Restriction map for
pFN21AB6292
(Linear) (Six-base) MAPSORT of: KIBB6292.seq Check: 464 from: 1 to: 2367
KIBB6292.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1751 1847 2367
Size: 1751 96 520
Fragments arranged by size:
1751 520 96
AccI GT'mk_AC
Cuts at: 0 1229 2367
Size: 1229 1138
Acc65I G'GTAC_C
Cuts at: 0 2322 2367
Size: 2322 45
AclI AA'CG_TT
Cuts at: 0 1625 2367
Size: 1625 742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1609 1681 2367
Size: 1609 72 686
Fragments arranged by size:
1609 686 72
AfeI AGC'GCT
Cuts at: 0 1170 2367
Size: 1170 1197
AflIII A'CryG_T
Cuts at: 0 223 1025 1470 2367
Size: 223 802 445 897
Fragments arranged by size:
897 802 445 223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 382 2367
Size: 382 1985
AlwNI CAG_nnn'CTG
Cuts at: 0 46 2367
Size: 46 2321
ApaI G_GGCC'C
Cuts at: 0 801 985 2367
Size: 801 184 1382
Fragments arranged by size:
1382 801 184
ApaLI G'TGCA_C
Cuts at: 0 1678 1906 2367
Size: 1678 228 461
Fragments arranged by size:
1678 461 228
ApoI r'AATT_y
Cuts at: 0 2165 2367
Size: 2165 202
AvaI C'yCGr_G
Cuts at: 0 395 776 1212 1290 1937 2116 2367
Size: 395 381 436 78 647 179 251
Fragments arranged by size:
647 436 395 381 251 179 78
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 104 137 2367
Size: 104 33 2230
Fragments arranged by size:
2230 104 33
BamHI G'GATC_C
Cuts at: 0 2361 2367
Size: 2361 6
BanI G'GyrC_C
Cuts at: 0 1362 2034 2322 2367
Size: 1362 672 288 45
Fragments arranged by size:
1362 672 288 45
BanII G_rGCy'C
Cuts at: 0 757 801 940 985 1054 1297 1317 2367
Size: 757 44 139 45 69 243 20 1050
Fragments arranged by size:
1050 757 243 139 69 45 44 20
BbeI G_GCGC'C
Cuts at: 0 1366 2038 2367
Size: 1366 672 329
Fragments arranged by size:
1366 672 329
BbsI GAAGACnn'nnnn_
Cuts at: 0 750 818 2367
Size: 750 68 1549
Fragments arranged by size:
1549 750 68
BbvCI CC'TCA_GC
Cuts at: 0 1494 2367
Size: 1494 873
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1224 1258 1281 1315 2367
Size: 1224 34 23 34 1052
Fragments arranged by size:
1224 1052 34 34 23
BglI GCCn_nnn'nGGC
Cuts at: 0 1462 2367
Size: 1462 905
BglII A'GATC_T
Cuts at: 0 2055 2277 2367
Size: 2055 222 90
Fragments arranged by size:
2055 222 90
Bme1580I G_kGCm'C
Cuts at: 0 223 801 985 1225 1682 1885 1910 2367
Size: 223 578 184 240 457 203 25 457
Fragments arranged by size:
578 457 457 240 223 203 184 25
BmgBI CAC'GTC
Cuts at: 0 748 2124 2367
Size: 748 1376 243
Fragments arranged by size:
1376 748 243
BmrI ACTGGGnnnn_n'
Cuts at: 0 1893 1956 2367
Size: 1893 63 411
Fragments arranged by size:
1893 411 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 924 1312 1874 2367
Size: 924 388 562 493
Fragments arranged by size:
924 562 493 388
Bpu10I CC'TnA_GC
Cuts at: 0 1165 1494 2367
Size: 1165 329 873
Fragments arranged by size:
1165 873 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 977 1813 1981 2367
Size: 916 61 836 168 386
Fragments arranged by size:
916 836 386 168 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 843 1442 2367
Size: 843 599 925
Fragments arranged by size:
925 843 599
BsaAI yAC'GTr
Cuts at: 0 857 2367
Size: 857 1510
BsaBI GATnn'nnATC
Cuts at: 0 433 2367
Size: 433 1934
BsaHI Gr'CG_yC
Cuts at: 0 1363 2035 2367
Size: 1363 672 332
Fragments arranged by size:
1363 672 332
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 991 1021 1942 1972 2367
Size: 991 30 921 30 395
Fragments arranged by size:
991 921 395 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1231 2233 2238 2367
Size: 1231 1002 5 129
Fragments arranged by size:
1231 1002 129 5
BseYI C'CCAG_C
Cuts at: 0 1513 2302 2367
Size: 1513 789 65
Fragments arranged by size:
1513 789 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1498 1903 1915 2277 2367
Size: 1498 405 12 362 90
Fragments arranged by size:
1498 405 362 90 12
BsiHKAI G_wGCw'C
Cuts at: 0 589 940 1001 1297 1682 1910 1960 2367
Size: 589 351 61 296 385 228 50 407
Fragments arranged by size:
589 407 385 351 296 228 61 50
BsmI GAATG_Cn'
Cuts at: 0 1624 1792 2367
Size: 1624 168 575
Fragments arranged by size:
1624 575 168
Bsp1286I G_dGCh'C
Cuts at: 0 223 589 757 801 940 985 1001 1054
Size: 223 366 168 44 139 45 16 53
Cuts at: 1054 1225 1297 1317 1682 1885 1910 1960 2367
Size: 171 72 20 365 203 25 50 407
Fragments arranged by size:
407 366 365 223 203 171 168 139
72 53 50 45 44 25 20 16
BspHI T'CATG_A
Cuts at: 0 2288 2367
Size: 2288 79
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1751 1847 2367
Size: 1751 96 520
Fragments arranged by size:
1751 520 96
BsrBI CCG'CTC
Cuts at: 0 1944 2367
Size: 1944 423
BsrFI r'CCGG_y
Cuts at: 0 1561 2111 2367
Size: 1561 550 256
Fragments arranged by size:
1561 550 256
BsrGI T'GTAC_A
Cuts at: 0 64 2367
Size: 64 2303
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1628 1880 2367
Size: 1628 252 487
Fragments arranged by size:
1628 487 252
BstYI r'GATC_y
Cuts at: 0 302 2055 2277 2361 2367
Size: 302 1753 222 84 6
Fragments arranged by size:
1753 302 222 84 6
Bsu36I CC'TnA_GG
Cuts at: 0 2250 2367
Size: 2250 117
BtgI C'CryG_G
Cuts at: 0 1442 1460 2174 2367
Size: 1442 18 714 193
Fragments arranged by size:
1442 714 193 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1516 2367
Size: 1516 851
EaeI y'GGCC_r
Cuts at: 0 1644 2171 2367
Size: 1644 527 196
Fragments arranged by size:
1644 527 196
EarI CTCTTCn'nnn_
Cuts at: 0 50 173 344 1213 2367
Size: 50 123 171 869 1154
Fragments arranged by size:
1154 869 171 123 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 142 2367
Size: 142 2225
EcoICRI GAG'CTC
Cuts at: 0 938 1295 2367
Size: 938 357 1072
Fragments arranged by size:
1072 938 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 924 1312 1609 1681 1874 2367
Size: 924 388 297 72 193 493
Fragments arranged by size:
924 493 388 297 193 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 141 2367
Size: 141 2226
EcoO109I rG'GnC_Cy
Cuts at: 0 684 726 798 982 2367
Size: 684 42 72 184 1385
Fragments arranged by size:
1385 684 184 72 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1149 2367
Size: 1149 1218
HaeII r_GCGC'y
Cuts at: 0 1172 1366 2038 2367
Size: 1172 194 672 329
Fragments arranged by size:
1172 672 329 194
HindIII A'AGCT_T
Cuts at: 0 824 2367
Size: 824 1543
KasI G'GCGC_C
Cuts at: 0 1362 2034 2367
Size: 1362 672 333
Fragments arranged by size:
1362 672 333
KpnI G_GTAC'C
Cuts at: 0 2326 2367
Size: 2326 41
MluI A'CGCG_T
Cuts at: 0 1025 2367
Size: 1025 1342
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 2367
Size: 689 1678
MslI CAynn'nnrTG
Cuts at: 0 1785 1869 2140 2367
Size: 1785 84 271 227
Fragments arranged by size:
1785 271 227 84
MspA1I CmG'CkG
Cuts at: 0 1587 2367
Size: 1587 780
NarI GG'CG_CC
Cuts at: 0 1363 2035 2367
Size: 1363 672 332
Fragments arranged by size:
1363 672 332
NspI r_CATG'y
Cuts at: 0 1474 2367
Size: 1474 893
PasI CC'CwG_GG
Cuts at: 0 89 958 1343 2367
Size: 89 869 385 1024
Fragments arranged by size:
1024 869 385 89
PciI A'CATG_T
Cuts at: 0 1470 2367
Size: 1470 897
PfoI T'CCnGG_A
Cuts at: 0 528 2367
Size: 528 1839
PpuMI rG'GwC_Cy
Cuts at: 0 684 726 2367
Size: 684 42 1641
Fragments arranged by size:
1641 684 42
PspOMI G'GGCC_C
Cuts at: 0 797 981 2367
Size: 797 184 1386
Fragments arranged by size:
1386 797 184
PspXI vC'TCGA_Gb
Cuts at: 0 2116 2367
Size: 2116 251
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1015 1047 2367
Size: 1015 32 1320
Fragments arranged by size:
1320 1015 32
PstI C_TGCA'G
Cuts at: 0 1748 2367
Size: 1748 619
SacI G_AGCT'C
Cuts at: 0 940 1297 2367
Size: 940 357 1070
Fragments arranged by size:
1070 940 357
SexAI A'CCwGG_T
Cuts at: 0 1757 2367
Size: 1757 610
SfcI C'TryA_G
Cuts at: 0 1230 1419 1744 1989 2150 2367
Size: 1230 189 325 245 161 217
Fragments arranged by size:
1230 325 245 217 189 161
SfoI GGC'GCC
Cuts at: 0 1364 2036 2367
Size: 1364 672 331
Fragments arranged by size:
1364 672 331
SmaI CCC'GGG
Cuts at: 0 397 1939 2367
Size: 397 1542 428
Fragments arranged by size:
1542 428 397
SmlI C'TyrA_G
Cuts at: 0 931 992 1828 1996 2116 2367
Size: 931 61 836 168 120 251
Fragments arranged by size:
931 836 251 168 120 61
SspI AAT'ATT
Cuts at: 0 292 2367
Size: 292 2075
StuI AGG'CCT
Cuts at: 0 1417 2367
Size: 1417 950
StyI C'CwwG_G
Cuts at: 0 142 2048 2367
Size: 142 1906 319
Fragments arranged by size:
1906 319 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1912 2367
Size: 1912 455
TatI w'GTAC_w
Cuts at: 0 64 632 2367
Size: 64 568 1735
Fragments arranged by size:
1735 568 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 991 1023 1942 1974 2367
Size: 991 32 919 32 393
Fragments arranged by size:
991 919 393 32 32
XhoI C'TCGA_G
Cuts at: 0 2116 2367
Size: 2116 251
XmaI C'CCGG_G
Cuts at: 0 395 1937 2367
Size: 395 1542 430
Fragments arranged by size:
1542 430 395
XmnI GAAnn'nnTTC
Cuts at: 0 512 2367
Size: 512 1855
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlfI
AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrGI BstAPI BstYI Bsu36I
BtgI BtgZI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII HindIII KasI KpnI MluI MmeI
MslI MspA1I NarI NspI PasI PciI PfoI PpuMI
PspOMI PspXI PsrI PstI SacI SexAI SfcI SfoI
SmaI SmlI SspI StuI StyI TaqII TatI TstI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AloI AscI AseI
AsiSI AvrII BciVI BclI BfrBI BlpI BmtI BplI
BsaWI BsiEI BsiWI BsmBI BspEI BsrDI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HpaI MfeI MscI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI
PmlI PpiI PshAI PsiI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XcmI
ZraI