(Circular) (Six-base) MAPSORT of: pFN21AB6190.seq Check: 6785 from: 1 to: 6682 pFN21AB6190 6682 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4492 278 Size: 53 83 186 936 2956 2468 Fragments arranged by size: 2956 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4025 1937 Size: 2088 4594 Acc65I G'GTAC_C Cuts at: 4003 4003 Size: 6682 AclI AA'CG_TT Cuts at: 1563 4809 5182 1563 Size: 3246 373 3063 Fragments arranged by size: 3246 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2422 2740 2935 3439 3523 4745 6129 6397 Size: 790 318 195 504 84 1222 1384 268 Cuts at: 6397 1632 Size: 1917 Fragments arranged by size: 1917 1384 1222 790 504 318 268 195 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6485 AflIII A'CryG_T Cuts at: 1203 2357 2487 3345 5581 6558 1203 Size: 1154 130 858 2236 977 1327 Fragments arranged by size: 2236 1327 1154 977 858 130 AgeI A'CCGG_T Cuts at: 5513 5513 Size: 6682 AhdI GACnn_n'nnGTC Cuts at: 5411 5411 Size: 6682 AleI CACnn'nnGTG Cuts at: 1166 2274 3473 1166 Size: 1108 1199 4375 Fragments arranged by size: 4375 1199 1108 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6682 AlwNI CAG_nnn'CTG Cuts at: 2350 4425 5997 2350 Size: 2075 1572 3035 Fragments arranged by size: 3035 2075 1572 ApaLI G'TGCA_C Cuts at: 2353 4739 5895 2353 Size: 2386 1156 3140 Fragments arranged by size: 3140 2386 1156 ApoI r'AATT_y Cuts at: 1493 2900 3069 3197 3237 3281 3533 3573 3785 Size: 1407 169 128 40 44 252 40 212 Cuts at: 3785 3991 4217 4369 6478 1493 Size: 206 226 152 2109 1697 Fragments arranged by size: 2109 1697 1407 252 226 212 206 169 152 128 44 40 40 AseI AT'TA_AT Cuts at: 160 1032 2805 5236 160 Size: 872 1773 2431 1606 Fragments arranged by size: 2431 1773 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6682 AvaI C'yCGr_G Cuts at: 1870 1943 2076 4007 1870 Size: 73 133 1931 4545 Fragments arranged by size: 4545 1931 133 73 BamHI G'GATC_C Cuts at: 4012 4379 4012 Size: 367 6315 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2191 4003 5458 618 Size: 325 217 627 404 1812 1455 1842 Fragments arranged by size: 1842 1812 1455 627 404 325 217 BanII G_rGCy'C Cuts at: 1391 3931 4001 1391 Size: 2540 70 4072 Fragments arranged by size: 4072 2540 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5711 BbvCI CC'TCA_GC Cuts at: 2742 2742 Size: 6682 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4873 4907 1903 Size: 34 2936 34 3678 Fragments arranged by size: 3678 2936 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4574 5784 4574 Size: 1210 5472 BclI T'GATC_A Cuts at: 1548 1548 Size: 6682 BfrBI ATG'CAT Cuts at: 2604 3267 2604 Size: 663 6019 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5293 136 Size: 107 122 71 631 605 3621 1525 Fragments arranged by size: 3621 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3002 6677 3002 Size: 3675 3007 BlpI GC'TnA_GC Cuts at: 4099 4099 Size: 6682 Bme1580I G_kGCm'C Cuts at: 1790 2357 4743 5899 1790 Size: 567 2386 1156 2573 Fragments arranged by size: 2573 2386 1156 567 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6682 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4413 5371 442 Size: 416 53 83 399 24 2996 958 1753 Fragments arranged by size: 2996 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6682 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2611 3015 3183 3253 3435 3519 3603 Size: 917 455 404 168 70 182 84 84 Cuts at: 3603 3619 3771 3855 3939 5342 1239 Size: 16 152 84 84 1403 2579 Fragments arranged by size: 2579 1403 917 455 404 182 168 152 84 84 84 84 70 16 Bpu10I CC'TnA_GC Cuts at: 2742 2742 Size: 6682 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2117 4807 5672 5970 2117 Size: 2690 865 298 2829 Fragments arranged by size: 2829 2690 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2743 5345 882 Size: 617 1244 2602 2219 Fragments arranged by size: 2602 2219 1244 617 BsaAI yAC'GTr Cuts at: 493 1204 2360 2508 3346 3850 4473 493 Size: 711 1156 148 838 504 623 2702 Fragments arranged by size: 2702 1156 838 711 623 504 148 BsaBI GATnn'nnATC Cuts at: 6676 6676 Size: 6682 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4489 4871 5506 275 Size: 53 83 186 936 2956 382 635 1451 Fragments arranged by size: 2956 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5115 5513 5787 5934 6345 5115 Size: 398 274 147 411 5452 Fragments arranged by size: 5452 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6457 Fragments arranged by size: 6457 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2114 1186 Size: 910 18 5754 Fragments arranged by size: 5754 910 18 BseYI C'CCAG_C Cuts at: 5885 6381 5885 Size: 496 6186 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2530 3202 3370 3454 3706 3790 3800 Size: 573 794 672 168 84 252 84 10 Cuts at: 3800 1163 Size: 4045 Fragments arranged by size: 4045 794 672 573 252 168 84 84 10 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4132 4893 5921 1540 Size: 275 186 2131 761 1028 2301 Fragments arranged by size: 2301 2131 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2357 3231 3931 4743 4828 5899 2357 Size: 874 700 812 85 1071 3140 Fragments arranged by size: 3140 1071 874 812 700 85 BsmI GAATG_Cn' Cuts at: 2606 3269 4193 4286 2606 Size: 663 924 93 5002 Fragments arranged by size: 5002 924 663 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2357 3231 3931 4001 4743 4828 5899 Size: 399 567 874 700 70 742 85 1071 Cuts at: 5899 1391 Size: 2174 Fragments arranged by size: 2174 1071 874 742 700 567 399 85 70 BspEI T'CCGG_A Cuts at: 6345 6345 Size: 6682 BspHI T'CATG_A Cuts at: 4571 6301 4571 Size: 1730 4952 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4037 844 Size: 3193 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4569 1394 Size: 429 2746 3507 Fragments arranged by size: 3507 2746 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3610 5177 5351 65 Size: 1183 2362 1567 174 1396 Fragments arranged by size: 2362 1567 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5326 5513 1700 Size: 110 3516 187 2869 Fragments arranged by size: 3516 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2477 3562 96 Size: 2381 1085 3216 Fragments arranged by size: 3216 2381 1085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6682 BssSI C'ACGA_G Cuts at: 3932 4742 5754 3932 Size: 810 1012 4860 Fragments arranged by size: 4860 1012 810 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6682 BstBI TT'CG_AA Cuts at: 5486 5486 Size: 6682 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6682 BstYI r'GATC_y Cuts at: 1972 2498 3002 3338 4012 4379 4764 4781 6233 Size: 526 504 336 674 367 385 17 1452 Cuts at: 6233 6319 6331 6677 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 674 526 504 385 367 346 336 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5191 Fragments arranged by size: 5191 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5943 Fragments arranged by size: 5943 683 56 BtsI GCAGTG_nn' Cuts at: 2938 3022 3190 3442 3694 3778 4198 4992 5012 Size: 84 168 252 252 84 420 794 20 Cuts at: 5012 2938 Size: 4608 Fragments arranged by size: 4608 794 420 252 252 168 84 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6682 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3088 3123 3592 3627 529 Size: 35 2524 35 469 35 3584 Fragments arranged by size: 3584 2524 469 35 35 35 DraI TTT'AAA Cuts at: 3986 3986 Size: 6682 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2245 5689 809 Size: 507 929 3444 1802 Fragments arranged by size: 3444 1802 929 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4129 5018 6647 Size: 54 1425 50 275 18 2299 889 1629 Cuts at: 6647 8 Size: 43 Fragments arranged by size: 2299 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4129 1537 Size: 275 2317 4090 Fragments arranged by size: 4090 2317 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2465 2721 4612 1353 Size: 1112 256 1891 3423 Fragments arranged by size: 3423 1891 1112 256 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5269 5643 5789 387 Size: 1064 138 3680 374 146 1280 Fragments arranged by size: 3680 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3929 3929 Size: 6682 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2422 2611 2740 2935 3015 3183 Size: 393 524 266 189 129 195 80 168 Cuts at: 3183 3253 3435 3439 3519 3523 3603 3619 3771 Size: 70 182 4 80 4 80 16 152 Cuts at: 3771 3855 3939 4745 5342 6129 6397 1239 Size: 84 84 806 597 787 268 1524 Fragments arranged by size: 1524 806 787 597 524 393 268 266 195 189 182 168 152 129 84 84 80 80 80 70 16 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 2099 2099 Size: 6682 EcoO109I rG'GnC_Cy Cuts at: 2457 3255 2457 Size: 798 5884 EcoRI G'AATT_C Cuts at: 3069 3237 3573 3991 4369 3069 Size: 168 336 418 378 5382 Fragments arranged by size: 5382 418 378 336 168 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3152 3404 3488 3152 Size: 252 84 6346 Fragments arranged by size: 6346 252 84 FspI TGC'GCA Cuts at: 5188 5188 Size: 6682 HaeII r_GCGC'y Cuts at: 1678 5829 1678 Size: 4151 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5323 5355 5397 5429 1373 Size: 32 135 32 3751 32 42 32 2626 Fragments arranged by size: 3751 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4026 4272 669 Size: 988 281 2088 246 3079 Fragments arranged by size: 3079 2088 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6682 HpaI GTT'AAC Cuts at: 4272 4272 Size: 6682 KpnI G_GTAC'C Cuts at: 4007 4007 Size: 6682 MfeI C'AATT_G Cuts at: 4281 4281 Size: 6682 MluI A'CGCG_T Cuts at: 6558 6558 Size: 6682 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3576 5796 5980 1305 Size: 2271 2220 184 2007 Fragments arranged by size: 2271 2220 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4860 Fragments arranged by size: 4860 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2272 2274 3473 4640 4999 5158 518 Size: 648 1106 2 1199 1167 359 159 2042 Fragments arranged by size: 2042 1199 1167 1106 648 359 159 2 MspA1I CmG'CkG Cuts at: 1606 2012 4098 4775 5923 6168 1606 Size: 406 2086 677 1148 245 2120 Fragments arranged by size: 2120 2086 1148 677 406 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5191 Fragments arranged by size: 5191 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6682 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6682 NotI GC'GGCC_GC Cuts at: 4129 4129 Size: 6682 NsiI A_TGCA'T Cuts at: 2606 3269 2606 Size: 663 6019 NspI r_CATG'y Cuts at: 1132 1229 2491 2931 4040 5585 1132 Size: 97 1262 440 1109 1545 2229 Fragments arranged by size: 2229 1545 1262 1109 440 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6682 PciI A'CATG_T Cuts at: 2487 5581 2487 Size: 3094 3588 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6682 PfoI T'CCnGG_A Cuts at: 2700 2700 Size: 6682 PmeI GTTT'AAAC Cuts at: 3986 3986 Size: 6682 PmlI CAC'GTG Cuts at: 2360 2360 Size: 6682 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2045 2077 4751 4783 6264 6296 2045 Size: 32 2674 32 1481 32 2431 Fragments arranged by size: 2674 2431 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6682 PsiI TTA'TAA Cuts at: 3093 4252 3093 Size: 1159 5523 PstI C_TGCA'G Cuts at: 830 3017 4034 830 Size: 2187 1017 3478 Fragments arranged by size: 3478 2187 1017 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6682 SacI G_AGCT'C Cuts at: 3931 3931 Size: 6682 SalI G'TCGA_C Cuts at: 1936 4024 1936 Size: 2088 4594 SapI GCTCTTCn'nnn_ Cuts at: 2465 2465 Size: 6682 SbfI CC_TGCA'GG Cuts at: 4034 4034 Size: 6682 ScaI AGT'ACT Cuts at: 4930 6642 4930 Size: 1712 4970 SfcI C'TryA_G Cuts at: 826 1045 2214 3013 3034 3874 4030 5165 5846 Size: 219 1169 799 21 840 156 1135 681 Cuts at: 5846 6037 826 Size: 191 1471 Fragments arranged by size: 1471 1169 1135 840 799 681 219 191 156 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6682 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6682 SmaI CCC'GGG Cuts at: 1872 4009 1872 Size: 2137 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 4786 5687 5949 820 Size: 197 926 153 2690 901 262 1553 Fragments arranged by size: 2690 1553 926 901 262 197 153 SnaBI TAC'GTA Cuts at: 493 3850 4473 493 Size: 3357 623 2702 Fragments arranged by size: 3357 2702 623 SpeI A'CTAG_T Cuts at: 152 152 Size: 6682 SphI G_CATG'C Cuts at: 1132 4040 1132 Size: 2908 3774 SspI AAT'ATT Cuts at: 5 52 4606 5 Size: 47 4554 2081 Fragments arranged by size: 4554 2081 47 StuI AGG'CCT Cuts at: 1517 2948 3088 3172 3368 3424 3452 3508 3676 Size: 1431 140 84 196 56 28 56 168 Cuts at: 3676 1517 Size: 4523 Fragments arranged by size: 4523 1431 196 168 140 84 56 56 28 StyI C'CwwG_G Cuts at: 513 1065 2004 2644 4121 513 Size: 552 939 640 1477 3074 Fragments arranged by size: 3074 1477 939 640 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2286 4709 4726 4879 5064 5509 325 Size: 1255 706 2423 17 153 185 445 1498 Fragments arranged by size: 2423 1498 1255 706 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2067 2477 3562 Size: 274 80 33 51 1443 90 410 1085 Cuts at: 3562 4928 6640 96 Size: 1366 1712 138 Fragments arranged by size: 1712 1443 1366 1085 410 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2097 2129 6642 6674 2097 Size: 32 4513 32 2105 Fragments arranged by size: 4513 2105 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6682 XbaI T'CTAG_A Cuts at: 4018 4018 Size: 6682 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6682 XmaI C'CCGG_G Cuts at: 1870 4007 1870 Size: 2137 4545 XmnI GAAnn'nnTTC Cuts at: 2435 2696 2948 3452 4811 2435 Size: 261 252 504 1359 4306 Fragments arranged by size: 4306 1359 504 261 252 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4490 276 Size: 53 83 186 936 2956 2468 Fragments arranged by size: 2956 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI