Restriction map for
pFN21AB7929
(Linear) (Six-base) MAPSORT of: KIBB7929.seq Check: 5956 from: 1 to: 1056
KIBB7929.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 336 1056
Size: 336 720
AatII G_ACGT'C
Cuts at: 0 378 1056
Size: 378 678
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 833 1056
Size: 518 315 223
Fragments arranged by size:
518 315 223
AflII C'TTAA_G
Cuts at: 0 382 1056
Size: 382 674
AflIII A'CryG_T
Cuts at: 0 696 905 1056
Size: 696 209 151
Fragments arranged by size:
696 209 151
AlwNI CAG_nnn'CTG
Cuts at: 0 231 811 1056
Size: 231 580 245
Fragments arranged by size:
580 245 231
ApoI r'AATT_y
Cuts at: 0 582 772 1056
Size: 582 190 284
Fragments arranged by size:
582 284 190
AvaI C'yCGr_G
Cuts at: 0 121 750 1056
Size: 121 629 306
Fragments arranged by size:
629 306 121
BamHI G'GATC_C
Cuts at: 0 853 1056
Size: 853 203
BanI G'GyrC_C
Cuts at: 0 55 187 639 1056
Size: 55 132 452 417
Fragments arranged by size:
452 417 132 55
BbeI G_GCGC'C
Cuts at: 0 59 191 1056
Size: 59 132 865
Fragments arranged by size:
865 132 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 6 1056
Size: 6 1050
BlpI GC'TnA_GC
Cuts at: 0 25 469 1056
Size: 25 444 587
Fragments arranged by size:
587 444 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 547 1056
Size: 547 509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 238 1056
Size: 136 102 818
Fragments arranged by size:
818 136 102
BsaAI yAC'GTr
Cuts at: 0 636 908 1056
Size: 636 272 148
Fragments arranged by size:
636 272 148
BsaHI Gr'CG_yC
Cuts at: 0 56 188 375 1056
Size: 56 132 187 681
Fragments arranged by size:
681 187 132 56
BsaWI w'CCGG_w
Cuts at: 0 176 1056
Size: 176 880
BseYI C'CCAG_C
Cuts at: 0 413 1056
Size: 413 643
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1056
Size: 364 692
BsmI GAATG_Cn'
Cuts at: 0 131 1056
Size: 131 925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 336 1056
Size: 336 720
BsrBI CCG'CTC
Cuts at: 0 66 72 1056
Size: 66 6 984
Fragments arranged by size:
984 66 6
BsrDI GCAATG_nn'
Cuts at: 0 462 1056
Size: 462 594
BsrFI r'CCGG_y
Cuts at: 0 80 184 1056
Size: 80 104 872
Fragments arranged by size:
872 104 80
BstAPI GCAn_nnn'nTGC
Cuts at: 0 231 1056
Size: 231 825
BstYI r'GATC_y
Cuts at: 0 754 853 1056
Size: 754 99 203
Fragments arranged by size:
754 203 99
BtsI GCAGTG_nn'
Cuts at: 0 265 486 1056
Size: 265 221 570
Fragments arranged by size:
570 265 221
EaeI y'GGCC_r
Cuts at: 0 78 704 1056
Size: 78 626 352
Fragments arranged by size:
626 352 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 833 1056
Size: 518 315 223
Fragments arranged by size:
518 315 223
EcoO109I rG'GnC_Cy
Cuts at: 0 228 1056
Size: 228 828
EcoRI G'AATT_C
Cuts at: 0 582 1056
Size: 582 474
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 330 434 678 1056
Size: 330 104 244 378
Fragments arranged by size:
378 330 244 104
FseI GG_CCGG'CC
Cuts at: 0 84 1056
Size: 84 972
HaeII r_GCGC'y
Cuts at: 0 59 191 1056
Size: 59 132 865
Fragments arranged by size:
865 132 59
KasI G'GCGC_C
Cuts at: 0 55 187 1056
Size: 55 132 869
Fragments arranged by size:
869 132 55
MscI TGG'CCA
Cuts at: 0 706 1056
Size: 706 350
MslI CAynn'nnrTG
Cuts at: 0 718 1056
Size: 718 338
MspA1I CmG'CkG
Cuts at: 0 29 92 986 1041 1056
Size: 29 63 894 55 15
Fragments arranged by size:
894 63 55 29 15
NaeI GCC'GGC
Cuts at: 0 82 1056
Size: 82 974
NarI GG'CG_CC
Cuts at: 0 56 188 1056
Size: 56 132 868
Fragments arranged by size:
868 132 56
NgoMIV G'CCGG_C
Cuts at: 0 80 1056
Size: 80 976
NspI r_CATG'y
Cuts at: 0 700 1056
Size: 700 356
PciI A'CATG_T
Cuts at: 0 696 1056
Size: 696 360
PfoI T'CCnGG_A
Cuts at: 0 98 1056
Size: 98 958
PstI C_TGCA'G
Cuts at: 0 974 1056
Size: 974 82
PvuII CAG'CTG
Cuts at: 0 986 1056
Size: 986 70
SfcI C'TryA_G
Cuts at: 0 87 970 1056
Size: 87 883 86
Fragments arranged by size:
883 87 86
SfoI GGC'GCC
Cuts at: 0 57 189 1056
Size: 57 132 867
Fragments arranged by size:
867 132 57
SmlI C'TyrA_G
Cuts at: 0 151 253 382 1056
Size: 151 102 129 674
Fragments arranged by size:
674 151 129 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 576 1056
Size: 316 260 480
Fragments arranged by size:
480 316 260
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 410 1056
Size: 410 646
ZraI GAC'GTC
Cuts at: 0 376 1056
Size: 376 680
Enzymes that do cut:
AarI AatII AcuI AflII AflIII AlwNI ApoI AvaI
BamHI BanI BbeI BbsI BlpI BmrI BpuEI BsaAI
BsaHI BsaWI BseYI BsgI BsmI BspMI BsrBI BsrDI
BsrFI BstAPI BstYI BtsI EaeI Eco57MI EcoO109I EcoRI
FalI FseI HaeII KasI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PstI PvuII SfcI
SfoI SmlI TaqII XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanII BbvCI BcgI BciVI BclI BfrBI BglI BglII
Bme1580I BmgBI BmtI BplI BpmI Bpu10I BsaI BsaBI
BsaXI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRV FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI