Restriction map for
pFN21AB6892
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AflII AflIII
AgeI AhdI Alw44I AlwI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
ClaI CpoI DpnII DraI DsaI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MaeIII MamI MboI
MfeI MflI MluI MroI MslI
MspA1I MunI MvnI NarI NcoI
NdeI NdeII NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI Tsp45I Tsp509I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 486 GACGCC G[GA]^CG[CT]C
AfaI 559 GTAC GT^AC
AocI 591 CCTCAGG CC^T[ACGT]AGG
Aor51HI 263 AGCGCT AGC^GCT
ApaI 477 GGGCCC GGGCC^C
BalI 320 TGGCCA TGG^CCA
BbsI 88 GTCTTC GAAGAC(2/6)
BcgI 485 CGACGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 54 GGATAC GTATCC(6/5)
BglI 322 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 163 CTGGAG CTGGAG(16/14)
BpuAI 88 GTCTTC GAAGAC(2/6)
BsaHI 486 GACGCC G[GA]^CG[CT]C
BseRI 70 GAGGAG GAGGAG(10/8)
BsmAI 73 GAGAC GTCTC(1/5)
BspMI 46 ACCTGC ACCTGC(4/8)
BsrFI 370 GCCGGC [GA]^CCGG[CT]
BstXI 44 CCACCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 591 CCTCAGG CC^T[ACGT]AGG
Cfr10I 370 GCCGGC [GA]^CCGG[CT]
DraIII 62 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 79 GACCAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 263 AGCGCT AGC^GCT
Eco81I 591 CCTCAGG CC^T[ACGT]AGG
FseI 369 GGCCGGCC GGCCGG^CC
Hin1I 486 GACGCC G[GA]^CG[CT]C
HphI 61 TCACC GGTGA(8/7)
MluNI 320 TGGCCA TGG^CCA
MscI 320 TGGCCA TGG^CCA
MseI 40 TTAA T^TAA
NaeI 370 GCCGGC GCC^GGC
NgoMIV 370 GCCGGC G^CCGGC
PflMI 149 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 459 GAGTC GAGTC(4/5)
RsaI 559 GTAC GT^AC
SfaNI 350 GCATC GCATC(5/9)
TfiI 138 GATTC G^A[AT]TC
Tru9I 40 TTAA T^TAA
Van91I 149 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 323 CCAGCCTGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 538 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
548 CAGCCTCTG
AvaII 97 GGTCC G^G[AT]CC
159 GGACC
BanII 442 GGGCTC G[GA]GC[CT]^C
477 GGGCCC
BcnI 134 CCCGG CC^[GC]GG
440 CCGGG
BmyI 442 GGGCTC G[AGT]GC[ACT]^C
477 GGGCCC
Bsp1286I 442 GGGCTC G[AGT]GC[ACT]^C
477 GGGCCC
BsrI 155 ACTGG ACTGG(1/-1)
65 CCAGT
BstF5I 351 CATCC GGATG(2/0)
315 GGATG
DraII 96 GGGTCCC [GA]G^G[ACGT]CC[CT]
158 GGGACCT
EaeI 320 TGGCCA [CT]^GGCC[GA]
368 TGGCCG
EarI 205 GAAGAG CTCTTC(1/4)
223 GAAGAG
EcoO109I 96 GGGTCCC [GA]G^G[ACGT]CC[CT]
158 GGGACCT
FokI 351 CATCC GGATG(9/13)
315 GGATG
HaeII 263 AGCGCT [GA]GCGC^[CT]
540 GGCGCT
HgaI 486 GACGC GACGC(5/10)
565 GACGC
HinfI 138 GATTC G^A[ACGT]TC
459 GAGTC
Ksp632I 205 GAAGAG CTCTTC(1/4)
223 GAAGAG
MwoI 322 GCCAGCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
541 GCGCTGGCAGC
NciI 134 CCCGG CC^[GC]GG
440 CCGGG
PpuMI 96 GGGTCCC [GA]G^G[AT]CC[CT]
158 GGGACCT
StuI 519 AGGCCT AGG^CCT
598 AGGCCT
TspRI 66 CAGTG CA[GC]TG[ACGT][ACGT]^
154 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BsmFI BssKI BstNI Cac8I CfoI
Cfr13I DdeI EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MboII MnlI MspI MvaI NlaIV
Sau96I ScrFI TseI