Restriction map for
pFN21AB6292
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
AscI AseI AsnI AspEI AspI
AviII AvrII BalI BbrPI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KspI MaeI MfeI
MluNI MroI MscI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI Tth111I Van91I
XbaI XcmI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 2322 GGTACC G^GTACC
AccI 1228 GTCTAC GT^[AC][GT]AC
AccII 1026 CGCG CG^CG
AclI 1624 AACGTT AA^CGTT
AcsI 2165 AAATTC [GA]^AATT[CT]
AlwNI 41 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2249 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1168 AGCGCT AGC^GCT
ApoI 2165 AAATTC [GA]^AATT[CT]
Asp700I 508 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2322 GGTACC G^GTACC
BamHI 2361 GGATCC G^GATCC
BbvCI 1493 CCTCAGC CCTCAGC(-5/-2)
BglI 1456 GCCGCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 855 CACGTA [CT]AC^GT[GA]
BsaBI 429 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 64 TGTACA T^GTACA
BspHI 2288 TCATGA T^CATGA
BspLU11I 1470 ACATGT A^CATGT
BsrBI 1942 GAGCGG CCGCTC(-3/-3)
BsrGI 64 TGTACA T^GTACA
BstUI 1026 CGCG CG^CG
Bsu36I 2249 CCTCAGG CC^T[ACGT]AGG
Eco47III 1168 AGCGCT AGC^GCT
Eco81I 2249 CCTCAGG CC^T[ACGT]AGG
EcoNI 137 CCTTACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 824 AAGCTT A^AGCTT
KpnI 2322 GGTACC GGTAC^C
MaeIII 2099 GTGAC ^GT[ACGT]AC
MamI 429 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1025 ACGCGT A^CGCGT
MspA1I 1585 CCGCTG C[AC]G^C[GT]G
MvnI 1026 CGCG CG^CG
NspI 1470 ACATGT [GA]CATG^[CT]
PaeR7I 2116 CTCGAG C^TCGAG
Psp1406I 1624 AACGTT AA^CGTT
PstI 1744 CTGCAG CTGCA^G
RcaI 2288 TCATGA T^CATGA
SexAI 1757 ACCTGGT A^CC[AT]GGT
SspBI 64 TGTACA T^GTACA
SspI 290 AATATT AAT^ATT
StuI 1415 AGGCCT AGG^CCT
Tsp45I 2099 GTGAC ^GT[GC]AC
XhoI 2116 CTCGAG C^TCGAG
XmnI 508 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 1362 GGCGCC G[GA]^CG[CT]C
2034 GGCGCC
Alw44I 1678 GTGCAC G^TGCAC
1906 GTGCAC
ApaI 797 GGGCCC GGGCC^C
981 GGGCCC
ApaLI 1678 GTGCAC G^TGCAC
1906 GTGCAC
BbeI 1362 GGCGCC GGCGC^C
2034 GGCGCC
BbsI 743 GAAGAC GAAGAC(2/6)
811 GAAGAC
BcgI 1292 CGAGCTCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1235 GCAATCTCCTCG
BglII 2055 AGATCT A^GATCT
2277 AGATCT
BpuAI 743 GAAGAC GAAGAC(2/6)
811 GAAGAC
BsaHI 1362 GGCGCC G[GA]^CG[CT]C
2034 GGCGCC
BsaI 849 GAGACC GGTCTC(1/5)
1436 GGTCTC
BsmAI 849 GAGAC GTCTC(1/5)
1437 GTCTC
BsmI 1618 GAATGC GAATGC(1/-1)
1786 GAATGC
BspMI 1742 ACCTGC ACCTGC(4/8)
1838 ACCTGC
BsrFI 1561 ACCGGC [GA]^CCGG[CT]
2111 ACCGGC
BstAPI 1622 GCAACGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1874 GCAAGGTGTGC
Cfr10I 1561 ACCGGC [GA]^CCGG[CT]
2111 ACCGGC
EaeI 1644 CGGCCA [CT]^GGCC[GA]
2171 TGGCCG
EcoT14I 142 CCAAGG C^C[AT][AT]GG
2048 CCTTGG
HgaI 1027 GCGTC GACGC(5/10)
1926 GCGTC
Hin1I 1362 GGCGCC G[GA]^CG[CT]C
2034 GGCGCC
KasI 1362 GGCGCC G^GCGCC
2034 GGCGCC
NarI 1362 GGCGCC GG^CGCC
2034 GGCGCC
PpuMI 683 AGGACCT [GA]G^G[AT]CC[CT]
725 AGGACCT
SacI 936 GAGCTC GAGCT^C
1293 GAGCTC
SfoI 1362 GGCGCC GGC^GCC
2034 GGCGCC
SmaI 395 CCCGGG CCC^GGG
1937 CCCGGG
StyI 142 CCAAGG C^C[AT][AT]GG
2048 CCTTGG
XmaCI 395 CCCGGG C^CCGGG
1937 CCCGGG
XmaI 395 CCCGGG C^CCGGG
1937 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII