Restriction map for
pFN21AB9914
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# NO CUT ENZYMES ( 109 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BsaBI BsaWI BseAI
BsgI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoT22I
FseI FspI HaeII HindIII HpaI
KasI KpnI KspI MamI MfeI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AocI 702 CCTCAGG CC^T[ACGT]AGG
Asp700I 1300 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 4 GGATCC G^GATCC
BanI 1314 GGCACC G^G[CT][GA]CC
BanII 619 GAGCCC G[GA]GC[CT]^C
BbsI 1515 GAAGAC GAAGAC(2/6)
BclI 1067 TGATCA T^GATCA
BcnI 889 CCCGG CC^[GC]GG
BfaI 370 CTAG C^TAG
BlpI 633 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 633 GCTGAGC GC^T[ACGT]AGC
BpuAI 1515 GAAGAC GAAGAC(2/6)
BsaAI 903 CACGTA [CT]AC^GT[GA]
BsiEI 1284 CGACCG CG[GA][CT]^CG
BsmFI 677 GGGAC GGGAC(10/14)
Bsp1407I 791 TGTACA T^GTACA
BspDI 49 ATCGAT AT^CGAT
BspLU11I 650 ACATGT A^CATGT
BsrGI 791 TGTACA T^GTACA
Bsu36I 702 CCTCAGG CC^T[ACGT]AGG
CelII 633 GCTGAGC GC^T[ACGT]AGC
ClaI 49 ATCGAT AT^CGAT
CpoI 935 CGGTCCG CG^G[AT]CCG
DraII 581 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 172 GACGCCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1411 CTCTTC CTCTTC(1/4)
Eco81I 702 CCTCAGG CC^T[ACGT]AGG
EcoO109I 581 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 207 GAATTC G^AATTC
FbaI 1067 TGATCA T^GATCA
HincII 519 GTTGAC GT[CT]^[GA]AC
HindII 519 GTTGAC GT[CT]^[GA]AC
Ksp632I 1411 CTCTTC CTCTTC(1/4)
MaeI 370 CTAG C^TAG
MluI 378 ACGCGT A^CGCGT
MslI 1086 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 889 CCCGG CC^[GC]GG
PleI 522 GACTC GAGTC(4/5)
RsrII 935 CGGTCCG CG^G[AT]CCG
SapI 1410 GCTCTTC GCTCTTC(1/4)
ScaI 83 AGTACT AGT^ACT
SmlI 312 CTCAAG C^T[CT][GA]AG
SspBI 791 TGTACA T^GTACA
SspI 1268 AATATT AAT^ATT
StuI 1302 AGGCCT AGG^CCT
XcmI 95 CCAGCGACATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1300 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 604 GACGTC GACGT^C
1530 GACGTC
AcsI 207 GAATTC [GA]^AATT[CT]
1140 GAATTT
ApoI 207 GAATTC [GA]^AATT[CT]
1140 GAATTT
AseI 273 ATTAAT AT^TAAT
1074 ATTAAT
AsnI 273 ATTAAT AT^TAAT
1074 ATTAAT
AspHI 646 GAGCAC G[AT]GC[AT]^C
1162 GAGCAC
AvaI 664 CTCGGG C^[CT]CG[GA]G
1457 CCCGAG
BalI 242 TGGCCA TGG^CCA
1082 TGGCCA
BsiHKAI 646 GAGCAC G[AT]GC[AT]^C
1162 GAGCAC
BsmI 59 GCATTC GAATGC(1/-1)
319 GCATTC
BsoBI 664 CTCGGG C^[CT]CG[GA]G
1457 CCCGAG
BspMI 557 ACCTGC ACCTGC(4/8)
294 GCAGGT
BsrFI 23 ACCGGC [GA]^CCGG[CT]
127 ACCGGC
BstAPI 80 GCAAGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1096 GCAAGACATGC
BstF5I 886 CATCC GGATG(2/0)
1029 CATCC
BstXI 35 CCATCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1085 CCACTGACATGG
CfoI 292 GCGC GCG^C
977 GCGC
Cfr10I 23 ACCGGC [GA]^CCGG[CT]
127 ACCGGC
EcoRV 841 GATATC GAT^ATC
1204 GATATC
FauI 202 CCCGC CCCGC(4/6)
920 CCCGC
FokI 886 CATCC GGATG(9/13)
1029 CATCC
HhaI 292 GCGC GCG^C
977 GCGC
HinP1I 292 GCGC G^CGC
977 GCGC
HphI 698 TCACC GGTGA(8/7)
955 TCACC
MluNI 242 TGGCCA TGG^CCA
1082 TGGCCA
MscI 242 TGGCCA TGG^CCA
1082 TGGCCA
MspA1I 860 CAGCGG C[AC]G^C[GT]G
958 CCGCTG
NcoI 176 CCATGG C^CATGG
1418 CCATGG
NspI 650 ACATGT [GA]CATG^[CT]
1101 ACATGC
PflMI 873 CCACGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1033 CCACCTGATGG
PshBI 273 ATTAAT AT^TAAT
1074 ATTAAT
PstI 810 CTGCAG CTGCA^G
1438 CTGCAG
TfiI 1117 GATTC G^A[AT]TC
1453 GAATC
Van91I 873 CCACGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1033 CCACCTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AvaII BbvI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BssKI
BstNI BstUI BstYI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I Fnu4HI HaeIII HapII HgaI
Hin1I HinfI HpaII ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII