555 3215 Size: 32 763 32 1481 32 3601 Fragments arranged by size: 3601 1481 763 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2645 3112 2645 Size: 467 5474 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2475 2475 Size: 5941 PstI C_TGCA'G Cuts at: 830 2431 2820 2967 3293 830 Size: 1601 389 147 326 3478 Fragments arranged by size: 3478 1601 389 326 147 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 3140 3160 3140 Size: 20 5921 SacI G_AGCT'C Cuts at: 3100 3100 Size: 5941 SalI G'TCGA_C Cuts at: 1936 2787 3283 1936 Size: 851 496 4594 Fragments arranged by size: 4594 851 496 SanDI GG'GwC_CC Cuts at: 2645 2645 Size: 5941 SapI GCTCTTCn'nnn_ Cuts at: 2064 2843 3210 2064 Size: 779 367 4795 Fragments arranged by size: 4795 779 367 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 2607 4189 5901 2607 Size: 1582 1712 2647 Fragments arranged by size: 2647 1712 1582 SfcI C'TryA_G Cuts at: 826 1045 2427 2816 2963 3289 4424 5105 5296 Size: 219 1382 389 147 326 1135 681 191 Cuts at: 5296 826 Size: 1471 Fragments arranged by size: 1471 1382 1135 681 389 326 219 191 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2490 3268 1872 Size: 618 778 4545 Fragments arranged by size: 4545 778 618 SmlI C'TyrA_G Cuts at: 820 1017 1943 3059 4045 4946 5208 820 Size: 197 926 1116 986 901 262 1553 Fragments arranged by size: 1553 1116 986 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 2932 3299 1132 Size: 1800 367 3774 Fragments arranged by size: 3774 1800 367 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2116 3123 3380 513 Size: 552 1051 1007 257 3074 Fragments arranged by size: 3074 1051 1007 552 257 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3968 3985 4138 4323 4768 325 Size: 1255 2388 17 153 185 445 1498 Fragments arranged by size: 2388 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2605 4187 5899 Size: 274 80 33 51 1443 628 1582 1712 Cuts at: 5899 96 Size: 138 Fragments arranged by size: 1712 1582 1443 628 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2488 3266 1870 Size: 618 778 4545 Fragments arranged by size: 4545 778 618 XmnI GAAnn'nnTTC Cuts at: 3152 4070 3152 Size: 918 5023 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI