(Circular) (Six-base) MAPSORT of: pFN21AB6223.seq Check: 9746 from: 1 to: 6715 pFN21AB6223 6715 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2989 4525 278 Size: 53 83 186 936 1453 1536 2468 Fragments arranged by size: 2468 1536 1453 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4058 1937 Size: 2121 4594 Acc65I G'GTAC_C Cuts at: 4036 4036 Size: 6715 AclI AA'CG_TT Cuts at: 1563 4842 5215 1563 Size: 3279 373 3063 Fragments arranged by size: 3279 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2416 2763 4023 4778 6162 6430 1632 Size: 784 347 1260 755 1384 268 1917 Fragments arranged by size: 1917 1384 1260 784 755 347 268 AfeI AGC'GCT Cuts at: 3632 3632 Size: 6715 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6518 AflIII A'CryG_T Cuts at: 1203 2194 5614 6591 1203 Size: 991 3420 977 1327 Fragments arranged by size: 3420 1327 991 977 AgeI A'CCGG_T Cuts at: 5546 5546 Size: 6715 AhdI GACnn_n'nnGTC Cuts at: 5444 5444 Size: 6715 AleI CACnn'nnGTG Cuts at: 1166 2313 3414 1166 Size: 1147 1101 4467 Fragments arranged by size: 4467 1147 1101 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6715 AlwNI CAG_nnn'CTG Cuts at: 4458 6030 4458 Size: 1572 5143 ApaLI G'TGCA_C Cuts at: 4772 5928 4772 Size: 1156 5559 ApoI r'AATT_y Cuts at: 1493 2817 2966 3321 3464 3573 4024 4250 4402 Size: 1324 149 355 143 109 451 226 152 Cuts at: 4402 6511 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1324 451 355 226 152 149 143 109 AseI AT'TA_AT Cuts at: 160 1032 5269 160 Size: 872 4237 1606 Fragments arranged by size: 4237 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6715 AvaI C'yCGr_G Cuts at: 1870 1943 4040 1870 Size: 73 2097 4545 Fragments arranged by size: 4545 2097 73 BamHI G'GATC_C Cuts at: 4045 4412 4045 Size: 367 6348 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4036 5491 618 Size: 325 217 627 2249 1455 1842 Fragments arranged by size: 2249 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2051 2139 2481 3803 4034 1391 Size: 660 88 342 1322 231 4072 Fragments arranged by size: 4072 1322 660 342 231 88 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5744 BbvCI CC'TCA_GC Cuts at: 2069 2069 Size: 6715 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4906 4940 1903 Size: 34 50 34 2885 34 3678 Fragments arranged by size: 3678 2885 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4607 5817 4607 Size: 1210 5505 BclI T'GATC_A Cuts at: 1548 1548 Size: 6715 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5326 136 Size: 107 122 71 631 605 3654 1525 Fragments arranged by size: 3654 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6710 6710 Size: 6715 BlpI GC'TnA_GC Cuts at: 2364 4132 2364 Size: 1768 4947 Bme1580I G_kGCm'C Cuts at: 1790 4776 5932 1790 Size: 2986 1156 2573 Fragments arranged by size: 2986 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6715 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2330 2598 4446 Size: 416 53 83 399 24 913 268 1848 Cuts at: 4446 5404 442 Size: 958 1753 Fragments arranged by size: 1848 1753 958 913 416 399 268 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3723 1056 Size: 2667 4048 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 2931 3015 3099 3183 3267 3519 3687 Size: 962 730 84 84 84 84 252 168 Cuts at: 3687 3771 3855 3939 5375 1239 Size: 84 84 84 1436 2579 Fragments arranged by size: 2579 1436 962 730 252 168 84 84 84 84 84 84 84 Bpu10I CC'TnA_GC Cuts at: 2069 2082 2069 Size: 13 6702 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4840 5705 6003 4840 Size: 865 298 5552 Fragments arranged by size: 5552 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5378 882 Size: 617 3879 2219 Fragments arranged by size: 3879 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4506 493 Size: 711 3302 2702 Fragments arranged by size: 3302 2702 711 BsaBI GATnn'nnATC Cuts at: 6709 6709 Size: 6715 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2986 4522 4904 5539 Size: 53 83 186 936 1453 1536 382 635 Cuts at: 5539 275 Size: 1451 Fragments arranged by size: 1536 1453 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3567 3651 5148 5546 5820 5967 6378 3567 Size: 84 1497 398 274 147 411 3904 Fragments arranged by size: 3904 1497 411 398 274 147 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6490 Fragments arranged by size: 6490 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2627 3012 1186 Size: 1441 385 4889 Fragments arranged by size: 4889 1441 385 BseYI C'CCAG_C Cuts at: 2566 5918 6414 2566 Size: 3352 496 2867 Fragments arranged by size: 3352 2867 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3286 3454 3622 3874 1163 Size: 573 1550 168 168 252 4004 Fragments arranged by size: 4004 1550 573 252 168 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 2023 4165 4926 5954 1540 Size: 275 186 18 4 2142 761 1028 2301 Fragments arranged by size: 2301 2142 1028 761 275 186 18 4 BsiHKAI G_wGCw'C Cuts at: 2051 2139 2370 3803 4776 4861 5932 2051 Size: 88 231 1433 973 85 1071 2834 Fragments arranged by size: 2834 1433 1071 973 231 88 85 BsmI GAATG_Cn' Cuts at: 2933 3353 3405 3521 3941 4226 4319 2933 Size: 420 52 116 420 285 93 5329 Fragments arranged by size: 5329 420 420 285 116 93 52 BsmBI CGTCTCn'nnnn_ Cuts at: 2977 2977 Size: 6715 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2139 2370 2481 3803 4034 4776 Size: 399 261 88 231 111 1322 231 742 Cuts at: 4776 4861 5932 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1322 1071 742 399 261 231 231 111 88 85 BspEI T'CCGG_A Cuts at: 6378 6378 Size: 6715 BspHI T'CATG_A Cuts at: 3007 4604 6334 3007 Size: 1597 1730 3388 Fragments arranged by size: 3388 1730 1597 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4070 844 Size: 3226 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2028 4602 1394 Size: 429 205 2574 3507 Fragments arranged by size: 3507 2574 429 205 BsrDI GCAATG_nn' Cuts at: 65 1248 5210 5384 65 Size: 1183 3962 174 1396 Fragments arranged by size: 3962 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2516 5359 5546 1700 Size: 110 706 2843 187 2869 Fragments arranged by size: 2869 2843 706 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6715 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6715 BssSI C'ACGA_G Cuts at: 4775 5787 4775 Size: 1012 5703 BstAPI GCAn_nnn'nTGC Cuts at: 794 2877 794 Size: 2083 4632 BstBI TT'CG_AA Cuts at: 5519 5519 Size: 6715 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6715 BstYI r'GATC_y Cuts at: 1972 4045 4412 4797 4814 6266 6352 6364 6710 Size: 2073 367 385 17 1452 86 12 346 Cuts at: 6710 1972 Size: 1977 Fragments arranged by size: 2073 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2042 2218 2574 513 Size: 552 977 176 356 4654 Fragments arranged by size: 4654 977 552 356 176 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2184 487 Size: 683 56 958 5018 Fragments arranged by size: 5018 958 683 56 BtsI GCAGTG_nn' Cuts at: 2938 3274 3442 3526 3610 3946 4231 5025 5045 Size: 336 168 84 84 336 285 794 20 Cuts at: 5045 2938 Size: 4608 Fragments arranged by size: 4608 794 336 336 285 168 84 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6715 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2111 2146 529 Size: 35 1547 35 5098 Fragments arranged by size: 5098 1547 35 35 DraI TTT'AAA Cuts at: 4019 4019 Size: 6715 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5722 809 Size: 507 4406 1802 Fragments arranged by size: 4406 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2020 2106 4162 Size: 54 1425 50 275 18 190 86 2056 Cuts at: 4162 5051 6680 8 Size: 889 1629 43 Fragments arranged by size: 2056 1629 1425 889 275 190 86 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2020 4162 1537 Size: 275 208 2142 4090 Fragments arranged by size: 4090 2142 275 208 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 2469 3229 4645 1353 Size: 768 348 760 1416 3423 Fragments arranged by size: 3423 1416 768 760 348 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5302 5676 5822 387 Size: 1064 138 3713 374 146 1280 Fragments arranged by size: 3713 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2049 2137 3801 2049 Size: 88 1664 4963 Fragments arranged by size: 4963 1664 88 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2416 2763 2931 3015 3099 3183 Size: 393 569 215 347 168 84 84 84 Cuts at: 3183 3267 3519 3687 3771 3855 3939 4023 4778 Size: 84 252 168 84 84 84 84 755 Cuts at: 4778 5375 6162 6430 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 755 597 569 393 347 268 252 215 168 168 84 84 84 84 84 84 84 84 EcoNI CCTnn'n_nnAGG Cuts at: 2485 2485 Size: 6715 EcoRI G'AATT_C Cuts at: 2966 3321 3573 4024 4402 2966 Size: 355 252 451 378 5279 Fragments arranged by size: 5279 451 378 355 252 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2416 2768 2416 Size: 352 6363 FspI TGC'GCA Cuts at: 5221 5221 Size: 6715 HaeII r_GCGC'y Cuts at: 1678 3634 5862 1678 Size: 1956 2228 2531 Fragments arranged by size: 2531 2228 1956 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5356 5388 5430 5462 1373 Size: 32 135 32 3784 32 42 32 2626 Fragments arranged by size: 3784 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4059 4305 669 Size: 988 281 2121 246 3079 Fragments arranged by size: 3079 2121 988 281 246 HindIII A'AGCT_T Cuts at: 748 2694 748 Size: 1946 4769 HpaI GTT'AAC Cuts at: 4305 4305 Size: 6715 KpnI G_GTAC'C Cuts at: 4040 4040 Size: 6715 MfeI C'AATT_G Cuts at: 4314 4314 Size: 6715 MluI A'CGCG_T Cuts at: 6591 6591 Size: 6715 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2611 5829 6013 1305 Size: 1306 3218 184 2007 Fragments arranged by size: 3218 2007 1306 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2108 10 Size: 54 1425 343 276 4617 Fragments arranged by size: 4617 1425 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2313 2414 2466 2730 3012 3414 4673 Size: 648 1147 101 52 264 282 402 1259 Cuts at: 4673 5032 5191 518 Size: 359 159 2042 Fragments arranged by size: 2042 1259 1147 648 402 359 282 264 159 101 52 MspA1I CmG'CkG Cuts at: 1606 4131 4808 5956 6201 1606 Size: 2525 677 1148 245 2120 Fragments arranged by size: 2525 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2218 513 Size: 552 1153 5010 Fragments arranged by size: 5010 1153 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6715 NheI G'CTAG_C Cuts at: 1052 3719 1052 Size: 2667 4048 NotI GC'GGCC_GC Cuts at: 4162 4162 Size: 6715 NspI r_CATG'y Cuts at: 1132 1229 2198 4073 5618 1132 Size: 97 969 1875 1545 2229 Fragments arranged by size: 2229 1875 1545 969 97 PasI CC'CwG_GG Cuts at: 1348 2206 1348 Size: 858 5857 PciI A'CATG_T Cuts at: 2194 5614 2194 Size: 3420 3295 PflMI CCAn_nnn'nTGG Cuts at: 1108 2224 1108 Size: 1116 5599 PmeI GTTT'AAAC Cuts at: 4019 4019 Size: 6715 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2722 2754 4784 4816 6297 6329 2722 Size: 32 2030 32 1481 32 3108 Fragments arranged by size: 3108 2030 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6715 PsiI TTA'TAA Cuts at: 4285 4285 Size: 6715 PstI C_TGCA'G Cuts at: 830 2525 4067 830 Size: 1695 1542 3478 Fragments arranged by size: 3478 1695 1542 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6715 SacI G_AGCT'C Cuts at: 2051 2139 3803 2051 Size: 88 1664 4963 Fragments arranged by size: 4963 1664 88 SalI G'TCGA_C Cuts at: 1936 4057 1936 Size: 2121 4594 SapI GCTCTTCn'nnn_ Cuts at: 2469 2469 Size: 6715 SbfI CC_TGCA'GG Cuts at: 4067 4067 Size: 6715 ScaI AGT'ACT Cuts at: 4963 6675 4963 Size: 1712 5003 SfcI C'TryA_G Cuts at: 826 1045 2521 4063 5198 5879 6070 826 Size: 219 1476 1542 1135 681 191 1471 Fragments arranged by size: 1542 1476 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6715 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6715 SmaI CCC'GGG Cuts at: 1872 4042 1872 Size: 2170 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4819 5720 5982 820 Size: 197 926 2876 901 262 1553 Fragments arranged by size: 2876 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4506 493 Size: 4013 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6715 SphI G_CATG'C Cuts at: 1132 4073 1132 Size: 2941 3774 SspI AAT'ATT Cuts at: 5 52 4639 5 Size: 47 4587 2081 Fragments arranged by size: 4587 2081 47 StuI AGG'CCT Cuts at: 1517 2561 2920 3088 3172 3424 3676 3760 3928 Size: 1044 359 168 84 252 252 84 168 Cuts at: 3928 4012 1517 Size: 84 4220 Fragments arranged by size: 4220 1044 359 252 252 168 168 84 84 84 StyI C'CwwG_G Cuts at: 513 1065 2218 4154 513 Size: 552 1153 1936 3074 Fragments arranged by size: 3074 1936 1153 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2308 2312 4742 4759 4912 5097 5542 Size: 1255 728 4 2430 17 153 185 445 Cuts at: 5542 325 Size: 1498 Fragments arranged by size: 2430 1498 1255 728 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2163 4961 6673 Size: 274 80 33 51 1443 186 2798 1712 Cuts at: 6673 96 Size: 138 Fragments arranged by size: 2798 1712 1443 274 186 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6675 6707 6675 Size: 32 6683 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6715 XbaI T'CTAG_A Cuts at: 4051 4051 Size: 6715 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6715 XmaI C'CCGG_G Cuts at: 1870 4040 1870 Size: 2170 4545 XmnI GAAnn'nnTTC Cuts at: 2543 2695 2780 2970 3306 4844 2543 Size: 152 85 190 336 1538 4414 Fragments arranged by size: 4414 1538 336 190 152 85 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2987 4523 276 Size: 53 83 186 936 1453 1536 2468 Fragments arranged by size: 2468 1536 1453 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI