(Circular) (Six-base) MAPSORT of: pFN21AB5807.seq Check: 3130 from: 1 to: 6121 pFN21AB5807 6121 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2021 2021 Size: 6121 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3931 278 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3464 1937 Size: 1527 4594 Acc65I G'GTAC_C Cuts at: 2454 3442 2454 Size: 988 5133 AclI AA'CG_TT Cuts at: 1563 3420 4248 4621 1563 Size: 1857 828 373 3063 Fragments arranged by size: 3063 1857 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2206 2750 2771 4184 5568 5836 1632 Size: 574 544 21 1413 1384 268 1917 Fragments arranged by size: 1917 1413 1384 574 544 268 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 5020 5997 1203 Size: 3817 977 1327 Fragments arranged by size: 3817 1327 977 AgeI A'CCGG_T Cuts at: 4952 4952 Size: 6121 AhdI GACnn_n'nnGTC Cuts at: 3093 4850 3093 Size: 1757 4364 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2487 1184 Size: 1303 4818 AlwNI CAG_nnn'CTG Cuts at: 3025 3864 5436 3025 Size: 839 1572 3710 Fragments arranged by size: 3710 1572 839 ApaLI G'TGCA_C Cuts at: 3368 4178 5334 3368 Size: 810 1156 4155 Fragments arranged by size: 4155 1156 810 ApoI r'AATT_y Cuts at: 1493 3430 3656 3808 5917 1493 Size: 1937 226 152 2109 1697 Fragments arranged by size: 2109 1937 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4675 160 Size: 872 3643 1606 Fragments arranged by size: 3643 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 3446 1870 Size: 73 1503 4545 Fragments arranged by size: 4545 1503 73 BamHI G'GATC_C Cuts at: 3451 3818 3451 Size: 367 5754 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2454 3442 4897 618 Size: 325 217 627 667 988 1455 1842 Fragments arranged by size: 1842 1455 988 667 627 325 217 BanII G_rGCy'C Cuts at: 1391 2054 3006 3440 1391 Size: 663 952 434 4072 Fragments arranged by size: 4072 952 663 434 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3224 928 Size: 971 1325 3825 Fragments arranged by size: 3825 1325 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3389 3423 4312 4346 1903 Size: 34 1452 34 889 34 3678 Fragments arranged by size: 3678 1452 889 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4013 5223 4013 Size: 1210 4911 BclI T'GATC_A Cuts at: 1548 1548 Size: 6121 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2588 4732 136 Size: 107 122 71 631 605 916 2144 1525 Fragments arranged by size: 2144 1525 916 631 605 122 107 71 BglII A'GATC_T Cuts at: 2994 6116 2994 Size: 3122 2999 BlpI GC'TnA_GC Cuts at: 3050 3538 3050 Size: 488 5633 Bme1580I G_kGCm'C Cuts at: 1790 3372 4182 5338 1790 Size: 1582 810 1156 2573 Fragments arranged by size: 2573 1582 1156 810 BmgBI CAC'GTC Cuts at: 1318 3214 1318 Size: 1896 4225 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3286 3852 4810 Size: 416 53 83 399 24 1869 566 958 Cuts at: 4810 442 Size: 1753 Fragments arranged by size: 1869 1753 958 566 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2020 2071 2432 2729 3047 3089 3101 3248 Size: 781 51 361 297 318 42 12 147 Cuts at: 3248 3281 3319 4781 1239 Size: 33 38 1462 2579 Fragments arranged by size: 2579 1462 781 361 318 297 147 51 42 38 33 12 Bpu10I CC'TnA_GC Cuts at: 2843 2843 Size: 6121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4246 5111 5409 4246 Size: 865 298 4958 Fragments arranged by size: 4958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3080 4784 882 Size: 617 1581 1704 2219 Fragments arranged by size: 2219 1704 1581 617 BsaAI yAC'GTr Cuts at: 493 1204 3912 493 Size: 711 2708 2702 Fragments arranged by size: 2708 2702 711 BsaBI GATnn'nnATC Cuts at: 6115 6115 Size: 6121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3273 3928 4310 4945 Size: 53 83 186 936 1740 655 382 635 Cuts at: 4945 275 Size: 1451 Fragments arranged by size: 1740 1451 936 655 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4554 4952 5226 5373 5784 4554 Size: 398 274 147 411 4891 Fragments arranged by size: 4891 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3269 3299 1178 Size: 30 165 30 1866 30 4000 Fragments arranged by size: 4000 1866 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2089 2167 2429 2750 2780 2783 2951 Size: 837 66 78 262 321 30 3 168 Cuts at: 2951 3098 3161 3274 3277 3289 3322 1186 Size: 147 63 113 3 12 33 3985 Fragments arranged by size: 3985 837 321 262 168 147 113 78 66 63 33 30 12 3 3 BseYI C'CCAG_C Cuts at: 2132 3045 3159 5324 5820 2132 Size: 913 114 2165 496 2433 Fragments arranged by size: 2433 2165 913 496 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2811 2996 1163 Size: 573 1075 185 4288 Fragments arranged by size: 4288 1075 573 185 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2377 3571 4332 5360 1540 Size: 275 186 376 1194 761 1028 2301 Fragments arranged by size: 2301 1194 1028 761 376 275 186 BsiHKAI G_wGCw'C Cuts at: 2410 3006 3372 4182 4267 5338 2410 Size: 596 366 810 85 1071 3193 Fragments arranged by size: 3193 1071 810 596 366 85 BsmI GAATG_Cn' Cuts at: 2782 2850 3632 3725 2782 Size: 68 782 93 5178 Fragments arranged by size: 5178 782 93 68 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2410 3006 3372 3440 4182 4267 Size: 399 264 356 596 366 68 742 85 Cuts at: 4267 5338 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 596 399 366 356 264 85 68 BspEI T'CCGG_A Cuts at: 5784 5784 Size: 6121 BspHI T'CATG_A Cuts at: 4010 5740 4010 Size: 1730 4391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2021 2640 3391 3476 844 Size: 1177 619 751 85 3489 Fragments arranged by size: 3489 1177 751 619 85 BsrBI CCG'CTC Cuts at: 1394 1823 2982 4008 1394 Size: 429 1159 1026 3507 Fragments arranged by size: 3507 1159 1026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4616 4790 65 Size: 1183 3368 174 1396 Fragments arranged by size: 3368 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4765 4952 1700 Size: 110 2955 187 2869 Fragments arranged by size: 2955 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6121 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6121 BssSI C'ACGA_G Cuts at: 2762 3395 4181 5193 2762 Size: 633 786 1012 3690 Fragments arranged by size: 3690 1012 786 633 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6121 BstBI TT'CG_AA Cuts at: 4925 4925 Size: 6121 BstEII G'GTnAC_C Cuts at: 1184 2353 1184 Size: 1169 4952 BstXI CCAn_nnnn'nTGG Cuts at: 2798 3361 2798 Size: 563 5558 BstYI r'GATC_y Cuts at: 1972 2442 2994 3120 3201 3451 3818 4203 4220 Size: 470 552 126 81 250 367 385 17 Cuts at: 4220 5672 5758 5770 6116 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 552 470 385 367 346 250 126 86 81 17 12 Bsu36I CC'TnA_GG Cuts at: 2519 2519 Size: 6121 BtgI C'CryG_G Cuts at: 513 1065 2592 513 Size: 552 1527 4042 Fragments arranged by size: 4042 1527 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5382 Fragments arranged by size: 5382 683 56 BtsI GCAGTG_nn' Cuts at: 2350 3637 4431 4451 2350 Size: 1287 794 20 4020 Fragments arranged by size: 4020 1287 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6121 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6086 DraI TTT'AAA Cuts at: 3425 3425 Size: 6121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5128 809 Size: 507 3812 1802 Fragments arranged by size: 3812 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2589 2694 Size: 54 1425 50 275 18 279 480 105 Cuts at: 2694 2955 3568 4457 6086 8 Size: 261 613 889 1629 43 Fragments arranged by size: 1629 1425 889 613 480 279 275 261 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3568 1537 Size: 275 1756 4090 Fragments arranged by size: 4090 1756 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4051 1353 Size: 2698 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4708 5082 5228 387 Size: 1064 138 3119 374 146 1280 Fragments arranged by size: 3119 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3004 3004 Size: 6121 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2071 2206 2432 2729 2750 2771 Size: 393 388 51 135 226 297 21 21 Cuts at: 2771 3047 3089 3101 3248 3281 3319 4184 4781 Size: 276 42 12 147 33 38 865 597 Cuts at: 4781 5568 5836 1239 Size: 787 268 1524 Fragments arranged by size: 1524 865 787 597 393 388 297 276 268 226 147 135 51 42 38 33 21 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 2396 2732 3260 2396 Size: 336 528 5257 Fragments arranged by size: 5257 528 336 EcoO109I rG'GnC_Cy Cuts at: 2118 2515 3403 2118 Size: 397 888 4836 Fragments arranged by size: 4836 888 397 EcoRI G'AATT_C Cuts at: 3430 3808 3430 Size: 378 5743 FspI TGC'GCA Cuts at: 2636 2669 4627 2636 Size: 33 1958 4130 Fragments arranged by size: 4130 1958 33 HaeII r_GCGC'y Cuts at: 1678 5268 1678 Size: 3590 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3026 3058 3079 3111 3314 Size: 32 135 32 1454 32 21 32 203 Cuts at: 3314 3346 4762 4794 4836 4868 1373 Size: 32 1416 32 42 32 2626 Fragments arranged by size: 2626 1454 1416 203 135 42 32 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 3465 3711 669 Size: 988 281 1527 246 3079 Fragments arranged by size: 3079 1527 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6121 HpaI GTT'AAC Cuts at: 3711 3711 Size: 6121 KpnI G_GTAC'C Cuts at: 2458 3446 2458 Size: 988 5133 MfeI C'AATT_G Cuts at: 3720 3720 Size: 6121 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5235 5419 1305 Size: 3930 184 2007 Fragments arranged by size: 3930 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2696 2957 10 Size: 54 1425 343 864 261 3174 Fragments arranged by size: 3174 1425 864 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2642 3365 4079 4438 4597 518 Size: 648 1476 723 714 359 159 2042 Fragments arranged by size: 2042 1476 723 714 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2467 2594 3112 3154 3168 3537 4214 5362 Size: 861 127 518 42 14 369 677 1148 Cuts at: 5362 5607 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 861 677 518 369 245 127 42 14 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5569 NdeI CA'TA_TG Cuts at: 387 387 Size: 6121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 3568 3568 Size: 6121 NspI r_CATG'y Cuts at: 1132 1229 3109 3479 5024 1132 Size: 97 1880 370 1545 2229 Fragments arranged by size: 2229 1880 1545 370 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6121 PciI A'CATG_T Cuts at: 5020 5020 Size: 6121 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6121 PfoI T'CCnGG_A Cuts at: 3123 3123 Size: 6121 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2903 2935 4190 4222 5703 5735 2903 Size: 32 1255 32 1481 32 3289 Fragments arranged by size: 3289 1481 1255 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2118 2515 3403 2118 Size: 397 888 4836 Fragments arranged by size: 4836 888 397 PshAI GACnn'nnGTC Cuts at: 1613 2490 1613 Size: 877 5244 PsiI TTA'TAA Cuts at: 3691 3691 Size: 6121 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3179 3211 3179 Size: 32 6089 PstI C_TGCA'G Cuts at: 830 2018 2571 3042 3473 830 Size: 1188 553 471 431 3478 Fragments arranged by size: 3478 1188 553 471 431 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6121 PvuII CAG'CTG Cuts at: 3112 3154 3112 Size: 42 6079 SacI G_AGCT'C Cuts at: 3006 3006 Size: 6121 SacII CC_GC'GG Cuts at: 2595 2595 Size: 6121 SalI G'TCGA_C Cuts at: 1936 3463 1936 Size: 1527 4594 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SfcI C'TryA_G Cuts at: 826 1045 2014 2488 2567 3038 3469 4604 5285 Size: 219 969 474 79 471 431 1135 681 Cuts at: 5285 5476 826 Size: 191 1471 Fragments arranged by size: 1471 1135 969 681 474 471 431 219 191 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 3448 1872 Size: 1576 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4225 5126 5388 820 Size: 197 926 2282 901 262 1553 Fragments arranged by size: 2282 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3912 493 Size: 3419 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6121 SphI G_CATG'C Cuts at: 1132 3109 3479 1132 Size: 1977 370 3774 Fragments arranged by size: 3774 1977 370 SspI AAT'ATT Cuts at: 5 52 4045 5 Size: 47 3993 2081 Fragments arranged by size: 3993 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6121 StyI C'CwwG_G Cuts at: 513 1065 2278 3354 3560 513 Size: 552 1213 1076 206 3074 Fragments arranged by size: 3074 1213 1076 552 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2282 2786 3407 4148 4165 4318 4503 Size: 1255 702 504 621 741 17 153 185 Cuts at: 4503 4948 325 Size: 445 1498 Fragments arranged by size: 1498 1255 741 702 621 504 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3195 4367 6079 Size: 274 80 33 51 1443 1218 1172 1712 Cuts at: 6079 96 Size: 138 Fragments arranged by size: 1712 1443 1218 1172 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3269 3301 6081 6113 3269 Size: 32 2780 32 3277 Fragments arranged by size: 3277 2780 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6121 XbaI T'CTAG_A Cuts at: 3457 3457 Size: 6121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2428 2704 2968 3022 3131 3299 2428 Size: 276 264 54 109 168 5250 Fragments arranged by size: 5250 276 264 168 109 54 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 3446 1870 Size: 1576 4545 XmnI GAAnn'nnTTC Cuts at: 4250 4250 Size: 6121 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3929 276 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI