Restriction map for
pFN21AB6892
(Linear) (Six-base) MAPSORT of: KIBB6892.seq Check: 9635 from: 1 to: 606
KIBB6892.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 55 606
Size: 55 551
AfeI AGC'GCT
Cuts at: 0 265 606
Size: 265 341
AlwNI CAG_nnn'CTG
Cuts at: 0 543 553 606
Size: 543 10 53
Fragments arranged by size:
543 53 10
ApaI G_GGCC'C
Cuts at: 0 481 606
Size: 481 125
BanII G_rGCy'C
Cuts at: 0 446 481 606
Size: 446 35 125
Fragments arranged by size:
446 125 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 81 606
Size: 81 525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 474 508 606
Size: 474 34 98
Fragments arranged by size:
474 98 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 48 606
Size: 48 558
BglI GCCn_nnn'nGGC
Cuts at: 0 328 606
Size: 328 278
Bme1580I G_kGCm'C
Cuts at: 0 481 606
Size: 481 125
BmrI ACTGGGnnnn_n'
Cuts at: 0 59 165 606
Size: 59 106 441
Fragments arranged by size:
441 106 59
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 606
Size: 184 422
BsaHI Gr'CG_yC
Cuts at: 0 487 606
Size: 487 119
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 606
Size: 85 521
Bsp1286I G_dGCh'C
Cuts at: 0 446 481 606
Size: 446 35 125
Fragments arranged by size:
446 125 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 55 606
Size: 55 551
BsrFI r'CCGG_y
Cuts at: 0 370 606
Size: 370 236
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 606
Size: 51 555
Bsu36I CC'TnA_GG
Cuts at: 0 592 606
Size: 592 14
DraIII CAC_nnn'GTG
Cuts at: 0 67 606
Size: 67 539
DrdI GACnn_nn'nnGTC
Cuts at: 0 85 606
Size: 85 521
EaeI y'GGCC_r
Cuts at: 0 320 368 606
Size: 320 48 238
Fragments arranged by size:
320 238 48
EarI CTCTTCn'nnn_
Cuts at: 0 200 218 606
Size: 200 18 388
Fragments arranged by size:
388 200 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 606
Size: 184 422
EcoO109I rG'GnC_Cy
Cuts at: 0 97 159 606
Size: 97 62 447
Fragments arranged by size:
447 97 62
FseI GG_CCGG'CC
Cuts at: 0 374 606
Size: 374 232
HaeII r_GCGC'y
Cuts at: 0 267 544 606
Size: 267 277 62
Fragments arranged by size:
277 267 62
MscI TGG'CCA
Cuts at: 0 322 606
Size: 322 284
NaeI GCC'GGC
Cuts at: 0 372 606
Size: 372 234
NgoMIV G'CCGG_C
Cuts at: 0 370 606
Size: 370 236
PflMI CCAn_nnn'nTGG
Cuts at: 0 155 606
Size: 155 451
PpuMI rG'GwC_Cy
Cuts at: 0 97 159 606
Size: 97 62 447
Fragments arranged by size:
447 97 62
PspOMI G'GGCC_C
Cuts at: 0 477 606
Size: 477 129
SanDI GG'GwC_CC
Cuts at: 0 97 606
Size: 97 509
StuI AGG'CCT
Cuts at: 0 521 600 606
Size: 521 79 6
Fragments arranged by size:
521 79 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 78 606
Size: 78 528
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 181 196 213 228 606
Size: 181 15 17 15 378
Fragments arranged by size:
378 181 17 15 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 330 606
Size: 330 276
Enzymes that do cut:
AarI AfeI AlwNI ApaI BanII BbsI BcgI BciVI
BglI Bme1580I BmrI BpmI BsaHI BseRI Bsp1286I BspMI
BsrFI BstXI Bsu36I DraIII DrdI EaeI EarI Eco57MI
EcoO109I FseI HaeII MscI NaeI NgoMIV PflMI PpuMI
PspOMI SanDI StuI TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI MspA1I NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI ZraI