Restriction map for
pFN21AB7935
(Circular) (Six-base) MAPSORT of: pFN21AB7935.seq Check: 6042 from: 1 to: 5818
pFN21AB7935 5818 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2592 3628 278
Size: 53 83 186 936 1056 1036 2468
Fragments arranged by size:
2468 1056 1036 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3057 3161 1937
Size: 1120 104 4594
Fragments arranged by size:
4594 1120 104
Acc65I G'GTAC_C
Cuts at: 3139 3139
Size: 5818
AclI AA'CG_TT
Cuts at: 1563 3117 3945 4318 1563
Size: 1554 828 373 3063
Fragments arranged by size:
3063 1554 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2032 3881 5265 5533 1632
Size: 400 1849 1384 268 1917
Fragments arranged by size:
1917 1849 1384 400 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5621
AflIII A'CryG_T
Cuts at: 1203 4717 5694 1203
Size: 3514 977 1327
Fragments arranged by size:
3514 1327 977
AgeI A'CCGG_T
Cuts at: 4649 4649
Size: 5818
AhdI GACnn_n'nnGTC
Cuts at: 4547 4547
Size: 5818
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5818
AlwNI CAG_nnn'CTG
Cuts at: 2221 2509 2606 3561 5133 2221
Size: 288 97 955 1572 2906
Fragments arranged by size:
2906 1572 955 288 97
ApaI G_GGCC'C
Cuts at: 2478 2478
Size: 5818
ApaLI G'TGCA_C
Cuts at: 3875 5031 3875
Size: 1156 4662
ApoI r'AATT_y
Cuts at: 1493 3127 3353 3505 5614 1493
Size: 1634 226 152 2109 1697
Fragments arranged by size:
2109 1697 1634 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4372 160
Size: 872 3340 1606
Fragments arranged by size:
3340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
AvaI C'yCGr_G
Cuts at: 1870 1943 2786 3143 1870
Size: 73 843 357 4545
Fragments arranged by size:
4545 843 357 73
BamHI G'GATC_C
Cuts at: 2662 2719 3148 3515 2662
Size: 57 429 367 4965
Fragments arranged by size:
4965 429 367 57
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 2819 3139 4594 618
Size: 325 217 627 513 519 320 1455 1842
Fragments arranged by size:
1842 1455 627 519 513 325 320 217
BanII G_rGCy'C
Cuts at: 1391 2412 2478 2569 2624 2868 3137 1391
Size: 1021 66 91 55 244 269 4072
Fragments arranged by size:
4072 1021 269 244 91 66 55
BbeI G_GCGC'C
Cuts at: 2304 2304
Size: 5818
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 2902 928
Size: 971 317 686 3844
Fragments arranged by size:
3844 971 686 317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4009 4043 1903
Size: 34 2072 34 3678
Fragments arranged by size:
3678 2072 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3710 4920 3710
Size: 1210 4608
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5818
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4429 136
Size: 107 122 71 631 605 2757 1525
Fragments arranged by size:
2757 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5813 5813
Size: 5818
BlpI GC'TnA_GC
Cuts at: 2762 2990 3235 2762
Size: 228 245 5345
Fragments arranged by size:
5345 245 228
Bme1580I G_kGCm'C
Cuts at: 1790 2323 2478 3879 5035 1790
Size: 533 155 1401 1156 2573
Fragments arranged by size:
2573 1401 1156 533 155
BmgBI CAC'GTC
Cuts at: 1318 2653 1318
Size: 1335 4483
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2191 2785 3549
Size: 416 53 83 399 24 774 594 764
Cuts at: 3549 4507 442
Size: 958 1753
Fragments arranged by size:
1753 958 774 764 594 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 3089 4478 1239
Size: 1214 636 1389 2579
Fragments arranged by size:
2579 1389 1214 636
Bpu10I CC'TnA_GC
Cuts at: 2135 2135
Size: 5818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 3061 3943 4808 5106 2345
Size: 716 882 865 298 3057
Fragments arranged by size:
3057 882 865 716 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2376 4481 882
Size: 617 877 2105 2219
Fragments arranged by size:
2219 2105 877 617
BsaAI yAC'GTr
Cuts at: 493 1204 3609 493
Size: 711 2405 2702
Fragments arranged by size:
2702 2405 711
BsaBI GATnn'nnATC
Cuts at: 5812 5812
Size: 5818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2301 2589 2907 3625
Size: 53 83 186 936 768 288 318 718
Cuts at: 3625 4007 4642 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 768 718 635 382 318 288
186 83 53
BsaWI w'CCGG_w
Cuts at: 2639 4251 4649 4923 5070 5481 2639
Size: 1612 398 274 147 411 2976
Fragments arranged by size:
2976 1612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5593
Fragments arranged by size:
5593 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 2620 1186
Size: 1156 278 4384
Fragments arranged by size:
4384 1156 278
BseYI C'CCAG_C
Cuts at: 2373 2687 5021 5517 2373
Size: 314 2334 496 2674
Fragments arranged by size:
2674 2334 496 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5245
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3268 4029 5057 1540
Size: 275 186 1267 761 1028 2301
Fragments arranged by size:
2301 1267 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2412 2624 2868 3879 3964 5035 2412
Size: 212 244 1011 85 1071 3195
Fragments arranged by size:
3195 1071 1011 244 212 85
BsmI GAATG_Cn'
Cuts at: 3329 3422 3329
Size: 93 5725
BsmBI CGTCTCn'nnnn_
Cuts at: 2596 2596
Size: 5818
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2323 2412 2478 2569 2624 2868 3137
Size: 399 533 89 66 91 55 244 269
Cuts at: 3137 3879 3964 5035 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 533 399 269 244 91
89 85 66 55
BspEI T'CCGG_A
Cuts at: 5481 5481
Size: 5818
BspHI T'CATG_A
Cuts at: 2880 3707 5437 2880
Size: 827 1730 3261
Fragments arranged by size:
3261 1730 827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2206 3173 844
Size: 1362 967 3489
Fragments arranged by size:
3489 1362 967
BsrBI CCG'CTC
Cuts at: 1394 1823 3705 1394
Size: 429 1882 3507
Fragments arranged by size:
3507 1882 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4313 4487 65
Size: 1183 3065 174 1396
Fragments arranged by size:
3065 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2748 3045 4462 4649 1700
Size: 110 938 297 1417 187 2869
Fragments arranged by size:
2869 1417 938 297 187 110
BsrGI T'GTAC_A
Cuts at: 96 2058 96
Size: 1962 3856
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5818
BssSI C'ACGA_G
Cuts at: 3878 4890 3878
Size: 1012 4806
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5818
BstBI TT'CG_AA
Cuts at: 4622 4622
Size: 5818
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5818
BstXI CCAn_nnnn'nTGG
Cuts at: 2182 2182
Size: 5818
BstYI r'GATC_y
Cuts at: 1972 2662 2719 3024 3148 3515 3900 3917 5369
Size: 690 57 305 124 367 385 17 1452
Cuts at: 5369 5455 5467 5813 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 690 385 367 346 305 124
86 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4327
Fragments arranged by size:
4327 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2069 2706 487
Size: 683 56 843 637 3599
Fragments arranged by size:
3599 843 683 637 56
BtsI GCAGTG_nn'
Cuts at: 3334 4128 4148 3334
Size: 794 20 5004
Fragments arranged by size:
5004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5783
DraI TTT'AAA
Cuts at: 3122 3122
Size: 5818
DraIII CAC_nnn'GTG
Cuts at: 2288 2288
Size: 5818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4825 809
Size: 507 3509 1802
Fragments arranged by size:
3509 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3043 3265 4154
Size: 54 1425 50 275 18 1213 222 889
Cuts at: 4154 5783 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1213 889 275 222 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3265 1537
Size: 275 1453 4090
Fragments arranged by size:
4090 1453 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2404 2687 2792 3090 3748 1353
Size: 1051 283 105 298 658 3423
Fragments arranged by size:
3423 1051 658 298 283 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4405 4779 4925 387
Size: 1064 138 2816 374 146 1280
Fragments arranged by size:
2816 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2410 2622 2866 2410
Size: 212 244 5362
Fragments arranged by size:
5362 244 212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2453 3089 3881 4478 5265 5533
Size: 393 400 421 636 792 597 787 268
Cuts at: 5533 1239
Size: 1524
Fragments arranged by size:
1524 792 787 636 597 421 400 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2461 2474 2475 2875 2461
Size: 13 1 400 5404
Fragments arranged by size:
5404 400 13 1
EcoRI G'AATT_C
Cuts at: 3127 3505 3127
Size: 378 5440
FseI GG_CCGG'CC
Cuts at: 2752 2752
Size: 5818
FspI TGC'GCA
Cuts at: 4324 4324
Size: 5818
HaeII r_GCGC'y
Cuts at: 1678 2271 2304 4965 1678
Size: 593 33 2661 2531
Fragments arranged by size:
2661 2531 593 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4459 4491 4533 4565 1373
Size: 32 135 32 2887 32 42 32 2626
Fragments arranged by size:
2887 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3162 3408 669
Size: 988 281 1224 246 3079
Fragments arranged by size:
3079 1224 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5818
HpaI GTT'AAC
Cuts at: 3408 3408
Size: 5818
KasI G'GCGC_C
Cuts at: 2300 2300
Size: 5818
KpnI G_GTAC'C
Cuts at: 3143 3143
Size: 5818
MfeI C'AATT_G
Cuts at: 2314 3417 2314
Size: 1103 4715
MluI A'CGCG_T
Cuts at: 5694 5694
Size: 5818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2396 3092 4932 5116 1305
Size: 1091 696 1840 184 2007
Fragments arranged by size:
2007 1840 1091 696 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3996
Fragments arranged by size:
3996 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3776 4135 4294 518
Size: 648 2610 359 159 2042
Fragments arranged by size:
2610 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2178 2377 2553 3234 3911 5059 5304 1606
Size: 572 199 176 681 677 1148 245 2120
Fragments arranged by size:
2120 1148 681 677 572 245 199 176
NaeI GCC'GGC
Cuts at: 2750 3047 2750
Size: 297 5521
NarI GG'CG_CC
Cuts at: 2301 2301
Size: 5818
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4327
Fragments arranged by size:
4327 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5818
NgoMIV G'CCGG_C
Cuts at: 2748 3045 2748
Size: 297 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5818
NotI GC'GGCC_GC
Cuts at: 3265 3265
Size: 5818
NspI r_CATG'y
Cuts at: 1132 1229 3176 4721 1132
Size: 97 1947 1545 2229
Fragments arranged by size:
2229 1947 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2471 1348
Size: 1123 4695
PciI A'CATG_T
Cuts at: 4717 4717
Size: 5818
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5818
PfoI T'CCnGG_A
Cuts at: 2454 2841 3027 2454
Size: 387 186 5245
Fragments arranged by size:
5245 387 186
PmeI GTTT'AAAC
Cuts at: 3122 3122
Size: 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 5400 5432 3887
Size: 32 1481 32 4273
Fragments arranged by size:
4273 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2520 1613
Size: 907 4911
PsiI TTA'TAA
Cuts at: 3388 3388
Size: 5818
PspOMI G'GGCC_C
Cuts at: 2474 2474
Size: 5818
PstI C_TGCA'G
Cuts at: 830 3170 830
Size: 2340 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5818
PvuII CAG'CTG
Cuts at: 2178 2377 2553 2178
Size: 199 176 5443
Fragments arranged by size:
5443 199 176
SacI G_AGCT'C
Cuts at: 2412 2624 2868 2412
Size: 212 244 5362
Fragments arranged by size:
5362 244 212
SalI G'TCGA_C
Cuts at: 1936 3160 1936
Size: 1224 4594
SapI GCTCTTCn'nnn_
Cuts at: 2404 2687 2792 2404
Size: 283 105 5430
Fragments arranged by size:
5430 283 105
SbfI CC_TGCA'GG
Cuts at: 3170 3170
Size: 5818
ScaI AGT'ACT
Cuts at: 2774 4066 5778 2774
Size: 1292 1712 2814
Fragments arranged by size:
2814 1712 1292
SfcI C'TryA_G
Cuts at: 826 1045 3166 4301 4982 5173 826
Size: 219 2121 1135 681 191 1471
Fragments arranged by size:
2121 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2302 2302
Size: 5818
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5818
SmaI CCC'GGG
Cuts at: 1872 3145 1872
Size: 1273 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2324 2786 3076 3922 4823 5085
Size: 197 926 381 462 290 846 901 262
Cuts at: 5085 820
Size: 1553
Fragments arranged by size:
1553 926 901 846 462 381 290 262
197
SnaBI TAC'GTA
Cuts at: 493 3609 493
Size: 3116 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5818
SphI G_CATG'C
Cuts at: 1132 3176 1132
Size: 2044 3774
SspI AAT'ATT
Cuts at: 5 52 3742 5
Size: 47 3690 2081
Fragments arranged by size:
3690 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5818
StyI C'CwwG_G
Cuts at: 513 1065 2004 3257 513
Size: 552 939 1253 3074
Fragments arranged by size:
3074 1253 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2837 3845 3862 4015 4200 4645 325
Size: 1255 1257 1008 17 153 185 445 1498
Fragments arranged by size:
1498 1257 1255 1008 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 2772 4064
Size: 274 80 33 51 1443 81 714 1292
Cuts at: 4064 5776 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1292 714 274 138 81 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2637 2669 5778 5810 2637
Size: 32 3109 32 2645
Fragments arranged by size:
3109 2645 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5818
XbaI T'CTAG_A
Cuts at: 3154 3154
Size: 5818
XhoI C'TCGA_G
Cuts at: 1943 2786 1943
Size: 843 4975
XmaI C'CCGG_G
Cuts at: 1870 3143 1870
Size: 1273 4545
XmnI GAAnn'nnTTC
Cuts at: 3099 3947 3099
Size: 848 4970
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2590 3626 276
Size: 53 83 186 936 1056 1036 2468
Fragments arranged by size:
2468 1056 1036 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FspAI
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SrfI SwaI XcmI