(Circular) (Six-base) MAPSORT of: pFN21AB7929.seq Check: 4819 from: 1 to: 5761 pFN21AB7929 5761 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2341 2341 Size: 5761 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2383 3571 278 Size: 53 83 186 936 847 1188 2468 Fragments arranged by size: 2468 1188 936 847 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 3082 3082 Size: 5761 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2523 2838 3824 5208 5476 1632 Size: 891 315 986 1384 268 1917 Fragments arranged by size: 1917 1384 986 891 315 268 AflII C'TTAA_G Cuts at: 820 1017 2387 820 Size: 197 1370 4194 Fragments arranged by size: 4194 1370 197 AflIII A'CryG_T Cuts at: 1203 2701 2910 4660 5637 1203 Size: 1498 209 1750 977 1327 Fragments arranged by size: 1750 1498 1327 977 209 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5761 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AlwNI CAG_nnn'CTG Cuts at: 2236 2816 3504 5076 2236 Size: 580 688 1572 2921 Fragments arranged by size: 2921 1572 688 580 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 2587 2777 3070 3296 3448 5557 1493 Size: 1094 190 293 226 152 2109 1697 Fragments arranged by size: 2109 1697 1094 293 226 190 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 2126 2755 3086 1870 Size: 73 183 629 331 4545 Fragments arranged by size: 4545 629 331 183 73 BamHI G'GATC_C Cuts at: 2858 3091 3458 2858 Size: 233 367 5161 Fragments arranged by size: 5161 367 233 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2192 2644 3082 4537 Size: 325 217 627 273 132 452 438 1455 Cuts at: 4537 618 Size: 1842 Fragments arranged by size: 1842 1455 627 452 438 325 273 217 132 BanII G_rGCy'C Cuts at: 1391 3080 1391 Size: 1689 4072 BbeI G_GCGC'C Cuts at: 2064 2196 2064 Size: 132 5629 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2011 928 Size: 971 112 4678 Fragments arranged by size: 4678 971 112 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 1548 Size: 5761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4372 136 Size: 107 122 71 631 605 2700 1525 Fragments arranged by size: 2700 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5756 5756 Size: 5761 BlpI GC'TnA_GC Cuts at: 2030 2474 3178 2030 Size: 444 704 4613 Fragments arranged by size: 4613 704 444 Bme1580I G_kGCm'C Cuts at: 1790 3822 4978 1790 Size: 2032 1156 2573 Fragments arranged by size: 2573 2032 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2552 3492 4450 Size: 416 53 83 399 24 1135 940 958 Cuts at: 4450 442 Size: 1753 Fragments arranged by size: 1753 1135 958 940 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4421 1239 Size: 3182 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2141 2243 3886 4751 5049 2141 Size: 102 1643 865 298 2853 Fragments arranged by size: 2853 1643 865 298 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 882 Size: 617 2925 2219 Fragments arranged by size: 2925 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2641 2913 3552 493 Size: 711 1437 272 639 2702 Fragments arranged by size: 2702 1437 711 639 272 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2193 2380 3568 Size: 53 83 186 936 528 132 187 1188 Cuts at: 3568 3950 4585 275 Size: 382 635 1451 Fragments arranged by size: 1451 1188 936 635 528 382 187 186 132 83 53 BsaWI w'CCGG_w Cuts at: 2181 4194 4592 4866 5013 5424 2181 Size: 2013 398 274 147 411 2518 Fragments arranged by size: 2518 2013 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5536 Fragments arranged by size: 5536 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5761 BseYI C'CCAG_C Cuts at: 2418 4964 5460 2418 Size: 2546 496 2719 Fragments arranged by size: 2719 2546 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 3071 1163 Size: 573 633 702 3853 Fragments arranged by size: 3853 702 633 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3822 3907 4978 3822 Size: 85 1071 4605 Fragments arranged by size: 4605 1071 85 BsmI GAATG_Cn' Cuts at: 2136 3272 3365 2136 Size: 1136 93 4532 Fragments arranged by size: 4532 1136 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3080 3822 3907 4978 1391 Size: 399 1290 742 85 1071 2174 Fragments arranged by size: 2174 1290 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2341 3116 844 Size: 1497 775 3489 Fragments arranged by size: 3489 1497 775 BsrBI CCG'CTC Cuts at: 1394 1823 2071 2077 3648 1394 Size: 429 248 6 1571 3507 Fragments arranged by size: 3507 1571 429 248 6 BsrDI GCAATG_nn' Cuts at: 65 1248 2467 4256 4430 65 Size: 1183 1219 1789 174 1396 Fragments arranged by size: 1789 1396 1219 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 2189 4405 4592 1700 Size: 110 275 104 2216 187 2869 Fragments arranged by size: 2869 2216 275 187 110 104 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 2236 794 Size: 1442 4319 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstYI r'GATC_y Cuts at: 1972 2759 2858 3091 3458 3843 3860 5312 5398 Size: 787 99 233 367 385 17 1452 86 Cuts at: 5398 5410 5756 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 787 385 367 346 233 99 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5209 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 2270 2491 3277 4071 4091 2270 Size: 221 786 794 20 3940 Fragments arranged by size: 3940 794 786 221 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 3065 3065 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2709 3208 Size: 54 1425 50 275 18 253 626 499 Cuts at: 3208 4097 5726 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 626 499 275 253 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3691 1353 Size: 2338 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4348 4722 4868 387 Size: 1064 138 2759 374 146 1280 Fragments arranged by size: 2759 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2523 2838 3824 4421 5208 5476 1239 Size: 393 891 315 986 597 787 268 1524 Fragments arranged by size: 1524 986 891 787 597 393 315 268 EcoO109I rG'GnC_Cy Cuts at: 2233 2233 Size: 5761 EcoRI G'AATT_C Cuts at: 2587 3070 3448 2587 Size: 483 378 4900 Fragments arranged by size: 4900 483 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2335 2439 2683 2335 Size: 104 244 5413 Fragments arranged by size: 5413 244 104 FseI GG_CCGG'CC Cuts at: 2089 2089 Size: 5761 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 2064 2196 4908 1678 Size: 386 132 2712 2531 Fragments arranged by size: 2712 2531 386 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4402 4434 4476 4508 1373 Size: 32 135 32 2830 32 42 32 2626 Fragments arranged by size: 2830 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3105 3351 669 Size: 988 281 1167 246 3079 Fragments arranged by size: 3079 1167 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KasI G'GCGC_C Cuts at: 2060 2192 2060 Size: 132 5629 KpnI G_GTAC'C Cuts at: 3086 3086 Size: 5761 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4875 5059 1305 Size: 3570 184 2007 Fragments arranged by size: 3570 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2711 10 Size: 54 1425 343 879 3060 Fragments arranged by size: 3060 1425 879 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2723 3719 4078 4237 518 Size: 648 1557 996 359 159 2042 Fragments arranged by size: 2042 1557 996 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2034 2097 2991 3046 3177 3854 5002 5247 Size: 428 63 894 55 131 677 1148 245 Cuts at: 5247 1606 Size: 2120 Fragments arranged by size: 2120 1148 894 677 428 245 131 63 55 NaeI GCC'GGC Cuts at: 2087 2087 Size: 5761 NarI GG'CG_CC Cuts at: 2061 2193 2061 Size: 132 5629 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5209 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NgoMIV G'CCGG_C Cuts at: 2085 2085 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 2705 3119 4664 1132 Size: 97 1476 414 1545 2229 Fragments arranged by size: 2229 1545 1476 414 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 2701 4660 2701 Size: 1959 3802 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5761 PfoI T'CCnGG_A Cuts at: 2103 2103 Size: 5761 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PstI C_TGCA'G Cuts at: 830 2979 3113 830 Size: 2149 134 3478 Fragments arranged by size: 3478 2149 134 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2991 2991 Size: 5761 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SfcI C'TryA_G Cuts at: 826 1045 2092 2975 3109 4244 4925 5116 826 Size: 219 1047 883 134 1135 681 191 1471 Fragments arranged by size: 1471 1135 1047 883 681 219 191 134 SfoI GGC'GCC Cuts at: 2062 2194 2062 Size: 132 5629 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 3088 1872 Size: 1216 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2156 2258 2387 3865 4766 5028 Size: 197 926 213 102 129 1478 901 262 Cuts at: 5028 820 Size: 1553 Fragments arranged by size: 1553 1478 926 901 262 213 197 129 102 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 3200 513 Size: 552 2135 3074 Fragments arranged by size: 3074 2135 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 2581 3788 3805 3958 4143 4588 Size: 1255 741 260 1207 17 153 185 445 Cuts at: 4588 325 Size: 1498 Fragments arranged by size: 1498 1255 1207 741 445 260 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4007 5719 96 Size: 274 80 33 51 1443 2030 1712 138 Fragments arranged by size: 2030 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5721 5753 5721 Size: 32 5729 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 3097 3097 Size: 5761 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2415 2415 Size: 5761 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 3086 1870 Size: 1216 4545 XmnI GAAnn'nnTTC Cuts at: 3890 3890 Size: 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2381 3569 276 Size: 53 83 186 936 847 1188 2468 Fragments arranged by size: 2468 1188 936 847 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI