Restriction map for
pFN21AB9914
(Linear) (Six-base) MAPSORT of: KIBB9914.seq Check: 3115 from: 1 to: 1599
KIBB9914.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AatII G_ACGT'C
Cuts at: 0 608 1534 1599
Size: 608 926 65
Fragments arranged by size:
926 608 65
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 365 666 1198 1498 1599
Size: 60 305 301 532 300 101
Fragments arranged by size:
532 305 301 300 101 60
AflIII A'CryG_T
Cuts at: 0 378 650 902 1599
Size: 378 272 252 697
Fragments arranged by size:
697 378 272 252
ApoI r'AATT_y
Cuts at: 0 207 1140 1599
Size: 207 933 459
Fragments arranged by size:
933 459 207
AseI AT'TA_AT
Cuts at: 0 274 1075 1599
Size: 274 801 524
Fragments arranged by size:
801 524 274
AvaI C'yCGr_G
Cuts at: 0 664 1457 1599
Size: 664 793 142
Fragments arranged by size:
793 664 142
BamHI G'GATC_C
Cuts at: 0 4 1599
Size: 4 1595
BanI G'GyrC_C
Cuts at: 0 1314 1599
Size: 1314 285
BanII G_rGCy'C
Cuts at: 0 623 1599
Size: 623 976
BbsI GAAGACnn'nnnn_
Cuts at: 0 1522 1599
Size: 1522 77
BclI T'GATC_A
Cuts at: 0 1067 1599
Size: 1067 532
BlpI GC'TnA_GC
Cuts at: 0 634 1599
Size: 634 965
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 327 846 1599
Size: 327 519 753
Fragments arranged by size:
753 519 327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 551 664 802 982 1599
Size: 78 473 113 138 180 617
Fragments arranged by size:
617 473 180 138 113 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 1599
Size: 297 1302
BsaI GGTCTCn'nnnn_
Cuts at: 0 186 228 565 1599
Size: 186 42 337 1034
Fragments arranged by size:
1034 337 186 42
BsaAI yAC'GTr
Cuts at: 0 905 1599
Size: 905 694
BsaHI Gr'CG_yC
Cuts at: 0 173 605 1493 1531 1599
Size: 173 432 888 38 68
Fragments arranged by size:
888 432 173 68 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 637 667 1006 1036 1599
Size: 76 30 531 30 339 30 563
Fragments arranged by size:
563 531 339 76 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3 463 515 1174 1441 1486 1599
Size: 3 460 52 659 267 45 113
Fragments arranged by size:
659 460 267 113 52 45 3
BsiEI CG_ry'CG
Cuts at: 0 1287 1599
Size: 1287 312
BsiHKAI G_wGCw'C
Cuts at: 0 650 1166 1599
Size: 650 516 433
Fragments arranged by size:
650 516 433
BsmI GAATG_Cn'
Cuts at: 0 59 319 1599
Size: 59 260 1280
Fragments arranged by size:
1280 260 59
Bsp1286I G_dGCh'C
Cuts at: 0 623 650 1166 1599
Size: 623 27 516 433
Fragments arranged by size:
623 516 433 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 285 566 1599
Size: 285 281 1033
Fragments arranged by size:
1033 285 281
BsrFI r'CCGG_y
Cuts at: 0 23 127 1599
Size: 23 104 1472
Fragments arranged by size:
1472 104 23
BsrGI T'GTAC_A
Cuts at: 0 791 1599
Size: 791 808
BstAPI GCAn_nnn'nTGC
Cuts at: 0 86 1102 1599
Size: 86 1016 497
Fragments arranged by size:
1016 497 86
BstXI CCAn_nnnn'nTGG
Cuts at: 0 42 1092 1599
Size: 42 1050 507
Fragments arranged by size:
1050 507 42
BstYI r'GATC_y
Cuts at: 0 4 992 1052 1599
Size: 4 988 60 547
Fragments arranged by size:
988 547 60 4
Bsu36I CC'TnA_GG
Cuts at: 0 703 1599
Size: 703 896
BtgI C'CryG_G
Cuts at: 0 176 980 1418 1599
Size: 176 804 438 181
Fragments arranged by size:
804 438 181 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 105 548 1599
Size: 105 443 1051
Fragments arranged by size:
1051 443 105
BtsI GCAGTG_nn'
Cuts at: 0 765 972 1599
Size: 765 207 627
Fragments arranged by size:
765 627 207
ClaI AT'CG_AT
Cuts at: 0 50 1599
Size: 50 1549
DrdI GACnn_nn'nnGTC
Cuts at: 0 178 1599
Size: 178 1421
EaeI y'GGCC_r
Cuts at: 0 242 458 863 1082 1599
Size: 242 216 405 219 517
Fragments arranged by size:
517 405 242 219 216
EarI CTCTTCn'nnn_
Cuts at: 0 1417 1599
Size: 1417 182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 78 365 551 664 666 802 982
Size: 60 18 287 186 113 2 136 180
Cuts at: 982 1198 1498 1599
Size: 216 300 101
Fragments arranged by size:
300 287 216 186 180 136 113 101
60 18 2
EcoO109I rG'GnC_Cy
Cuts at: 0 582 1599
Size: 582 1017
EcoRI G'AATT_C
Cuts at: 0 207 1599
Size: 207 1392
EcoRV GAT'ATC
Cuts at: 0 843 1206 1599
Size: 843 363 393
Fragments arranged by size:
843 393 363
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 90 615 1599
Size: 90 525 984
Fragments arranged by size:
984 525 90
HincII GTy'rAC
Cuts at: 0 521 1599
Size: 521 1078
MluI A'CGCG_T
Cuts at: 0 378 1599
Size: 378 1221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1049 1080 1599
Size: 1049 31 519
Fragments arranged by size:
1049 519 31
MscI TGG'CCA
Cuts at: 0 244 1084 1599
Size: 244 840 515
Fragments arranged by size:
840 515 244
MslI CAynn'nnrTG
Cuts at: 0 1090 1599
Size: 1090 509
MspA1I CmG'CkG
Cuts at: 0 862 960 1599
Size: 862 98 639
Fragments arranged by size:
862 639 98
NcoI C'CATG_G
Cuts at: 0 176 1418 1599
Size: 176 1242 181
Fragments arranged by size:
1242 181 176
NspI r_CATG'y
Cuts at: 0 654 1105 1599
Size: 654 451 494
Fragments arranged by size:
654 494 451
PciI A'CATG_T
Cuts at: 0 650 1599
Size: 650 949
PflMI CCAn_nnn'nTGG
Cuts at: 0 879 1039 1599
Size: 879 160 560
Fragments arranged by size:
879 560 160
PfoI T'CCnGG_A
Cuts at: 0 818 1599
Size: 818 781
PstI C_TGCA'G
Cuts at: 0 814 1442 1599
Size: 814 628 157
Fragments arranged by size:
814 628 157
RsrII CG'GwC_CG
Cuts at: 0 936 1599
Size: 936 663
SapI GCTCTTCn'nnn_
Cuts at: 0 1417 1599
Size: 1417 182
ScaI AGT'ACT
Cuts at: 0 85 1599
Size: 85 1514
SfcI C'TryA_G
Cuts at: 0 735 810 1149 1438 1599
Size: 735 75 339 289 161
Fragments arranged by size:
735 339 289 161 75
SmlI C'TyrA_G
Cuts at: 0 312 1599
Size: 312 1287
SspI AAT'ATT
Cuts at: 0 1270 1599
Size: 1270 329
StuI AGG'CCT
Cuts at: 0 1304 1599
Size: 1304 295
StyI C'CwwG_G
Cuts at: 0 29 176 1243 1343 1418 1599
Size: 29 147 1067 100 75 181
Fragments arranged by size:
1067 181 147 100 75 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1236 1343 1599
Size: 1236 107 256
Fragments arranged by size:
1236 256 107
TatI w'GTAC_w
Cuts at: 0 83 491 791 1599
Size: 83 408 300 808
Fragments arranged by size:
808 408 300 83
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 386 418 1599
Size: 386 32 1181
Fragments arranged by size:
1181 386 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 102 1599
Size: 102 1497
XmnI GAAnn'nnTTC
Cuts at: 0 1304 1599
Size: 1304 295
ZraI GAC'GTC
Cuts at: 0 606 1532 1599
Size: 606 926 67
Fragments arranged by size:
926 606 67
Enzymes that do cut:
AatII AcuI AflIII ApoI AseI AvaI BamHI BanI
BanII BbsI BclI BlpI BplI BpmI BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DrdI EaeI EarI Eco57MI EcoO109I
EcoRI EcoRV FalI HincII MluI MmeI MscI MslI
MspA1I NcoI NspI PciI PflMI PfoI PstI RsrII
SapI ScaI SfcI SmlI SspI StuI StyI TaqII
TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII Bme1580I BmgBI BmrI BmtI Bpu10I BsaBI BsaWI
BseYI BsgI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BssHII BssSI BstBI BstEII BstZ17I CspCI DraI DraIII
EagI EciI EcoICRI EcoNI FseI FspI FspAI HaeII
Hin4I HindIII HpaI KasI KpnI MfeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII SacI SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XhoI XmaI