Restriction map for
pFN21AB7937
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BamHI BbeI BbrPI
BcgI BclI BcnI BfaI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 772 CCGC CCGC(-3/-1)
AlwNI 610 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 64 ATTAAT AT^TAAT
AsnI 64 ATTAAT AT^TAAT
BalI 759 TGGCCA TGG^CCA
BanI 511 GGTGCC G^G[CT][GA]CC
BanII 934 GAGCCC G[GA]GC[CT]^C
BciVI 831 GGATAC GTATCC(6/5)
BglII 43 AGATCT A^GATCT
BlpI 268 GCTCAGC GC^T[ACGT]AGC
BpmI 629 CTGGAG CTGGAG(16/14)
Bpu1102I 268 GCTCAGC GC^T[ACGT]AGC
BsaJI 158 CCAGGG C^C[ACGT][ACGT]GG
BsmAI 77 GAGAC GTCTC(1/5)
BsmI 387 GAATGC GAATGC(1/-1)
BstAPI 430 GCACTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 43 AGATCT [GA]^GATC[CT]
CelII 268 GCTCAGC GC^T[ACGT]AGC
CfoI 298 GCGC GCG^C
Cfr13I 777 GGCCC G^G[ACGT]CC
DpnII 44 GATC ^GATC
EaeI 759 TGGCCA [CT]^GGCC[GA]
EcoT22I 389 ATGCAT ATGCA^T
HhaI 298 GCGC GCG^C
HinP1I 298 GCGC G^CGC
MaeIII 445 GTGAC ^GT[ACGT]AC
MboI 44 GATC ^GATC
MflI 43 AGATCT [GA]^GATC[CT]
MluNI 759 TGGCCA TGG^CCA
MscI 759 TGGCCA TGG^CCA
MslI 382 CATATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 416 CAGCTG C[AC]G^C[GT]G
NdeI 382 CATATG CA^TATG
NdeII 44 GATC ^GATC
NlaIII 399 CATG CATG^
NsiI 389 ATGCAT ATGCA^T
PshAI 648 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 64 ATTAAT AT^TAAT
PstI 437 CTGCAG CTGCA^G
PvuII 416 CAGCTG CAG^CTG
Sau3AI 44 GATC ^GATC
Sau96I 777 GGCCC G^G[ACGT]CC
SbfI 436 CCTGCAGG CCTGCA^GG
ScaI 111 AGTACT AGT^ACT
Sse8387I 436 CCTGCAGG CCTGCA^GG
Tsp45I 445 GTGAC ^GT[GC]AC
XhoII 43 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 471 AAATTC [GA]^AATT[CT]
1048 AAATTT
ApoI 471 AAATTC [GA]^AATT[CT]
1048 AAATTT
BbsI 865 GAAGAC GAAGAC(2/6)
889 GAAGAC
BmyI 512 GTGCCC G[AGT]GC[ACT]^C
934 GAGCCC
BpuAI 865 GAAGAC GAAGAC(2/6)
889 GAAGAC
BseRI 620 CTCCTC GAGGAG(10/8)
883 GAGGAG
BsiYI 622 CCTCTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
643 CCACAGACAGG
BslI 622 CCTCTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
643 CCACAGACAGG
Bsp1286I 512 GTGCCC G[AGT]GC[ACT]^C
934 GAGCCC
BsrI 757 ACTGG ACTGG(1/-1)
826 ACTGG
NlaIV 511 GGTGCC GG[ACGT]^[ACGT]CC
618 GGCTCC
PleI 447 GACTC GAGTC(4/5)
696 GACTC
SapI 184 GAAGAGC GCTCTTC(1/4)
931 GAAGAGC
SfcI 413 CTACAG C^T[GA][CT]AG
437 CTGCAG
SmlI 698 CTCAAG C^T[CT][GA]AG
1057 CTCAAG
TfiI 364 GATTC G^A[AT]TC
1074 GAATC
TspRI 542 CAGTG CA[GC]TG[ACGT][ACGT]^
716 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI AluI BbvCI BbvI BspMI
BssKI BstF5I BstNI Cac8I DdeI
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MboII
MnlI MseI MvaI MwoI RsaI
ScrFI SfaNI Tru9I TseI Tsp509I