(Circular) (Six-base) MAPSORT of: pFN21AB7959.seq Check: 9558 from: 1 to: 5497 pFN21AB7959 5497 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3307 278 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2840 1937 Size: 903 4594 Acc65I G'GTAC_C Cuts at: 2818 2818 Size: 5497 AclI AA'CG_TT Cuts at: 1563 3624 3997 1563 Size: 2061 373 3063 Fragments arranged by size: 3063 2061 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3560 4944 5212 1632 Size: 1928 1384 268 1917 Fragments arranged by size: 1928 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5300 AflIII A'CryG_T Cuts at: 1203 2132 4396 5373 1203 Size: 929 2264 977 1327 Fragments arranged by size: 2264 1327 977 929 AgeI A'CCGG_T Cuts at: 4328 4328 Size: 5497 AhdI GACnn_n'nnGTC Cuts at: 4226 4226 Size: 5497 AleI CACnn'nnGTG Cuts at: 1166 2124 2757 1166 Size: 958 633 3906 Fragments arranged by size: 3906 958 633 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5497 AlwNI CAG_nnn'CTG Cuts at: 2311 2482 2617 3240 4812 2311 Size: 171 135 623 1572 2996 Fragments arranged by size: 2996 1572 623 171 135 ApaLI G'TGCA_C Cuts at: 3554 4710 3554 Size: 1156 4341 ApoI r'AATT_y Cuts at: 1493 2261 2300 2806 3032 3184 5293 1493 Size: 768 39 506 226 152 2109 1697 Fragments arranged by size: 2109 1697 768 506 226 152 39 AseI AT'TA_AT Cuts at: 160 1032 4051 160 Size: 872 3019 1606 Fragments arranged by size: 3019 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 AvaI C'yCGr_G Cuts at: 1870 1943 2555 2822 1870 Size: 73 612 267 4545 Fragments arranged by size: 4545 612 267 73 BamHI G'GATC_C Cuts at: 2827 3194 2827 Size: 367 5130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2684 2818 4273 618 Size: 325 217 627 897 134 1455 1842 Fragments arranged by size: 1842 1455 897 627 325 217 134 BanII G_rGCy'C Cuts at: 1391 2237 2816 1391 Size: 846 579 4072 Fragments arranged by size: 4072 846 579 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2745 928 Size: 971 846 3680 Fragments arranged by size: 3680 971 846 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3688 3722 1903 Size: 34 1751 34 3678 Fragments arranged by size: 3678 1751 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3389 4599 3389 Size: 1210 4287 BclI T'GATC_A Cuts at: 1548 1548 Size: 5497 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2223 4108 136 Size: 107 122 71 631 605 551 1885 1525 Fragments arranged by size: 1885 1525 631 605 551 122 107 71 BglII A'GATC_T Cuts at: 5492 5492 Size: 5497 BlpI GC'TnA_GC Cuts at: 2078 2914 2078 Size: 836 4661 Bme1580I G_kGCm'C Cuts at: 1790 2476 2754 3558 4714 1790 Size: 686 278 804 1156 2573 Fragments arranged by size: 2573 1156 804 686 278 BmgBI CAC'GTC Cuts at: 1318 2122 1318 Size: 804 4693 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3228 4186 442 Size: 416 53 83 399 24 1811 958 1753 Fragments arranged by size: 1811 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5497 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2581 2581 Size: 5497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4157 1239 Size: 2918 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2318 3622 4487 4785 2318 Size: 1304 865 298 3030 Fragments arranged by size: 3030 1304 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4160 882 Size: 617 2661 2219 Fragments arranged by size: 2661 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3288 493 Size: 711 2084 2702 Fragments arranged by size: 2702 2084 711 BsaBI GATnn'nnATC Cuts at: 5491 5491 Size: 5497 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3304 3686 4321 275 Size: 53 83 186 936 1771 382 635 1451 Fragments arranged by size: 1771 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3930 4328 4602 4749 5160 3930 Size: 398 274 147 411 4267 Fragments arranged by size: 4267 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2568 2598 1178 Size: 30 165 30 1165 30 4077 Fragments arranged by size: 4077 1165 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2227 2684 1186 Size: 1041 457 3999 Fragments arranged by size: 3999 1041 457 BseYI C'CCAG_C Cuts at: 2095 2250 2604 2710 4700 5196 2095 Size: 155 354 106 1990 496 2396 Fragments arranged by size: 2396 1990 496 354 155 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4924 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2223 2287 2947 3708 4736 1540 Size: 275 186 222 64 660 761 1028 2301 Fragments arranged by size: 2301 1028 761 660 275 222 186 64 BsiHKAI G_wGCw'C Cuts at: 2121 2505 3558 3643 4714 2121 Size: 384 1053 85 1071 2904 Fragments arranged by size: 2904 1071 1053 384 85 BsmI GAATG_Cn' Cuts at: 3008 3101 3008 Size: 93 5404 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2237 2476 2505 2754 2816 3558 Size: 399 331 116 239 29 249 62 742 Cuts at: 3558 3643 4714 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 331 249 239 116 85 62 29 BspEI T'CCGG_A Cuts at: 5160 5160 Size: 5497 BspHI T'CATG_A Cuts at: 3386 5116 3386 Size: 1730 3767 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2503 2533 2852 844 Size: 1659 30 319 3489 Fragments arranged by size: 3489 1659 319 30 BsrBI CCG'CTC Cuts at: 1394 1823 2552 3384 1394 Size: 429 729 832 3507 Fragments arranged by size: 3507 832 729 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3992 4166 65 Size: 1183 2744 174 1396 Fragments arranged by size: 2744 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2158 2222 2668 2687 4141 4328 1700 Size: 110 348 64 446 19 1454 187 2869 Fragments arranged by size: 2869 1454 446 348 187 110 64 19 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5497 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5497 BssSI C'ACGA_G Cuts at: 3557 4569 3557 Size: 1012 4485 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5497 BstBI TT'CG_AA Cuts at: 4301 4301 Size: 5497 BstEII G'GTnAC_C Cuts at: 1184 2545 1184 Size: 1361 4136 BstYI r'GATC_y Cuts at: 1972 2827 3194 3579 3596 5048 5134 5146 5492 Size: 855 367 385 17 1452 86 12 346 Cuts at: 5492 1972 Size: 1977 Fragments arranged by size: 1977 1452 855 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2028 2028 Size: 5497 BtgI C'CryG_G Cuts at: 513 1065 2125 2643 513 Size: 552 1060 518 3367 Fragments arranged by size: 3367 1060 552 518 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4758 Fragments arranged by size: 4758 683 56 BtsI GCAGTG_nn' Cuts at: 3013 3807 3827 3013 Size: 794 20 4683 Fragments arranged by size: 4683 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5497 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5462 DraI TTT'AAA Cuts at: 2801 2801 Size: 5497 DraIII CAC_nnn'GTG Cuts at: 2389 2389 Size: 5497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4504 809 Size: 507 3188 1802 Fragments arranged by size: 3188 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 2355 2944 Size: 54 1425 50 275 18 390 135 589 Cuts at: 2944 3833 5462 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 589 390 275 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2220 2944 1537 Size: 275 408 724 4090 Fragments arranged by size: 4090 724 408 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3427 1353 Size: 2074 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4084 4458 4604 387 Size: 1064 138 2495 374 146 1280 Fragments arranged by size: 2495 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3560 4157 4944 5212 1239 Size: 393 1928 597 787 268 1524 Fragments arranged by size: 1928 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2031 2732 2031 Size: 701 4796 EcoO109I rG'GnC_Cy Cuts at: 2184 2479 2614 2184 Size: 295 135 5067 Fragments arranged by size: 5067 295 135 EcoRI G'AATT_C Cuts at: 2806 3184 2806 Size: 378 5119 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2038 2038 Size: 5497 FspI TGC'GCA Cuts at: 4003 4003 Size: 5497 HaeII r_GCGC'y Cuts at: 1678 4644 1678 Size: 2966 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2094 2126 2365 2397 2568 Size: 32 135 32 522 32 239 32 171 Cuts at: 2568 2600 4138 4170 4212 4244 1373 Size: 32 1538 32 42 32 2626 Fragments arranged by size: 2626 1538 522 239 171 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2841 3087 669 Size: 988 281 903 246 3079 Fragments arranged by size: 3079 988 903 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5497 HpaI GTT'AAC Cuts at: 3087 3087 Size: 5497 KpnI G_GTAC'C Cuts at: 2822 2822 Size: 5497 MfeI C'AATT_G Cuts at: 3096 3096 Size: 5497 MluI A'CGCG_T Cuts at: 2132 5373 2132 Size: 3241 2256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2599 2772 4611 4795 1305 Size: 1294 173 1839 184 2007 Fragments arranged by size: 2007 1839 1294 184 173 MscI TGG'CCA Cuts at: 10 64 1489 1832 2357 10 Size: 54 1425 343 525 3150 Fragments arranged by size: 3150 1425 525 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2016 2124 2426 2757 3455 3814 3973 Size: 648 850 108 302 331 698 359 159 Cuts at: 3973 518 Size: 2042 Fragments arranged by size: 2042 850 698 648 359 331 302 159 108 MspA1I CmG'CkG Cuts at: 1606 2048 2599 2608 2913 3590 4738 4983 1606 Size: 442 551 9 305 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 551 442 305 245 9 NaeI GCC'GGC Cuts at: 2160 2224 2670 2160 Size: 64 446 4987 Fragments arranged by size: 4987 446 64 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4945 NdeI CA'TA_TG Cuts at: 387 387 Size: 5497 NgoMIV G'CCGG_C Cuts at: 2158 2222 2668 2158 Size: 64 446 4987 Fragments arranged by size: 4987 446 64 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5497 NotI GC'GGCC_GC Cuts at: 2944 2944 Size: 5497 NspI r_CATG'y Cuts at: 1132 1229 2855 4400 1132 Size: 97 1626 1545 2229 Fragments arranged by size: 2229 1626 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5497 PciI A'CATG_T Cuts at: 4396 4396 Size: 5497 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5497 PmeI GTTT'AAAC Cuts at: 2801 2801 Size: 5497 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3566 3598 5079 5111 3566 Size: 32 1481 32 3952 Fragments arranged by size: 3952 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2184 2479 2184 Size: 295 5202 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5497 PsiI TTA'TAA Cuts at: 3067 3067 Size: 5497 PstI C_TGCA'G Cuts at: 830 2061 2613 2655 2849 830 Size: 1231 552 42 194 3478 Fragments arranged by size: 3478 1231 552 194 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5497 PvuII CAG'CTG Cuts at: 2599 2608 2599 Size: 9 5488 SalI G'TCGA_C Cuts at: 1936 2839 1936 Size: 903 4594 SanDI GG'GwC_CC Cuts at: 2184 2184 Size: 5497 SbfI CC_TGCA'GG Cuts at: 2849 2849 Size: 5497 ScaI AGT'ACT Cuts at: 3745 5457 3745 Size: 1712 3785 SfcI C'TryA_G Cuts at: 826 1045 2057 2609 2651 2845 3980 4661 4852 Size: 219 1012 552 42 194 1135 681 191 Cuts at: 4852 826 Size: 1471 Fragments arranged by size: 1471 1135 1012 681 552 219 194 191 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5497 SmaI CCC'GGG Cuts at: 1872 2824 1872 Size: 952 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2333 3601 4502 4764 820 Size: 197 926 390 1268 901 262 1553 Fragments arranged by size: 1553 1268 926 901 390 262 197 SnaBI TAC'GTA Cuts at: 493 3288 493 Size: 2795 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5497 SphI G_CATG'C Cuts at: 1132 2855 1132 Size: 1723 3774 SspI AAT'ATT Cuts at: 5 52 3421 5 Size: 47 3369 2081 Fragments arranged by size: 3369 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5497 StyI C'CwwG_G Cuts at: 513 1065 2106 2733 2936 513 Size: 552 1041 627 203 3074 Fragments arranged by size: 3074 1041 627 552 203 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2780 3524 3541 3694 3879 4324 325 Size: 1255 1200 744 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1200 744 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2394 3743 5455 Size: 274 80 33 51 1443 417 1349 1712 Cuts at: 5455 96 Size: 138 Fragments arranged by size: 1712 1443 1349 417 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2585 2617 5457 5489 2585 Size: 32 2840 32 2593 Fragments arranged by size: 2840 2593 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5497 XbaI T'CTAG_A Cuts at: 2833 2833 Size: 5497 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2777 2777 Size: 5497 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5497 XmaI C'CCGG_G Cuts at: 1870 2822 1870 Size: 952 4545 XmnI GAAnn'nnTTC Cuts at: 2021 3626 2021 Size: 1605 3892 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3305 276 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI