(Circular) (Six-base) MAPSORT of: pFN21AB6159.seq Check: 2587 from: 1 to: 6502 pFN21AB6159 6502 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4312 278 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2872 3845 1937 Size: 935 973 4594 Fragments arranged by size: 4594 973 935 Acc65I G'GTAC_C Cuts at: 2182 3823 2182 Size: 1641 4861 AclI AA'CG_TT Cuts at: 1563 4629 5002 1563 Size: 3066 373 3063 Fragments arranged by size: 3066 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2007 2375 4565 5949 6217 1632 Size: 375 368 2190 1384 268 1917 Fragments arranged by size: 2190 1917 1384 375 368 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6305 AflIII A'CryG_T Cuts at: 1203 2383 3651 3799 5401 6378 1203 Size: 1180 1268 148 1602 977 1327 Fragments arranged by size: 1602 1327 1268 1180 977 148 AgeI A'CCGG_T Cuts at: 5333 5333 Size: 6502 AhdI GACnn_n'nnGTC Cuts at: 5231 5231 Size: 6502 AleI CACnn'nnGTG Cuts at: 1166 3132 1166 Size: 1966 4536 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6502 AlwNI CAG_nnn'CTG Cuts at: 4245 5817 4245 Size: 1572 4930 ApaLI G'TGCA_C Cuts at: 4559 5715 4559 Size: 1156 5346 ApoI r'AATT_y Cuts at: 1493 2437 3811 4037 4189 6298 1493 Size: 944 1374 226 152 2109 1697 Fragments arranged by size: 2109 1697 1374 944 226 152 AseI AT'TA_AT Cuts at: 160 1032 5056 160 Size: 872 4024 1606 Fragments arranged by size: 4024 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 AvaI C'yCGr_G Cuts at: 1870 1943 2782 3827 1870 Size: 73 839 1045 4545 Fragments arranged by size: 4545 1045 839 73 BamHI G'GATC_C Cuts at: 3832 4199 3832 Size: 367 6135 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2182 3823 5278 618 Size: 325 217 627 395 1641 1455 1842 Fragments arranged by size: 1842 1641 1455 627 395 325 217 BanII G_rGCy'C Cuts at: 1391 2649 3821 1391 Size: 1258 1172 4072 Fragments arranged by size: 4072 1258 1172 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5531 BbvCI CC'TCA_GC Cuts at: 2939 2939 Size: 6502 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2107 2141 4693 4727 1903 Size: 34 170 34 2552 34 3678 Fragments arranged by size: 3678 2552 170 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3201 4394 5604 3201 Size: 1193 1210 4099 Fragments arranged by size: 4099 1210 1193 BclI T'GATC_A Cuts at: 1548 1548 Size: 6502 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5113 136 Size: 107 122 71 631 605 3441 1525 Fragments arranged by size: 3441 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2394 3271 6497 2394 Size: 877 3226 2399 Fragments arranged by size: 3226 2399 877 BlpI GC'TnA_GC Cuts at: 2653 3919 2653 Size: 1266 5236 Bme1580I G_kGCm'C Cuts at: 1790 4563 5719 1790 Size: 2773 1156 2573 Fragments arranged by size: 2773 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6502 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2039 2053 4233 Size: 416 53 83 399 24 622 14 2180 Cuts at: 4233 5191 442 Size: 958 1753 Fragments arranged by size: 2180 1753 958 622 416 399 83 53 24 14 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6502 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2668 2668 Size: 6502 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 3176 3405 5162 1239 Size: 1061 876 229 1757 2579 Fragments arranged by size: 2579 1757 1061 876 229 Bpu10I CC'TnA_GC Cuts at: 2939 2939 Size: 6502 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2557 2731 4627 5492 5790 2557 Size: 174 1896 865 298 3269 Fragments arranged by size: 3269 1896 865 298 174 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5165 882 Size: 617 3666 2219 Fragments arranged by size: 3666 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3652 4293 493 Size: 711 2448 641 2702 Fragments arranged by size: 2702 2448 711 641 BsaBI GATnn'nnATC Cuts at: 6496 6496 Size: 6502 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4309 4691 5326 275 Size: 53 83 186 936 2776 382 635 1451 Fragments arranged by size: 2776 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2746 3131 4935 5333 5607 5754 6165 2746 Size: 385 1804 398 274 147 411 3083 Fragments arranged by size: 3083 1804 411 398 385 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6277 Fragments arranged by size: 6277 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2538 2573 3065 3431 3683 1186 Size: 1352 35 492 366 252 4005 Fragments arranged by size: 4005 1352 492 366 252 35 BseYI C'CCAG_C Cuts at: 2585 2648 5705 6201 2585 Size: 63 3057 496 2886 Fragments arranged by size: 3057 2886 496 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2433 2696 2920 3004 3676 1163 Size: 573 697 263 224 84 672 3989 Fragments arranged by size: 3989 697 672 573 263 224 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3952 4713 5741 1540 Size: 275 186 1951 761 1028 2301 Fragments arranged by size: 2301 1951 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4563 4648 5719 4563 Size: 85 1071 5346 Fragments arranged by size: 5346 1071 85 BsmI GAATG_Cn' Cuts at: 2579 4013 4106 2579 Size: 1434 93 4975 Fragments arranged by size: 4975 1434 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2649 3821 4563 4648 5719 1391 Size: 399 859 1172 742 85 1071 2174 Fragments arranged by size: 2174 1172 1071 859 742 399 85 BspEI T'CCGG_A Cuts at: 6165 6165 Size: 6502 BspHI T'CATG_A Cuts at: 4391 6121 4391 Size: 1730 4772 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3857 844 Size: 3013 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3373 4389 1394 Size: 429 1550 1016 3507 Fragments arranged by size: 3507 1550 1016 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4997 5171 65 Size: 1183 3749 174 1396 Fragments arranged by size: 3749 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5146 5333 1700 Size: 110 3336 187 2869 Fragments arranged by size: 3336 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3616 96 Size: 3520 2982 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6502 BssSI C'ACGA_G Cuts at: 3368 4562 5574 3368 Size: 1194 1012 4296 Fragments arranged by size: 4296 1194 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2907 794 Size: 2113 4389 BstBI TT'CG_AA Cuts at: 5306 5306 Size: 6502 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6502 BstXI CCAn_nnnn'nTGG Cuts at: 2454 2454 Size: 6502 BstYI r'GATC_y Cuts at: 1972 2394 3271 3832 4199 4584 4601 6053 6139 Size: 422 877 561 367 385 17 1452 86 Cuts at: 6139 6151 6497 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 877 561 422 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2873 2873 Size: 6502 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5950 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5763 Fragments arranged by size: 5763 683 56 BtsI GCAGTG_nn' Cuts at: 2824 2908 3664 4018 4812 4832 2824 Size: 84 756 354 794 20 4494 Fragments arranged by size: 4494 794 756 354 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6502 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6467 DraI TTT'AAA Cuts at: 2839 3806 2839 Size: 967 5535 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5509 809 Size: 507 4193 1802 Fragments arranged by size: 4193 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3415 3667 3949 Size: 54 1425 50 275 18 1585 252 282 Cuts at: 3949 4838 6467 8 Size: 889 1629 43 Fragments arranged by size: 1629 1585 1425 889 282 275 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3949 1537 Size: 275 2137 4090 Fragments arranged by size: 4090 2137 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2043 2228 2961 4432 1353 Size: 690 185 733 1471 3423 Fragments arranged by size: 3423 1471 733 690 185 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5089 5463 5609 387 Size: 1064 138 3500 374 146 1280 Fragments arranged by size: 3500 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2300 2375 3176 3405 4565 5162 Size: 393 375 293 75 801 229 1160 597 Cuts at: 5162 5949 6217 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1160 801 787 597 393 375 293 268 229 75 EcoNI CCTnn'n_nnAGG Cuts at: 2619 2619 Size: 6502 EcoO109I rG'GnC_Cy Cuts at: 2787 2787 Size: 6502 EcoRI G'AATT_C Cuts at: 2437 3811 4189 2437 Size: 1374 378 4750 Fragments arranged by size: 4750 1374 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3121 3121 Size: 6502 FspI TGC'GCA Cuts at: 5008 5008 Size: 6502 HaeII r_GCGC'y Cuts at: 1678 5649 1678 Size: 3971 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2092 2124 2227 2259 2838 Size: 32 135 32 520 32 103 32 579 Cuts at: 2838 2870 3086 3118 5143 5175 5217 5249 1373 Size: 32 216 32 2025 32 42 32 2626 Fragments arranged by size: 2626 2025 579 520 216 135 103 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2381 3186 3354 3606 3846 4092 Size: 988 281 443 805 168 252 240 246 Cuts at: 4092 669 Size: 3079 Fragments arranged by size: 3079 988 805 443 281 252 246 240 168 HindIII A'AGCT_T Cuts at: 748 2833 3001 3169 748 Size: 2085 168 168 4081 Fragments arranged by size: 4081 2085 168 168 HpaI GTT'AAC Cuts at: 4092 4092 Size: 6502 KpnI G_GTAC'C Cuts at: 2186 3827 2186 Size: 1641 4861 MfeI C'AATT_G Cuts at: 4101 4101 Size: 6502 MluI A'CGCG_T Cuts at: 6378 6378 Size: 6502 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2958 3410 5616 5800 1305 Size: 1653 452 2206 184 2007 Fragments arranged by size: 2206 2007 1653 452 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4680 Fragments arranged by size: 4680 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3132 3300 3552 4460 4819 4978 518 Size: 648 1966 168 252 908 359 159 2042 Fragments arranged by size: 2042 1966 908 648 359 252 168 159 MspA1I CmG'CkG Cuts at: 1606 3918 4595 5743 5988 1606 Size: 2312 677 1148 245 2120 Fragments arranged by size: 2312 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5950 NdeI CA'TA_TG Cuts at: 387 387 Size: 6502 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6502 NotI GC'GGCC_GC Cuts at: 3949 3949 Size: 6502 NspI r_CATG'y Cuts at: 1132 1229 2387 3860 5405 1132 Size: 97 1158 1473 1545 2229 Fragments arranged by size: 2229 1545 1473 1158 97 PasI CC'CwG_GG Cuts at: 1348 2447 1348 Size: 1099 5403 PciI A'CATG_T Cuts at: 2383 5401 2383 Size: 3018 3484 PflMI CCAn_nnn'nTGG Cuts at: 1108 2069 2179 2541 2758 1108 Size: 961 110 362 217 4852 Fragments arranged by size: 4852 961 362 217 110 PmeI GTTT'AAAC Cuts at: 3806 3806 Size: 6502 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4571 4603 6084 6116 4571 Size: 32 1481 32 4957 Fragments arranged by size: 4957 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2787 2787 Size: 6502 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6502 PsiI TTA'TAA Cuts at: 4072 4072 Size: 6502 PstI C_TGCA'G Cuts at: 830 3491 3854 830 Size: 2661 363 3478 Fragments arranged by size: 3478 2661 363 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6502 SalI G'TCGA_C Cuts at: 1936 3844 1936 Size: 1908 4594 SbfI CC_TGCA'GG Cuts at: 3854 3854 Size: 6502 ScaI AGT'ACT Cuts at: 4750 6462 4750 Size: 1712 4790 SfcI C'TryA_G Cuts at: 826 1045 2731 3487 3850 4985 5666 5857 826 Size: 219 1686 756 363 1135 681 191 1471 Fragments arranged by size: 1686 1471 1135 756 681 363 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6502 SmaI CCC'GGG Cuts at: 1872 3829 1872 Size: 1957 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2572 2710 4606 5507 5769 820 Size: 197 926 629 138 1896 901 262 1553 Fragments arranged by size: 1896 1553 926 901 629 262 197 138 SnaBI TAC'GTA Cuts at: 493 4293 493 Size: 3800 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6502 SphI G_CATG'C Cuts at: 1132 3860 1132 Size: 2728 3774 SspI AAT'ATT Cuts at: 5 52 4426 5 Size: 47 4374 2081 Fragments arranged by size: 4374 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6502 StyI C'CwwG_G Cuts at: 513 1065 3941 513 Size: 552 2876 3074 Fragments arranged by size: 3074 2876 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4529 4546 4699 4884 5329 325 Size: 1255 2949 17 153 185 445 1498 Fragments arranged by size: 2949 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2386 3616 4748 Size: 274 80 33 51 1443 409 1230 1132 Cuts at: 4748 6460 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1230 1132 409 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6462 6494 6462 Size: 32 6470 Tth111I GACn'n_nGTC Cuts at: 1316 2241 1316 Size: 925 5577 XbaI T'CTAG_A Cuts at: 3838 3838 Size: 6502 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6502 XmaI C'CCGG_G Cuts at: 1870 3827 1870 Size: 1957 4545 XmnI GAAnn'nnTTC Cuts at: 2959 4631 2959 Size: 1672 4830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4310 276 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI