(Circular) (Six-base) MAPSORT of: pFN21AB5801.seq Check: 4621 from: 1 to: 5818 pFN21AB5801 5818 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3628 278 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3161 1937 Size: 1224 4594 Acc65I G'GTAC_C Cuts at: 3139 3139 Size: 5818 AclI AA'CG_TT Cuts at: 1563 3945 4318 1563 Size: 2382 373 3063 Fragments arranged by size: 3063 2382 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3881 5265 5533 1632 Size: 2249 1384 268 1917 Fragments arranged by size: 2249 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5621 AflIII A'CryG_T Cuts at: 1203 2619 4717 5694 1203 Size: 1416 2098 977 1327 Fragments arranged by size: 2098 1416 1327 977 AgeI A'CCGG_T Cuts at: 4649 4649 Size: 5818 AhdI GACnn_n'nnGTC Cuts at: 4547 4547 Size: 5818 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5818 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5818 AlwNI CAG_nnn'CTG Cuts at: 2997 3561 5133 2997 Size: 564 1572 3682 Fragments arranged by size: 3682 1572 564 ApaLI G'TGCA_C Cuts at: 3875 5031 3875 Size: 1156 4662 ApoI r'AATT_y Cuts at: 1493 2060 2293 2341 3101 3127 3353 3505 5614 Size: 567 233 48 760 26 226 152 2109 Cuts at: 5614 1493 Size: 1697 Fragments arranged by size: 2109 1697 760 567 233 226 152 48 26 AseI AT'TA_AT Cuts at: 160 1032 4372 160 Size: 872 3340 1606 Fragments arranged by size: 3340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 AvaI C'yCGr_G Cuts at: 1870 1943 2497 2567 2775 3143 1870 Size: 73 554 70 208 368 4545 Fragments arranged by size: 4545 554 368 208 73 70 BamHI G'GATC_C Cuts at: 2562 2947 3148 3515 2562 Size: 385 201 367 4865 Fragments arranged by size: 4865 385 367 201 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3139 4594 618 Size: 325 217 627 1352 1455 1842 Fragments arranged by size: 1842 1455 1352 627 325 217 BanII G_rGCy'C Cuts at: 1391 2536 2807 3137 1391 Size: 1145 271 330 4072 Fragments arranged by size: 4072 1145 330 271 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2446 2645 2837 928 Size: 971 547 199 192 3909 Fragments arranged by size: 3909 971 547 199 192 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2147 2181 2702 2736 2964 2998 4009 Size: 34 210 34 521 34 228 34 1011 Cuts at: 4009 4043 1903 Size: 34 3678 Fragments arranged by size: 3678 1011 521 228 210 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 4920 3710 Size: 1210 4608 BclI T'GATC_A Cuts at: 1548 1548 Size: 5818 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4429 136 Size: 107 122 71 631 605 2757 1525 Fragments arranged by size: 2757 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2032 5813 2032 Size: 3781 2037 BlpI GC'TnA_GC Cuts at: 3235 3235 Size: 5818 Bme1580I G_kGCm'C Cuts at: 1790 3879 5035 1790 Size: 2089 1156 2573 Fragments arranged by size: 2573 2089 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5818 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2720 2810 3036 Size: 416 53 83 399 24 1303 90 226 Cuts at: 3036 3549 4507 442 Size: 513 958 1753 Fragments arranged by size: 1753 1303 958 513 416 399 226 90 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5818 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2674 4478 1239 Size: 1435 1804 2579 Fragments arranged by size: 2579 1804 1435 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3943 4808 5106 3943 Size: 865 298 4655 Fragments arranged by size: 4655 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2470 4481 882 Size: 617 971 2011 2219 Fragments arranged by size: 2219 2011 971 617 BsaAI yAC'GTr Cuts at: 493 1204 3609 493 Size: 711 2405 2702 Fragments arranged by size: 2702 2405 711 BsaBI GATnn'nnATC Cuts at: 5812 5812 Size: 5818 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3625 4007 4642 275 Size: 53 83 186 936 2092 382 635 1451 Fragments arranged by size: 2092 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4251 4649 4923 5070 5481 4251 Size: 398 274 147 411 4588 Fragments arranged by size: 4588 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2433 2463 2521 2551 1178 Size: 30 165 30 1030 30 58 30 4445 Fragments arranged by size: 4445 1030 165 58 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2609 2612 2615 2759 2762 1186 Size: 1423 3 3 144 3 4242 Fragments arranged by size: 4242 1423 144 3 3 3 BseYI C'CCAG_C Cuts at: 2871 5021 5517 2871 Size: 2150 496 3172 Fragments arranged by size: 3172 2150 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2215 1163 Size: 573 479 4766 Fragments arranged by size: 4766 573 479 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3268 4029 5057 1540 Size: 275 186 1267 761 1028 2301 Fragments arranged by size: 2301 1267 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2536 3879 3964 5035 2536 Size: 1343 85 1071 3319 Fragments arranged by size: 3319 1343 1071 85 BsmI GAATG_Cn' Cuts at: 3329 3422 3329 Size: 93 5725 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2536 2807 3137 3879 3964 5035 1391 Size: 399 746 271 330 742 85 1071 2174 Fragments arranged by size: 2174 1071 746 742 399 330 271 85 BspEI T'CCGG_A Cuts at: 5481 5481 Size: 5818 BspHI T'CATG_A Cuts at: 3707 5437 3707 Size: 1730 4088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2679 3173 844 Size: 1835 494 3489 Fragments arranged by size: 3489 1835 494 BsrBI CCG'CTC Cuts at: 1394 1823 3705 1394 Size: 429 1882 3507 Fragments arranged by size: 3507 1882 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2391 2959 4313 4487 65 Size: 1183 1143 568 1354 174 1396 Fragments arranged by size: 1396 1354 1183 1143 568 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3000 4462 4649 1700 Size: 110 1190 1462 187 2869 Fragments arranged by size: 2869 1462 1190 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5818 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5818 BssSI C'ACGA_G Cuts at: 2149 3878 4890 2149 Size: 1729 1012 3077 Fragments arranged by size: 3077 1729 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2193 2390 2997 794 Size: 1399 197 607 3615 Fragments arranged by size: 3615 1399 607 197 BstBI TT'CG_AA Cuts at: 2908 4622 2908 Size: 1714 4104 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5818 BstXI CCAn_nnnn'nTGG Cuts at: 2871 2894 2871 Size: 23 5795 BstYI r'GATC_y Cuts at: 1972 2032 2562 2947 3148 3515 3900 3917 5369 Size: 60 530 385 201 367 385 17 1452 Cuts at: 5369 5455 5467 5813 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 530 385 385 367 346 201 86 60 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2085 2434 2589 2643 513 Size: 552 939 81 349 155 54 3688 Fragments arranged by size: 3688 939 552 349 155 81 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2237 487 Size: 683 56 1011 4068 Fragments arranged by size: 4068 1011 683 56 BtsI GCAGTG_nn' Cuts at: 3334 4128 4148 3334 Size: 794 20 5004 Fragments arranged by size: 5004 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5818 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5783 DraI TTT'AAA Cuts at: 2364 3122 2364 Size: 758 5060 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4825 809 Size: 507 3509 1802 Fragments arranged by size: 3509 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3265 4154 5783 Size: 54 1425 50 275 18 1435 889 1629 Cuts at: 5783 8 Size: 43 Fragments arranged by size: 1629 1435 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3265 1537 Size: 275 1453 4090 Fragments arranged by size: 4090 1453 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2601 2919 3748 1353 Size: 1248 318 829 3423 Fragments arranged by size: 3423 1248 829 318 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4405 4779 4925 387 Size: 1064 138 2816 374 146 1280 Fragments arranged by size: 2816 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2534 2534 Size: 5818 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2674 3881 4478 5265 5533 1239 Size: 393 1042 1207 597 787 268 1524 Fragments arranged by size: 1524 1207 1042 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2973 3095 2973 Size: 122 5696 EcoO109I rG'GnC_Cy Cuts at: 2181 2556 2571 3031 2181 Size: 375 15 460 4968 Fragments arranged by size: 4968 460 375 15 EcoRI G'AATT_C Cuts at: 2293 3127 3505 2293 Size: 834 378 4606 Fragments arranged by size: 4606 834 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2068 2405 2068 Size: 337 5481 FspI TGC'GCA Cuts at: 4324 4324 Size: 5818 HaeII r_GCGC'y Cuts at: 1678 4965 1678 Size: 3287 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2297 2329 2433 2453 2465 Size: 32 135 32 725 32 104 20 12 Cuts at: 2465 2485 2521 2553 4459 4491 4533 4565 1373 Size: 20 36 32 1906 32 42 32 2626 Fragments arranged by size: 2626 1906 725 135 104 42 36 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 3162 3408 669 Size: 988 281 1224 246 3079 Fragments arranged by size: 3079 1224 988 281 246 HindIII A'AGCT_T Cuts at: 748 2416 748 Size: 1668 4150 HpaI GTT'AAC Cuts at: 3408 3408 Size: 5818 KpnI G_GTAC'C Cuts at: 3143 3143 Size: 5818 MfeI C'AATT_G Cuts at: 3417 3417 Size: 5818 MluI A'CGCG_T Cuts at: 5694 5694 Size: 5818 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2334 2484 2733 4932 5116 1305 Size: 1029 150 249 2199 184 2007 Fragments arranged by size: 2199 2007 1029 249 184 150 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3996 Fragments arranged by size: 3996 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2624 3776 4135 4294 518 Size: 648 1458 1152 359 159 2042 Fragments arranged by size: 2042 1458 1152 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3234 3911 5059 5304 1606 Size: 1628 677 1148 245 2120 Fragments arranged by size: 2120 1628 1148 677 245 NaeI GCC'GGC Cuts at: 3002 3002 Size: 5818 NcoI C'CATG_G Cuts at: 513 1065 2004 2085 2589 513 Size: 552 939 81 504 3742 Fragments arranged by size: 3742 939 552 504 81 NdeI CA'TA_TG Cuts at: 387 387 Size: 5818 NgoMIV G'CCGG_C Cuts at: 3000 3000 Size: 5818 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5818 NotI GC'GGCC_GC Cuts at: 3265 3265 Size: 5818 NspI r_CATG'y Cuts at: 1132 1229 2623 3077 3176 4721 1132 Size: 97 1394 454 99 1545 2229 Fragments arranged by size: 2229 1545 1394 454 99 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5818 PciI A'CATG_T Cuts at: 2619 4717 2619 Size: 2098 3720 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5818 PfoI T'CCnGG_A Cuts at: 2510 2558 2510 Size: 48 5770 PmeI GTTT'AAAC Cuts at: 3122 3122 Size: 5818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3887 3919 5400 5432 3887 Size: 32 1481 32 4273 Fragments arranged by size: 4273 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2181 2556 2571 3031 2181 Size: 375 15 460 4968 Fragments arranged by size: 4968 460 375 15 PshAI GACnn'nnGTC Cuts at: 1613 3029 1613 Size: 1416 4402 PsiI TTA'TAA Cuts at: 3388 3388 Size: 5818 PstI C_TGCA'G Cuts at: 830 2160 2768 2858 2993 3170 830 Size: 1330 608 90 135 177 3478 Fragments arranged by size: 3478 1330 608 177 135 90 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5818 SacI G_AGCT'C Cuts at: 2536 2536 Size: 5818 SalI G'TCGA_C Cuts at: 1936 3160 1936 Size: 1224 4594 SanDI GG'GwC_CC Cuts at: 2571 2571 Size: 5818 SbfI CC_TGCA'GG Cuts at: 3170 3170 Size: 5818 ScaI AGT'ACT Cuts at: 4066 5778 4066 Size: 1712 4106 SexAI A'CCwGG_T Cuts at: 2546 2546 Size: 5818 SfcI C'TryA_G Cuts at: 826 1045 2156 2176 2764 2854 2989 3166 4301 Size: 219 1111 20 588 90 135 177 1135 Cuts at: 4301 4982 5173 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1111 681 588 219 191 177 135 90 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5818 SmaI CCC'GGG Cuts at: 1872 2499 3145 1872 Size: 627 646 4545 Fragments arranged by size: 4545 646 627 SmlI C'TyrA_G Cuts at: 820 1017 1943 2775 3922 4823 5085 820 Size: 197 926 832 1147 901 262 1553 Fragments arranged by size: 1553 1147 926 901 832 262 197 SnaBI TAC'GTA Cuts at: 493 3609 493 Size: 3116 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5818 SphI G_CATG'C Cuts at: 1132 3077 3176 1132 Size: 1945 99 3774 Fragments arranged by size: 3774 1945 99 SspI AAT'ATT Cuts at: 5 52 3742 5 Size: 47 3690 2081 Fragments arranged by size: 3690 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5818 StyI C'CwwG_G Cuts at: 513 1065 2004 2085 2589 3257 513 Size: 552 939 81 504 668 3074 Fragments arranged by size: 3074 939 668 552 504 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3845 3862 4015 4200 4645 325 Size: 1255 2265 17 153 185 445 1498 Fragments arranged by size: 2265 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2616 4064 5776 Size: 274 80 33 51 1443 639 1448 1712 Cuts at: 5776 96 Size: 138 Fragments arranged by size: 1712 1448 1443 639 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5778 5810 5778 Size: 32 5786 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5818 XbaI T'CTAG_A Cuts at: 3154 3154 Size: 5818 XhoI C'TCGA_G Cuts at: 1943 2775 1943 Size: 832 4986 XmaI C'CCGG_G Cuts at: 1870 2497 3143 1870 Size: 627 646 4545 Fragments arranged by size: 4545 646 627 XmnI GAAnn'nnTTC Cuts at: 3947 3947 Size: 5818 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3626 276 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI