(Circular) (Six-base) MAPSORT of: pFN21AB6237.seq Check: 9442 from: 1 to: 6958 pFN21AB6237 6958 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4768 278 Size: 53 83 186 936 3232 2468 Fragments arranged by size: 3232 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4301 1937 Size: 2364 4594 Acc65I G'GTAC_C Cuts at: 2291 4279 2291 Size: 1988 4970 AclI AA'CG_TT Cuts at: 1563 5085 5458 1563 Size: 3522 373 3063 Fragments arranged by size: 3522 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2376 2764 3268 3436 3688 4192 5021 6405 Size: 744 388 504 168 252 504 829 1384 Cuts at: 6405 6673 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 829 744 504 504 388 268 252 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6761 AflIII A'CryG_T Cuts at: 1203 5857 6834 1203 Size: 4654 977 1327 Fragments arranged by size: 4654 1327 977 AgeI A'CCGG_T Cuts at: 5789 5789 Size: 6958 AhdI GACnn_n'nnGTC Cuts at: 5687 5687 Size: 6958 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6958 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6958 AlwNI CAG_nnn'CTG Cuts at: 2345 2482 3459 4243 4701 6273 2345 Size: 137 977 784 458 1572 3030 Fragments arranged by size: 3030 1572 977 784 458 137 ApaI G_GGCC'C Cuts at: 3210 3210 Size: 6958 ApaLI G'TGCA_C Cuts at: 5015 6171 5015 Size: 1156 5802 ApoI r'AATT_y Cuts at: 1493 2549 2638 2814 2898 2982 3066 3150 3402 Size: 1056 89 176 84 84 84 84 252 Cuts at: 3402 3486 3654 3822 3990 4158 4267 4493 4645 Size: 84 168 168 168 168 109 226 152 Cuts at: 4645 6754 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1056 252 226 176 168 168 168 168 152 109 89 84 84 84 84 84 AseI AT'TA_AT Cuts at: 160 1032 5512 160 Size: 872 4480 1606 Fragments arranged by size: 4480 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6958 AvaI C'yCGr_G Cuts at: 1870 1943 4283 1870 Size: 73 2340 4545 Fragments arranged by size: 4545 2340 73 BamHI G'GATC_C Cuts at: 4288 4655 4288 Size: 367 6591 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2291 4279 5734 618 Size: 325 217 627 504 1988 1455 1842 Fragments arranged by size: 1988 1842 1455 627 504 325 217 BanII G_rGCy'C Cuts at: 1391 3210 3968 4277 1391 Size: 1819 758 309 4072 Fragments arranged by size: 4072 1819 758 309 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5987 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5149 5183 1903 Size: 34 3212 34 3678 Fragments arranged by size: 3678 3212 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4850 6060 4850 Size: 1210 5748 BclI T'GATC_A Cuts at: 1548 2845 1548 Size: 1297 5661 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3042 5569 136 Size: 107 122 71 631 605 1370 2527 1525 Fragments arranged by size: 2527 1525 1370 631 605 122 107 71 BglII A'GATC_T Cuts at: 2011 6953 2011 Size: 4942 2016 BlpI GC'TnA_GC Cuts at: 3642 4375 3642 Size: 733 6225 Bme1580I G_kGCm'C Cuts at: 1790 3210 5019 6175 1790 Size: 1420 1809 1156 2573 Fragments arranged by size: 2573 1809 1420 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6958 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2233 2314 2514 Size: 416 53 83 399 24 816 81 200 Cuts at: 2514 2642 3336 4008 4689 5647 442 Size: 128 694 672 681 958 1753 Fragments arranged by size: 1753 958 816 694 681 672 416 399 200 128 83 81 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6958 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3651 3651 Size: 6958 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2007 2355 2586 2760 2844 2928 3180 3432 Size: 768 348 231 174 84 84 252 252 Cuts at: 3432 3684 3768 4104 5618 1239 Size: 252 84 336 1514 2579 Fragments arranged by size: 2579 1514 768 348 336 252 252 252 231 174 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3564 5083 5948 6246 3564 Size: 1519 865 298 4276 Fragments arranged by size: 4276 1519 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2203 2603 5621 882 Size: 617 704 400 3018 2219 Fragments arranged by size: 3018 2219 704 617 400 BsaAI yAC'GTr Cuts at: 493 1204 4749 493 Size: 711 3545 2702 Fragments arranged by size: 3545 2702 711 BsaBI GATnn'nnATC Cuts at: 6952 6952 Size: 6958 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4765 5147 5782 275 Size: 53 83 186 936 3232 382 635 1451 Fragments arranged by size: 3232 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5391 5789 6063 6210 6621 5391 Size: 398 274 147 411 5728 Fragments arranged by size: 5728 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6733 Fragments arranged by size: 6733 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6958 BseYI C'CCAG_C Cuts at: 6161 6657 6161 Size: 496 6462 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2319 3369 1163 Size: 573 583 1050 4752 Fragments arranged by size: 4752 1050 583 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4408 5169 6197 1540 Size: 275 186 2407 761 1028 2301 Fragments arranged by size: 2407 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3128 3968 4052 4136 5019 5104 6175 3128 Size: 840 84 84 883 85 1071 3911 Fragments arranged by size: 3911 1071 883 840 85 84 84 BsiWI C'GTAC_G Cuts at: 3255 3255 Size: 6958 BsmI GAATG_Cn' Cuts at: 2280 3266 3518 3785 4115 4469 4562 2280 Size: 986 252 267 330 354 93 4676 Fragments arranged by size: 4676 986 354 330 267 252 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3128 3210 3968 4052 4136 4277 5019 Size: 399 1338 82 758 84 84 141 742 Cuts at: 5019 5104 6175 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1338 1071 758 742 399 141 85 84 84 82 BspEI T'CCGG_A Cuts at: 6621 6621 Size: 6958 BspHI T'CATG_A Cuts at: 3685 4847 6577 3685 Size: 1162 1730 4066 Fragments arranged by size: 4066 1730 1162 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4313 844 Size: 3469 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4845 1394 Size: 429 3022 3507 Fragments arranged by size: 3507 3022 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2683 3271 3439 4027 5453 5627 65 Size: 1183 1435 588 168 588 1426 174 1396 Fragments arranged by size: 1435 1426 1396 1183 588 588 174 168 BsrFI r'CCGG_y Cuts at: 1700 1810 5602 5789 1700 Size: 110 3792 187 2869 Fragments arranged by size: 3792 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6958 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6958 BssSI C'ACGA_G Cuts at: 3493 5018 6030 3493 Size: 1525 1012 4421 Fragments arranged by size: 4421 1525 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6958 BstBI TT'CG_AA Cuts at: 5762 5762 Size: 6958 BstEII G'GTnAC_C Cuts at: 1184 2500 1184 Size: 1316 5642 BstYI r'GATC_y Cuts at: 1972 2011 3738 4288 4655 5040 5057 6509 6595 Size: 39 1727 550 367 385 17 1452 86 Cuts at: 6595 6607 6953 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1727 1452 550 385 367 346 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2261 513 Size: 552 939 257 5210 Fragments arranged by size: 5210 939 552 257 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6219 Fragments arranged by size: 6219 683 56 BtsI GCAGTG_nn' Cuts at: 2480 3523 4474 5268 5288 2480 Size: 1043 951 794 20 4150 Fragments arranged by size: 4150 1043 951 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6958 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2665 2700 3253 3288 3421 3456 529 Size: 35 2101 35 553 35 133 35 4031 Fragments arranged by size: 4031 2101 553 133 35 35 35 35 DraI TTT'AAA Cuts at: 3091 4262 3091 Size: 1171 5787 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5965 809 Size: 507 4649 1802 Fragments arranged by size: 4649 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2146 4405 5294 Size: 54 1425 50 275 18 316 2259 889 Cuts at: 5294 6923 8 Size: 1629 43 Fragments arranged by size: 2259 1629 1425 889 316 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4405 1537 Size: 275 2593 4090 Fragments arranged by size: 4090 2593 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2355 2601 4888 1353 Size: 1002 246 2287 3423 Fragments arranged by size: 3423 2287 1002 246 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5545 5919 6065 387 Size: 1064 138 3956 374 146 1280 Fragments arranged by size: 3956 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3966 3966 Size: 6958 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2355 2376 2586 2760 2764 2844 Size: 393 375 348 21 210 174 4 80 Cuts at: 2844 2928 3180 3268 3432 3436 3684 3688 3768 Size: 84 252 88 164 4 248 4 80 Cuts at: 3768 4104 4192 5021 5618 6405 6673 1239 Size: 336 88 829 597 787 268 1524 Fragments arranged by size: 1524 829 787 597 393 375 348 336 268 252 248 210 174 164 88 88 84 80 80 21 4 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 3118 3118 Size: 6958 EcoO109I rG'GnC_Cy Cuts at: 2534 2958 3207 2534 Size: 424 249 6285 Fragments arranged by size: 6285 424 249 EcoRI G'AATT_C Cuts at: 2638 2814 2898 2982 3486 3654 3822 3990 4158 Size: 176 84 84 504 168 168 168 168 Cuts at: 4158 4267 4645 2638 Size: 109 378 4951 Fragments arranged by size: 4951 504 378 176 168 168 168 168 109 84 84 EcoRV GAT'ATC Cuts at: 4214 4214 Size: 6958 FspI TGC'GCA Cuts at: 5464 5464 Size: 6958 HaeII r_GCGC'y Cuts at: 1678 6105 1678 Size: 4427 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2861 2893 2947 Size: 32 135 32 431 32 826 32 54 Cuts at: 2947 2979 3955 3987 4039 4051 4071 4083 4123 Size: 32 976 32 52 12 20 12 40 Cuts at: 4123 4155 4231 4263 5599 5631 5673 5705 1373 Size: 32 76 32 1336 32 42 32 2626 Fragments arranged by size: 2626 1336 976 826 431 135 76 54 52 42 40 32 32 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 4302 4548 669 Size: 988 281 2364 246 3079 Fragments arranged by size: 3079 2364 988 281 246 HindIII A'AGCT_T Cuts at: 748 3588 3615 748 Size: 2840 27 4091 Fragments arranged by size: 4091 2840 27 HpaI GTT'AAC Cuts at: 4548 4548 Size: 6958 KpnI G_GTAC'C Cuts at: 2295 4283 2295 Size: 1988 4970 MfeI C'AATT_G Cuts at: 4557 4557 Size: 6958 MluI A'CGCG_T Cuts at: 6834 6834 Size: 6958 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2442 6072 6256 1305 Size: 1137 3630 184 2007 Fragments arranged by size: 3630 2007 1137 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2148 10 Size: 54 1425 343 316 4820 Fragments arranged by size: 4820 1425 343 316 54 MslI CAynn'nnrTG Cuts at: 518 1166 2839 2923 3243 3690 4916 5275 5434 Size: 648 1673 84 320 447 1226 359 159 Cuts at: 5434 518 Size: 2042 Fragments arranged by size: 2042 1673 1226 648 447 359 320 159 84 MspA1I CmG'CkG Cuts at: 1606 2137 4374 5051 6199 6444 1606 Size: 531 2237 677 1148 245 2120 Fragments arranged by size: 2237 2120 1148 677 531 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2261 513 Size: 552 939 257 5210 Fragments arranged by size: 5210 939 552 257 NdeI CA'TA_TG Cuts at: 387 3231 387 Size: 2844 4114 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6958 NotI GC'GGCC_GC Cuts at: 4405 4405 Size: 6958 NspI r_CATG'y Cuts at: 1132 1229 4316 5861 1132 Size: 97 3087 1545 2229 Fragments arranged by size: 3087 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6958 PciI A'CATG_T Cuts at: 5857 5857 Size: 6958 PflMI CCAn_nnn'nTGG Cuts at: 1108 3052 1108 Size: 1944 5014 PmeI GTTT'AAAC Cuts at: 4262 4262 Size: 6958 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5027 5059 6540 6572 5027 Size: 32 1481 32 5413 Fragments arranged by size: 5413 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2958 2958 Size: 6958 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6958 PsiI TTA'TAA Cuts at: 2801 4098 4528 2801 Size: 1297 430 5231 Fragments arranged by size: 5231 1297 430 PspOMI G'GGCC_C Cuts at: 3206 3206 Size: 6958 PstI C_TGCA'G Cuts at: 830 4310 830 Size: 3480 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6958 PvuII CAG'CTG Cuts at: 2137 2137 Size: 6958 SacI G_AGCT'C Cuts at: 3968 3968 Size: 6958 SalI G'TCGA_C Cuts at: 1936 4300 1936 Size: 2364 4594 SapI GCTCTTCn'nnn_ Cuts at: 2355 2355 Size: 6958 SbfI CC_TGCA'GG Cuts at: 4310 4310 Size: 6958 ScaI AGT'ACT Cuts at: 5206 6918 5206 Size: 1712 5246 SexAI A'CCwGG_T Cuts at: 2193 2193 Size: 6958 SfcI C'TryA_G Cuts at: 826 1045 2202 4306 5441 6122 6313 826 Size: 219 1157 2104 1135 681 191 1471 Fragments arranged by size: 2104 1471 1157 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6958 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6958 SmaI CCC'GGG Cuts at: 1872 4285 1872 Size: 2413 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3579 5062 5963 6225 820 Size: 197 926 1636 1483 901 262 1553 Fragments arranged by size: 1636 1553 1483 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4749 493 Size: 4256 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6958 SphI G_CATG'C Cuts at: 1132 4316 1132 Size: 3184 3774 SspI AAT'ATT Cuts at: 5 52 4882 5 Size: 47 4830 2081 Fragments arranged by size: 4830 2081 47 StuI AGG'CCT Cuts at: 1517 3197 3449 1517 Size: 1680 252 5026 Fragments arranged by size: 5026 1680 252 StyI C'CwwG_G Cuts at: 513 1065 2004 2041 2261 4397 513 Size: 552 939 37 220 2136 3074 Fragments arranged by size: 3074 2136 939 552 220 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4985 5002 5155 5340 5785 325 Size: 1255 3405 17 153 185 445 1498 Fragments arranged by size: 3405 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5204 6916 96 Size: 274 80 33 51 1443 3227 1712 138 Fragments arranged by size: 3227 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6918 6950 6918 Size: 32 6926 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6958 XbaI T'CTAG_A Cuts at: 4294 4294 Size: 6958 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6958 XmaI C'CCGG_G Cuts at: 1870 4283 1870 Size: 2413 4545 XmnI GAAnn'nnTTC Cuts at: 3281 3654 3701 4162 5087 3281 Size: 373 47 461 925 5152 Fragments arranged by size: 5152 925 461 373 47 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4766 276 Size: 53 83 186 936 3232 2468 Fragments arranged by size: 3232 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI