(Circular) (Six-base) MAPSORT of: pFN21AB6284.seq Check: 2481 from: 1 to: 7054 pFN21AB6284 7054 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4864 278 Size: 53 83 186 936 3328 2468 Fragments arranged by size: 3328 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4397 1937 Size: 2460 4594 Acc65I G'GTAC_C Cuts at: 2745 3372 4375 2745 Size: 627 1003 5424 Fragments arranged by size: 5424 1003 627 AclI AA'CG_TT Cuts at: 1563 5181 5554 1563 Size: 3618 373 3063 Fragments arranged by size: 3618 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2588 2684 5117 6501 6769 1632 Size: 956 96 2433 1384 268 1917 Fragments arranged by size: 2433 1917 1384 956 268 96 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6857 AflIII A'CryG_T Cuts at: 1203 5953 6930 1203 Size: 4750 977 1327 Fragments arranged by size: 4750 1327 977 AgeI A'CCGG_T Cuts at: 5885 5885 Size: 7054 AhdI GACnn_n'nnGTC Cuts at: 5783 5783 Size: 7054 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7054 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2049 3255 3306 1184 Size: 865 1206 51 4932 Fragments arranged by size: 4932 1206 865 51 AlwNI CAG_nnn'CTG Cuts at: 2467 2813 2980 3217 3875 3900 4207 4297 4797 Size: 346 167 237 658 25 307 90 500 Cuts at: 4797 6369 2467 Size: 1572 3152 Fragments arranged by size: 3152 1572 658 500 346 307 237 167 90 25 ApaI G_GGCC'C Cuts at: 2350 3086 3158 3414 3859 2350 Size: 736 72 256 445 5545 Fragments arranged by size: 5545 736 445 256 72 ApaLI G'TGCA_C Cuts at: 4025 5111 6267 4025 Size: 1086 1156 4812 Fragments arranged by size: 4812 1156 1086 ApoI r'AATT_y Cuts at: 1493 4165 4363 4589 4741 6850 1493 Size: 2672 198 226 152 2109 1697 Fragments arranged by size: 2672 2109 1697 226 198 152 AseI AT'TA_AT Cuts at: 160 1032 2249 5608 160 Size: 872 1217 3359 1606 Fragments arranged by size: 3359 1606 1217 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7054 AvaI C'yCGr_G Cuts at: 1870 1943 2769 3192 4328 4379 1870 Size: 73 826 423 1136 51 4545 Fragments arranged by size: 4545 1136 826 423 73 51 BamHI G'GATC_C Cuts at: 2604 2692 3550 4384 4751 2604 Size: 88 858 834 367 4907 Fragments arranged by size: 4907 858 834 367 88 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2624 2667 2745 3372 3613 Size: 325 217 627 837 43 78 627 241 Cuts at: 3613 3666 4375 5830 618 Size: 53 709 1455 1842 Fragments arranged by size: 1842 1455 837 709 627 627 325 241 217 78 53 43 BanII G_rGCy'C Cuts at: 1391 2350 2577 3086 3158 3199 3229 3414 3859 Size: 959 227 509 72 41 30 185 445 Cuts at: 3859 3993 4373 1391 Size: 134 380 4072 Fragments arranged by size: 4072 959 509 445 380 227 185 134 72 41 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3022 928 Size: 971 1123 4960 Fragments arranged by size: 4960 1123 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3804 3838 5245 5279 1903 Size: 34 1867 34 1407 34 3678 Fragments arranged by size: 3678 1867 1407 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4946 6156 4946 Size: 1210 5844 BclI T'GATC_A Cuts at: 1548 2637 3787 1548 Size: 1089 1150 4815 Fragments arranged by size: 4815 1150 1089 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2470 5665 136 Size: 107 122 71 631 605 798 3195 1525 Fragments arranged by size: 3195 1525 798 631 605 122 107 71 BglII A'GATC_T Cuts at: 7049 7049 Size: 7054 BlpI GC'TnA_GC Cuts at: 2448 2806 4471 2448 Size: 358 1665 5031 Fragments arranged by size: 5031 1665 358 Bme1580I G_kGCm'C Cuts at: 1790 2350 2627 3086 3158 3306 3414 3618 3851 Size: 560 277 459 72 148 108 204 233 Cuts at: 3851 3859 4029 5115 6271 1790 Size: 8 170 1086 1156 2573 Fragments arranged by size: 2573 1156 1086 560 459 277 233 204 170 148 108 72 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7054 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2968 3293 3727 Size: 416 53 83 399 24 1551 325 434 Cuts at: 3727 3875 3900 4056 4785 5743 442 Size: 148 25 156 729 958 1753 Fragments arranged by size: 1753 1551 958 729 434 416 399 325 156 148 83 53 25 24 BmtI G_CTAG'C Cuts at: 1056 2376 1056 Size: 1320 5734 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2857 2857 Size: 7054 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2217 2321 2852 3183 3363 3385 3877 4098 Size: 978 104 531 331 180 22 492 221 Cuts at: 4098 5714 1239 Size: 1616 2579 Fragments arranged by size: 2579 1616 978 531 492 331 221 180 104 22 Bpu10I CC'TnA_GC Cuts at: 3406 3506 3864 3406 Size: 100 358 6596 Fragments arranged by size: 6596 358 100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5179 6044 6342 5179 Size: 865 298 5891 Fragments arranged by size: 5891 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5717 882 Size: 617 4218 2219 Fragments arranged by size: 4218 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4845 493 Size: 711 3641 2702 Fragments arranged by size: 3641 2702 711 BsaBI GATnn'nnATC Cuts at: 7048 7048 Size: 7054 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3888 4861 5243 5878 Size: 53 83 186 936 2355 973 382 635 Cuts at: 5878 275 Size: 1451 Fragments arranged by size: 2355 1451 973 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2664 3722 5487 5885 6159 6306 6717 2664 Size: 1058 1765 398 274 147 411 3001 Fragments arranged by size: 3001 1765 1058 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2516 2546 1178 Size: 30 165 30 1113 30 5686 Fragments arranged by size: 5686 1113 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2415 2849 4185 4334 4346 1186 Size: 1229 434 1336 149 12 3894 Fragments arranged by size: 3894 1336 1229 434 149 12 BseYI C'CCAG_C Cuts at: 2467 3228 3249 3555 3765 4030 4305 6257 6753 Size: 761 21 306 210 265 275 1952 496 Cuts at: 6753 2467 Size: 2768 Fragments arranged by size: 2768 1952 761 496 306 275 265 210 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3117 3168 3433 1163 Size: 573 1381 51 265 4784 Fragments arranged by size: 4784 1381 573 265 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4504 5265 6293 1540 Size: 275 186 2503 761 1028 2301 Fragments arranged by size: 2503 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4029 5115 5200 6271 4029 Size: 1086 85 1071 4812 Fragments arranged by size: 4812 1086 1071 85 BsmI GAATG_Cn' Cuts at: 4565 4658 4565 Size: 93 6961 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2350 2577 2627 3086 3158 3199 3229 Size: 399 560 227 50 459 72 41 30 Cuts at: 3229 3306 3414 3618 3851 3859 3993 4029 4373 Size: 77 108 204 233 8 134 36 344 Cuts at: 4373 5115 5200 6271 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 560 459 399 344 233 227 204 134 108 85 77 72 50 41 36 30 8 BspEI T'CCGG_A Cuts at: 6717 6717 Size: 7054 BspHI T'CATG_A Cuts at: 4943 6673 4943 Size: 1730 5324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4409 844 Size: 3565 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4941 1394 Size: 429 3118 3507 Fragments arranged by size: 3507 3118 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2284 5549 5723 65 Size: 1183 1036 3265 174 1396 Fragments arranged by size: 3265 1396 1183 1036 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3663 5698 5885 1700 Size: 110 1853 2035 187 2869 Fragments arranged by size: 2869 2035 1853 187 110 BsrGI T'GTAC_A Cuts at: 96 2532 96 Size: 2436 4618 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7054 BssSI C'ACGA_G Cuts at: 5114 6126 5114 Size: 1012 6042 BstAPI GCAn_nnn'nTGC Cuts at: 794 2667 4272 794 Size: 1873 1605 3576 Fragments arranged by size: 3576 1873 1605 BstBI TT'CG_AA Cuts at: 5858 5858 Size: 7054 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7054 BstXI CCAn_nnnn'nTGG Cuts at: 2830 3698 2830 Size: 868 6186 BstYI r'GATC_y Cuts at: 1972 2604 2692 3338 3550 3803 4384 4751 5136 Size: 632 88 646 212 253 581 367 385 Cuts at: 5136 5153 6605 6691 6703 7049 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 646 632 581 385 367 346 253 212 88 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3475 513 Size: 552 2410 4092 Fragments arranged by size: 4092 2410 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6315 Fragments arranged by size: 6315 683 56 BtsI GCAGTG_nn' Cuts at: 3105 3340 3373 3517 4285 4570 5364 5384 3105 Size: 235 33 144 768 285 794 20 4775 Fragments arranged by size: 4775 794 768 285 235 144 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7054 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7019 DraI TTT'AAA Cuts at: 4358 4358 Size: 7054 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3440 6061 809 Size: 507 2124 2621 1802 Fragments arranged by size: 2621 2124 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2014 2480 2585 Size: 54 1425 50 275 18 184 466 105 Cuts at: 2585 2733 2780 3043 4501 5390 7019 8 Size: 148 47 263 1458 889 1629 43 Fragments arranged by size: 1629 1458 1425 889 466 275 263 184 148 105 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4501 1537 Size: 275 2689 4090 Fragments arranged by size: 4090 2689 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3430 4984 1353 Size: 2077 1554 3423 Fragments arranged by size: 3423 2077 1554 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5641 6015 6161 387 Size: 1064 138 4052 374 146 1280 Fragments arranged by size: 4052 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 2321 2588 2684 2852 3183 3363 Size: 393 585 104 267 96 168 331 180 Cuts at: 3363 3385 3877 4098 5117 5714 6501 6769 1239 Size: 22 492 221 1019 597 787 268 1524 Fragments arranged by size: 1524 1019 787 597 585 492 393 331 268 267 221 180 168 104 96 22 EcoO109I rG'GnC_Cy Cuts at: 2347 2984 3083 3155 3321 3410 3411 3855 3856 Size: 637 99 72 166 89 1 444 1 Cuts at: 3856 3980 4151 4232 2347 Size: 124 171 81 5169 Fragments arranged by size: 5169 637 444 171 166 124 99 89 81 72 1 1 EcoRI G'AATT_C Cuts at: 4363 4741 4363 Size: 378 6676 FspI TGC'GCA Cuts at: 2660 5560 2660 Size: 2900 4154 HaeII r_GCGC'y Cuts at: 1678 3217 6201 1678 Size: 1539 2984 2531 Fragments arranged by size: 2984 2531 1539 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3009 3041 3389 3421 5695 Size: 32 135 32 1437 32 348 32 2274 Cuts at: 5695 5727 5769 5801 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2274 1437 348 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3445 3886 4398 4644 669 Size: 988 281 1507 441 512 246 3079 Fragments arranged by size: 3079 1507 988 512 441 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7054 HpaI GTT'AAC Cuts at: 4644 4644 Size: 7054 KpnI G_GTAC'C Cuts at: 2749 3376 4379 2749 Size: 627 1003 5424 Fragments arranged by size: 5424 1003 627 MfeI C'AATT_G Cuts at: 4653 4653 Size: 7054 MluI A'CGCG_T Cuts at: 6930 6930 Size: 7054 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2310 3831 6168 6352 1305 Size: 1005 1521 2337 184 2007 Fragments arranged by size: 2337 2007 1521 1005 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2016 2482 2587 2735 2782 Size: 54 1425 343 184 466 105 148 47 Cuts at: 2782 10 Size: 4282 Fragments arranged by size: 4282 1425 466 343 184 148 105 54 47 MslI CAynn'nnrTG Cuts at: 518 1166 2633 3237 5012 5371 5530 518 Size: 648 1467 604 1775 359 159 2042 Fragments arranged by size: 2042 1775 1467 648 604 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2029 2108 2266 2455 2467 2731 2757 Size: 417 6 79 158 189 12 264 26 Cuts at: 2757 2799 4470 5147 6295 6540 1606 Size: 42 1671 677 1148 245 2120 Fragments arranged by size: 2120 1671 1148 677 417 264 245 189 158 79 42 26 12 6 NcoI C'CATG_G Cuts at: 513 1065 3475 513 Size: 552 2410 4092 Fragments arranged by size: 4092 2410 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7054 NheI G'CTAG_C Cuts at: 1052 2372 1052 Size: 1320 5734 NotI GC'GGCC_GC Cuts at: 4501 4501 Size: 7054 NspI r_CATG'y Cuts at: 1132 1229 2944 3571 4412 5957 1132 Size: 97 1715 627 841 1545 2229 Fragments arranged by size: 2229 1715 1545 841 627 97 PasI CC'CwG_GG Cuts at: 1348 2980 3852 1348 Size: 1632 872 4550 Fragments arranged by size: 4550 1632 872 PciI A'CATG_T Cuts at: 5953 5953 Size: 7054 PflMI CCAn_nnn'nTGG Cuts at: 1108 2980 3119 3562 3875 4207 1108 Size: 1872 139 443 313 332 3955 Fragments arranged by size: 3955 1872 443 332 313 139 PfoI T'CCnGG_A Cuts at: 3089 3199 3089 Size: 110 6944 PmeI GTTT'AAAC Cuts at: 4358 4358 Size: 7054 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5123 5155 6636 6668 5123 Size: 32 1481 32 5509 Fragments arranged by size: 5509 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2984 3980 2984 Size: 996 6058 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7054 PsiI TTA'TAA Cuts at: 4624 4624 Size: 7054 PspOMI G'GGCC_C Cuts at: 2346 3082 3154 3410 3855 2346 Size: 736 72 256 445 5545 Fragments arranged by size: 5545 736 445 256 72 PspXI vC'TCGA_Gb Cuts at: 4328 4328 Size: 7054 PstI C_TGCA'G Cuts at: 830 2028 2070 2460 2762 2804 3500 3539 4406 Size: 1198 42 390 302 42 696 39 867 Cuts at: 4406 830 Size: 3478 Fragments arranged by size: 3478 1198 867 696 390 302 42 42 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7054 PvuII CAG'CTG Cuts at: 2023 2029 2455 2731 2757 2799 2023 Size: 6 426 276 26 42 6278 Fragments arranged by size: 6278 426 276 42 26 6 SalI G'TCGA_C Cuts at: 1936 4396 1936 Size: 2460 4594 SanDI GG'GwC_CC Cuts at: 2984 3980 2984 Size: 996 6058 SapI GCTCTTCn'nnn_ Cuts at: 3430 3430 Size: 7054 SbfI CC_TGCA'GG Cuts at: 2070 4406 2070 Size: 2336 4718 ScaI AGT'ACT Cuts at: 5302 7014 5302 Size: 1712 5342 SexAI A'CCwGG_T Cuts at: 3007 3118 3007 Size: 111 6943 SfcI C'TryA_G Cuts at: 826 1045 2024 2066 2456 2758 2800 3464 3496 Size: 219 979 42 390 302 42 664 32 Cuts at: 3496 3535 4402 5537 6218 6409 826 Size: 39 867 1135 681 191 1471 Fragments arranged by size: 1471 1135 979 867 681 664 390 302 219 191 42 42 39 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7054 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7054 SmaI CCC'GGG Cuts at: 1872 2771 3194 4381 1872 Size: 899 423 1187 4545 Fragments arranged by size: 4545 1187 899 423 SmlI C'TyrA_G Cuts at: 820 1017 1943 4328 5158 6059 6321 820 Size: 197 926 2385 830 901 262 1553 Fragments arranged by size: 2385 1553 926 901 830 262 197 SnaBI TAC'GTA Cuts at: 493 4845 493 Size: 4352 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7054 SphI G_CATG'C Cuts at: 1132 4412 1132 Size: 3280 3774 SspI AAT'ATT Cuts at: 5 52 4978 5 Size: 47 4926 2081 Fragments arranged by size: 4926 2081 47 StuI AGG'CCT Cuts at: 1517 3742 4119 1517 Size: 2225 377 4452 Fragments arranged by size: 4452 2225 377 StyI C'CwwG_G Cuts at: 513 1065 3475 3600 4076 4493 513 Size: 552 2410 125 476 417 3074 Fragments arranged by size: 3074 2410 552 476 417 125 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3972 4044 5081 5098 5251 5436 5881 Size: 1255 2392 72 1037 17 153 185 445 Cuts at: 5881 325 Size: 1498 Fragments arranged by size: 2392 1498 1255 1037 445 185 153 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 3531 5300 Size: 274 80 33 51 1443 555 999 1769 Cuts at: 5300 7012 96 Size: 1712 138 Fragments arranged by size: 1769 1712 1443 999 555 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2377 2409 3254 3286 7014 7046 2377 Size: 32 845 32 3728 32 2385 Fragments arranged by size: 3728 2385 845 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3120 1316 Size: 1804 5250 XbaI T'CTAG_A Cuts at: 4390 4390 Size: 7054 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2728 3436 2728 Size: 708 6346 XhoI C'TCGA_G Cuts at: 1943 4328 1943 Size: 2385 4669 XmaI C'CCGG_G Cuts at: 1870 2769 3192 4379 1870 Size: 899 423 1187 4545 Fragments arranged by size: 4545 1187 899 423 XmnI GAAnn'nnTTC Cuts at: 5183 5183 Size: 7054 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4862 276 Size: 53 83 186 936 3328 2468 Fragments arranged by size: 3328 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI