(Linear) (Six-base) MAPSORT of: KIBB6829.seq  Check: 9813  from: 1  to: 498



KIBB6829.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

ApoI r'AATT_y

Cuts at:      0     201     498 
   Size:        201     297
 
BanI G'GyrC_C

Cuts at:      0     115     241     498 
   Size:        115     126     257

  Fragments arranged by size: 

                 257     126     115
 
BanII G_rGCy'C

Cuts at:      0     384     498 
   Size:        384     114
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     161     470     498 
   Size:        161     309      28

  Fragments arranged by size: 

                 309     161      28
 
BglI GCCn_nnn'nGGC

Cuts at:      0     113     250     498 
   Size:        113     137     248

  Fragments arranged by size: 

                 248     137     113
 
BglII A'GATC_T

Cuts at:      0     180     498 
   Size:        180     318
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     357     498 
   Size:        357     141
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     408     498 
   Size:        408      90
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     157     360     498 
   Size:        157     203     138

  Fragments arranged by size: 

                 203     157     138
 
BsaBI GATnn'nnATC

Cuts at:      0     179     498 
   Size:        179     319
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     457     487     498 
   Size:        457      30      11

  Fragments arranged by size: 

                 457      30      11
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     498 
   Size:        227     271
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     384     498 
   Size:        227     157     114

  Fragments arranged by size: 

                 227     157     114
 
BstYI r'GATC_y

Cuts at:      0     180     498 
   Size:        180     318
 
BtsI GCAGTG_nn'

Cuts at:      0     348     396     498 
   Size:        348      48     102

  Fragments arranged by size: 

                 348     102      48
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     289     498 
   Size:        289     209
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     357     498 
   Size:        357     141
 
EcoO109I rG'GnC_Cy

Cuts at:      0     347     498 
   Size:        347     151
 
EcoRI G'AATT_C

Cuts at:      0     201     498 
   Size:        201     297
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     492     498 
   Size:        492       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     457     489     498 
   Size:        457      32       9

  Fragments arranged by size: 

                 457      32       9
 
MspA1I CmG'CkG

Cuts at:      0     104     329     498 
   Size:        104     225     169

  Fragments arranged by size: 

                 225     169     104
 
PpuMI rG'GwC_Cy

Cuts at:      0     347     498 
   Size:        347     151
 
PvuII CAG'CTG

Cuts at:      0     104     498 
   Size:        104     394
 
SanDI GG'GwC_CC

Cuts at:      0     347     498 
   Size:        347     151
 
SexAI A'CCwGG_T

Cuts at:      0     212     498 
   Size:        212     286
 
SmlI C'TyrA_G

Cuts at:      0     423     498 
   Size:        423      75
 
StyI C'CwwG_G

Cuts at:      0      60     498 
   Size:         60     438
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     244     498 
   Size:        244     254
 

 Enzymes that do cut:

     ApoI     BanI    BanII     BbsI     BglI    BglII     BpmI    BpuEI
     BsaI    BsaBI    BsaXI  BsiHKAI Bsp1286I    BstYI     BtsI     DrdI
  Eco57MI EcoO109I    EcoRI     FalI    Hin4I   MspA1I    PpuMI    PvuII
    SanDI    SexAI     SmlI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI