(Circular) (Six-base) MAPSORT of: pFN21AB6236.seq Check: 4510 from: 1 to: 6733 pFN21AB6236 6733 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4543 278 Size: 53 83 186 936 3007 2468 Fragments arranged by size: 3007 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2174 4076 1937 Size: 237 1902 4594 Fragments arranged by size: 4594 1902 237 Acc65I G'GTAC_C Cuts at: 4054 4054 Size: 6733 AclI AA'CG_TT Cuts at: 1563 4860 5233 1563 Size: 3297 373 3063 Fragments arranged by size: 3297 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3128 3593 3847 4796 6180 6448 1632 Size: 1496 465 254 949 1384 268 1917 Fragments arranged by size: 1917 1496 1384 949 465 268 254 AfeI AGC'GCT Cuts at: 2285 2285 Size: 6733 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6536 AflIII A'CryG_T Cuts at: 1203 5632 6609 1203 Size: 4429 977 1327 Fragments arranged by size: 4429 1327 977 AgeI A'CCGG_T Cuts at: 5564 5564 Size: 6733 AhdI GACnn_n'nnGTC Cuts at: 3598 5462 3598 Size: 1864 4869 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6733 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2822 1184 Size: 1638 5095 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3257 3289 3257 Size: 32 6701 AlwNI CAG_nnn'CTG Cuts at: 2249 2930 3571 4476 6048 2249 Size: 681 641 905 1572 2934 Fragments arranged by size: 2934 1572 905 681 641 ApaLI G'TGCA_C Cuts at: 2807 3181 4790 5946 2807 Size: 374 1609 1156 3594 Fragments arranged by size: 3594 1609 1156 374 ApoI r'AATT_y Cuts at: 1493 4010 4042 4268 4420 6529 1493 Size: 2517 32 226 152 2109 1697 Fragments arranged by size: 2517 2109 1697 226 152 32 AscI GG'CGCG_CC Cuts at: 3929 3929 Size: 6733 AseI AT'TA_AT Cuts at: 160 1032 5287 160 Size: 872 4255 1606 Fragments arranged by size: 4255 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6733 AvaI C'yCGr_G Cuts at: 1870 1943 4058 1870 Size: 73 2115 4545 Fragments arranged by size: 4545 2115 73 BamHI G'GATC_C Cuts at: 4063 4430 4063 Size: 367 6366 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2633 3533 4054 5509 Size: 325 217 627 221 625 900 521 1455 Cuts at: 5509 618 Size: 1842 Fragments arranged by size: 1842 1455 900 627 625 521 325 221 217 BanII G_rGCy'C Cuts at: 1391 2051 2726 4052 1391 Size: 660 675 1326 4072 Fragments arranged by size: 4072 1326 675 660 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 6733 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2796 2966 928 Size: 971 897 170 4695 Fragments arranged by size: 4695 971 897 170 BbvCI CC'TCA_GC Cuts at: 2868 2868 Size: 6733 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4924 4958 1903 Size: 34 2987 34 3678 Fragments arranged by size: 3678 2987 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2169 4625 5835 2169 Size: 2456 1210 3067 Fragments arranged by size: 3067 2456 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6733 BfrBI ATG'CAT Cuts at: 2604 2604 Size: 6733 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3137 5344 136 Size: 107 122 71 631 605 1465 2207 1525 Fragments arranged by size: 2207 1525 1465 631 605 122 107 71 BglII A'GATC_T Cuts at: 3012 6728 3012 Size: 3716 3017 BlpI GC'TnA_GC Cuts at: 2111 4150 2111 Size: 2039 4694 Bme1580I G_kGCm'C Cuts at: 1790 2811 3185 4794 5950 1790 Size: 1021 374 1609 1156 2573 Fragments arranged by size: 2573 1609 1156 1021 374 BmgBI CAC'GTC Cuts at: 1318 2377 3175 1318 Size: 1059 798 4876 Fragments arranged by size: 4876 1059 798 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4464 5422 442 Size: 416 53 83 399 24 3047 958 1753 Fragments arranged by size: 3047 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6733 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2065 2065 Size: 6733 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 3761 5393 1239 Size: 1151 1371 1632 2579 Fragments arranged by size: 2579 1632 1371 1151 Bpu10I CC'TnA_GC Cuts at: 2868 2868 Size: 6733 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3128 4858 5723 6021 3128 Size: 1730 865 298 3840 Fragments arranged by size: 3840 1730 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5396 882 Size: 617 3897 2219 Fragments arranged by size: 3897 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4524 493 Size: 711 3320 2702 Fragments arranged by size: 3320 2702 711 BsaBI GATnn'nnATC Cuts at: 6727 6727 Size: 6733 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2184 4540 4922 Size: 53 83 186 936 476 175 2356 382 Cuts at: 4922 5557 275 Size: 635 1451 Fragments arranged by size: 2356 1451 936 635 476 382 186 175 83 53 BsaWI w'CCGG_w Cuts at: 2963 5166 5564 5838 5985 6396 2963 Size: 2203 398 274 147 411 3300 Fragments arranged by size: 3300 2203 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2231 2261 2903 2933 1178 Size: 30 165 30 828 30 642 30 4978 Fragments arranged by size: 4978 828 642 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2252 2911 3132 1186 Size: 1066 659 221 4787 Fragments arranged by size: 4787 1066 659 221 BseYI C'CCAG_C Cuts at: 2044 2972 3775 5936 6432 2044 Size: 928 803 2161 496 2345 Fragments arranged by size: 2345 2161 928 803 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2159 3232 1163 Size: 573 423 1073 4664 Fragments arranged by size: 4664 1073 573 423 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4183 4944 5972 1540 Size: 275 186 2182 761 1028 2301 Fragments arranged by size: 2301 2182 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2811 3185 4020 4794 4879 5950 2811 Size: 374 835 774 85 1071 3594 Fragments arranged by size: 3594 1071 835 774 374 85 BsmI GAATG_Cn' Cuts at: 3980 4244 4337 3980 Size: 264 93 6376 Fragments arranged by size: 6376 264 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2726 2811 3185 4020 4052 4794 Size: 399 261 675 85 374 835 32 742 Cuts at: 4794 4879 5950 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 835 742 675 399 374 261 85 85 32 BspEI T'CCGG_A Cuts at: 6396 6396 Size: 6733 BspHI T'CATG_A Cuts at: 4622 6352 4622 Size: 1730 5003 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2334 4088 844 Size: 1490 1754 3489 Fragments arranged by size: 3489 1754 1490 BsrBI CCG'CTC Cuts at: 1394 1823 4620 1394 Size: 429 2797 3507 Fragments arranged by size: 3507 2797 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2582 5228 5402 65 Size: 1183 1334 2646 174 1396 Fragments arranged by size: 2646 1396 1334 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5377 5564 1700 Size: 110 3567 187 2869 Fragments arranged by size: 3567 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6733 BssHII G'CGCG_C Cuts at: 1925 3929 1925 Size: 2004 4729 BssSI C'ACGA_G Cuts at: 4793 5805 4793 Size: 1012 5721 BstAPI GCAn_nnn'nTGC Cuts at: 794 3033 3528 794 Size: 2239 495 3999 Fragments arranged by size: 3999 2239 495 BstBI TT'CG_AA Cuts at: 5537 5537 Size: 6733 BstEII G'GTnAC_C Cuts at: 1184 2254 1184 Size: 1070 5663 BstXI CCAn_nnnn'nTGG Cuts at: 3688 3775 3688 Size: 87 6646 BstYI r'GATC_y Cuts at: 1972 3012 3558 4063 4430 4815 4832 6284 6370 Size: 1040 546 505 367 385 17 1452 86 Cuts at: 6370 6382 6728 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1040 546 505 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2334 2334 Size: 6733 BtgI C'CryG_G Cuts at: 513 1065 2004 2259 2437 2637 3186 3649 3966 Size: 552 939 255 178 200 549 463 317 Cuts at: 3966 513 Size: 3280 Fragments arranged by size: 3280 939 552 549 463 317 255 200 178 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2008 487 Size: 683 56 782 5212 Fragments arranged by size: 5212 782 683 56 BtsI GCAGTG_nn' Cuts at: 2407 2740 4249 5043 5063 2407 Size: 333 1509 794 20 4077 Fragments arranged by size: 4077 1509 794 333 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6733 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2482 2517 529 Size: 35 1918 35 4745 Fragments arranged by size: 4745 1918 35 35 DraI TTT'AAA Cuts at: 4037 4037 Size: 6733 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5740 809 Size: 507 4424 1802 Fragments arranged by size: 4424 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2277 2640 2832 Size: 54 1425 50 275 18 447 363 192 Cuts at: 2832 3457 3634 4180 5069 6698 8 Size: 625 177 546 889 1629 43 Fragments arranged by size: 1629 1425 889 625 546 447 363 275 192 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4180 1537 Size: 275 2368 4090 Fragments arranged by size: 4090 2368 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3677 4663 1353 Size: 2324 986 3423 Fragments arranged by size: 3423 2324 986 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5320 5694 5840 387 Size: 1064 138 3731 374 146 1280 Fragments arranged by size: 3731 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2390 3128 3593 3761 3847 4796 5393 Size: 393 758 738 465 168 86 949 597 Cuts at: 5393 6180 6448 1239 Size: 787 268 1524 Fragments arranged by size: 1524 949 787 758 738 597 465 393 268 168 86 EcoNI CCTnn'n_nnAGG Cuts at: 3146 3146 Size: 6733 EcoO109I rG'GnC_Cy Cuts at: 2545 3000 3064 3139 3304 3421 3568 3610 2545 Size: 455 64 75 165 117 147 42 5668 Fragments arranged by size: 5668 455 165 147 117 75 64 42 EcoRI G'AATT_C Cuts at: 4042 4420 4042 Size: 378 6355 FspI TGC'GCA Cuts at: 2021 2399 5239 2021 Size: 378 2840 3515 Fragments arranged by size: 3515 2840 378 HaeII r_GCGC'y Cuts at: 1678 2012 2111 2287 5880 1678 Size: 334 99 176 3593 2531 Fragments arranged by size: 3593 2531 334 176 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2143 2156 2175 2188 2231 Size: 32 135 32 571 13 19 13 43 Cuts at: 2231 2263 2903 2935 3584 3616 5374 5406 5448 Size: 32 640 32 649 32 1758 32 42 Cuts at: 5448 5480 1373 Size: 32 2626 Fragments arranged by size: 2626 1758 649 640 571 135 43 42 32 32 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 2175 4077 4323 669 Size: 988 281 237 1902 246 3079 Fragments arranged by size: 3079 1902 988 281 246 237 HindIII A'AGCT_T Cuts at: 748 2858 748 Size: 2110 4623 HpaI GTT'AAC Cuts at: 4323 4323 Size: 6733 KasI G'GCGC_C Cuts at: 2008 2008 Size: 6733 KpnI G_GTAC'C Cuts at: 4058 4058 Size: 6733 MfeI C'AATT_G Cuts at: 4332 4332 Size: 6733 MluI A'CGCG_T Cuts at: 6609 6609 Size: 6733 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3426 3831 5847 6031 1305 Size: 2121 405 2016 184 2007 Fragments arranged by size: 2121 2016 2007 405 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2834 3459 3636 10 Size: 54 1425 343 1002 625 177 3107 Fragments arranged by size: 3107 1425 1002 625 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2193 2687 2804 3654 3686 4691 5050 Size: 648 1027 494 117 850 32 1005 359 Cuts at: 5050 5209 518 Size: 159 2042 Fragments arranged by size: 2042 1027 1005 850 648 494 359 159 117 32 MspA1I CmG'CkG Cuts at: 1606 2439 2639 3368 3711 4149 4826 5974 6219 Size: 833 200 729 343 438 677 1148 245 Cuts at: 6219 1606 Size: 2120 Fragments arranged by size: 2120 1148 833 729 677 438 343 245 200 NarI GG'CG_CC Cuts at: 2009 2009 Size: 6733 NcoI C'CATG_G Cuts at: 513 1065 2004 3186 3649 3966 513 Size: 552 939 1182 463 317 3280 Fragments arranged by size: 3280 1182 939 552 463 317 NdeI CA'TA_TG Cuts at: 387 387 Size: 6733 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6733 NotI GC'GGCC_GC Cuts at: 4180 4180 Size: 6733 NsiI A_TGCA'T Cuts at: 2606 2606 Size: 6733 NspI r_CATG'y Cuts at: 1132 1229 2692 2986 4091 5636 1132 Size: 97 1463 294 1105 1545 2229 Fragments arranged by size: 2229 1545 1463 1105 294 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6733 PciI A'CATG_T Cuts at: 5632 5632 Size: 6733 PflMI CCAn_nnn'nTGG Cuts at: 1108 3825 1108 Size: 2717 4016 PfoI T'CCnGG_A Cuts at: 2367 2900 3321 3738 4023 2367 Size: 533 421 417 285 5077 Fragments arranged by size: 5077 533 421 417 285 PmeI GTTT'AAAC Cuts at: 4037 4037 Size: 6733 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2402 2434 4802 4834 6315 6347 2402 Size: 32 2368 32 1481 32 2788 Fragments arranged by size: 2788 2368 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3000 3064 3304 3421 3610 3000 Size: 64 240 117 189 6123 Fragments arranged by size: 6123 240 189 117 64 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6733 PsiI TTA'TAA Cuts at: 4303 4303 Size: 6733 PstI C_TGCA'G Cuts at: 830 2058 2331 3390 4085 830 Size: 1228 273 1059 695 3478 Fragments arranged by size: 3478 1228 1059 695 273 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6733 SacII CC_GC'GG Cuts at: 2440 2640 2440 Size: 200 6533 SalI G'TCGA_C Cuts at: 1936 2173 4075 1936 Size: 237 1902 4594 Fragments arranged by size: 4594 1902 237 SbfI CC_TGCA'GG Cuts at: 2058 4085 2058 Size: 2027 4706 ScaI AGT'ACT Cuts at: 3125 4981 6693 3125 Size: 1856 1712 3165 Fragments arranged by size: 3165 1856 1712 SfcI C'TryA_G Cuts at: 826 1045 2054 2327 3386 3563 3892 3940 4081 Size: 219 1009 273 1059 177 329 48 141 Cuts at: 4081 5216 5897 6088 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1059 1009 681 329 273 219 191 177 141 48 SfoI GGC'GCC Cuts at: 2010 2010 Size: 6733 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6733 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6733 SmaI CCC'GGG Cuts at: 1872 4060 1872 Size: 2188 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3143 4837 5738 6000 820 Size: 197 926 1200 1694 901 262 1553 Fragments arranged by size: 1694 1553 1200 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4524 493 Size: 4031 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6733 SphI G_CATG'C Cuts at: 1132 4091 1132 Size: 2959 3774 SspI AAT'ATT Cuts at: 5 52 4657 5 Size: 47 4605 2081 Fragments arranged by size: 4605 2081 47 StuI AGG'CCT Cuts at: 1517 2873 1517 Size: 1356 5377 StyI C'CwwG_G Cuts at: 513 1065 2004 2340 3186 3261 3649 3720 3729 Size: 552 939 336 846 75 388 71 9 Cuts at: 3729 3966 4172 513 Size: 237 206 3074 Fragments arranged by size: 3074 939 846 552 388 336 237 206 75 71 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2252 2903 3251 3898 4760 4777 4930 Size: 1255 672 651 348 647 862 17 153 Cuts at: 4930 5115 5560 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 862 672 651 647 445 348 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3123 3576 4979 Size: 274 80 33 51 1443 1146 453 1403 Cuts at: 4979 6691 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1403 1146 453 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6693 6725 6693 Size: 32 6701 Tth111I GACn'n_nGTC Cuts at: 1316 2537 1316 Size: 1221 5512 XbaI T'CTAG_A Cuts at: 4069 4069 Size: 6733 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3736 3736 Size: 6733 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6733 XmaI C'CCGG_G Cuts at: 1870 4058 1870 Size: 2188 4545 XmnI GAAnn'nnTTC Cuts at: 3500 4862 3500 Size: 1362 5371 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4541 276 Size: 53 83 186 936 3007 2468 Fragments arranged by size: 3007 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AvrII BaeI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI