(Circular) (Six-base) MAPSORT of: pFN21AB6225.seq Check: 6025 from: 1 to: 6718 pFN21AB6225 6718 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3526 3526 Size: 6718 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4528 278 Size: 53 83 186 936 2992 2468 Fragments arranged by size: 2992 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2703 3075 3225 4061 1937 Size: 766 372 150 836 4594 Fragments arranged by size: 4594 836 766 372 150 Acc65I G'GTAC_C Cuts at: 2442 4039 2442 Size: 1597 5121 AclI AA'CG_TT Cuts at: 1563 3989 4845 5218 1563 Size: 2426 856 373 3063 Fragments arranged by size: 3063 2426 856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2628 4781 6165 6433 1632 Size: 996 2153 1384 268 1917 Fragments arranged by size: 2153 1917 1384 996 268 AfeI AGC'GCT Cuts at: 2354 2354 Size: 6718 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6521 AflIII A'CryG_T Cuts at: 1203 5617 6594 1203 Size: 4414 977 1327 Fragments arranged by size: 4414 1327 977 AgeI A'CCGG_T Cuts at: 5549 5549 Size: 6718 AhdI GACnn_n'nnGTC Cuts at: 5447 5447 Size: 6718 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6718 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2568 1184 Size: 1384 5334 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3540 3572 3540 Size: 32 6686 AlwNI CAG_nnn'CTG Cuts at: 2255 2439 2740 3364 4461 6033 2255 Size: 184 301 624 1097 1572 2940 Fragments arranged by size: 2940 1572 1097 624 301 184 ApaI G_GGCC'C Cuts at: 2015 2382 2932 2015 Size: 367 550 5801 Fragments arranged by size: 5801 550 367 ApaLI G'TGCA_C Cuts at: 4775 5931 4775 Size: 1156 5562 ApoI r'AATT_y Cuts at: 1493 3980 4027 4253 4405 6514 1493 Size: 2487 47 226 152 2109 1697 Fragments arranged by size: 2487 2109 1697 226 152 47 AseI AT'TA_AT Cuts at: 160 1032 5272 160 Size: 872 4240 1606 Fragments arranged by size: 4240 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6718 AvaI C'yCGr_G Cuts at: 1870 1943 2094 3090 3783 4043 1870 Size: 73 151 996 693 260 4545 Fragments arranged by size: 4545 996 693 260 151 73 AvrII C'CTAG_G Cuts at: 3055 3055 Size: 6718 BamHI G'GATC_C Cuts at: 4048 4415 4048 Size: 367 6351 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2107 2442 2917 3504 3755 Size: 325 217 627 320 335 475 587 251 Cuts at: 3755 4039 5494 618 Size: 284 1455 1842 Fragments arranged by size: 1842 1455 627 587 475 335 325 320 284 251 217 BanII G_rGCy'C Cuts at: 1391 2015 2040 2382 2542 2912 2932 2975 4037 Size: 624 25 342 160 370 20 43 1062 Cuts at: 4037 1391 Size: 4072 Fragments arranged by size: 4072 1062 624 370 342 160 43 25 20 BbeI G_GCGC'C Cuts at: 2921 3508 2921 Size: 587 6131 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2079 3894 928 Size: 971 180 1815 3752 Fragments arranged by size: 3752 1815 971 180 BbvCI CC'TCA_GC Cuts at: 2962 3643 2962 Size: 681 6037 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3524 3558 4909 4943 1903 Size: 34 50 34 1503 34 1351 34 3678 Fragments arranged by size: 3678 1503 1351 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4610 5820 4610 Size: 1210 5508 BclI T'GATC_A Cuts at: 1548 1548 Size: 6718 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2957 3282 5329 Size: 107 122 71 631 605 1285 325 2047 Cuts at: 5329 136 Size: 1525 Fragments arranged by size: 2047 1525 1285 631 605 325 122 107 71 BglII A'GATC_T Cuts at: 6713 6713 Size: 6718 BlpI GC'TnA_GC Cuts at: 2039 2849 3619 4135 2039 Size: 810 770 516 4622 Fragments arranged by size: 4622 810 770 516 Bme1580I G_kGCm'C Cuts at: 1790 2015 2112 2382 2932 3500 4779 5935 1790 Size: 225 97 270 550 568 1279 1156 2573 Fragments arranged by size: 2573 1279 1156 568 550 270 225 97 BmgBI CAC'GTC Cuts at: 1318 3591 1318 Size: 2273 4445 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2766 3382 4449 Size: 416 53 83 399 24 1349 616 1067 Cuts at: 4449 5407 442 Size: 958 1753 Fragments arranged by size: 1753 1349 1067 958 616 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6718 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2821 2821 Size: 6718 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2664 2849 2959 2989 3278 3386 3431 Size: 815 610 185 110 30 289 108 45 Cuts at: 3431 3917 5378 1239 Size: 486 1461 2579 Fragments arranged by size: 2579 1461 815 610 486 289 185 110 108 45 30 Bpu10I CC'TnA_GC Cuts at: 2962 3333 3643 2962 Size: 371 310 6037 Fragments arranged by size: 6037 371 310 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3149 4843 5708 6006 3149 Size: 1694 865 298 3861 Fragments arranged by size: 3861 1694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2514 2879 3087 5381 882 Size: 617 1015 365 208 2294 2219 Fragments arranged by size: 2294 2219 1015 617 365 208 BsaAI yAC'GTr Cuts at: 493 1204 2322 3532 4509 493 Size: 711 1118 1210 977 2702 Fragments arranged by size: 2702 1210 1118 977 711 BsaBI GATnn'nnATC Cuts at: 6712 6712 Size: 6718 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2689 2918 3505 4525 Size: 53 83 186 936 1156 229 587 1020 Cuts at: 4525 4907 5542 275 Size: 382 635 1451 Fragments arranged by size: 1451 1156 1020 936 635 587 382 229 186 83 53 BsaWI w'CCGG_w Cuts at: 2118 2445 2469 5151 5549 5823 5970 6381 2118 Size: 327 24 2682 398 274 147 411 2455 Fragments arranged by size: 2682 2455 411 398 327 274 147 24 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3366 3396 3540 3570 3620 Size: 30 165 30 1963 30 144 30 50 Cuts at: 3620 3650 3805 3835 1178 Size: 30 155 30 4061 Fragments arranged by size: 4061 1963 165 155 144 50 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2522 3808 1186 Size: 1336 1286 4096 Fragments arranged by size: 4096 1336 1286 BseYI C'CCAG_C Cuts at: 2033 2214 2295 2355 2541 2595 2808 3124 3579 Size: 181 81 60 186 54 213 316 455 Cuts at: 3579 3632 3658 3791 5921 6417 2033 Size: 53 26 133 2130 496 2334 Fragments arranged by size: 2334 2130 496 455 316 213 186 181 133 81 60 54 53 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3554 1163 Size: 573 1818 4327 Fragments arranged by size: 4327 1818 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2952 3277 4168 4929 5957 1540 Size: 275 186 951 325 891 761 1028 2301 Fragments arranged by size: 2301 1028 951 891 761 325 275 186 BsiHKAI G_wGCw'C Cuts at: 2685 2912 2975 3270 3444 4779 4864 5935 2685 Size: 227 63 295 174 1335 85 1071 3468 Fragments arranged by size: 3468 1335 1071 295 227 174 85 63 BsmI GAATG_Cn' Cuts at: 2648 4229 4322 2648 Size: 1581 93 5044 Fragments arranged by size: 5044 1581 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3597 3597 Size: 6718 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2040 2112 2382 2542 2685 2912 Size: 399 225 25 72 270 160 143 227 Cuts at: 2912 2932 2975 3270 3444 3500 4037 4779 4864 Size: 20 43 295 174 56 537 742 85 Cuts at: 4864 5935 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 537 399 295 270 227 225 174 160 143 85 72 56 43 25 20 BspEI T'CCGG_A Cuts at: 2118 6381 2118 Size: 4263 2455 BspHI T'CATG_A Cuts at: 4607 6337 4607 Size: 1730 4988 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2560 2880 3526 4073 844 Size: 1716 320 646 547 3489 Fragments arranged by size: 3489 1716 646 547 320 BsrBI CCG'CTC Cuts at: 1394 1823 2394 3618 4605 1394 Size: 429 571 1224 987 3507 Fragments arranged by size: 3507 1224 987 571 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2188 2403 5213 5387 65 Size: 1183 940 215 2810 174 1396 Fragments arranged by size: 2810 1396 1183 940 215 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2914 2951 5362 5549 1700 Size: 110 1104 37 2411 187 2869 Fragments arranged by size: 2869 2411 1104 187 110 37 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6718 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6718 BssSI C'ACGA_G Cuts at: 3543 4778 5790 3543 Size: 1235 1012 4471 Fragments arranged by size: 4471 1235 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6718 BstBI TT'CG_AA Cuts at: 5522 5522 Size: 6718 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6718 BstXI CCAn_nnnn'nTGG Cuts at: 3676 3752 3676 Size: 76 6642 BstYI r'GATC_y Cuts at: 1972 2283 3327 4048 4415 4800 4817 6269 6355 Size: 311 1044 721 367 385 17 1452 86 Cuts at: 6355 6367 6713 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1044 721 385 367 346 311 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2382 3271 513 Size: 552 1317 889 3960 Fragments arranged by size: 3960 1317 889 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3875 487 Size: 683 56 2649 3330 Fragments arranged by size: 3330 2649 683 56 BtsI GCAGTG_nn' Cuts at: 3941 4234 5028 5048 3941 Size: 293 794 20 5611 Fragments arranged by size: 5611 794 293 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6718 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6683 DraI TTT'AAA Cuts at: 4022 4022 Size: 6718 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2722 5725 809 Size: 507 1406 3003 1802 Fragments arranged by size: 3003 1802 1406 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 2246 2841 Size: 54 1425 50 275 18 232 184 595 Cuts at: 2841 2949 3274 4165 5054 6683 8 Size: 108 325 891 889 1629 43 Fragments arranged by size: 1629 1425 891 889 595 325 275 232 184 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2949 3274 4165 1537 Size: 275 1137 325 891 4090 Fragments arranged by size: 4090 1137 891 325 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2604 2715 3132 3140 4648 1353 Size: 1251 111 417 8 1508 3423 Fragments arranged by size: 3423 1508 1251 417 111 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2995 5305 5679 5825 387 Size: 1064 138 1406 2310 374 146 1280 Fragments arranged by size: 2310 1406 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2910 2973 2910 Size: 63 6655 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2628 2664 2849 2959 2989 3278 Size: 393 422 574 36 185 110 30 289 Cuts at: 3278 3386 3431 3917 4781 5378 6165 6433 1239 Size: 108 45 486 864 597 787 268 1524 Fragments arranged by size: 1524 864 787 597 574 486 422 393 289 268 185 110 108 45 36 30 EcoO109I rG'GnC_Cy Cuts at: 2011 2252 2869 2929 3406 3788 3813 3876 3972 Size: 241 617 60 477 382 25 63 96 Cuts at: 3972 2011 Size: 4757 Fragments arranged by size: 4757 617 477 382 241 96 63 60 25 EcoRI G'AATT_C Cuts at: 4027 4405 4027 Size: 378 6340 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3190 3190 Size: 6718 FseI GG_CCGG'CC Cuts at: 2955 2955 Size: 6718 FspI TGC'GCA Cuts at: 3653 5224 3653 Size: 1571 5147 HaeII r_GCGC'y Cuts at: 1678 2356 2856 2921 3508 5865 1678 Size: 678 500 65 587 2357 2531 Fragments arranged by size: 2531 2357 678 587 500 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3429 3461 3733 3765 3805 Size: 32 135 32 1857 32 272 32 40 Cuts at: 3805 3837 5359 5391 5433 5465 1373 Size: 32 1522 32 42 32 2626 Fragments arranged by size: 2626 1857 1522 272 135 42 40 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2425 4062 4308 669 Size: 988 281 487 1637 246 3079 Fragments arranged by size: 3079 1637 988 487 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6718 HpaI GTT'AAC Cuts at: 4308 4308 Size: 6718 KasI G'GCGC_C Cuts at: 2917 3504 2917 Size: 587 6131 KpnI G_GTAC'C Cuts at: 2446 4043 2446 Size: 1597 5121 MfeI C'AATT_G Cuts at: 2880 4317 2880 Size: 1437 5281 MluI A'CGCG_T Cuts at: 6594 6594 Size: 6718 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2157 3041 5832 6016 1305 Size: 852 884 2791 184 2007 Fragments arranged by size: 2791 2007 884 852 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2064 2248 10 Size: 54 1425 343 232 184 4480 Fragments arranged by size: 4480 1425 343 232 184 54 MslI CAynn'nnrTG Cuts at: 518 1166 2819 4676 5035 5194 518 Size: 648 1653 1857 359 159 2042 Fragments arranged by size: 2042 1857 1653 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2491 2779 2941 3608 3662 4134 4811 5959 Size: 885 288 162 667 54 472 677 1148 Cuts at: 5959 6204 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 885 677 667 472 288 245 162 54 NaeI GCC'GGC Cuts at: 2916 2953 2916 Size: 37 6681 NarI GG'CG_CC Cuts at: 2918 3505 2918 Size: 587 6131 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6166 NdeI CA'TA_TG Cuts at: 387 387 Size: 6718 NgoMIV G'CCGG_C Cuts at: 2914 2951 2914 Size: 37 6681 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6718 NotI GC'GGCC_GC Cuts at: 4165 4165 Size: 6718 NspI r_CATG'y Cuts at: 1132 1229 2401 2990 4076 5621 1132 Size: 97 1172 589 1086 1545 2229 Fragments arranged by size: 2229 1545 1172 1086 589 97 PasI CC'CwG_GG Cuts at: 1348 2255 1348 Size: 907 5811 PciI A'CATG_T Cuts at: 5617 5617 Size: 6718 PflMI CCAn_nnn'nTGG Cuts at: 1108 2255 2439 3031 3364 3802 1108 Size: 1147 184 592 333 438 4024 Fragments arranged by size: 4024 1147 592 438 333 184 PfoI T'CCnGG_A Cuts at: 2726 3965 2726 Size: 1239 5479 PmeI GTTT'AAAC Cuts at: 4022 4022 Size: 6718 PmlI CAC'GTG Cuts at: 2322 2322 Size: 6718 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3540 3572 4787 4819 6300 6332 3540 Size: 32 1215 32 1481 32 3926 Fragments arranged by size: 3926 1481 1215 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2869 3406 3788 3813 3972 2869 Size: 537 382 25 159 5615 Fragments arranged by size: 5615 537 382 159 25 PshAI GACnn'nnGTC Cuts at: 1613 2896 1613 Size: 1283 5435 PsiI TTA'TAA Cuts at: 4288 4288 Size: 6718 PspOMI G'GGCC_C Cuts at: 2011 2378 2928 2011 Size: 367 550 5801 Fragments arranged by size: 5801 550 367 PstI C_TGCA'G Cuts at: 830 2667 2745 3210 4070 830 Size: 1837 78 465 860 3478 Fragments arranged by size: 3478 1837 860 465 78 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6718 PvuII CAG'CTG Cuts at: 2491 3662 2491 Size: 1171 5547 SacI G_AGCT'C Cuts at: 2912 2975 2912 Size: 63 6655 SalI G'TCGA_C Cuts at: 1936 4060 1936 Size: 2124 4594 SbfI CC_TGCA'GG Cuts at: 4070 4070 Size: 6718 ScaI AGT'ACT Cuts at: 4966 6678 4966 Size: 1712 5006 SexAI A'CCwGG_T Cuts at: 3357 3357 Size: 6718 SfcI C'TryA_G Cuts at: 826 1045 2635 2663 2741 3206 4066 5201 5882 Size: 219 1590 28 78 465 860 1135 681 Cuts at: 5882 6073 826 Size: 191 1471 Fragments arranged by size: 1590 1471 1135 860 681 465 219 191 78 28 SfiI GGCCn_nnn'nGGCC Cuts at: 2957 2957 Size: 6718 SfoI GGC'GCC Cuts at: 2919 3506 2919 Size: 587 6131 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6718 SgrAI Cr'CCGG_yG Cuts at: 1700 2914 1700 Size: 1214 5504 SmaI CCC'GGG Cuts at: 1872 2096 4045 1872 Size: 224 1949 4545 Fragments arranged by size: 4545 1949 224 SmlI C'TyrA_G Cuts at: 820 1017 1943 3164 3783 4822 5723 5985 820 Size: 197 926 1221 619 1039 901 262 1553 Fragments arranged by size: 1553 1221 1039 926 901 619 262 197 SnaBI TAC'GTA Cuts at: 493 4509 493 Size: 4016 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6718 SphI G_CATG'C Cuts at: 1132 2401 4076 1132 Size: 1269 1675 3774 Fragments arranged by size: 3774 1675 1269 SspI AAT'ATT Cuts at: 5 52 4642 5 Size: 47 4590 2081 Fragments arranged by size: 4590 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6718 StyI C'CwwG_G Cuts at: 513 1065 2328 3055 3192 3508 4157 513 Size: 552 1263 727 137 316 649 3074 Fragments arranged by size: 3074 1263 727 649 552 316 137 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2318 2530 3737 3947 4745 4762 4915 Size: 1255 738 212 1207 210 798 17 153 Cuts at: 4915 5100 5545 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1207 798 738 445 212 210 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3390 3870 4964 Size: 274 80 33 51 1443 1413 480 1094 Cuts at: 4964 6676 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1413 1094 480 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2057 2089 2216 2248 3366 3398 3620 3652 3748 Size: 32 127 32 1118 32 222 32 96 Cuts at: 3748 3780 6678 6710 2057 Size: 32 2898 32 2065 Fragments arranged by size: 2898 2065 1118 222 127 96 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2708 3071 3221 1316 Size: 1392 363 150 4813 Fragments arranged by size: 4813 1392 363 150 XbaI T'CTAG_A Cuts at: 4054 4054 Size: 6718 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2776 3799 2776 Size: 1023 5695 XhoI C'TCGA_G Cuts at: 1943 3783 1943 Size: 1840 4878 XmaI C'CCGG_G Cuts at: 1870 2094 4043 1870 Size: 224 1949 4545 Fragments arranged by size: 4545 1949 224 XmnI GAAnn'nnTTC Cuts at: 4847 4847 Size: 6718 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4526 276 Size: 53 83 186 936 2992 2468 Fragments arranged by size: 2992 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SrfI SwaI