(Circular) (Six-base) MAPSORT of: pFN21AB7944.seq Check: 4518 from: 1 to: 5443 pFN21AB7944 5443 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2244 3253 278 Size: 53 83 186 936 708 1009 2468 Fragments arranged by size: 2468 1009 936 708 186 83 53 AccI GT'mk_AC Cuts at: 1937 2786 1937 Size: 849 4594 Acc65I G'GTAC_C Cuts at: 2764 2764 Size: 5443 AclI AA'CG_TT Cuts at: 1563 3570 3943 1563 Size: 2007 373 3063 Fragments arranged by size: 3063 2007 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3506 4890 5158 1632 Size: 1874 1384 268 1917 Fragments arranged by size: 1917 1874 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5246 AflIII A'CryG_T Cuts at: 1203 4342 5319 1203 Size: 3139 977 1327 Fragments arranged by size: 3139 1327 977 AgeI A'CCGG_T Cuts at: 4274 4274 Size: 5443 AhdI GACnn_n'nnGTC Cuts at: 4172 4172 Size: 5443 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5443 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5443 AlwNI CAG_nnn'CTG Cuts at: 2221 2255 3186 4758 2221 Size: 34 931 1572 2906 Fragments arranged by size: 2906 1572 931 34 ApaI G_GGCC'C Cuts at: 2078 2078 Size: 5443 ApaLI G'TGCA_C Cuts at: 3500 4656 3500 Size: 1156 4287 ApoI r'AATT_y Cuts at: 1493 2752 2978 3130 5239 1493 Size: 1259 226 152 2109 1697 Fragments arranged by size: 2109 1697 1259 226 152 AseI AT'TA_AT Cuts at: 160 1032 3997 160 Size: 872 2965 1606 Fragments arranged by size: 2965 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5443 AvaI C'yCGr_G Cuts at: 1870 1943 2071 2768 1870 Size: 73 128 697 4545 Fragments arranged by size: 4545 697 128 73 BamHI G'GATC_C Cuts at: 2123 2773 3140 2123 Size: 650 367 4426 Fragments arranged by size: 4426 650 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2339 2360 2764 4219 618 Size: 325 217 627 552 21 404 1455 1842 Fragments arranged by size: 1842 1455 627 552 404 325 217 21 BanII G_rGCy'C Cuts at: 1391 2059 2078 2162 2730 2762 1391 Size: 668 19 84 568 32 4072 Fragments arranged by size: 4072 668 568 84 32 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2493 928 Size: 971 594 3878 Fragments arranged by size: 3878 971 594 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2218 2252 3634 3668 1903 Size: 34 281 34 1382 34 3678 Fragments arranged by size: 3678 1382 281 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3335 4545 3335 Size: 1210 4233 BclI T'GATC_A Cuts at: 1548 1548 Size: 5443 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2034 4054 136 Size: 107 122 71 631 605 362 2020 1525 Fragments arranged by size: 2020 1525 631 605 362 122 107 71 BglII A'GATC_T Cuts at: 2706 5438 2706 Size: 2732 2711 BlpI GC'TnA_GC Cuts at: 2860 2860 Size: 5443 Bme1580I G_kGCm'C Cuts at: 1790 2078 2365 3504 4660 1790 Size: 288 287 1139 1156 2573 Fragments arranged by size: 2573 1156 1139 288 287 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5443 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3174 4132 442 Size: 416 53 83 399 24 1757 958 1753 Fragments arranged by size: 1757 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5443 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2176 2176 Size: 5443 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2186 2459 2723 4103 1239 Size: 890 57 273 264 1380 2579 Fragments arranged by size: 2579 1380 890 273 264 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3568 4433 4731 3568 Size: 865 298 4280 Fragments arranged by size: 4280 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4106 882 Size: 617 2607 2219 Fragments arranged by size: 2607 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3234 493 Size: 711 2030 2702 Fragments arranged by size: 2702 2030 711 BsaBI GATnn'nnATC Cuts at: 5437 5437 Size: 5443 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2241 3250 3632 4267 Size: 53 83 186 936 708 1009 382 635 Cuts at: 4267 275 Size: 1451 Fragments arranged by size: 1451 1009 936 708 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2385 3876 4274 4548 4695 5106 2385 Size: 1491 398 274 147 411 2722 Fragments arranged by size: 2722 1491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2579 2609 1178 Size: 30 165 30 1176 30 4012 Fragments arranged by size: 4012 1176 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 2183 2728 1186 Size: 982 15 545 3901 Fragments arranged by size: 3901 982 545 15 BseYI C'CCAG_C Cuts at: 2060 2077 2332 4646 5142 2060 Size: 17 255 2314 496 2361 Fragments arranged by size: 2361 2314 496 255 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2275 1163 Size: 573 539 4331 Fragments arranged by size: 4331 573 539 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2893 3654 4682 1540 Size: 275 186 892 761 1028 2301 Fragments arranged by size: 2301 1028 892 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3504 3589 4660 3504 Size: 85 1071 4287 Fragments arranged by size: 4287 1071 85 BsmI GAATG_Cn' Cuts at: 2954 3047 2954 Size: 93 5350 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2078 2162 2365 2730 2762 3504 Size: 399 269 19 84 203 365 32 742 Cuts at: 3504 3589 4660 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 365 269 203 85 84 32 19 BspEI T'CCGG_A Cuts at: 5106 5106 Size: 5443 BspHI T'CATG_A Cuts at: 3332 5062 3332 Size: 1730 3713 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2652 2798 844 Size: 1808 146 3489 Fragments arranged by size: 3489 1808 146 BsrBI CCG'CTC Cuts at: 1394 1823 2019 3330 1394 Size: 429 196 1311 3507 Fragments arranged by size: 3507 1311 429 196 BsrDI GCAATG_nn' Cuts at: 65 1248 2203 3938 4112 65 Size: 1183 955 1735 174 1396 Fragments arranged by size: 1735 1396 1183 955 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4087 4274 1700 Size: 110 2277 187 2869 Fragments arranged by size: 2869 2277 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5443 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5443 BssSI C'ACGA_G Cuts at: 2593 3503 4515 2593 Size: 910 1012 3521 Fragments arranged by size: 3521 1012 910 BstAPI GCAn_nnn'nTGC Cuts at: 794 2718 794 Size: 1924 3519 BstBI TT'CG_AA Cuts at: 4247 4247 Size: 5443 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5443 BstXI CCAn_nnnn'nTGG Cuts at: 2198 2198 Size: 5443 BstYI r'GATC_y Cuts at: 1972 2123 2466 2706 2773 3140 3525 3542 4994 Size: 151 343 240 67 367 385 17 1452 Cuts at: 4994 5080 5092 5438 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 343 240 151 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 2271 2271 Size: 5443 BtgI C'CryG_G Cuts at: 513 1065 2691 513 Size: 552 1626 3265 Fragments arranged by size: 3265 1626 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4704 Fragments arranged by size: 4704 683 56 BtsI GCAGTG_nn' Cuts at: 2500 2959 3753 3773 2500 Size: 459 794 20 4170 Fragments arranged by size: 4170 794 459 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5443 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5408 DraI TTT'AAA Cuts at: 2747 2747 Size: 5443 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2240 2495 4450 809 Size: 507 924 255 1955 1802 Fragments arranged by size: 1955 1802 924 507 255 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2020 2317 2890 Size: 54 1425 50 275 18 190 297 573 Cuts at: 2890 3779 5408 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 573 297 275 190 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2890 1537 Size: 275 1078 4090 Fragments arranged by size: 4090 1078 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2426 3373 1353 Size: 1073 947 3423 Fragments arranged by size: 3423 1073 947 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4030 4404 4550 387 Size: 1064 138 2441 374 146 1280 Fragments arranged by size: 2441 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2186 2459 2723 3506 4103 4890 Size: 393 497 57 273 264 783 597 787 Cuts at: 4890 5158 1239 Size: 268 1524 Fragments arranged by size: 1524 787 783 597 497 393 273 268 264 57 EcoNI CCTnn'n_nnAGG Cuts at: 2168 2168 Size: 5443 EcoO109I rG'GnC_Cy Cuts at: 2218 2218 Size: 5443 EcoRI G'AATT_C Cuts at: 2752 3130 2752 Size: 378 5065 FspI TGC'GCA Cuts at: 3949 3949 Size: 5443 HaeII r_GCGC'y Cuts at: 1678 2029 4590 1678 Size: 351 2561 2531 Fragments arranged by size: 2561 2531 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4084 4116 4158 4190 1373 Size: 32 135 32 2512 32 42 32 2626 Fragments arranged by size: 2626 2512 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2787 3033 669 Size: 988 281 849 246 3079 Fragments arranged by size: 3079 988 849 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5443 HpaI GTT'AAC Cuts at: 3033 3033 Size: 5443 KpnI G_GTAC'C Cuts at: 2768 2768 Size: 5443 MfeI C'AATT_G Cuts at: 3042 3042 Size: 5443 MluI A'CGCG_T Cuts at: 5319 5319 Size: 5443 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4557 4741 1305 Size: 3252 184 2007 Fragments arranged by size: 3252 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2319 10 Size: 54 1425 343 487 3134 Fragments arranged by size: 3134 1425 487 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3401 3760 3919 518 Size: 648 2235 359 159 2042 Fragments arranged by size: 2235 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2693 2859 3536 4684 4929 1606 Size: 1087 166 677 1148 245 2120 Fragments arranged by size: 2120 1148 1087 677 245 166 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4891 NdeI CA'TA_TG Cuts at: 387 387 Size: 5443 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5443 NotI GC'GGCC_GC Cuts at: 2890 2890 Size: 5443 NspI r_CATG'y Cuts at: 1132 1229 2265 2801 4346 1132 Size: 97 1036 536 1545 2229 Fragments arranged by size: 2229 1545 1036 536 97 PasI CC'CwG_GG Cuts at: 1348 2215 2365 1348 Size: 867 150 4426 Fragments arranged by size: 4426 867 150 PciI A'CATG_T Cuts at: 4342 4342 Size: 5443 PflMI CCAn_nnn'nTGG Cuts at: 1108 2221 1108 Size: 1113 4330 PfoI T'CCnGG_A Cuts at: 2553 2670 2553 Size: 117 5326 PmeI GTTT'AAAC Cuts at: 2747 2747 Size: 5443 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3512 3544 5025 5057 3512 Size: 32 1481 32 3898 Fragments arranged by size: 3898 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5443 PsiI TTA'TAA Cuts at: 3013 3013 Size: 5443 PspOMI G'GGCC_C Cuts at: 2074 2074 Size: 5443 PstI C_TGCA'G Cuts at: 830 2582 2795 830 Size: 1752 213 3478 Fragments arranged by size: 3478 1752 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5443 SacII CC_GC'GG Cuts at: 2694 2694 Size: 5443 SalI G'TCGA_C Cuts at: 1936 2785 1936 Size: 849 4594 SbfI CC_TGCA'GG Cuts at: 2795 2795 Size: 5443 ScaI AGT'ACT Cuts at: 3691 5403 3691 Size: 1712 3731 SexAI A'CCwGG_T Cuts at: 2010 2010 Size: 5443 SfcI C'TryA_G Cuts at: 826 1045 2578 2791 3926 4607 4798 826 Size: 219 1533 213 1135 681 191 1471 Fragments arranged by size: 1533 1471 1135 681 219 213 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5443 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5443 SmaI CCC'GGG Cuts at: 1872 2073 2770 1872 Size: 201 697 4545 Fragments arranged by size: 4545 697 201 SmlI C'TyrA_G Cuts at: 820 1017 1943 3547 4448 4710 820 Size: 197 926 1604 901 262 1553 Fragments arranged by size: 1604 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3234 493 Size: 2741 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5443 SphI G_CATG'C Cuts at: 1132 2801 1132 Size: 1669 3774 SspI AAT'ATT Cuts at: 5 52 3367 5 Size: 47 3315 2081 Fragments arranged by size: 3315 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5443 StyI C'CwwG_G Cuts at: 513 1065 2191 2283 2376 2882 513 Size: 552 1126 92 93 506 3074 Fragments arranged by size: 3074 1126 552 506 93 92 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2357 2418 3470 3487 3640 3825 4270 Size: 1255 777 61 1052 17 153 185 445 Cuts at: 4270 325 Size: 1498 Fragments arranged by size: 1498 1255 1052 777 445 185 153 61 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3689 5401 96 Size: 274 80 33 51 1443 1712 1712 138 Fragments arranged by size: 1712 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2579 2611 5403 5435 2579 Size: 32 2792 32 2587 Fragments arranged by size: 2792 2587 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5443 XbaI T'CTAG_A Cuts at: 2779 2779 Size: 5443 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2030 2030 Size: 5443 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5443 XmaI C'CCGG_G Cuts at: 1870 2071 2768 1870 Size: 201 697 4545 Fragments arranged by size: 4545 697 201 XmnI GAAnn'nnTTC Cuts at: 3572 3572 Size: 5443 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2242 3251 276 Size: 53 83 186 936 708 1009 2468 Fragments arranged by size: 2468 1009 936 708 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI