Restriction map for
pFN21AB7959
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpmI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstYI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
EarI Eco47III EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MroI MseI MunI
NarI NcoI NdeI NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 127 ACGCGT A^C[GA][CT]GT
AocI 22 CCTCAGG CC^T[ACGT]AGG
Asp700I 12 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 550 CCCGAG C^[CT]CG[GA]G
BalI 350 TGGCCA TGG^CCA
BanI 679 GGCACC G^G[CT][GA]CC
BanII 228 GGGCTC G[GA]GC[CT]^C
BbsI 733 GAAGAC GAAGAC(2/6)
BglI 212 GCCCGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 72 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 72 GCTCAGC GC^T[ACGT]AGC
BpuAI 733 GAAGAC GAAGAC(2/6)
BsoBI 550 CCCGAG C^[CT]CG[GA]G
BsrBI 545 CCGCTC CCGCTC(-3/-3)
BsrI 353 CCAGT ACTGG(1/-1)
BstEII 540 GGTCACC G^GT[ACGT]ACC
BstPI 540 GGTCACC G^GT[ACGT]ACC
Bsu36I 22 CCTCAGG CC^T[ACGT]AGG
CelII 72 GCTCAGC GC^T[ACGT]AGC
DpnII 441 GATC ^GATC
DraIII 379 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 215 CGGCCG C^GGCCG
EclXI 215 CGGCCG C^GGCCG
Eco52I 215 CGGCCG C^GGCCG
Eco81I 22 CCTCAGG CC^T[ACGT]AGG
EcoO65I 540 GGTCACC G^GT[ACGT]ACC
FauI 168 GCGGG CCCGC(4/6)
HinfI 271 GACTC G^A[ACGT]TC
MaeII 116 ACGT A^CGT
MboI 441 GATC ^GATC
MboII 733 GAAGA GAAGA(8/7)
MluI 127 ACGCGT A^CGCGT
MluNI 350 TGGCCA TGG^CCA
MscI 350 TGGCCA TGG^CCA
NdeII 441 GATC ^GATC
PleI 271 GACTC GAGTC(4/5)
Sau3AI 441 GATC ^GATC
SmlI 328 CTCAAG C^T[CT][GA]AG
TspRI 354 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 765 CCAGGAGGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 12 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 256 AAATTC [GA]^AATT[CT]
295 GAATTT
AfaI 318 GTAC GT^AC
390 GTAC
ApoI 256 AAATTC [GA]^AATT[CT]
295 GAATTT
AspHI 112 GAGCAC G[AT]GC[AT]^C
496 GAGCAC
AvaII 179 GGTCC G^G[AT]CC
474 GGTCC
BseRI 231 CTCCTC GAGGAG(10/8)
688 CTCCTC
BsiEI 215 CGGCCG CG[GA][CT]^CG
279 CGACCG
BsiHKAI 112 GAGCAC G[AT]GC[AT]^C
496 GAGCAC
BsmFI 180 GTCCC GGGAC(10/14)
597 GTCCC
BspMI 507 GCAGGT ACCTGC(4/8)
537 GCAGGT
BstF5I 567 CATCC GGATG(2/0)
771 GGATG
CfoI 674 GCGC GCG^C
703 GCGC
DsaI 120 CCGTGG C^C[GA][CT]GG
638 CCGTGG
EaeI 215 CGGCCG [CT]^GGCC[GA]
350 TGGCCA
EcoNI 22 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
723 CCTCTCCAAGG
EcoT14I 101 CCAAGG C^C[AT][AT]GG
728 CCAAGG
FokI 567 CATCC GGATG(9/13)
771 GGATG
HhaI 674 GCGC GCG^C
703 GCGC
HinP1I 674 GCGC G^CGC
703 GCGC
PpuMI 178 GGGTCCC [GA]G^G[AT]CC[CT]
473 AGGTCCT
PvuII 592 CAGCTG CAG^CTG
601 CAGCTG
RsaI 318 GTAC GT^AC
390 GTAC
StyI 101 CCAAGG C^C[AT][AT]GG
728 CCAAGG
Tsp509I 257 AATT ^AATT
296 AATT
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AluI AlwNI BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BsrFI BssKI BstNI BstUI
Cac8I Cfr10I Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HgaI HpaII HphI ItaI MaeIII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NgoMIV
NlaIV PstI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I TthHB8I