(Circular) (Six-base) MAPSORT of: pFN21AB6243.seq Check: 4468 from: 1 to: 6964 pFN21AB6243 6964 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4774 278 Size: 53 83 186 936 3238 2468 Fragments arranged by size: 3238 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4307 1937 Size: 2370 4594 Acc65I G'GTAC_C Cuts at: 2421 4285 2421 Size: 1864 5100 AclI AA'CG_TT Cuts at: 1563 2687 5091 5464 1563 Size: 1124 2404 373 3063 Fragments arranged by size: 3063 2404 1124 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2724 4026 4101 5027 6411 6679 1632 Size: 1092 1302 75 926 1384 268 1917 Fragments arranged by size: 1917 1384 1302 1092 926 268 75 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6767 AflIII A'CryG_T Cuts at: 1203 5863 6840 1203 Size: 4660 977 1327 Fragments arranged by size: 4660 1327 977 AgeI A'CCGG_T Cuts at: 5795 5795 Size: 6964 AhdI GACnn_n'nnGTC Cuts at: 4189 5693 4189 Size: 1504 5460 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6964 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6964 AlwNI CAG_nnn'CTG Cuts at: 3047 3610 4049 4180 4707 6279 3047 Size: 563 439 131 527 1572 3732 Fragments arranged by size: 3732 1572 563 527 439 131 ApaI G_GGCC'C Cuts at: 4011 4011 Size: 6964 ApaLI G'TGCA_C Cuts at: 2582 5021 6177 2582 Size: 2439 1156 3369 Fragments arranged by size: 3369 2439 1156 ApoI r'AATT_y Cuts at: 1493 3061 3133 3241 3768 4273 4499 4651 6760 Size: 1568 72 108 527 505 226 152 2109 Cuts at: 6760 1493 Size: 1697 Fragments arranged by size: 2109 1697 1568 527 505 226 152 108 72 AseI AT'TA_AT Cuts at: 160 1032 5518 160 Size: 872 4486 1606 Fragments arranged by size: 4486 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6964 AvaI C'yCGr_G Cuts at: 1870 1943 4055 4289 1870 Size: 73 2112 234 4545 Fragments arranged by size: 4545 2112 234 73 AvrII C'CTAG_G Cuts at: 3349 3349 Size: 6964 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2413 2446 3798 3831 2413 Size: 33 1352 33 5546 Fragments arranged by size: 5546 1352 33 33 BamHI G'GATC_C Cuts at: 2568 4294 4661 2568 Size: 1726 367 4871 Fragments arranged by size: 4871 1726 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2110 2421 3475 3620 3737 Size: 325 217 627 323 311 1054 145 117 Cuts at: 3737 4285 5740 618 Size: 548 1455 1842 Fragments arranged by size: 1842 1455 1054 627 548 325 323 311 217 145 117 BanII G_rGCy'C Cuts at: 1391 3588 3842 3992 4011 4283 1391 Size: 2197 254 150 19 272 4072 Fragments arranged by size: 4072 2197 272 254 150 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2495 928 Size: 971 596 5397 Fragments arranged by size: 5397 971 596 BbvCI CC'TCA_GC Cuts at: 3089 3316 3089 Size: 227 6737 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5155 5189 1903 Size: 34 3218 34 3678 Fragments arranged by size: 3678 3218 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2989 4856 6066 2989 Size: 1867 1210 3887 Fragments arranged by size: 3887 1867 1210 BclI T'GATC_A Cuts at: 1548 3873 1548 Size: 2325 4639 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5575 136 Size: 107 122 71 631 605 3903 1525 Fragments arranged by size: 3903 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6959 6959 Size: 6964 BlpI GC'TnA_GC Cuts at: 4381 4381 Size: 6964 Bme1580I G_kGCm'C Cuts at: 1790 2586 4011 5025 6181 1790 Size: 796 1425 1014 1156 2573 Fragments arranged by size: 2573 1425 1156 1014 796 BmgBI CAC'GTC Cuts at: 1318 2614 1318 Size: 1296 5668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2874 4695 5653 Size: 416 53 83 399 24 1457 1821 958 Cuts at: 5653 442 Size: 1753 Fragments arranged by size: 1821 1753 1457 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6964 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3390 3963 4157 5624 1239 Size: 2151 573 194 1467 2579 Fragments arranged by size: 2579 2151 1467 573 194 Bpu10I CC'TnA_GC Cuts at: 2084 3089 3316 2084 Size: 1005 227 5732 Fragments arranged by size: 5732 1005 227 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3046 5089 5954 6252 3046 Size: 2043 865 298 3758 Fragments arranged by size: 3758 2043 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5627 882 Size: 617 4128 2219 Fragments arranged by size: 4128 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2575 4755 493 Size: 711 1371 2180 2702 Fragments arranged by size: 2702 2180 1371 711 BsaBI GATnn'nnATC Cuts at: 3000 3878 6958 3000 Size: 878 3080 3006 Fragments arranged by size: 3080 3006 878 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4771 5153 5788 275 Size: 53 83 186 936 3238 382 635 1451 Fragments arranged by size: 3238 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2392 5397 5795 6069 6216 6627 2392 Size: 3005 398 274 147 411 2729 Fragments arranged by size: 3005 2729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3652 3682 3733 3763 1178 Size: 30 165 30 2249 30 51 30 4379 Fragments arranged by size: 4379 2249 165 51 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2514 3321 3955 4072 4244 4247 1186 Size: 1328 807 634 117 172 3 3903 Fragments arranged by size: 3903 1328 807 634 172 117 3 BseYI C'CCAG_C Cuts at: 3297 3363 3512 4010 4024 6167 6663 3297 Size: 66 149 498 14 2143 496 3598 Fragments arranged by size: 3598 2143 498 496 149 66 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2306 2636 1163 Size: 573 570 330 5491 Fragments arranged by size: 5491 573 570 330 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4414 5175 6203 1540 Size: 275 186 2413 761 1028 2301 Fragments arranged by size: 2413 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2586 3842 5025 5110 6181 2586 Size: 1256 1183 85 1071 3369 Fragments arranged by size: 3369 1256 1183 1071 85 BsmI GAATG_Cn' Cuts at: 2633 4475 4568 2633 Size: 1842 93 5029 Fragments arranged by size: 5029 1842 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2586 3588 3842 3992 4011 4283 5025 Size: 399 796 1002 254 150 19 272 742 Cuts at: 5025 5110 6181 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1002 796 742 399 272 254 150 85 19 BspEI T'CCGG_A Cuts at: 2392 6627 2392 Size: 4235 2729 BspHI T'CATG_A Cuts at: 2260 2941 4853 6583 2260 Size: 681 1912 1730 2641 Fragments arranged by size: 2641 1912 1730 681 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2481 3319 4319 844 Size: 1637 838 1000 3489 Fragments arranged by size: 3489 1637 1000 838 BsrBI CCG'CTC Cuts at: 1394 1823 3937 4851 1394 Size: 429 2114 914 3507 Fragments arranged by size: 3507 2114 914 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2274 5459 5633 65 Size: 1183 1026 3185 174 1396 Fragments arranged by size: 3185 1396 1183 1026 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5608 5795 1700 Size: 110 3798 187 2869 Fragments arranged by size: 3798 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2646 96 Size: 2550 4414 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6964 BssSI C'ACGA_G Cuts at: 5024 6036 5024 Size: 1012 5952 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6964 BstBI TT'CG_AA Cuts at: 2755 5768 2755 Size: 3013 3951 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6964 BstXI CCAn_nnnn'nTGG Cuts at: 3854 3854 Size: 6964 BstYI r'GATC_y Cuts at: 1972 2568 3160 4294 4661 5046 5063 6515 6601 Size: 596 592 1134 367 385 17 1452 86 Cuts at: 6601 6613 6959 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1134 596 592 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2564 2564 Size: 6964 BtgI C'CryG_G Cuts at: 513 1065 2004 3597 4146 513 Size: 552 939 1593 549 3331 Fragments arranged by size: 3331 1593 939 552 549 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6225 Fragments arranged by size: 6225 683 56 BtsI GCAGTG_nn' Cuts at: 3543 3807 4480 5274 5294 3543 Size: 264 673 794 20 5213 Fragments arranged by size: 5213 794 673 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6964 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6929 DraI TTT'AAA Cuts at: 4268 4268 Size: 6964 DraIII CAC_nnn'GTG Cuts at: 2735 2735 Size: 6964 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5971 809 Size: 507 4655 1802 Fragments arranged by size: 4655 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4411 5300 6929 Size: 54 1425 50 275 18 2581 889 1629 Cuts at: 6929 8 Size: 43 Fragments arranged by size: 2581 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4411 1537 Size: 275 2599 4090 Fragments arranged by size: 4090 2599 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2232 3264 3885 4045 4894 1353 Size: 879 1032 621 160 849 3423 Fragments arranged by size: 3423 1032 879 849 621 160 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3216 3324 5551 5925 6071 387 Size: 1064 138 1627 108 2227 374 146 1280 Fragments arranged by size: 2227 1627 1280 1064 374 146 138 108 EcoICRI GAG'CTC Cuts at: 3840 3840 Size: 6964 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2724 3390 3963 4026 4101 4157 5027 Size: 393 1092 666 573 63 75 56 870 Cuts at: 5027 5624 6411 6679 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1092 870 787 666 597 573 393 268 75 63 56 EcoO109I rG'GnC_Cy Cuts at: 2164 3085 3353 2164 Size: 921 268 5775 Fragments arranged by size: 5775 921 268 EcoRI G'AATT_C Cuts at: 3133 4273 4651 3133 Size: 1140 378 5446 Fragments arranged by size: 5446 1140 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2564 3476 2564 Size: 912 6052 FspI TGC'GCA Cuts at: 5470 5470 Size: 6964 HaeII r_GCGC'y Cuts at: 1678 6111 1678 Size: 4433 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3194 3226 3926 3958 4151 Size: 32 135 32 1622 32 700 32 193 Cuts at: 4151 4175 4183 4207 5605 5637 5679 5711 1373 Size: 24 8 24 1398 32 42 32 2626 Fragments arranged by size: 2626 1622 1398 700 193 135 42 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 4308 4554 669 Size: 988 281 2370 246 3079 Fragments arranged by size: 3079 2370 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6964 HpaI GTT'AAC Cuts at: 4554 4554 Size: 6964 KpnI G_GTAC'C Cuts at: 2425 4289 2425 Size: 1864 5100 MfeI C'AATT_G Cuts at: 4563 4563 Size: 6964 MluI A'CGCG_T Cuts at: 6840 6840 Size: 6964 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2364 6078 6262 1305 Size: 1059 3714 184 2007 Fragments arranged by size: 3714 2007 1059 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5142 Fragments arranged by size: 5142 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2342 2605 2782 2856 3901 4151 4922 Size: 648 1176 263 177 74 1045 250 771 Cuts at: 4922 5281 5440 518 Size: 359 159 2042 Fragments arranged by size: 2042 1176 1045 771 648 359 263 250 177 159 74 MspA1I CmG'CkG Cuts at: 1606 3533 3548 4380 5057 6205 6450 1606 Size: 1927 15 832 677 1148 245 2120 Fragments arranged by size: 2120 1927 1148 832 677 245 15 NcoI C'CATG_G Cuts at: 513 1065 2004 3597 4146 513 Size: 552 939 1593 549 3331 Fragments arranged by size: 3331 1593 939 552 549 NdeI CA'TA_TG Cuts at: 387 387 Size: 6964 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6964 NotI GC'GGCC_GC Cuts at: 4411 4411 Size: 6964 NspI r_CATG'y Cuts at: 1132 1229 3900 3918 4322 5867 1132 Size: 97 2671 18 404 1545 2229 Fragments arranged by size: 2671 2229 1545 404 97 18 PasI CC'CwG_GG Cuts at: 1348 2275 3076 3616 3833 1348 Size: 927 801 540 217 4479 Fragments arranged by size: 4479 927 801 540 217 PciI A'CATG_T Cuts at: 5863 5863 Size: 6964 PflMI CCAn_nnn'nTGG Cuts at: 1108 4180 1108 Size: 3072 3892 PmeI GTTT'AAAC Cuts at: 4268 4268 Size: 6964 PmlI CAC'GTG Cuts at: 2575 2575 Size: 6964 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5033 5065 6546 6578 5033 Size: 32 1481 32 5419 Fragments arranged by size: 5419 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2164 3085 3353 2164 Size: 921 268 5775 Fragments arranged by size: 5775 921 268 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6964 PsiI TTA'TAA Cuts at: 4534 4534 Size: 6964 PspOMI G'GGCC_C Cuts at: 4007 4007 Size: 6964 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3702 3734 3702 Size: 32 6932 PstI C_TGCA'G Cuts at: 830 2382 2714 3690 3814 4020 4224 4316 830 Size: 1552 332 976 124 206 204 92 3478 Fragments arranged by size: 3478 1552 976 332 206 204 124 92 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6964 PvuII CAG'CTG Cuts at: 3548 3548 Size: 6964 SacI G_AGCT'C Cuts at: 3842 3842 Size: 6964 SalI G'TCGA_C Cuts at: 1936 4306 1936 Size: 2370 4594 SbfI CC_TGCA'GG Cuts at: 4316 4316 Size: 6964 ScaI AGT'ACT Cuts at: 3577 5212 6924 3577 Size: 1635 1712 3617 Fragments arranged by size: 3617 1712 1635 SfcI C'TryA_G Cuts at: 826 1045 2378 2455 2710 3686 3810 4016 4220 Size: 219 1333 77 255 976 124 206 204 Cuts at: 4220 4312 5447 6128 6319 826 Size: 92 1135 681 191 1471 Fragments arranged by size: 1471 1333 1135 976 681 255 219 206 204 191 124 92 77 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6964 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6964 SmaI CCC'GGG Cuts at: 1872 4057 4291 1872 Size: 2185 234 4545 Fragments arranged by size: 4545 2185 234 SmlI C'TyrA_G Cuts at: 820 1017 1943 3025 5068 5969 6231 820 Size: 197 926 1082 2043 901 262 1553 Fragments arranged by size: 2043 1553 1082 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4755 493 Size: 4262 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6964 SphI G_CATG'C Cuts at: 1132 3918 4322 1132 Size: 2786 404 3774 Fragments arranged by size: 3774 2786 404 SspI AAT'ATT Cuts at: 5 52 2356 4888 5 Size: 47 2304 2532 2081 Fragments arranged by size: 2532 2304 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6964 StyI C'CwwG_G Cuts at: 513 1065 2004 3349 3597 4146 4260 4403 513 Size: 552 939 1345 248 549 114 143 3074 Fragments arranged by size: 3074 1345 939 552 549 248 143 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2356 3608 4991 5008 5161 5346 5791 Size: 1255 776 1252 1383 17 153 185 445 Cuts at: 5791 325 Size: 1498 Fragments arranged by size: 1498 1383 1255 1252 776 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2505 2646 3268 Size: 274 80 33 51 1443 528 141 622 Cuts at: 3268 3575 5210 6922 96 Size: 307 1635 1712 138 Fragments arranged by size: 1712 1635 1443 622 528 307 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3652 3684 3733 3765 6924 6956 3652 Size: 32 49 32 3159 32 3660 Fragments arranged by size: 3660 3159 49 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6964 XbaI T'CTAG_A Cuts at: 4300 4300 Size: 6964 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3700 4001 3700 Size: 301 6663 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6964 XmaI C'CCGG_G Cuts at: 1870 4055 4289 1870 Size: 2185 234 4545 Fragments arranged by size: 4545 2185 234 XmnI GAAnn'nnTTC Cuts at: 2633 2900 3768 5093 2633 Size: 267 868 1325 4504 Fragments arranged by size: 4504 1325 868 267 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4772 276 Size: 53 83 186 936 3238 2468 Fragments arranged by size: 3238 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI