(Circular) (Six-base) MAPSORT of: pFN21AB6253.seq Check: 8387 from: 1 to: 6625 pFN21AB6253 6625 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2932 2932 Size: 6625 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4435 278 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3968 1937 Size: 2031 4594 Acc65I G'GTAC_C Cuts at: 3946 3946 Size: 6625 AclI AA'CG_TT Cuts at: 1563 2344 4752 5125 1563 Size: 781 2408 373 3063 Fragments arranged by size: 3063 2408 781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2177 2775 3120 3476 3593 4688 6072 6340 Size: 545 598 345 356 117 1095 1384 268 Cuts at: 6340 1632 Size: 1917 Fragments arranged by size: 1917 1384 1095 598 545 356 345 268 117 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6428 AflIII A'CryG_T Cuts at: 1203 2800 5524 6501 1203 Size: 1597 2724 977 1327 Fragments arranged by size: 2724 1597 1327 977 AgeI A'CCGG_T Cuts at: 5456 5456 Size: 6625 AhdI GACnn_n'nnGTC Cuts at: 5354 5354 Size: 6625 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6625 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6625 AlwNI CAG_nnn'CTG Cuts at: 3825 4368 5940 3825 Size: 543 1572 4510 Fragments arranged by size: 4510 1572 543 ApaLI G'TGCA_C Cuts at: 3030 4682 5838 3030 Size: 1652 1156 3817 Fragments arranged by size: 3817 1652 1156 ApoI r'AATT_y Cuts at: 1493 2560 3176 3934 4160 4312 6421 1493 Size: 1067 616 758 226 152 2109 1697 Fragments arranged by size: 2109 1697 1067 758 616 226 152 AseI AT'TA_AT Cuts at: 160 1032 5179 160 Size: 872 4147 1606 Fragments arranged by size: 4147 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 AvaI C'yCGr_G Cuts at: 1870 1943 2136 2978 3950 1870 Size: 73 193 842 972 4545 Fragments arranged by size: 4545 972 842 193 73 BamHI G'GATC_C Cuts at: 3082 3955 4322 3082 Size: 873 367 5385 Fragments arranged by size: 5385 873 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3143 3730 3946 5401 618 Size: 325 217 627 1356 587 216 1455 1842 Fragments arranged by size: 1842 1455 1356 627 587 325 217 216 BanII G_rGCy'C Cuts at: 1391 2274 3803 3944 1391 Size: 883 1529 141 4072 Fragments arranged by size: 4072 1529 883 141 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2020 2463 2601 2871 3756 928 Size: 971 121 443 138 270 885 3797 Fragments arranged by size: 3797 971 885 443 270 138 121 BbvCI CC'TCA_GC Cuts at: 2823 2823 Size: 6625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 4816 4850 1903 Size: 34 1850 34 995 34 3678 Fragments arranged by size: 3678 1850 995 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4517 5727 4517 Size: 1210 5415 BclI T'GATC_A Cuts at: 1548 3507 1548 Size: 1959 4666 BfrBI ATG'CAT Cuts at: 3899 3899 Size: 6625 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5236 136 Size: 107 122 71 631 605 3564 1525 Fragments arranged by size: 3564 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3493 6620 3493 Size: 3127 3498 BlpI GC'TnA_GC Cuts at: 4042 4042 Size: 6625 Bme1580I G_kGCm'C Cuts at: 1790 3034 3146 4686 5842 1790 Size: 1244 112 1540 1156 2573 Fragments arranged by size: 2573 1540 1244 1156 112 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6625 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2498 3649 4356 Size: 416 53 83 399 24 1081 1151 707 Cuts at: 4356 5314 442 Size: 958 1753 Fragments arranged by size: 1753 1151 1081 958 707 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6625 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2755 5285 1239 Size: 1516 2530 2579 Fragments arranged by size: 2579 2530 1516 Bpu10I CC'TnA_GC Cuts at: 2268 2676 2823 2268 Size: 408 147 6070 Fragments arranged by size: 6070 408 147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3417 4750 5615 5913 3417 Size: 1333 865 298 4129 Fragments arranged by size: 4129 1333 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3309 5288 882 Size: 617 1810 1979 2219 Fragments arranged by size: 2219 1979 1810 617 BsaAI yAC'GTr Cuts at: 493 1204 3892 4416 493 Size: 711 2688 524 2702 Fragments arranged by size: 2702 2688 711 524 BsaBI GATnn'nnATC Cuts at: 6619 6619 Size: 6625 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4432 4814 5449 275 Size: 53 83 186 936 2899 382 635 1451 Fragments arranged by size: 2899 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5058 5456 5730 5877 6288 5058 Size: 398 274 147 411 5395 Fragments arranged by size: 5395 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6400 Fragments arranged by size: 6400 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3869 1186 Size: 2683 3942 BseYI C'CCAG_C Cuts at: 3361 5828 6324 3361 Size: 2467 496 3662 Fragments arranged by size: 3662 2467 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2252 1163 Size: 573 516 5536 Fragments arranged by size: 5536 573 516 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4075 4836 5864 1540 Size: 275 186 2074 761 1028 2301 Fragments arranged by size: 2301 2074 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2274 3034 4686 4771 5842 2274 Size: 760 1652 85 1071 3057 Fragments arranged by size: 3057 1652 1071 760 85 BsmI GAATG_Cn' Cuts at: 3899 3901 4136 4229 3899 Size: 2 235 93 6295 Fragments arranged by size: 6295 235 93 2 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2274 3034 3146 3803 3944 4686 4771 Size: 399 484 760 112 657 141 742 85 Cuts at: 4771 5842 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 760 742 657 484 399 141 112 85 BspEI T'CCGG_A Cuts at: 6288 6288 Size: 6625 BspHI T'CATG_A Cuts at: 4514 6244 4514 Size: 1730 4895 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2932 3841 3980 844 Size: 2088 909 139 3489 Fragments arranged by size: 3489 2088 909 139 BsrBI CCG'CTC Cuts at: 1394 1823 4512 1394 Size: 429 2689 3507 Fragments arranged by size: 3507 2689 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2997 5120 5294 65 Size: 1183 1749 2123 174 1396 Fragments arranged by size: 2123 1749 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5269 5456 1700 Size: 110 3459 187 2869 Fragments arranged by size: 3459 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2323 96 Size: 2227 4398 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6625 BssSI C'ACGA_G Cuts at: 4685 5697 4685 Size: 1012 5613 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6625 BstBI TT'CG_AA Cuts at: 5429 5429 Size: 6625 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6625 BstYI r'GATC_y Cuts at: 1972 3082 3493 3955 4322 4707 4724 6176 6262 Size: 1110 411 462 367 385 17 1452 86 Cuts at: 6262 6274 6620 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1110 462 411 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5134 Fragments arranged by size: 5134 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5886 Fragments arranged by size: 5886 683 56 BtsI GCAGTG_nn' Cuts at: 2044 2240 2637 4141 4935 4955 2044 Size: 196 397 1504 794 20 3714 Fragments arranged by size: 3714 1504 794 397 196 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6625 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6590 DraI TTT'AAA Cuts at: 3929 3929 Size: 6625 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5632 809 Size: 507 4316 1802 Fragments arranged by size: 4316 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2634 2710 4072 Size: 54 1425 50 275 18 804 76 1362 Cuts at: 4072 4961 6590 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1362 889 804 275 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4072 1537 Size: 275 2260 4090 Fragments arranged by size: 4090 2260 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2136 2445 4555 1353 Size: 783 309 2110 3423 Fragments arranged by size: 3423 2110 783 309 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2673 5212 5586 5732 387 Size: 1064 138 1084 2539 374 146 1280 Fragments arranged by size: 2539 1280 1084 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2272 2272 Size: 6625 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2755 2775 3120 3476 3593 4688 Size: 393 545 578 20 345 356 117 1095 Cuts at: 4688 5285 6072 6340 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1095 787 597 578 545 393 356 345 268 117 20 EcoO109I rG'GnC_Cy Cuts at: 2282 3789 2282 Size: 1507 5118 EcoRI G'AATT_C Cuts at: 2560 3934 4312 2560 Size: 1374 378 4873 Fragments arranged by size: 4873 1374 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2131 3187 2131 Size: 1056 5569 FspI TGC'GCA Cuts at: 5131 5131 Size: 6625 HaeII r_GCGC'y Cuts at: 1678 3111 5772 1678 Size: 1433 2661 2531 Fragments arranged by size: 2661 2531 1433 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3074 3106 3451 3463 3483 Size: 32 135 32 1502 32 345 12 20 Cuts at: 3483 3495 3581 3613 5266 5298 5340 5372 1373 Size: 12 86 32 1653 32 42 32 2626 Fragments arranged by size: 2626 1653 1502 345 135 86 42 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2725 2916 3969 4215 669 Size: 988 281 787 191 1053 246 3079 Fragments arranged by size: 3079 1053 988 787 281 246 191 HindIII A'AGCT_T Cuts at: 748 2641 748 Size: 1893 4732 HpaI GTT'AAC Cuts at: 2725 4215 2725 Size: 1490 5135 KpnI G_GTAC'C Cuts at: 3950 3950 Size: 6625 MfeI C'AATT_G Cuts at: 4224 4224 Size: 6625 MluI A'CGCG_T Cuts at: 6501 6501 Size: 6625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2555 3626 5739 5923 1305 Size: 1250 1071 2113 184 2007 Fragments arranged by size: 2113 2007 1250 1071 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2636 2712 10 Size: 54 1425 343 804 76 3923 Fragments arranged by size: 3923 1425 804 343 76 54 MslI CAynn'nnrTG Cuts at: 518 1166 2079 2856 4583 4942 5101 518 Size: 648 913 777 1727 359 159 2042 Fragments arranged by size: 2042 1727 913 777 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2831 3080 4041 4718 5866 6111 1606 Size: 1225 249 961 677 1148 245 2120 Fragments arranged by size: 2120 1225 1148 961 677 249 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5134 Fragments arranged by size: 5134 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6625 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6625 NotI GC'GGCC_GC Cuts at: 4072 4072 Size: 6625 NsiI A_TGCA'T Cuts at: 3901 3901 Size: 6625 NspI r_CATG'y Cuts at: 1132 1229 2804 2970 3422 3983 5528 1132 Size: 97 1575 166 452 561 1545 2229 Fragments arranged by size: 2229 1575 1545 561 452 166 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6625 PciI A'CATG_T Cuts at: 2800 5524 2800 Size: 2724 3901 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6625 PfoI T'CCnGG_A Cuts at: 2441 2441 Size: 6625 PmeI GTTT'AAAC Cuts at: 3929 3929 Size: 6625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4694 4726 6207 6239 4694 Size: 32 1481 32 5080 Fragments arranged by size: 5080 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2282 3789 2282 Size: 1507 5118 PshAI GACnn'nnGTC Cuts at: 1613 3917 1613 Size: 2304 4321 PsiI TTA'TAA Cuts at: 4195 4195 Size: 6625 PstI C_TGCA'G Cuts at: 830 2604 3501 3977 830 Size: 1774 897 476 3478 Fragments arranged by size: 3478 1774 897 476 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6625 PvuII CAG'CTG Cuts at: 2831 2831 Size: 6625 RsrII CG'GwC_CG Cuts at: 2336 2336 Size: 6625 SacI G_AGCT'C Cuts at: 2274 2274 Size: 6625 SalI G'TCGA_C Cuts at: 1936 3967 1936 Size: 2031 4594 SbfI CC_TGCA'GG Cuts at: 3977 3977 Size: 6625 ScaI AGT'ACT Cuts at: 2259 4873 6585 2259 Size: 2614 1712 2299 Fragments arranged by size: 2614 2299 1712 SfcI C'TryA_G Cuts at: 826 1045 2600 2688 2790 3497 3973 5108 5789 Size: 219 1555 88 102 707 476 1135 681 Cuts at: 5789 5980 826 Size: 191 1471 Fragments arranged by size: 1555 1471 1135 707 681 476 219 191 102 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6625 SmaI CCC'GGG Cuts at: 1872 2138 2980 3952 1872 Size: 266 842 972 4545 Fragments arranged by size: 4545 972 842 266 SmlI C'TyrA_G Cuts at: 820 1017 1943 3432 4729 5630 5892 820 Size: 197 926 1489 1297 901 262 1553 Fragments arranged by size: 1553 1489 1297 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4416 493 Size: 3923 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6625 SphI G_CATG'C Cuts at: 1132 3983 1132 Size: 2851 3774 SspI AAT'ATT Cuts at: 5 52 3706 4549 5 Size: 47 3654 843 2081 Fragments arranged by size: 3654 2081 843 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6625 StyI C'CwwG_G Cuts at: 513 1065 2004 2285 4064 513 Size: 552 939 281 1779 3074 Fragments arranged by size: 3074 1779 939 552 281 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4652 4669 4822 5007 5452 325 Size: 1255 3072 17 153 185 445 1498 Fragments arranged by size: 3072 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2257 2323 4871 Size: 274 80 33 51 1443 280 66 2548 Cuts at: 4871 6583 96 Size: 1712 138 Fragments arranged by size: 2548 1712 1443 280 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2928 2960 6585 6617 2928 Size: 32 3625 32 2936 Fragments arranged by size: 3625 2936 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6625 XbaI T'CTAG_A Cuts at: 3961 3961 Size: 6625 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2081 2081 Size: 6625 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6625 XmaI C'CCGG_G Cuts at: 1870 2136 2978 3950 1870 Size: 266 842 972 4545 Fragments arranged by size: 4545 972 842 266 XmnI GAAnn'nnTTC Cuts at: 3899 4754 3899 Size: 855 5770 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4433 276 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI