Restriction map for
pFN21AB5819
(Linear) (Six-base) MAPSORT of: KIBB5819.seq Check: 5652 from: 1 to: 1236
KIBB5819.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 0 783 1236
Size: 783 453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1006 1236
Size: 617 389 230
Fragments arranged by size:
617 389 230
AfeI AGC'GCT
Cuts at: 0 913 1236
Size: 913 323
AflIII A'CryG_T
Cuts at: 0 407 449 1236
Size: 407 42 787
Fragments arranged by size:
787 407 42
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 416 1236
Size: 416 820
AlwNI CAG_nnn'CTG
Cuts at: 0 819 1191 1236
Size: 819 372 45
Fragments arranged by size:
819 372 45
ApaI G_GGCC'C
Cuts at: 0 474 1236
Size: 474 762
ApoI r'AATT_y
Cuts at: 0 321 327 698 1236
Size: 321 6 371 538
Fragments arranged by size:
538 371 321 6
AvaI C'yCGr_G
Cuts at: 0 483 868 1236
Size: 483 385 368
Fragments arranged by size:
483 385 368
BanII G_rGCy'C
Cuts at: 0 474 555 777 1095 1236
Size: 474 81 222 318 141
Fragments arranged by size:
474 318 222 141 81
BbvCI CC'TCA_GC
Cuts at: 0 653 1236
Size: 653 583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 949 983 1236
Size: 949 34 253
Fragments arranged by size:
949 253 34
BglI GCCn_nnn'nGGC
Cuts at: 0 79 1236
Size: 79 1157
Bme1580I G_kGCm'C
Cuts at: 0 474 951 1236
Size: 474 477 285
Fragments arranged by size:
477 474 285
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 752 1236
Size: 48 704 484
Fragments arranged by size:
704 484 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 660 1236
Size: 660 576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 141 630 665 1087 1236
Size: 125 16 489 35 422 149
Fragments arranged by size:
489 422 149 125 35 16
Bpu10I CC'TnA_GC
Cuts at: 0 475 653 1128 1236
Size: 475 178 475 108
Fragments arranged by size:
475 475 178 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1236
Size: 1039 197
BsaI GGTCTCn'nnnn_
Cuts at: 0 101 856 1236
Size: 101 755 380
Fragments arranged by size:
755 380 101
BsaAI yAC'GTr
Cuts at: 0 408 1236
Size: 408 828
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 227 244 257 274 1236
Size: 227 17 13 17 962
Fragments arranged by size:
962 227 17 17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 886 1074 1236
Size: 202 684 188 162
Fragments arranged by size:
684 202 188 162
BseYI C'CCAG_C
Cuts at: 0 55 1236
Size: 55 1181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1216 1236
Size: 1216 20
BsiHKAI G_wGCw'C
Cuts at: 0 308 1095 1236
Size: 308 787 141
Fragments arranged by size:
787 308 141
BsmBI CGTCTCn'nnnn_
Cuts at: 0 184 1236
Size: 184 1052
Bsp1286I G_dGCh'C
Cuts at: 0 308 474 555 777 951 1095 1236
Size: 308 166 81 222 174 144 141
Fragments arranged by size:
308 222 174 166 144 141 81
BsrBI CCG'CTC
Cuts at: 0 876 1236
Size: 876 360
BsrFI r'CCGG_y
Cuts at: 0 848 953 1236
Size: 848 105 283
Fragments arranged by size:
848 283 105
BssSI C'ACGA_G
Cuts at: 0 307 1236
Size: 307 929
BstAPI GCAn_nnn'nTGC
Cuts at: 0 924 1236
Size: 924 312
BtgI C'CryG_G
Cuts at: 0 1118 1236
Size: 1118 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 97 1236
Size: 97 1139
BtsI GCAGTG_nn'
Cuts at: 0 171 419 434 1236
Size: 171 248 15 802
Fragments arranged by size:
802 248 171 15
EaeI y'GGCC_r
Cuts at: 0 465 836 1101 1236
Size: 465 371 265 135
Fragments arranged by size:
465 371 265 135
EarI CTCTTCn'nnn_
Cuts at: 0 59 179 216 838 1205 1236
Size: 59 120 37 622 367 31
Fragments arranged by size:
622 367 120 59 37 31
EcoICRI GAG'CTC
Cuts at: 0 1093 1236
Size: 1093 143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 141 617 630 665 1006 1087 1236
Size: 125 16 476 13 35 341 81 149
Fragments arranged by size:
476 341 149 125 81 35 16 13
EcoO109I rG'GnC_Cy
Cuts at: 0 471 640 1107 1236
Size: 471 169 467 129
Fragments arranged by size:
471 467 169 129
EcoRI G'AATT_C
Cuts at: 0 327 698 1236
Size: 327 371 538
Fragments arranged by size:
538 371 327
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1221 1236
Size: 1221 15
HaeII r_GCGC'y
Cuts at: 0 440 915 1236
Size: 440 475 321
Fragments arranged by size:
475 440 321
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 116 139 148 171 193 225 227 259
Size: 116 23 9 23 22 32 2 32
Cuts at: 259 514 546 1236
Size: 255 32 690
Fragments arranged by size:
690 255 116 32 32 32 23 23
22 9 2
HincII GTy'rAC
Cuts at: 0 784 1236
Size: 784 452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 1157 1236
Size: 159 998 79
Fragments arranged by size:
998 159 79
MscI TGG'CCA
Cuts at: 0 1103 1236
Size: 1103 133
MspA1I CmG'CkG
Cuts at: 0 816 945 963 1135 1155 1236
Size: 816 129 18 172 20 81
Fragments arranged by size:
816 172 129 81 20 18
NaeI GCC'GGC
Cuts at: 0 850 955 1236
Size: 850 105 281
Fragments arranged by size:
850 281 105
NcoI C'CATG_G
Cuts at: 0 1118 1236
Size: 1118 118
NgoMIV G'CCGG_C
Cuts at: 0 848 953 1236
Size: 848 105 283
Fragments arranged by size:
848 283 105
NspI r_CATG'y
Cuts at: 0 453 927 1236
Size: 453 474 309
Fragments arranged by size:
474 453 309
PciI A'CATG_T
Cuts at: 0 449 1236
Size: 449 787
PflMI CCAn_nnn'nTGG
Cuts at: 0 1191 1236
Size: 1191 45
PfoI T'CCnGG_A
Cuts at: 0 102 1236
Size: 102 1134
PmlI CAC'GTG
Cuts at: 0 408 1236
Size: 408 828
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1210 1236
Size: 1210 26
PpuMI rG'GwC_Cy
Cuts at: 0 640 1107 1236
Size: 640 467 129
Fragments arranged by size:
640 467 129
PspOMI G'GGCC_C
Cuts at: 0 470 1236
Size: 470 766
PstI C_TGCA'G
Cuts at: 0 426 815 962 1236
Size: 426 389 147 274
Fragments arranged by size:
426 389 274 147
PvuII CAG'CTG
Cuts at: 0 1135 1155 1236
Size: 1135 20 81
Fragments arranged by size:
1135 81 20
SacI G_AGCT'C
Cuts at: 0 1095 1236
Size: 1095 141
SalI G'TCGA_C
Cuts at: 0 782 1236
Size: 782 454
SanDI GG'GwC_CC
Cuts at: 0 640 1236
Size: 640 596
SapI GCTCTTCn'nnn_
Cuts at: 0 59 838 1205 1236
Size: 59 779 367 31
Fragments arranged by size:
779 367 59 31
ScaI AGT'ACT
Cuts at: 0 602 1236
Size: 602 634
SfcI C'TryA_G
Cuts at: 0 422 811 958 1236
Size: 422 389 147 278
Fragments arranged by size:
422 389 278 147
SmaI CCC'GGG
Cuts at: 0 485 1236
Size: 485 751
SmlI C'TyrA_G
Cuts at: 0 1054 1236
Size: 1054 182
SphI G_CATG'C
Cuts at: 0 927 1236
Size: 927 309
StyI C'CwwG_G
Cuts at: 0 111 1118 1236
Size: 111 1007 118
Fragments arranged by size:
1007 118 111
TatI w'GTAC_w
Cuts at: 0 600 1236
Size: 600 636
XmaI C'CCGG_G
Cuts at: 0 483 1236
Size: 483 753
XmnI GAAnn'nnTTC
Cuts at: 0 1147 1236
Size: 1147 89
Enzymes that do cut:
AccI AcuI AfeI AflIII AlfI AlwNI ApaI ApoI
AvaI BanII BbvCI BcgI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BssSI BstAPI
BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII MmeI MscI MspA1I
NaeI NcoI NgoMIV NspI PciI PflMI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuII SacI SalI SanDI
SapI ScaI SfcI SmaI SmlI SphI StyI TatI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrDI BsrGI BssHII BstBI BstEII
BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MslI NarI
NdeI NheI NotI NruI NsiI PacI PasI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI ZraI