(Circular) (Six-base) MAPSORT of: pFN21AB6217.seq Check: 3580 from: 1 to: 6889 pFN21AB6217 6889 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3412 3412 Size: 6889 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4699 278 Size: 53 83 186 936 3163 2468 Fragments arranged by size: 3163 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4232 1937 Size: 2295 4594 Acc65I G'GTAC_C Cuts at: 2598 2934 4210 2598 Size: 336 1276 5277 Fragments arranged by size: 5277 1276 336 AclI AA'CG_TT Cuts at: 1563 5016 5389 1563 Size: 3453 373 3063 Fragments arranged by size: 3453 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2494 4952 6336 6604 1632 Size: 862 2458 1384 268 1917 Fragments arranged by size: 2458 1917 1384 862 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6692 AflIII A'CryG_T Cuts at: 1203 5788 6765 1203 Size: 4585 977 1327 Fragments arranged by size: 4585 1327 977 AgeI A'CCGG_T Cuts at: 5720 5720 Size: 6889 AhdI GACnn_n'nnGTC Cuts at: 5618 5618 Size: 6889 AleI CACnn'nnGTG Cuts at: 1166 2690 1166 Size: 1524 5365 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6889 AlwNI CAG_nnn'CTG Cuts at: 2272 2752 3221 3537 4073 4632 6204 2272 Size: 480 469 316 536 559 1572 2957 Fragments arranged by size: 2957 1572 559 536 480 469 316 ApaI G_GGCC'C Cuts at: 2069 2279 2782 3229 4127 4188 2069 Size: 210 503 447 898 61 4770 Fragments arranged by size: 4770 898 503 447 210 61 ApaLI G'TGCA_C Cuts at: 3239 4946 6102 3239 Size: 1707 1156 4026 Fragments arranged by size: 4026 1707 1156 ApoI r'AATT_y Cuts at: 1493 4198 4424 4576 6685 1493 Size: 2705 226 152 2109 1697 Fragments arranged by size: 2705 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5443 160 Size: 872 4411 1606 Fragments arranged by size: 4411 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6889 AvaI C'yCGr_G Cuts at: 1870 1943 2031 3228 4214 1870 Size: 73 88 1197 986 4545 Fragments arranged by size: 4545 1197 986 88 73 BamHI G'GATC_C Cuts at: 4219 4586 4219 Size: 367 6522 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2290 2390 2598 2934 4210 Size: 325 217 627 503 100 208 336 1276 Cuts at: 4210 5665 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1276 627 503 336 325 217 208 100 BanII G_rGCy'C Cuts at: 1391 2069 2076 2121 2241 2279 2355 2782 2791 Size: 678 7 45 120 38 76 427 9 Cuts at: 2791 3229 3897 4127 4148 4188 4208 1391 Size: 438 668 230 21 40 20 4072 Fragments arranged by size: 4072 678 668 438 427 230 120 76 45 40 38 21 20 9 7 BbeI G_GCGC'C Cuts at: 2294 2394 2294 Size: 100 6789 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2435 2551 3129 3679 928 Size: 971 536 116 578 550 4138 Fragments arranged by size: 4138 971 578 550 536 116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5080 5114 1903 Size: 34 3143 34 3678 Fragments arranged by size: 3678 3143 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4781 5991 4781 Size: 1210 5679 BclI T'GATC_A Cuts at: 1548 3456 1548 Size: 1908 4981 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2441 3975 5500 Size: 107 122 71 631 605 769 1534 1525 Cuts at: 5500 136 Size: 1525 Fragments arranged by size: 1534 1525 1525 769 631 605 122 107 71 BglII A'GATC_T Cuts at: 6884 6884 Size: 6889 BlpI GC'TnA_GC Cuts at: 2075 3891 4306 2075 Size: 1816 415 4658 Fragments arranged by size: 4658 1816 415 Bme1580I G_kGCm'C Cuts at: 1790 2060 2069 2279 2782 3229 3243 4127 4188 Size: 270 9 210 503 447 14 884 61 Cuts at: 4188 4950 6106 1790 Size: 762 1156 2573 Fragments arranged by size: 2573 1156 884 762 503 447 270 210 61 14 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6889 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3231 4620 5578 Size: 416 53 83 399 24 1814 1389 958 Cuts at: 5578 442 Size: 1753 Fragments arranged by size: 1814 1753 1389 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6889 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2227 3817 4015 2227 Size: 1590 198 5101 Fragments arranged by size: 5101 1590 198 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 2217 2219 2642 2788 3011 3545 3948 Size: 920 58 2 423 146 223 534 403 Cuts at: 3948 4068 4122 5549 1239 Size: 120 54 1427 2579 Fragments arranged by size: 2579 1427 920 534 423 403 223 146 120 58 54 2 Bpu10I CC'TnA_GC Cuts at: 3987 3987 Size: 6889 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5014 5879 6177 5014 Size: 865 298 5726 Fragments arranged by size: 5726 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2183 3046 3296 3966 5552 882 Size: 617 684 863 250 670 1586 2219 Fragments arranged by size: 2219 1586 863 684 670 617 250 BsaAI yAC'GTr Cuts at: 493 1204 4680 493 Size: 711 3476 2702 Fragments arranged by size: 3476 2702 711 BsaBI GATnn'nnATC Cuts at: 6883 6883 Size: 6889 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2291 2391 4696 5078 Size: 53 83 186 936 758 100 2305 382 Cuts at: 5078 5713 275 Size: 635 1451 Fragments arranged by size: 2305 1451 936 758 635 382 186 100 83 53 BsaWI w'CCGG_w Cuts at: 5322 5720 5994 6141 6552 5322 Size: 398 274 147 411 5659 Fragments arranged by size: 5659 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3026 3056 4084 4114 1178 Size: 30 165 30 1623 30 1028 30 3953 Fragments arranged by size: 3953 1623 1028 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2216 2276 2327 2330 2630 2639 3008 Size: 970 60 60 51 3 300 9 369 Cuts at: 3008 3565 3853 1186 Size: 557 288 4222 Fragments arranged by size: 4222 970 557 369 300 288 60 60 51 9 3 BseYI C'CCAG_C Cuts at: 2272 2295 2412 3004 4062 6092 6588 2272 Size: 23 117 592 1058 2030 496 2573 Fragments arranged by size: 2573 2030 1058 592 496 117 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2443 2563 3346 1163 Size: 573 707 120 783 4706 Fragments arranged by size: 4706 783 707 573 120 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4339 5100 6128 1540 Size: 275 186 2338 761 1028 2301 Fragments arranged by size: 2338 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 2355 2411 3243 3897 3988 4950 5035 6106 Size: 279 56 832 654 91 962 85 1071 Cuts at: 6106 2076 Size: 2859 Fragments arranged by size: 2859 1071 962 832 654 279 91 85 56 BsmI GAATG_Cn' Cuts at: 4400 4493 4400 Size: 93 6796 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2069 2076 2121 2241 2279 2355 Size: 399 270 9 7 45 120 38 76 Cuts at: 2355 2411 2782 2791 3229 3243 3897 3988 4127 Size: 56 371 9 438 14 654 91 139 Cuts at: 4127 4148 4188 4208 4950 5035 6106 1391 Size: 21 40 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 654 438 399 371 270 139 120 91 85 76 56 45 40 38 21 20 14 9 9 7 BspEI T'CCGG_A Cuts at: 6552 6552 Size: 6889 BspHI T'CATG_A Cuts at: 2121 2241 4778 6508 2121 Size: 120 2537 1730 2502 Fragments arranged by size: 2537 2502 1730 120 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2293 2727 3265 3412 3915 4244 844 Size: 1449 434 538 147 503 329 3489 Fragments arranged by size: 3489 1449 538 503 434 329 147 BsrBI CCG'CTC Cuts at: 1394 1823 2340 4776 1394 Size: 429 517 2436 3507 Fragments arranged by size: 3507 2436 517 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5384 5558 65 Size: 1183 4136 174 1396 Fragments arranged by size: 4136 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2696 5533 5720 1700 Size: 110 886 2837 187 2869 Fragments arranged by size: 2869 2837 886 187 110 BsrGI T'GTAC_A Cuts at: 96 2664 96 Size: 2568 4321 BssHII G'CGCG_C Cuts at: 1925 3178 1925 Size: 1253 5636 BssSI C'ACGA_G Cuts at: 2690 4949 5961 2690 Size: 2259 1012 3618 Fragments arranged by size: 3618 2259 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2743 794 Size: 1949 4940 BstBI TT'CG_AA Cuts at: 5693 5693 Size: 6889 BstEII G'GTnAC_C Cuts at: 1184 3814 1184 Size: 2630 4259 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2401 2692 2011 Size: 390 291 6208 Fragments arranged by size: 6208 390 291 BstYI r'GATC_y Cuts at: 1972 4219 4586 4971 4988 6440 6526 6538 6884 Size: 2247 367 385 17 1452 86 12 346 Cuts at: 6884 1972 Size: 1977 Fragments arranged by size: 2247 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2753 2753 Size: 6889 BtgI C'CryG_G Cuts at: 513 1065 2004 2304 2386 3491 513 Size: 552 939 300 82 1105 3911 Fragments arranged by size: 3911 1105 939 552 300 82 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6150 Fragments arranged by size: 6150 683 56 BtsI GCAGTG_nn' Cuts at: 2451 2574 3382 4405 5199 5219 2451 Size: 123 808 1023 794 20 4121 Fragments arranged by size: 4121 1023 808 794 123 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6889 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3448 3483 529 Size: 35 2884 35 3935 Fragments arranged by size: 3935 2884 35 35 DraI TTT'AAA Cuts at: 4193 4193 Size: 6889 DraIII CAC_nnn'GTG Cuts at: 3603 3603 Size: 6889 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2555 3911 5896 809 Size: 507 1239 1356 1985 1802 Fragments arranged by size: 1985 1802 1356 1239 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2694 3744 Size: 54 1425 50 275 18 183 681 1050 Cuts at: 3744 4336 5225 6854 8 Size: 592 889 1629 43 Fragments arranged by size: 1629 1425 1050 889 681 592 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4336 1537 Size: 275 2524 4090 Fragments arranged by size: 4090 2524 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2360 4819 1353 Size: 1007 2459 3423 Fragments arranged by size: 3423 2459 1007 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2324 2387 4156 5476 5850 5996 Size: 1064 138 735 63 1769 1320 374 146 Cuts at: 5996 387 Size: 1280 Fragments arranged by size: 1769 1320 1280 1064 735 374 146 138 63 EcoICRI GAG'CTC Cuts at: 2074 2353 3895 2074 Size: 279 1542 5068 Fragments arranged by size: 5068 1542 279 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2217 2219 2494 2642 2788 3011 Size: 393 527 58 2 275 148 146 223 Cuts at: 3011 3545 3948 4068 4122 4952 5549 6336 6604 Size: 534 403 120 54 830 597 787 268 Cuts at: 6604 1239 Size: 1524 Fragments arranged by size: 1524 830 787 597 534 527 403 393 275 268 223 148 146 120 58 54 2 EcoNI CCTnn'n_nnAGG Cuts at: 2993 2993 Size: 6889 EcoO109I rG'GnC_Cy Cuts at: 2065 2149 2276 2320 2758 2778 3225 4070 4124 Size: 84 127 44 438 20 447 845 54 Cuts at: 4124 4184 2065 Size: 60 4770 Fragments arranged by size: 4770 845 447 438 127 84 60 54 44 20 EcoRI G'AATT_C Cuts at: 4198 4576 4198 Size: 378 6511 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2452 2575 2872 2452 Size: 123 297 6469 Fragments arranged by size: 6469 297 123 FspI TGC'GCA Cuts at: 5395 5395 Size: 6889 HaeII r_GCGC'y Cuts at: 1678 2294 2394 2752 6036 1678 Size: 616 100 358 3284 2531 Fragments arranged by size: 3284 2531 616 358 100 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 3560 3592 4084 Size: 32 135 32 509 32 1447 32 492 Cuts at: 4084 4116 5530 5562 5604 5636 1373 Size: 32 1414 32 42 32 2626 Fragments arranged by size: 2626 1447 1414 509 492 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3568 3724 3754 4233 4479 669 Size: 988 281 1630 156 30 479 246 3079 Fragments arranged by size: 3079 1630 988 479 281 246 156 30 HindIII A'AGCT_T Cuts at: 748 748 Size: 6889 HpaI GTT'AAC Cuts at: 4479 4479 Size: 6889 KasI G'GCGC_C Cuts at: 2290 2390 2290 Size: 100 6789 KpnI G_GTAC'C Cuts at: 2602 2938 4214 2602 Size: 336 1276 5277 Fragments arranged by size: 5277 1276 336 MfeI C'AATT_G Cuts at: 4488 4488 Size: 6889 MluI A'CGCG_T Cuts at: 6765 6765 Size: 6889 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3147 3852 6003 6187 1305 Size: 1842 705 2151 184 2007 Fragments arranged by size: 2151 2007 1842 705 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2015 3746 10 Size: 54 1425 343 183 1731 3153 Fragments arranged by size: 3153 1731 1425 343 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2690 3314 4847 5206 5365 518 Size: 648 843 681 624 1533 359 159 2042 Fragments arranged by size: 2042 1533 843 681 648 624 359 159 MspA1I CmG'CkG Cuts at: 1606 2863 3493 4305 4982 6130 6375 1606 Size: 1257 630 812 677 1148 245 2120 Fragments arranged by size: 2120 1257 1148 812 677 630 245 NaeI GCC'GGC Cuts at: 2698 2698 Size: 6889 NarI GG'CG_CC Cuts at: 2291 2391 2291 Size: 100 6789 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5398 Fragments arranged by size: 5398 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6889 NgoMIV G'CCGG_C Cuts at: 2696 2696 Size: 6889 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6889 NotI GC'GGCC_GC Cuts at: 4336 4336 Size: 6889 NruI TCG'CGA Cuts at: 2715 3703 2715 Size: 988 5901 NspI r_CATG'y Cuts at: 1132 1229 4247 5792 1132 Size: 97 3018 1545 2229 Fragments arranged by size: 3018 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4120 1348 Size: 2772 4117 PciI A'CATG_T Cuts at: 5788 5788 Size: 6889 PflMI CCAn_nnn'nTGG Cuts at: 1108 3129 3505 1108 Size: 2021 376 4492 Fragments arranged by size: 4492 2021 376 PfoI T'CCnGG_A Cuts at: 3444 3444 Size: 6889 PmeI GTTT'AAAC Cuts at: 4193 4193 Size: 6889 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4958 4990 6471 6503 4958 Size: 32 1481 32 5344 Fragments arranged by size: 5344 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2149 2758 2149 Size: 609 6280 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6889 PsiI TTA'TAA Cuts at: 4459 4459 Size: 6889 PspOMI G'GGCC_C Cuts at: 2065 2275 2778 3225 4123 4184 2065 Size: 210 503 447 898 61 4770 Fragments arranged by size: 4770 898 503 447 210 61 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3494 3526 3494 Size: 32 6857 PstI C_TGCA'G Cuts at: 830 2166 4153 4241 830 Size: 1336 1987 88 3478 Fragments arranged by size: 3478 1987 1336 88 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6889 PvuII CAG'CTG Cuts at: 2863 2863 Size: 6889 SacI G_AGCT'C Cuts at: 2076 2355 3897 2076 Size: 279 1542 5068 Fragments arranged by size: 5068 1542 279 SacII CC_GC'GG Cuts at: 3494 3494 Size: 6889 SalI G'TCGA_C Cuts at: 1936 4231 1936 Size: 2295 4594 SanDI GG'GwC_CC Cuts at: 2758 2758 Size: 6889 SapI GCTCTTCn'nnn_ Cuts at: 2360 2360 Size: 6889 SbfI CC_TGCA'GG Cuts at: 4241 4241 Size: 6889 ScaI AGT'ACT Cuts at: 2507 3512 5137 6849 2507 Size: 1005 1625 1712 2547 Fragments arranged by size: 2547 1712 1625 1005 SexAI A'CCwGG_T Cuts at: 2173 3036 2173 Size: 863 6026 SfcI C'TryA_G Cuts at: 826 1045 2162 2593 3520 4149 4237 5372 6053 Size: 219 1117 431 927 629 88 1135 681 Cuts at: 6053 6244 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1117 927 681 629 431 219 191 88 SfoI GGC'GCC Cuts at: 2292 2392 2292 Size: 100 6789 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6889 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6889 SmaI CCC'GGG Cuts at: 1872 3230 4216 1872 Size: 1358 986 4545 Fragments arranged by size: 4545 1358 986 SmlI C'TyrA_G Cuts at: 820 1017 1943 4993 5894 6156 820 Size: 197 926 3050 901 262 1553 Fragments arranged by size: 3050 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4680 493 Size: 4187 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6889 SphI G_CATG'C Cuts at: 1132 4247 1132 Size: 3115 3774 SspI AAT'ATT Cuts at: 5 52 4813 5 Size: 47 4761 2081 Fragments arranged by size: 4761 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6889 StyI C'CwwG_G Cuts at: 513 1065 2004 4328 513 Size: 552 939 2324 3074 Fragments arranged by size: 3074 2324 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4916 4933 5086 5271 5716 325 Size: 1255 3336 17 153 185 445 1498 Fragments arranged by size: 3336 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2475 2505 2664 Size: 274 80 33 51 1443 498 30 159 Cuts at: 2664 3411 3510 5135 6847 96 Size: 747 99 1625 1712 138 Fragments arranged by size: 1712 1625 1443 747 498 274 159 138 99 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2020 2052 2242 2274 2572 2604 6849 6881 2020 Size: 32 190 32 298 32 4245 32 2028 Fragments arranged by size: 4245 2028 298 190 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6889 XbaI T'CTAG_A Cuts at: 4225 4225 Size: 6889 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3083 3641 3083 Size: 558 6331 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6889 XmaI C'CCGG_G Cuts at: 1870 3228 4214 1870 Size: 1358 986 4545 Fragments arranged by size: 4545 1358 986 XmnI GAAnn'nnTTC Cuts at: 3194 5018 3194 Size: 1824 5065 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4697 276 Size: 53 83 186 936 3163 2468 Fragments arranged by size: 3163 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NsiI PacI PmlI PspXI RsrII SfiI SrfI SwaI