(Circular) (Six-base) MAPSORT of: pFN21AB6173.seq Check: 5577 from: 1 to: 6664 pFN21AB6173 6664 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2786 2786 Size: 6664 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4474 278 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4007 1937 Size: 2070 4594 Acc65I G'GTAC_C Cuts at: 3985 3985 Size: 6664 AclI AA'CG_TT Cuts at: 1563 4791 5164 1563 Size: 3228 373 3063 Fragments arranged by size: 3228 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2424 2875 3451 4727 6111 6379 1632 Size: 792 451 576 1276 1384 268 1917 Fragments arranged by size: 1917 1384 1276 792 576 451 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6467 AflIII A'CryG_T Cuts at: 1203 3397 5563 6540 1203 Size: 2194 2166 977 1327 Fragments arranged by size: 2194 2166 1327 977 AgeI A'CCGG_T Cuts at: 5495 5495 Size: 6664 AhdI GACnn_n'nnGTC Cuts at: 5393 5393 Size: 6664 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6664 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2838 2848 1184 Size: 1654 10 5000 Fragments arranged by size: 5000 1654 10 AlwNI CAG_nnn'CTG Cuts at: 2605 3748 4407 5979 2605 Size: 1143 659 1572 3290 Fragments arranged by size: 3290 1572 1143 659 ApaI G_GGCC'C Cuts at: 2921 2921 Size: 6664 ApaLI G'TGCA_C Cuts at: 2637 4721 5877 2637 Size: 2084 1156 3424 Fragments arranged by size: 3424 2084 1156 ApoI r'AATT_y Cuts at: 1493 2238 3022 3333 3405 3501 3973 4199 4351 Size: 745 784 311 72 96 472 226 152 Cuts at: 4351 6460 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 784 745 472 311 226 152 96 72 AseI AT'TA_AT Cuts at: 160 1032 5218 160 Size: 872 4186 1606 Fragments arranged by size: 4186 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 AvaI C'yCGr_G Cuts at: 1870 1943 2529 2786 3989 1870 Size: 73 586 257 1203 4545 Fragments arranged by size: 4545 1203 586 257 73 AvrII C'CTAG_G Cuts at: 2632 2632 Size: 6664 BamHI G'GATC_C Cuts at: 2293 3994 4361 2293 Size: 1701 367 4596 Fragments arranged by size: 4596 1701 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2771 2967 3912 3985 5440 Size: 325 217 627 984 196 945 73 1455 Cuts at: 5440 618 Size: 1842 Fragments arranged by size: 1842 1455 984 945 627 325 217 196 73 BanII G_rGCy'C Cuts at: 1391 2274 2921 3647 3815 3899 3983 1391 Size: 883 647 726 168 84 84 4072 Fragments arranged by size: 4072 883 726 647 168 84 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5693 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2777 2811 4855 4889 1903 Size: 34 840 34 2044 34 3678 Fragments arranged by size: 3678 2044 840 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2274 4556 5766 2274 Size: 2282 1210 3172 Fragments arranged by size: 3172 2282 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6664 BfrBI ATG'CAT Cuts at: 3863 3863 Size: 6664 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5275 136 Size: 107 122 71 631 605 3603 1525 Fragments arranged by size: 3603 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6659 6659 Size: 6664 BlpI GC'TnA_GC Cuts at: 3730 3768 4081 3730 Size: 38 313 6313 Fragments arranged by size: 6313 313 38 Bme1580I G_kGCm'C Cuts at: 1790 2641 2774 2921 2970 3915 4725 5881 1790 Size: 851 133 147 49 945 810 1156 2573 Fragments arranged by size: 2573 1156 945 851 810 147 133 49 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6664 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2075 2427 2831 Size: 416 53 83 399 24 658 352 404 Cuts at: 2831 4395 5353 442 Size: 1564 958 1753 Fragments arranged by size: 1753 1564 958 658 416 404 399 352 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6664 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1996 2156 2258 2627 2642 2975 3245 3699 Size: 757 160 102 369 15 333 270 454 Cuts at: 3699 5324 1239 Size: 1625 2579 Fragments arranged by size: 2579 1625 757 454 369 333 270 160 102 15 Bpu10I CC'TnA_GC Cuts at: 2268 3171 2268 Size: 903 5761 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2229 3061 4789 5654 5952 2229 Size: 832 1728 865 298 2941 Fragments arranged by size: 2941 1728 865 832 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2161 2951 2980 3679 5327 882 Size: 617 662 790 29 699 1648 2219 Fragments arranged by size: 2219 1648 790 699 662 617 29 BsaAI yAC'GTr Cuts at: 493 1204 3398 4455 493 Size: 711 2194 1057 2702 Fragments arranged by size: 2702 2194 1057 711 BsaBI GATnn'nnATC Cuts at: 6658 6658 Size: 6664 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4471 4853 5488 275 Size: 53 83 186 936 2938 382 635 1451 Fragments arranged by size: 2938 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2655 3552 3593 5097 5495 5769 5916 6327 2655 Size: 897 41 1504 398 274 147 411 2992 Fragments arranged by size: 2992 1504 897 411 398 274 147 41 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6439 Fragments arranged by size: 6439 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 2750 2822 2870 2915 1186 Size: 1033 531 72 48 45 4935 Fragments arranged by size: 4935 1033 531 72 48 45 BseYI C'CCAG_C Cuts at: 2799 2914 3053 3741 5867 6363 2799 Size: 115 139 688 2126 496 3100 Fragments arranged by size: 3100 2126 688 496 139 115 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2442 1163 Size: 573 706 5385 Fragments arranged by size: 5385 706 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4114 4875 5903 1540 Size: 275 186 2113 761 1028 2301 Fragments arranged by size: 2301 2113 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2274 2641 2850 3238 3647 3815 3899 4725 4810 Size: 367 209 388 409 168 84 826 85 Cuts at: 4810 5881 2274 Size: 1071 3057 Fragments arranged by size: 3057 1071 826 409 388 367 209 168 85 84 BsmI GAATG_Cn' Cuts at: 2383 4175 4268 2383 Size: 1792 93 4779 Fragments arranged by size: 4779 1792 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2274 2641 2774 2850 2921 2970 3238 Size: 399 484 367 133 76 71 49 268 Cuts at: 3238 3647 3815 3899 3915 3983 4725 4810 5881 Size: 409 168 84 16 68 742 85 1071 Cuts at: 5881 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 484 409 399 367 268 168 133 85 84 76 71 68 49 16 BspEI T'CCGG_A Cuts at: 2655 3552 6327 2655 Size: 897 2775 2992 Fragments arranged by size: 2992 2775 897 BspHI T'CATG_A Cuts at: 4553 6283 4553 Size: 1730 4934 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2431 2770 2786 4019 844 Size: 1587 339 16 1233 3489 Fragments arranged by size: 3489 1587 1233 339 16 BsrBI CCG'CTC Cuts at: 1394 1823 4551 1394 Size: 429 2728 3507 Fragments arranged by size: 3507 2728 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3454 5159 5333 65 Size: 1183 2206 1705 174 1396 Fragments arranged by size: 2206 1705 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5308 5495 1700 Size: 110 3498 187 2869 Fragments arranged by size: 3498 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2118 96 Size: 2022 4642 BssHII G'CGCG_C Cuts at: 1925 2483 1925 Size: 558 6106 BssSI C'ACGA_G Cuts at: 4724 5736 4724 Size: 1012 5652 BstAPI GCAn_nnn'nTGC Cuts at: 794 2778 794 Size: 1984 4680 BstBI TT'CG_AA Cuts at: 5468 5468 Size: 6664 BstEII G'GTnAC_C Cuts at: 1184 2468 1184 Size: 1284 5380 BstYI r'GATC_y Cuts at: 1972 2293 2385 2601 3994 4361 4746 4763 6215 Size: 321 92 216 1393 367 385 17 1452 Cuts at: 6215 6301 6313 6659 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1393 385 367 346 321 216 92 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2475 2580 2853 3036 3749 2475 Size: 105 273 183 713 5390 Fragments arranged by size: 5390 713 273 183 105 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5173 Fragments arranged by size: 5173 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2958 487 Size: 683 56 1732 4193 Fragments arranged by size: 4193 1732 683 56 BtsI GCAGTG_nn' Cuts at: 2639 2793 3538 4180 4974 4994 2639 Size: 154 745 642 794 20 4309 Fragments arranged by size: 4309 794 745 642 154 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6664 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3856 3891 529 Size: 35 3292 35 3302 Fragments arranged by size: 3302 3292 35 35 DraI TTT'AAA Cuts at: 3968 3968 Size: 6664 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2029 2728 5671 809 Size: 507 713 699 2943 1802 Fragments arranged by size: 2943 1802 713 699 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2222 4111 5000 Size: 54 1425 50 275 18 392 1889 889 Cuts at: 5000 6629 8 Size: 1629 43 Fragments arranged by size: 1889 1629 1425 889 392 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4111 1537 Size: 275 2299 4090 Fragments arranged by size: 4090 2299 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2304 3410 3652 4594 1353 Size: 723 228 1106 242 942 3423 Fragments arranged by size: 3423 1106 942 723 242 228 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3794 5251 5625 5771 387 Size: 1064 138 2205 1457 374 146 1280 Fragments arranged by size: 2205 1457 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2272 3645 3813 3897 2272 Size: 1373 168 84 5039 Fragments arranged by size: 5039 1373 168 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1996 2156 2258 2424 2627 2642 2875 Size: 393 364 160 102 166 203 15 233 Cuts at: 2875 2975 3245 3451 3699 4727 5324 6111 6379 Size: 100 270 206 248 1028 597 787 268 Cuts at: 6379 1239 Size: 1524 Fragments arranged by size: 1524 1028 787 597 393 364 270 268 248 233 206 203 166 160 102 100 15 EcoNI CCTnn'n_nnAGG Cuts at: 2711 2711 Size: 6664 EcoO109I rG'GnC_Cy Cuts at: 2341 2392 2525 2536 3603 3714 2341 Size: 51 133 11 1067 111 5291 Fragments arranged by size: 5291 1067 133 111 51 11 EcoRI G'AATT_C Cuts at: 2238 3501 3973 4351 2238 Size: 1263 472 378 4551 Fragments arranged by size: 4551 1263 472 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3217 3217 Size: 6664 FspI TGC'GCA Cuts at: 3356 5170 3356 Size: 1814 4850 HaeII r_GCGC'y Cuts at: 1678 3303 3768 3856 5811 1678 Size: 1625 465 88 1955 2531 Fragments arranged by size: 2531 1955 1625 465 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 5305 5337 5379 Size: 32 135 32 605 32 3096 32 42 Cuts at: 5379 5411 1373 Size: 32 2626 Fragments arranged by size: 3096 2626 605 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4008 4254 669 Size: 988 281 2070 246 3079 Fragments arranged by size: 3079 2070 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6664 HpaI GTT'AAC Cuts at: 4254 4254 Size: 6664 KpnI G_GTAC'C Cuts at: 3989 3989 Size: 6664 MfeI C'AATT_G Cuts at: 4263 4263 Size: 6664 MluI A'CGCG_T Cuts at: 6540 6540 Size: 6664 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2045 2470 3452 5778 5962 1305 Size: 740 425 982 2326 184 2007 Fragments arranged by size: 2326 2007 982 740 425 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2224 10 Size: 54 1425 343 392 4450 Fragments arranged by size: 4450 1425 392 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2466 3694 3862 4622 4981 5140 518 Size: 648 1300 1228 168 760 359 159 2042 Fragments arranged by size: 2042 1300 1228 760 648 359 168 159 MspA1I CmG'CkG Cuts at: 1606 2278 4080 4757 5905 6150 1606 Size: 672 1802 677 1148 245 2120 Fragments arranged by size: 2120 1802 1148 677 672 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5173 Fragments arranged by size: 5173 939 552 NdeI CA'TA_TG Cuts at: 387 3859 387 Size: 3472 3192 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6664 NotI GC'GGCC_GC Cuts at: 4111 4111 Size: 6664 NsiI A_TGCA'T Cuts at: 3865 3865 Size: 6664 NspI r_CATG'y Cuts at: 1132 1229 3867 4022 5567 1132 Size: 97 2638 155 1545 2229 Fragments arranged by size: 2638 2229 1545 155 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6664 PciI A'CATG_T Cuts at: 5563 5563 Size: 6664 PflMI CCAn_nnn'nTGG Cuts at: 1108 2362 2698 2878 1108 Size: 1254 336 180 4894 Fragments arranged by size: 4894 1254 336 180 PfoI T'CCnGG_A Cuts at: 2604 2871 3129 2604 Size: 267 258 6139 Fragments arranged by size: 6139 267 258 PmeI GTTT'AAAC Cuts at: 3968 3968 Size: 6664 PmlI CAC'GTG Cuts at: 3398 3398 Size: 6664 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2141 2173 4733 4765 6246 6278 2141 Size: 32 2560 32 1481 32 2527 Fragments arranged by size: 2560 2527 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2341 2525 3714 2341 Size: 184 1189 5291 Fragments arranged by size: 5291 1189 184 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6664 PsiI TTA'TAA Cuts at: 2511 3497 4234 2511 Size: 986 737 4941 Fragments arranged by size: 4941 986 737 PspOMI G'GGCC_C Cuts at: 2917 2917 Size: 6664 PspXI vC'TCGA_Gb Cuts at: 2786 2786 Size: 6664 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3548 3580 3548 Size: 32 6632 PstI C_TGCA'G Cuts at: 830 2755 4016 830 Size: 1925 1261 3478 Fragments arranged by size: 3478 1925 1261 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6664 PvuII CAG'CTG Cuts at: 2278 2278 Size: 6664 SacI G_AGCT'C Cuts at: 2274 3647 3815 3899 2274 Size: 1373 168 84 5039 Fragments arranged by size: 5039 1373 168 84 SalI G'TCGA_C Cuts at: 1936 4006 1936 Size: 2070 4594 SapI GCTCTTCn'nnn_ Cuts at: 3410 3652 3410 Size: 242 6422 SbfI CC_TGCA'GG Cuts at: 2755 4016 2755 Size: 1261 5403 ScaI AGT'ACT Cuts at: 2745 3478 4912 6624 2745 Size: 733 1434 1712 2785 Fragments arranged by size: 2785 1712 1434 733 SexAI A'CCwGG_T Cuts at: 2151 2970 2151 Size: 819 5845 SfcI C'TryA_G Cuts at: 826 1045 2143 2573 2751 4012 5147 5828 6019 Size: 219 1098 430 178 1261 1135 681 191 Cuts at: 6019 826 Size: 1471 Fragments arranged by size: 1471 1261 1135 1098 681 430 219 191 178 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6664 SmaI CCC'GGG Cuts at: 1872 2531 3991 1872 Size: 659 1460 4545 Fragments arranged by size: 4545 1460 659 SmlI C'TyrA_G Cuts at: 820 1017 1943 2244 2786 3076 4768 5669 5931 Size: 197 926 301 542 290 1692 901 262 Cuts at: 5931 820 Size: 1553 Fragments arranged by size: 1692 1553 926 901 542 301 290 262 197 SnaBI TAC'GTA Cuts at: 493 4455 493 Size: 3962 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6664 SphI G_CATG'C Cuts at: 1132 3867 4022 1132 Size: 2735 155 3774 Fragments arranged by size: 3774 2735 155 SspI AAT'ATT Cuts at: 5 52 3549 4588 5 Size: 47 3497 1039 2081 Fragments arranged by size: 3497 2081 1039 47 StuI AGG'CCT Cuts at: 1517 3884 1517 Size: 2367 4297 StyI C'CwwG_G Cuts at: 513 1065 2004 2592 2632 3438 4103 513 Size: 552 939 588 40 806 665 3074 Fragments arranged by size: 3074 939 806 665 588 552 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2653 2694 2948 3905 4691 4708 4861 Size: 1255 1073 41 254 957 786 17 153 Cuts at: 4861 5046 5491 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1073 957 786 445 254 185 153 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 2743 3064 Size: 274 80 33 51 1443 141 625 321 Cuts at: 3064 3476 4910 6622 96 Size: 412 1434 1712 138 Fragments arranged by size: 1712 1443 1434 625 412 321 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6624 6656 6624 Size: 32 6632 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6664 XbaI T'CTAG_A Cuts at: 4000 4000 Size: 6664 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 2363 3227 3293 2185 Size: 178 864 66 5556 Fragments arranged by size: 5556 864 178 66 XhoI C'TCGA_G Cuts at: 1943 2786 1943 Size: 843 5821 XmaI C'CCGG_G Cuts at: 1870 2529 3989 1870 Size: 659 1460 4545 Fragments arranged by size: 4545 1460 659 XmnI GAAnn'nnTTC Cuts at: 2745 3464 4793 2745 Size: 719 1329 4616 Fragments arranged by size: 4616 1329 719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4472 276 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI RsrII SacII SanDI SfiI SfoI SrfI SwaI