(Circular) (Six-base) MAPSORT of: pFN21AB9914.seq Check: 2944 from: 1 to: 6304 pFN21AB9914 6304 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2613 3539 4114 278 Size: 53 83 186 936 1077 926 575 2468 Fragments arranged by size: 2468 1077 936 926 575 186 83 53 AccI GT'mk_AC Cuts at: 1937 3647 1937 Size: 1710 4594 Acc65I G'GTAC_C Cuts at: 3625 3625 Size: 6304 AclI AA'CG_TT Cuts at: 1563 4431 4804 1563 Size: 2868 373 3063 Fragments arranged by size: 3063 2868 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2065 2370 2671 3203 3503 4367 5751 6019 Size: 433 305 301 532 300 864 1384 268 Cuts at: 6019 1632 Size: 1917 Fragments arranged by size: 1917 1384 864 532 433 305 301 300 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6107 AflIII A'CryG_T Cuts at: 1203 2383 2655 2907 5203 6180 1203 Size: 1180 272 252 2296 977 1327 Fragments arranged by size: 2296 1327 1180 977 272 252 AgeI A'CCGG_T Cuts at: 5135 5135 Size: 6304 AhdI GACnn_n'nnGTC Cuts at: 5033 5033 Size: 6304 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6304 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6304 AlwNI CAG_nnn'CTG Cuts at: 4047 5619 4047 Size: 1572 4732 ApaLI G'TGCA_C Cuts at: 4361 5517 4361 Size: 1156 5148 ApoI r'AATT_y Cuts at: 1493 2212 3145 3613 3839 3991 6100 1493 Size: 719 933 468 226 152 2109 1697 Fragments arranged by size: 2109 1697 933 719 468 226 152 AseI AT'TA_AT Cuts at: 160 1032 2279 3080 4858 160 Size: 872 1247 801 1778 1606 Fragments arranged by size: 1778 1606 1247 872 801 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 AvaI C'yCGr_G Cuts at: 1870 1943 2669 3462 3629 1870 Size: 73 726 793 167 4545 Fragments arranged by size: 4545 793 726 167 73 BamHI G'GATC_C Cuts at: 2009 3634 4001 2009 Size: 1625 367 4312 Fragments arranged by size: 4312 1625 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3319 3625 5080 618 Size: 325 217 627 1532 306 1455 1842 Fragments arranged by size: 1842 1532 1455 627 325 306 217 BanII G_rGCy'C Cuts at: 1391 2628 3623 1391 Size: 1237 995 4072 Fragments arranged by size: 4072 1237 995 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3527 928 Size: 971 1628 3705 Fragments arranged by size: 3705 1628 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4495 4529 1903 Size: 34 2558 34 3678 Fragments arranged by size: 3678 2558 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4196 5406 4196 Size: 1210 5094 BclI T'GATC_A Cuts at: 1548 3072 1548 Size: 1524 4780 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4915 136 Size: 107 122 71 631 605 3243 1525 Fragments arranged by size: 3243 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6299 6299 Size: 6304 BlpI GC'TnA_GC Cuts at: 2639 3721 2639 Size: 1082 5222 Bme1580I G_kGCm'C Cuts at: 1790 4365 5521 1790 Size: 2575 1156 2573 Fragments arranged by size: 2575 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6304 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4035 4993 442 Size: 416 53 83 399 24 2618 958 1753 Fragments arranged by size: 2618 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6304 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2332 2851 2332 Size: 519 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2005 2083 2556 2669 2807 2987 4964 1239 Size: 766 78 473 113 138 180 1977 2579 Fragments arranged by size: 2579 1977 766 473 180 138 113 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2302 4429 5294 5592 2302 Size: 2127 865 298 3014 Fragments arranged by size: 3014 2127 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2191 2233 2570 4967 882 Size: 617 692 42 337 2397 2219 Fragments arranged by size: 2397 2219 692 617 337 42 BsaAI yAC'GTr Cuts at: 493 1204 2910 4095 493 Size: 711 1706 1185 2702 Fragments arranged by size: 2702 1706 1185 711 BsaBI GATnn'nnATC Cuts at: 6298 6298 Size: 6304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2178 2610 3498 3536 Size: 53 83 186 936 645 432 888 38 Cuts at: 3536 4111 4493 5128 275 Size: 575 382 635 1451 Fragments arranged by size: 1451 936 888 645 635 575 432 382 186 83 53 38 BsaWI w'CCGG_w Cuts at: 4737 5135 5409 5556 5967 4737 Size: 398 274 147 411 5074 Fragments arranged by size: 5074 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 2642 2672 3011 Size: 30 165 30 678 30 531 30 339 Cuts at: 3011 3041 1178 Size: 30 4441 Fragments arranged by size: 4441 678 531 339 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2008 2468 2520 3179 3446 3491 1186 Size: 822 460 52 659 267 45 3999 Fragments arranged by size: 3999 822 659 460 267 52 45 BseYI C'CCAG_C Cuts at: 5507 6003 5507 Size: 496 5808 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5731 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3292 3754 4515 5543 1540 Size: 275 186 1291 462 761 1028 2301 Fragments arranged by size: 2301 1291 1028 761 462 275 186 BsiHKAI G_wGCw'C Cuts at: 2655 3171 4365 4450 5521 2655 Size: 516 1194 85 1071 3438 Fragments arranged by size: 3438 1194 1071 516 85 BsmI GAATG_Cn' Cuts at: 2064 2324 3815 3908 2064 Size: 260 1491 93 4460 Fragments arranged by size: 4460 1491 260 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2628 2655 3171 3623 4365 4450 5521 Size: 399 838 27 516 452 742 85 1071 Cuts at: 5521 1391 Size: 2174 Fragments arranged by size: 2174 1071 838 742 516 452 399 85 27 BspEI T'CCGG_A Cuts at: 5967 5967 Size: 6304 BspHI T'CATG_A Cuts at: 4193 5923 4193 Size: 1730 4574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2290 2571 3659 844 Size: 1446 281 1088 3489 Fragments arranged by size: 3489 1446 1088 281 BsrBI CCG'CTC Cuts at: 1394 1823 4191 1394 Size: 429 2368 3507 Fragments arranged by size: 3507 2368 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4799 4973 65 Size: 1183 3551 174 1396 Fragments arranged by size: 3551 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2028 2132 4948 5135 1700 Size: 110 218 104 2816 187 2869 Fragments arranged by size: 2869 2816 218 187 110 104 BsrGI T'GTAC_A Cuts at: 96 2796 96 Size: 2700 3604 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6304 BssSI C'ACGA_G Cuts at: 4364 5376 4364 Size: 1012 5292 BstAPI GCAn_nnn'nTGC Cuts at: 794 2091 3107 794 Size: 1297 1016 3991 Fragments arranged by size: 3991 1297 1016 BstBI TT'CG_AA Cuts at: 5108 5108 Size: 6304 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6304 BstXI CCAn_nnnn'nTGG Cuts at: 2047 3097 2047 Size: 1050 5254 BstYI r'GATC_y Cuts at: 1972 2009 2997 3057 3634 4001 4386 4403 5855 Size: 37 988 60 577 367 385 17 1452 Cuts at: 5855 5941 5953 6299 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 988 577 385 367 346 86 60 37 17 12 Bsu36I CC'TnA_GG Cuts at: 2708 2708 Size: 6304 BtgI C'CryG_G Cuts at: 513 1065 2004 2181 2985 3423 513 Size: 552 939 177 804 438 3394 Fragments arranged by size: 3394 939 804 552 438 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2110 2553 487 Size: 683 56 884 443 4238 Fragments arranged by size: 4238 884 683 443 56 BtsI GCAGTG_nn' Cuts at: 2770 2977 3820 4614 4634 2770 Size: 207 843 794 20 4440 Fragments arranged by size: 4440 843 794 207 20 ClaI AT'CG_AT Cuts at: 1554 2055 1554 Size: 501 5803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6269 DraI TTT'AAA Cuts at: 3608 3608 Size: 6304 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2183 5311 809 Size: 507 867 3128 1802 Fragments arranged by size: 3128 1802 867 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2247 2463 2868 Size: 54 1425 50 275 18 417 216 405 Cuts at: 2868 3087 3751 4640 6269 8 Size: 219 664 889 1629 43 Fragments arranged by size: 1629 1425 889 664 417 405 275 219 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3751 1537 Size: 275 1939 4090 Fragments arranged by size: 4090 1939 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3422 4234 1353 Size: 2069 812 3423 Fragments arranged by size: 3423 2069 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4891 5265 5411 387 Size: 1064 138 3302 374 146 1280 Fragments arranged by size: 3302 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2005 2065 2083 2370 2556 2669 2671 Size: 393 373 60 18 287 186 113 2 Cuts at: 2671 2807 2987 3203 3503 4367 4964 5751 6019 Size: 136 180 216 300 864 597 787 268 Cuts at: 6019 1239 Size: 1524 Fragments arranged by size: 1524 864 787 597 393 373 300 287 268 216 186 180 136 113 60 18 2 EcoO109I rG'GnC_Cy Cuts at: 2587 2587 Size: 6304 EcoRI G'AATT_C Cuts at: 2212 3613 3991 2212 Size: 1401 378 4525 Fragments arranged by size: 4525 1401 378 EcoRV GAT'ATC Cuts at: 2848 3211 2848 Size: 363 5941 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2095 2620 2095 Size: 525 5779 FspI TGC'GCA Cuts at: 4810 4810 Size: 6304 HaeII r_GCGC'y Cuts at: 1678 5451 1678 Size: 3773 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4945 4977 5019 5051 1373 Size: 32 135 32 3373 32 42 32 2626 Fragments arranged by size: 3373 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2526 3648 3894 669 Size: 988 281 588 1122 246 3079 Fragments arranged by size: 3079 1122 988 588 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6304 HpaI GTT'AAC Cuts at: 3894 3894 Size: 6304 KpnI G_GTAC'C Cuts at: 3629 3629 Size: 6304 MfeI C'AATT_G Cuts at: 3903 3903 Size: 6304 MluI A'CGCG_T Cuts at: 2383 6180 2383 Size: 3797 2507 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3054 3085 5418 5602 1305 Size: 1749 31 2333 184 2007 Fragments arranged by size: 2333 2007 1749 184 31 MscI TGG'CCA Cuts at: 10 64 1489 1832 2249 3089 10 Size: 54 1425 343 417 840 3225 Fragments arranged by size: 3225 1425 840 417 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3095 4262 4621 4780 518 Size: 648 1929 1167 359 159 2042 Fragments arranged by size: 2042 1929 1167 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2867 2965 3720 4397 5545 5790 1606 Size: 1261 98 755 677 1148 245 2120 Fragments arranged by size: 2120 1261 1148 755 677 245 98 NcoI C'CATG_G Cuts at: 513 1065 2004 2181 3423 513 Size: 552 939 177 1242 3394 Fragments arranged by size: 3394 1242 939 552 177 NdeI CA'TA_TG Cuts at: 387 387 Size: 6304 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6304 NotI GC'GGCC_GC Cuts at: 3751 3751 Size: 6304 NspI r_CATG'y Cuts at: 1132 1229 2659 3110 3662 5207 1132 Size: 97 1430 451 552 1545 2229 Fragments arranged by size: 2229 1545 1430 552 451 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6304 PciI A'CATG_T Cuts at: 2655 5203 2655 Size: 2548 3756 PflMI CCAn_nnn'nTGG Cuts at: 1108 2884 3044 1108 Size: 1776 160 4368 Fragments arranged by size: 4368 1776 160 PfoI T'CCnGG_A Cuts at: 2823 2823 Size: 6304 PmeI GTTT'AAAC Cuts at: 3608 3608 Size: 6304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4373 4405 5886 5918 4373 Size: 32 1481 32 4759 Fragments arranged by size: 4759 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6304 PsiI TTA'TAA Cuts at: 3874 3874 Size: 6304 PstI C_TGCA'G Cuts at: 830 2819 3447 3656 830 Size: 1989 628 209 3478 Fragments arranged by size: 3478 1989 628 209 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6304 RsrII CG'GwC_CG Cuts at: 2941 2941 Size: 6304 SalI G'TCGA_C Cuts at: 1936 3646 1936 Size: 1710 4594 SapI GCTCTTCn'nnn_ Cuts at: 3422 3422 Size: 6304 SbfI CC_TGCA'GG Cuts at: 3656 3656 Size: 6304 ScaI AGT'ACT Cuts at: 2090 4552 6264 2090 Size: 2462 1712 2130 Fragments arranged by size: 2462 2130 1712 SfcI C'TryA_G Cuts at: 826 1045 2740 2815 3154 3443 3652 4787 5468 Size: 219 1695 75 339 289 209 1135 681 Cuts at: 5468 5659 826 Size: 191 1471 Fragments arranged by size: 1695 1471 1135 681 339 289 219 209 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6304 SmaI CCC'GGG Cuts at: 1872 3631 1872 Size: 1759 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2317 4408 5309 5571 820 Size: 197 926 374 2091 901 262 1553 Fragments arranged by size: 2091 1553 926 901 374 262 197 SnaBI TAC'GTA Cuts at: 493 4095 493 Size: 3602 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6304 SphI G_CATG'C Cuts at: 1132 3662 1132 Size: 2530 3774 SspI AAT'ATT Cuts at: 5 52 3275 4228 5 Size: 47 3223 953 2081 Fragments arranged by size: 3223 2081 953 47 StuI AGG'CCT Cuts at: 1517 3309 1517 Size: 1792 4512 StyI C'CwwG_G Cuts at: 513 1065 2004 2034 2181 3248 3348 3423 3743 Size: 552 939 30 147 1067 100 75 320 Cuts at: 3743 513 Size: 3074 Fragments arranged by size: 3074 1067 939 552 320 147 100 75 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3241 3348 4331 4348 4501 4686 5131 Size: 1255 1661 107 983 17 153 185 445 Cuts at: 5131 325 Size: 1498 Fragments arranged by size: 1661 1498 1255 983 445 185 153 107 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2088 2496 2796 Size: 274 80 33 51 1443 111 408 300 Cuts at: 2796 4550 6262 96 Size: 1754 1712 138 Fragments arranged by size: 1754 1712 1443 408 300 274 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2391 2423 6264 6296 2391 Size: 32 3841 32 2399 Fragments arranged by size: 3841 2399 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6304 XbaI T'CTAG_A Cuts at: 3640 3640 Size: 6304 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2107 2107 Size: 6304 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6304 XmaI C'CCGG_G Cuts at: 1870 3629 1870 Size: 1759 4545 XmnI GAAnn'nnTTC Cuts at: 3309 4433 3309 Size: 1124 5180 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2611 3537 4112 276 Size: 53 83 186 936 1077 926 575 2468 Fragments arranged by size: 2468 1077 936 926 575 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI RsrII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI