Restriction map for
pFN21AB7957
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BanI BbeI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BseAI BsiHKAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MspA1I MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 485 CGCG CG^CG
AfaI 474 GTAC GT^AC
AlwNI 508 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 163 CTCGGG C^[CT]CG[GA]G
BalI 260 TGGCCA TGG^CCA
BamHI 446 GGATCC G^GATCC
BanII 565 GAGCCC G[GA]GC[CT]^C
BbrPI 256 CACGTG CAC^GTG
BmyI 565 GAGCCC G[AGT]GC[ACT]^C
BsaAI 256 CACGTG [CT]AC^GT[GA]
BsaI 718 GGTCTC GGTCTC(1/5)
BsaWI 428 ACCGGA [AT]^CCGG[AT]
BseRI 697 GAGGAG GAGGAG(10/8)
BsgI 628 GTGCAG GTGCAG(16/14)
BsiEI 143 CGGCCG CG[GA][CT]^CG
BsmBI 183 CGTCTC CGTCTC(1/5)
BsoBI 163 CTCGGG C^[CT]CG[GA]G
Bsp1286I 565 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 473 TGTACA T^GTACA
BspMI 133 GCAGGT ACCTGC(4/8)
BsrBI 592 CCGCTC CCGCTC(-3/-3)
BsrGI 473 TGTACA T^GTACA
BstUI 485 CGCG CG^CG
BstYI 446 GGATCC [GA]^GATC[CT]
CfoI 486 GCGC GCG^C
EagI 143 CGGCCG C^GGCCG
EarI 704 CTCTTC CTCTTC(1/4)
EclXI 143 CGGCCG C^GGCCG
Eco52I 143 CGGCCG C^GGCCG
EcoNI 555 CCTACTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 591 CCCGC CCCGC(4/6)
HgaI 251 GCGTC GACGC(5/10)
HhaI 486 GCGC GCG^C
HinP1I 486 GCGC G^CGC
HindIII 42 AAGCTT A^AGCTT
Ksp632I 704 CTCTTC CTCTTC(1/4)
MaeII 257 ACGT A^CGT
MflI 446 GGATCC [GA]^GATC[CT]
MluNI 260 TGGCCA TGG^CCA
MscI 260 TGGCCA TGG^CCA
MslI 105 CACAGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 485 CGCG CG^CG
MwoI 404 GCCTCAGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 507 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 256 CACGTG CAC^GTG
PmlI 256 CACGTG CAC^GTG
PpuMI 69 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 177 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 577 CTGCAG CTGCA^G
RsaI 474 GTAC GT^AC
SspBI 473 TGTACA T^GTACA
Van91I 507 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 248 CCAGCGTCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 446 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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BbvCI 405 CCTCAGC CCTCAGC(-5/-2)
732 CCTCAGC
BbvI 551 GCTGC GCAGC(8/12)
685 GCTGC
BpmI 505 CTCCAG CTGGAG(16/14)
341 CTGGAG
BsrI 200 ACTGG ACTGG(1/-1)
647 ACTGG
BstXI 104 CCACAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
640 CCACTCAACTGG
DpnII 432 GATC ^GATC
447 GATC
DrdI 207 GACGGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
757 GACGAGGCTGTC
EaeI 143 CGGCCG [CT]^GGCC[GA]
260 TGGCCA
HphI 481 TCACC GGTGA(8/7)
491 TCACC
MboI 432 GATC ^GATC
447 GATC
MboII 20 GAAGA GAAGA(8/7)
705 TCTTC
NcoI 80 CCATGG C^CATGG
273 CCATGG
NdeII 432 GATC ^GATC
447 GATC
NlaIII 81 CATG CATG^
274 CATG
Sau3AI 432 GATC ^GATC
447 GATC
SfaNI 778 GATGC GCATC(5/9)
418 GCATC
SfcI 441 CTACAG C^T[GA][CT]AG
577 CTGCAG
SmlI 45 CTTGAG C^T[CT][GA]AG
559 CTCAAG
TseI 551 GCTGC G^C[AT]GC
685 GCTGC
Tsp45I 76 GTGAC ^GT[GC]AC
622 GTGAC
TspRI 451 CACTG CA[GC]TG[ACGT][ACGT]^
711 CAGTG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AlwI AvaII BcnI
BsaJI BsiYI BslI BsmAI BsmFI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeIII MnlI
MspI MvaI NciI NlaIV PleI
Sau96I ScrFI StyI