Restriction map for
pFN21AB6225
(Linear) (Six-base) MAPSORT of: KIBB6225.seq Check: 1000 from: 1 to: 2013
KIBB6225.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1521 2013
Size: 1521 492
AccI GT'mk_AC
Cuts at: 0 698 1070 1220 2013
Size: 698 372 150 793
Fragments arranged by size:
793 698 372 150
Acc65I G'GTAC_C
Cuts at: 0 437 2013
Size: 437 1576
AclI AA'CG_TT
Cuts at: 0 1984 2013
Size: 1984 29
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 2013
Size: 623 1390
AfeI AGC'GCT
Cuts at: 0 349 2013
Size: 349 1664
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 563 2013
Size: 563 1450
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1535 1567 2013
Size: 1535 32 446
Fragments arranged by size:
1535 446 32
AlwNI CAG_nnn'CTG
Cuts at: 0 250 434 735 1359 2013
Size: 250 184 301 624 654
Fragments arranged by size:
654 624 301 250 184
ApaI G_GGCC'C
Cuts at: 0 10 377 927 2013
Size: 10 367 550 1086
Fragments arranged by size:
1086 550 367 10
ApoI r'AATT_y
Cuts at: 0 1975 2013
Size: 1975 38
AvaI C'yCGr_G
Cuts at: 0 89 1085 1778 2013
Size: 89 996 693 235
Fragments arranged by size:
996 693 235 89
AvrII C'CTAG_G
Cuts at: 0 1050 2013
Size: 1050 963
BanI G'GyrC_C
Cuts at: 0 102 437 912 1499 1750 2013
Size: 102 335 475 587 251 263
Fragments arranged by size:
587 475 335 263 251 102
BanII G_rGCy'C
Cuts at: 0 10 35 377 537 907 927 970 2013
Size: 10 25 342 160 370 20 43 1043
Fragments arranged by size:
1043 370 342 160 43 25 20 10
BbeI G_GCGC'C
Cuts at: 0 916 1503 2013
Size: 916 587 510
Fragments arranged by size:
916 587 510
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 1889 2013
Size: 74 1815 124
Fragments arranged by size:
1815 124 74
BbvCI CC'TCA_GC
Cuts at: 0 957 1638 2013
Size: 957 681 375
Fragments arranged by size:
957 681 375
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1519 1553 2013
Size: 1519 34 460
Fragments arranged by size:
1519 460 34
BglI GCCn_nnn'nGGC
Cuts at: 0 952 1277 2013
Size: 952 325 736
Fragments arranged by size:
952 736 325
BlpI GC'TnA_GC
Cuts at: 0 34 844 1614 2013
Size: 34 810 770 399
Fragments arranged by size:
810 770 399 34
Bme1580I G_kGCm'C
Cuts at: 0 10 107 377 927 1495 2013
Size: 10 97 270 550 568 518
Fragments arranged by size:
568 550 518 270 97 10
BmgBI CAC'GTC
Cuts at: 0 1586 2013
Size: 1586 427
BmrI ACTGGGnnnn_n'
Cuts at: 0 761 1377 2013
Size: 761 616 636
Fragments arranged by size:
761 636 616
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 816 2013
Size: 816 1197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 659 844 954 984 1273 1381 1426
Size: 49 610 185 110 30 289 108 45
Cuts at: 1426 1912 2013
Size: 486 101
Fragments arranged by size:
610 486 289 185 110 108 101 49
45 30
Bpu10I CC'TnA_GC
Cuts at: 0 957 1328 1638 2013
Size: 957 371 310 375
Fragments arranged by size:
957 375 371 310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 2013
Size: 1144 869
BsaI GGTCTCn'nnnn_
Cuts at: 0 509 874 1082 2013
Size: 509 365 208 931
Fragments arranged by size:
931 509 365 208
BsaAI yAC'GTr
Cuts at: 0 317 1527 2013
Size: 317 1210 486
Fragments arranged by size:
1210 486 317
BsaHI Gr'CG_yC
Cuts at: 0 684 913 1500 2013
Size: 684 229 587 513
Fragments arranged by size:
684 587 513 229
BsaWI w'CCGG_w
Cuts at: 0 113 440 464 2013
Size: 113 327 24 1549
Fragments arranged by size:
1549 327 113 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1361 1391 1535 1565 1615 1645 1800 1830
Size: 1361 30 144 30 50 30 155 30
Cuts at: 1830 2013
Size: 183
Fragments arranged by size:
1361 183 155 144 50 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 517 1803 2013
Size: 517 1286 210
Fragments arranged by size:
1286 517 210
BseYI C'CCAG_C
Cuts at: 0 28 209 290 350 536 590 803 1119
Size: 28 181 81 60 186 54 213 316
Cuts at: 1119 1574 1627 1653 1786 2013
Size: 455 53 26 133 227
Fragments arranged by size:
455 316 227 213 186 181 133 81
60 54 53 28 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1549 2013
Size: 1549 464
BsiEI CG_ry'CG
Cuts at: 0 947 1272 2013
Size: 947 325 741
Fragments arranged by size:
947 741 325
BsiHKAI G_wGCw'C
Cuts at: 0 680 907 970 1265 1439 2013
Size: 680 227 63 295 174 574
Fragments arranged by size:
680 574 295 227 174 63
BsmI GAATG_Cn'
Cuts at: 0 643 2013
Size: 643 1370
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1592 2013
Size: 1592 421
Bsp1286I G_dGCh'C
Cuts at: 0 10 35 107 377 537 680 907 927
Size: 10 25 72 270 160 143 227 20
Cuts at: 927 970 1265 1439 1495 2013
Size: 43 295 174 56 518
Fragments arranged by size:
518 295 270 227 174 160 143 72
56 43 25 20 10
BspEI T'CCGG_A
Cuts at: 0 113 2013
Size: 113 1900
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 555 875 1521 2013
Size: 555 320 646 492
Fragments arranged by size:
646 555 492 320
BsrBI CCG'CTC
Cuts at: 0 389 1613 2013
Size: 389 1224 400
Fragments arranged by size:
1224 400 389
BsrDI GCAATG_nn'
Cuts at: 0 183 398 2013
Size: 183 215 1615
Fragments arranged by size:
1615 215 183
BsrFI r'CCGG_y
Cuts at: 0 909 946 2013
Size: 909 37 1067
Fragments arranged by size:
1067 909 37
BssSI C'ACGA_G
Cuts at: 0 1538 2013
Size: 1538 475
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1671 1747 2013
Size: 1671 76 266
Fragments arranged by size:
1671 266 76
BstYI r'GATC_y
Cuts at: 0 278 1322 2013
Size: 278 1044 691
Fragments arranged by size:
1044 691 278
BtgI C'CryG_G
Cuts at: 0 377 1266 2013
Size: 377 889 747
Fragments arranged by size:
889 747 377
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1870 2013
Size: 1870 143
BtsI GCAGTG_nn'
Cuts at: 0 1936 2013
Size: 1936 77
DrdI GACnn_nn'nnGTC
Cuts at: 0 717 2013
Size: 717 1296
EaeI y'GGCC_r
Cuts at: 0 57 241 836 944 1269 2013
Size: 57 184 595 108 325 744
Fragments arranged by size:
744 595 325 184 108 57
EagI C'GGCC_G
Cuts at: 0 944 1269 2013
Size: 944 325 744
Fragments arranged by size:
944 744 325
EarI CTCTTCn'nnn_
Cuts at: 0 599 710 1127 1135 2013
Size: 599 111 417 8 878
Fragments arranged by size:
878 599 417 111 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 990 2013
Size: 990 1023
EcoICRI GAG'CTC
Cuts at: 0 905 968 2013
Size: 905 63 1045
Fragments arranged by size:
1045 905 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 623 659 844 954 984 1273 1381
Size: 49 574 36 185 110 30 289 108
Cuts at: 1381 1426 1912 2013
Size: 45 486 101
Fragments arranged by size:
574 486 289 185 110 108 101 49
45 36 30
EcoO109I rG'GnC_Cy
Cuts at: 0 6 247 864 924 1401 1783 1808 1871
Size: 6 241 617 60 477 382 25 63
Cuts at: 1871 1967 2013
Size: 96 46
Fragments arranged by size:
617 477 382 241 96 63 60 46
25 6
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1185 2013
Size: 1185 828
FseI GG_CCGG'CC
Cuts at: 0 950 2013
Size: 950 1063
FspI TGC'GCA
Cuts at: 0 1648 2013
Size: 1648 365
HaeII r_GCGC'y
Cuts at: 0 351 851 916 1503 2013
Size: 351 500 65 587 510
Fragments arranged by size:
587 510 500 351 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1424 1456 1728 1760 1800 1832 2013
Size: 1424 32 272 32 40 32 181
Fragments arranged by size:
1424 272 181 40 32 32 32
HincII GTy'rAC
Cuts at: 0 420 2013
Size: 420 1593
KasI G'GCGC_C
Cuts at: 0 912 1499 2013
Size: 912 587 514
Fragments arranged by size:
912 587 514
KpnI G_GTAC'C
Cuts at: 0 441 2013
Size: 441 1572
MfeI C'AATT_G
Cuts at: 0 875 2013
Size: 875 1138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 1036 2013
Size: 152 884 977
Fragments arranged by size:
977 884 152
MscI TGG'CCA
Cuts at: 0 59 243 2013
Size: 59 184 1770
Fragments arranged by size:
1770 184 59
MslI CAynn'nnrTG
Cuts at: 0 814 2013
Size: 814 1199
MspA1I CmG'CkG
Cuts at: 0 486 774 936 1603 1657 2013
Size: 486 288 162 667 54 356
Fragments arranged by size:
667 486 356 288 162 54
NaeI GCC'GGC
Cuts at: 0 911 948 2013
Size: 911 37 1065
Fragments arranged by size:
1065 911 37
NarI GG'CG_CC
Cuts at: 0 913 1500 2013
Size: 913 587 513
Fragments arranged by size:
913 587 513
NgoMIV G'CCGG_C
Cuts at: 0 909 946 2013
Size: 909 37 1067
Fragments arranged by size:
1067 909 37
NspI r_CATG'y
Cuts at: 0 396 985 2013
Size: 396 589 1028
Fragments arranged by size:
1028 589 396
PasI CC'CwG_GG
Cuts at: 0 250 2013
Size: 250 1763
PflMI CCAn_nnn'nTGG
Cuts at: 0 250 434 1026 1359 1797 2013
Size: 250 184 592 333 438 216
Fragments arranged by size:
592 438 333 250 216 184
PfoI T'CCnGG_A
Cuts at: 0 721 1960 2013
Size: 721 1239 53
Fragments arranged by size:
1239 721 53
PmlI CAC'GTG
Cuts at: 0 317 2013
Size: 317 1696
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1535 1567 2013
Size: 1535 32 446
Fragments arranged by size:
1535 446 32
PpuMI rG'GwC_Cy
Cuts at: 0 864 1401 1783 1808 1967 2013
Size: 864 537 382 25 159 46
Fragments arranged by size:
864 537 382 159 46 25
PshAI GACnn'nnGTC
Cuts at: 0 891 2013
Size: 891 1122
PspOMI G'GGCC_C
Cuts at: 0 6 373 923 2013
Size: 6 367 550 1090
Fragments arranged by size:
1090 550 367 6
PstI C_TGCA'G
Cuts at: 0 662 740 1205 2013
Size: 662 78 465 808
Fragments arranged by size:
808 662 465 78
PvuII CAG'CTG
Cuts at: 0 486 1657 2013
Size: 486 1171 356
Fragments arranged by size:
1171 486 356
SacI G_AGCT'C
Cuts at: 0 907 970 2013
Size: 907 63 1043
Fragments arranged by size:
1043 907 63
SexAI A'CCwGG_T
Cuts at: 0 1352 2013
Size: 1352 661
SfcI C'TryA_G
Cuts at: 0 630 658 736 1201 2013
Size: 630 28 78 465 812
Fragments arranged by size:
812 630 465 78 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 952 2013
Size: 952 1061
SfoI GGC'GCC
Cuts at: 0 914 1501 2013
Size: 914 587 512
Fragments arranged by size:
914 587 512
SgrAI Cr'CCGG_yG
Cuts at: 0 909 2013
Size: 909 1104
SmaI CCC'GGG
Cuts at: 0 91 2013
Size: 91 1922
SmlI C'TyrA_G
Cuts at: 0 1159 1778 2013
Size: 1159 619 235
Fragments arranged by size:
1159 619 235
SphI G_CATG'C
Cuts at: 0 396 2013
Size: 396 1617
StyI C'CwwG_G
Cuts at: 0 323 1050 1187 1503 2013
Size: 323 727 137 316 510
Fragments arranged by size:
727 510 323 316 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 313 525 1732 1942 2013
Size: 313 212 1207 210 71
Fragments arranged by size:
1207 313 212 210 71
TatI w'GTAC_w
Cuts at: 0 1385 1865 2013
Size: 1385 480 148
Fragments arranged by size:
1385 480 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 52 84 211 243 1361 1393 1615 1647
Size: 52 32 127 32 1118 32 222 32
Cuts at: 1647 1743 1775 2013
Size: 96 32 238
Fragments arranged by size:
1118 238 222 127 96 52 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 703 1066 1216 2013
Size: 703 363 150 797
Fragments arranged by size:
797 703 363 150
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 771 1794 2013
Size: 771 1023 219
Fragments arranged by size:
1023 771 219
XhoI C'TCGA_G
Cuts at: 0 1778 2013
Size: 1778 235
XmaI C'CCGG_G
Cuts at: 0 89 2013
Size: 89 1924
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AlfI AloI
AlwNI ApaI ApoI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstXI
BstYI BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MfeI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AflIII AgeI AhdI AleI ApaLI AscI
AseI AsiSI BaeI BamHI BciVI BclI BfrBI BglII
BmtI BsaBI BsiWI BspHI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoNI
EcoRI EcoRV FspAI HindIII HpaI MluI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SgfI SnaBI SpeI SrfI SspI StuI
SwaI XbaI XmnI ZraI