Restriction map for
pFN21AB6829
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MfeI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 201 GAATTC [GA]^AATT[CT]
AlwI 98 GGATC GGATC(4/5)
ApoI 201 GAATTC [GA]^AATT[CT]
AspHI 223 GTGCTC G[AT]GC[AT]^C
AvaII 347 GGTCC G^G[AT]CC
BanII 380 GAGCCC G[GA]GC[CT]^C
BbvI 104 GCTGC GCAGC(8/12)
BcnI 95 CCCGG CC^[GC]GG
BglII 180 AGATCT A^GATCT
BpmI 336 CTGGAG CTGGAG(16/14)
BsaBI 175 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 223 GTGCTC G[AT]GC[AT]^C
BsmFI 348 GTCCC GGGAC(10/14)
BsrI 335 ACTGG ACTGG(1/-1)
BstF5I 450 GGATG GGATG(2/0)
BstYI 180 AGATCT [GA]^GATC[CT]
Cac8I 329 GCTGGC GC[ACGT]^[ACGT]GC
DraII 346 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 283 GACTTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 346 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 201 GAATTC G^AATTC
EcoT14I 60 CCAAGG C^C[AT][AT]GG
FokI 450 GGATG GGATG(9/13)
HapII 96 CCGG C^CGG
HinfI 434 GATTC G^A[ACGT]TC
HpaII 96 CCGG C^CGG
HphI 227 TCACC GGTGA(8/7)
MamI 175 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 180 AGATCT [GA]^GATC[CT]
MspI 96 CCGG C^CGG
NciI 95 CCCGG CC^[GC]GG
PpuMI 346 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 102 CAGCTG CAG^CTG
SexAI 212 ACCTGGT A^CC[AT]GGT
SfaNI 175 GATGC GCATC(5/9)
SmlI 423 CTCAAG C^T[CT][GA]AG
StyI 60 CCAAGG C^C[AT][AT]GG
TfiI 434 GATTC G^A[AT]TC
TseI 104 GCTGC G^C[AT]GC
Tsp509I 202 AATT ^AATT
XhoII 180 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 324 CCGC CCGC(-3/-1)
327 CCGC
AluI 13 AGCT AG^CT
103 AGCT
BanI 115 GGCACC G^G[CT][GA]CC
241 GGTGCC
BbsI 154 GAAGAC GAAGAC(2/6)
463 GAAGAC
BglI 107 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
244 GCCTTTGTGGC
BmyI 223 GTGCTC G[AGT]GC[ACT]^C
380 GAGCCC
BpuAI 154 GAAGAC GAAGAC(2/6)
463 GAAGAC
BsaI 163 GAGACC GGTCTC(1/5)
354 GGTCTC
BsiYI 89 CCCAGGCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
231 CCAGGCCGAGG
BslI 89 CCCAGGCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
231 CCAGGCCGAGG
Bsp1286I 223 GTGCTC G[AGT]GC[ACT]^C
380 GAGCCC
DdeI 5 CTCAG C^T[ACGT]AG
53 CTCAG
Fnu4HI 104 GCTGC GC^[ACGT]GC
326 GCCGC
ItaI 104 GCTGC GC^[ACGT]GC
326 GCCGC
MboII 154 GAAGA GAAGA(8/7)
463 GAAGA
MspA1I 102 CAGCTG C[AC]G^C[GT]G
327 CCGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
BsaJI BsmAI BssKI BstNI Cfr13I
DpnII EcoRII HaeIII MboI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI Tru9I
TspRI