Restriction map for
pFN21AB6268
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
Alw44I Aor51HI ApaI ApaLI Asp718I
AspI AviII BamHI BbeI BbrPI
BcgI BciVI BfrI BglII BsaAI
BsaBI BsgI BsiEI BsiWI BsmBI
BsmI BspLU11I BsrBI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoO65I FseI FspI HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 2023 GACGTC GACGT^C
AccIII 2027 TCCGGA T^CCGGA
AhdI 502 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 500 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 42 GGCGCGCC GG^CGCGCC
AseI 1794 ATTAAT AT^TAAT
AsnI 1794 ATTAAT AT^TAAT
AspEI 502 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2236 CCTAGG C^CTAGG
BbsI 510 GTCTTC GAAGAC(2/6)
BbvCI 1230 CCTCAGC CCTCAGC(-5/-2)
BclI 1758 TGATCA T^GATCA
BglI 2194 GCCCACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2236 CCTAGG C^CTAGG
BlpI 244 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 244 GCTGAGC GC^T[ACGT]AGC
BpuAI 510 GTCTTC GAAGAC(2/6)
BsaWI 2027 TCCGGA [AT]^CCGG[AT]
BseAI 2027 TCCGGA T^CCGGA
Bsp1407I 1750 TGTACA T^GTACA
BspDI 1687 ATCGAT AT^CGAT
BspEI 2027 TCCGGA T^CCGGA
BspHI 444 TCATGA T^CATGA
BsrGI 1750 TGTACA T^GTACA
BssHII 43 GCGCGC G^CGCGC
BstXI 621 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 583 AGATCC [GA]^GATC[CT]
CelII 244 GCTGAGC GC^T[ACGT]AGC
ClaI 1687 ATCGAT AT^CGAT
Eam1105I 502 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1509 GATATC GAT^ATC
EcoT22I 1662 ATGCAT ATGCA^T
FauI 1465 CCCGC CCCGC(4/6)
FbaI 1758 TGATCA T^GATCA
HgaI 1903 GACGC GACGC(5/10)
MflI 583 AGATCC [GA]^GATC[CT]
MroI 2027 TCCGGA T^CCGGA
NsiI 1662 ATGCAT ATGCA^T
PflMI 21 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 197 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1794 ATTAAT AT^TAAT
RcaI 444 TCATGA T^CATGA
SacI 412 GAGCTC GAGCT^C
SalI 571 GTCGAC G^TCGAC
SapI 270 GAAGAGC GCTCTTC(1/4)
SspBI 1750 TGTACA T^GTACA
SspI 83 AATATT AAT^ATT
StuI 785 AGGCCT AGG^CCT
Van91I 21 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 194 CCAGGGACCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 583 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 118 GTAGAC GT^[AC][GT]AC
571 GTCGAC
AcsI 1195 GAATTC [GA]^AATT[CT]
1590 GAATTC
AcyI 1903 GACGCC G[GA]^CG[CT]C
2023 GACGTC
AocI 851 CCTCAGG CC^T[ACGT]AGG
2155 CCTGAGG
ApoI 1195 GAATTC [GA]^AATT[CT]
1590 GAATTC
Asp700I 1586 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2298 GAACAGATTC
AspHI 412 GAGCTC G[AT]GC[AT]^C
730 GAGCAC
BanI 1425 GGTGCC G^G[CT][GA]CC
2202 GGCACC
BcnI 60 CCCGG CC^[GC]GG
1247 CCCGG
BsaHI 1903 GACGCC G[GA]^CG[CT]C
2023 GACGTC
BsiHKAI 412 GAGCTC G[AT]GC[AT]^C
730 GAGCAC
BspMI 1889 ACCTGC ACCTGC(4/8)
1602 GCAGGT
BsrDI 644 GCAATG GCAATG(2/0)
1659 GCAATG
BsrFI 1392 GCCGGC [GA]^CCGG[CT]
2163 GCCGGC
BssSI 172 CACGAG CACGAG(-5/-1)
2018 CTCGTG
Bsu36I 851 CCTCAGG CC^T[ACGT]AGG
2155 CCTGAGG
Cfr10I 1392 GCCGGC [GA]^CCGG[CT]
2163 GCCGGC
DraII 197 GGGACCT [GA]G^G[ACGT]CC[CT]
818 AGGCCCT
DraIII 427 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2015 CACCTCGTG
Eco81I 851 CCTCAGG CC^T[ACGT]AGG
2155 CCTGAGG
EcoO109I 197 GGGACCT [GA]G^G[ACGT]CC[CT]
818 AGGCCCT
EcoRI 1195 GAATTC G^AATTC
1590 GAATTC
HaeII 15 GGCGCT [GA]GCGC^[CT]
287 AGCGCC
Hin1I 1903 GACGCC G[GA]^CG[CT]C
2023 GACGTC
HincII 571 GTCGAC GT[CT]^[GA]AC
1996 GTCAAC
HindII 571 GTCGAC GT[CT]^[GA]AC
1996 GTCAAC
HindIII 186 AAGCTT A^AGCTT
2288 AAGCTT
MaeII 569 ACGT A^CGT
2024 ACGT
NaeI 1392 GCCGGC GCC^GGC
2163 GCCGGC
NciI 60 CCCGG CC^[GC]GG
1247 CCCGG
NcoI 699 CCATGG C^CATGG
2105 CCATGG
NgoMIV 1392 GCCGGC G^CCGGC
2163 GCCGGC
NspI 177 GCATGT [GA]CATG^[CT]
1953 ACATGC
PaeR7I 415 CTCGAG C^TCGAG
1501 CTCGAG
PstI 37 CTGCAG CTGCA^G
304 CTGCAG
PvuII 1762 CAGCTG CAG^CTG
2063 CAGCTG
ScaI 1671 AGTACT AGT^ACT
1926 AGTACT
SmlI 415 CTCGAG C^T[CT][GA]AG
1501 CTCGAG
XhoI 415 CTCGAG C^TCGAG
1501 CTCGAG
XmnI 1586 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2298 GAACAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BalI BanII BbvI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoNI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I