(Circular) (Six-base) MAPSORT of: pFN21AB6268.seq Check: 2286 from: 1 to: 7021 pFN21AB6268 7021 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3598 3598 Size: 7021 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4032 4831 278 Size: 53 83 186 936 2496 799 2468 Fragments arranged by size: 2496 2468 936 799 186 83 53 AccI GT'mk_AC Cuts at: 1937 2124 2577 4364 1937 Size: 187 453 1787 4594 Fragments arranged by size: 4594 1787 453 187 Acc65I G'GTAC_C Cuts at: 4342 4342 Size: 7021 AclI AA'CG_TT Cuts at: 1563 5148 5521 1563 Size: 3585 373 3063 Fragments arranged by size: 3585 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2487 2949 2951 3267 3887 5084 6468 6736 Size: 855 462 2 316 620 1197 1384 268 Cuts at: 6736 1632 Size: 1917 Fragments arranged by size: 1917 1384 1197 855 620 462 316 268 2 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6824 AflIII A'CryG_T Cuts at: 1203 5920 6897 1203 Size: 4717 977 1327 Fragments arranged by size: 4717 1327 977 AgeI A'CCGG_T Cuts at: 5852 5852 Size: 7021 AhdI GACnn_n'nnGTC Cuts at: 2512 5750 2512 Size: 3238 3783 AleI CACnn'nnGTG Cuts at: 1166 2694 1166 Size: 1528 5493 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7021 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4295 4327 4295 Size: 32 6989 AlwNI CAG_nnn'CTG Cuts at: 2510 4764 6336 2510 Size: 2254 1572 3195 Fragments arranged by size: 3195 2254 1572 ApaLI G'TGCA_C Cuts at: 5078 6234 5078 Size: 1156 5865 ApoI r'AATT_y Cuts at: 1493 3200 3595 4330 4556 4708 6817 1493 Size: 1707 395 735 226 152 2109 1697 Fragments arranged by size: 2109 1707 1697 735 395 226 152 AscI GG'CGCG_CC Cuts at: 2048 2048 Size: 7021 AseI AT'TA_AT Cuts at: 160 1032 3800 5575 160 Size: 872 2768 1775 1606 Fragments arranged by size: 2768 1775 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7021 AvaI C'yCGr_G Cuts at: 1870 1943 2298 2420 2669 3506 3912 4346 1870 Size: 73 355 122 249 837 406 434 4545 Fragments arranged by size: 4545 837 434 406 355 249 122 73 AvrII C'CTAG_G Cuts at: 4241 4241 Size: 7021 BamHI G'GATC_C Cuts at: 4351 4718 4351 Size: 367 6654 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3430 4207 4342 5797 618 Size: 325 217 627 1643 777 135 1455 1842 Fragments arranged by size: 1842 1643 1455 777 627 325 217 135 BanII G_rGCy'C Cuts at: 1391 2421 2613 3164 4340 1391 Size: 1030 192 551 1176 4072 Fragments arranged by size: 4072 1176 1030 551 192 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2508 928 Size: 971 609 5441 Fragments arranged by size: 5441 971 609 BbvCI CC'TCA_GC Cuts at: 3236 3236 Size: 7021 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5212 5246 1903 Size: 34 3275 34 3678 Fragments arranged by size: 3678 3275 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4913 6123 4913 Size: 1210 5811 BclI T'GATC_A Cuts at: 1548 3763 1548 Size: 2215 4806 BfrBI ATG'CAT Cuts at: 3669 3669 Size: 7021 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4205 5632 Size: 107 122 71 631 605 337 2196 1427 Cuts at: 5632 136 Size: 1525 Fragments arranged by size: 2196 1525 1427 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7016 7016 Size: 7021 BlpI GC'TnA_GC Cuts at: 2250 4438 2250 Size: 2188 4833 Bme1580I G_kGCm'C Cuts at: 1790 4201 5082 6238 1790 Size: 2411 881 1156 2573 Fragments arranged by size: 2573 2411 1156 881 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7021 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2151 2209 3167 Size: 416 53 83 399 24 734 58 958 Cuts at: 3167 3591 4752 5710 442 Size: 424 1161 958 1753 Fragments arranged by size: 1753 1161 958 958 734 424 416 399 83 58 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7021 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2809 2809 Size: 7021 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2615 3102 3423 5681 1239 Size: 1376 487 321 2258 2579 Fragments arranged by size: 2579 2258 1376 487 321 Bpu10I CC'TnA_GC Cuts at: 3236 3236 Size: 7021 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5146 6011 6309 5146 Size: 865 298 5858 Fragments arranged by size: 5858 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2419 2991 3063 5684 882 Size: 617 920 572 72 2621 2219 Fragments arranged by size: 2621 2219 920 617 572 72 BsaAI yAC'GTr Cuts at: 493 1204 4812 493 Size: 711 3608 2702 Fragments arranged by size: 3608 2702 711 BsaBI GATnn'nnATC Cuts at: 7015 7015 Size: 7021 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3909 4029 4828 5210 Size: 53 83 186 936 2376 120 799 382 Cuts at: 5210 5845 275 Size: 635 1451 Fragments arranged by size: 2376 1451 936 799 635 382 186 120 83 53 BsaWI w'CCGG_w Cuts at: 4032 5454 5852 6126 6273 6684 4032 Size: 1422 398 274 147 411 4369 Fragments arranged by size: 4369 1422 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4056 4086 4295 4325 1178 Size: 30 165 30 2653 30 209 30 3874 Fragments arranged by size: 3874 2653 209 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 2336 2470 2612 2852 3194 3729 4051 Size: 1147 3 134 142 240 342 535 322 Cuts at: 4051 4077 4178 1186 Size: 26 101 4029 Fragments arranged by size: 4029 1147 535 342 322 240 142 134 101 26 3 BseYI C'CCAG_C Cuts at: 4154 6224 6720 4154 Size: 2070 496 4455 Fragments arranged by size: 4455 2070 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6448 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4471 5232 6260 1540 Size: 275 186 2470 761 1028 2301 Fragments arranged by size: 2470 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2421 2739 5082 5167 6238 2421 Size: 318 2343 85 1071 3204 Fragments arranged by size: 3204 2343 1071 318 85 BsmI GAATG_Cn' Cuts at: 4532 4625 4532 Size: 93 6928 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2421 2613 2739 3164 4201 4340 5082 Size: 399 631 192 126 425 1037 139 742 Cuts at: 5082 5167 6238 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1037 742 631 425 399 192 139 126 85 BspEI T'CCGG_A Cuts at: 4032 6684 4032 Size: 2652 4369 BspHI T'CATG_A Cuts at: 2449 4910 6640 2449 Size: 2461 1730 2830 Fragments arranged by size: 2830 2461 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3598 3903 4376 844 Size: 2754 305 473 3489 Fragments arranged by size: 3489 2754 473 305 BsrBI CCG'CTC Cuts at: 1394 1823 4908 1394 Size: 429 3085 3507 Fragments arranged by size: 3507 3085 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2656 3671 5516 5690 65 Size: 1183 1408 1015 1845 174 1396 Fragments arranged by size: 1845 1408 1396 1183 1015 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3397 4168 5665 5852 1700 Size: 110 1587 771 1497 187 2869 Fragments arranged by size: 2869 1587 1497 771 187 110 BsrGI T'GTAC_A Cuts at: 96 3755 96 Size: 3659 3362 BssHII G'CGCG_C Cuts at: 1925 2048 1925 Size: 123 6898 BssSI C'ACGA_G Cuts at: 2177 4023 5081 6093 2177 Size: 1846 1058 1012 3105 Fragments arranged by size: 3105 1846 1058 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7021 BstBI TT'CG_AA Cuts at: 5825 5825 Size: 7021 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7021 BstXI CCAn_nnnn'nTGG Cuts at: 2633 2633 Size: 7021 BstYI r'GATC_y Cuts at: 1972 2588 4351 4718 5103 5120 6572 6658 6670 Size: 616 1763 367 385 17 1452 86 12 Cuts at: 6670 7016 1972 Size: 346 1977 Fragments arranged by size: 1977 1763 1452 616 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2857 4161 2857 Size: 1304 5717 BtgI C'CryG_G Cuts at: 513 1065 2004 2358 2704 2766 3288 4110 513 Size: 552 939 354 346 62 522 822 3424 Fragments arranged by size: 3424 939 822 552 522 354 346 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6282 Fragments arranged by size: 6282 683 56 BtsI GCAGTG_nn' Cuts at: 3494 4201 4537 5331 5351 3494 Size: 707 336 794 20 5164 Fragments arranged by size: 5164 794 707 336 20 ClaI AT'CG_AT Cuts at: 1554 3693 1554 Size: 2139 4882 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3277 3312 529 Size: 35 2713 35 4238 Fragments arranged by size: 4238 2713 35 35 DraI TTT'AAA Cuts at: 4325 4325 Size: 7021 DraIII CAC_nnn'GTG Cuts at: 2437 4025 2437 Size: 1588 5433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6028 809 Size: 507 4712 1802 Fragments arranged by size: 4712 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2527 4011 Size: 54 1425 50 275 18 225 472 1484 Cuts at: 4011 4278 4468 5357 6986 8 Size: 267 190 889 1629 43 Fragments arranged by size: 1629 1484 1425 889 472 275 267 225 190 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4468 1537 Size: 275 2656 4090 Fragments arranged by size: 4090 2656 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2270 2506 3553 3861 4951 1353 Size: 917 236 1047 308 1090 3423 Fragments arranged by size: 3423 1090 1047 917 308 236 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 4120 5608 5982 6128 387 Size: 1064 138 438 2093 1488 374 146 1280 Fragments arranged by size: 2093 1488 1280 1064 438 374 146 138 EcoICRI GAG'CTC Cuts at: 2419 2419 Size: 7021 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2487 2615 2949 2951 3102 3267 3423 Size: 393 855 128 334 2 151 165 156 Cuts at: 3423 3887 5084 5681 6468 6736 1239 Size: 464 1197 597 787 268 1524 Fragments arranged by size: 1524 1197 855 787 597 464 393 334 268 165 156 151 128 2 EcoNI CCTnn'n_nnAGG Cuts at: 2312 2597 2637 2312 Size: 285 40 6696 Fragments arranged by size: 6696 285 40 EcoO109I rG'GnC_Cy Cuts at: 2203 2824 2203 Size: 621 6400 EcoRI G'AATT_C Cuts at: 3200 3595 4330 4708 3200 Size: 395 735 378 5513 Fragments arranged by size: 5513 735 395 378 EcoRV GAT'ATC Cuts at: 3516 3516 Size: 7021 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4012 4012 Size: 7021 FspI TGC'GCA Cuts at: 5527 5527 Size: 7021 HaeII r_GCGC'y Cuts at: 1678 2024 2296 6168 1678 Size: 346 272 3872 2531 Fragments arranged by size: 3872 2531 346 272 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2498 2530 2726 2758 3137 Size: 32 135 32 926 32 196 32 379 Cuts at: 3137 3169 3500 3532 3860 3892 4056 4088 5662 Size: 32 331 32 328 32 164 32 1574 Cuts at: 5662 5694 5736 5768 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1574 926 379 331 328 196 164 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2578 4003 4365 4611 669 Size: 988 281 640 1425 362 246 3079 Fragments arranged by size: 3079 1425 988 640 362 281 246 HindIII A'AGCT_T Cuts at: 748 2191 4293 748 Size: 1443 2102 3476 Fragments arranged by size: 3476 2102 1443 HpaI GTT'AAC Cuts at: 4611 4611 Size: 7021 KpnI G_GTAC'C Cuts at: 4346 4346 Size: 7021 MfeI C'AATT_G Cuts at: 4620 4620 Size: 7021 MluI A'CGCG_T Cuts at: 6897 6897 Size: 7021 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2167 6135 6319 1305 Size: 862 3968 184 2007 Fragments arranged by size: 3968 2007 862 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2529 4013 4280 10 Size: 54 1425 343 697 1484 267 2751 Fragments arranged by size: 2751 1484 1425 697 343 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 2181 2448 2649 2694 4979 5338 5497 Size: 648 1015 267 201 45 2285 359 159 Cuts at: 5497 518 Size: 2042 Fragments arranged by size: 2285 2042 1015 648 359 267 201 159 45 MspA1I CmG'CkG Cuts at: 1606 3769 3922 4070 4437 5114 6262 6507 1606 Size: 2163 153 148 367 677 1148 245 2120 Fragments arranged by size: 2163 2120 1148 677 367 245 153 148 NaeI GCC'GGC Cuts at: 3399 4170 3399 Size: 771 6250 NcoI C'CATG_G Cuts at: 513 1065 2004 2704 4110 513 Size: 552 939 700 1406 3424 Fragments arranged by size: 3424 1406 939 700 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7021 NgoMIV G'CCGG_C Cuts at: 3397 4168 3397 Size: 771 6250 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7021 NotI GC'GGCC_GC Cuts at: 4468 4468 Size: 7021 NsiI A_TGCA'T Cuts at: 3671 3671 Size: 7021 NspI r_CATG'y Cuts at: 1132 1229 2186 3962 4379 5924 1132 Size: 97 957 1776 417 1545 2229 Fragments arranged by size: 2229 1776 1545 957 417 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7021 PciI A'CATG_T Cuts at: 5920 5920 Size: 7021 PflMI CCAn_nnn'nTGG Cuts at: 1108 2032 1108 Size: 924 6097 PmeI GTTT'AAAC Cuts at: 4325 4325 Size: 7021 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4295 4327 5090 5122 6603 6635 4295 Size: 32 763 32 1481 32 4681 Fragments arranged by size: 4681 1481 763 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2203 2203 Size: 7021 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7021 PsiI TTA'TAA Cuts at: 4591 4591 Size: 7021 PspXI vC'TCGA_Gb Cuts at: 3506 3506 Size: 7021 PstI C_TGCA'G Cuts at: 830 2046 2313 4373 830 Size: 1216 267 2060 3478 Fragments arranged by size: 3478 2060 1216 267 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7021 PvuII CAG'CTG Cuts at: 3769 4070 3769 Size: 301 6720 SacI G_AGCT'C Cuts at: 2421 2421 Size: 7021 SalI G'TCGA_C Cuts at: 1936 2576 4363 1936 Size: 640 1787 4594 Fragments arranged by size: 4594 1787 640 SapI GCTCTTCn'nnn_ Cuts at: 2270 2270 Size: 7021 SbfI CC_TGCA'GG Cuts at: 4373 4373 Size: 7021 ScaI AGT'ACT Cuts at: 3678 3933 5269 6981 3678 Size: 255 1336 1712 3718 Fragments arranged by size: 3718 1712 1336 255 SfcI C'TryA_G Cuts at: 826 1045 2042 2309 2374 2584 4369 5504 6185 Size: 219 997 267 65 210 1785 1135 681 Cuts at: 6185 6376 826 Size: 191 1471 Fragments arranged by size: 1785 1471 1135 997 681 267 219 210 191 65 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7021 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7021 SmaI CCC'GGG Cuts at: 1872 4348 1872 Size: 2476 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2420 3506 5125 6026 6288 820 Size: 197 926 477 1086 1619 901 262 1553 Fragments arranged by size: 1619 1553 1086 926 901 477 262 197 SnaBI TAC'GTA Cuts at: 493 4812 493 Size: 4319 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7021 SphI G_CATG'C Cuts at: 1132 4379 1132 Size: 3247 3774 SspI AAT'ATT Cuts at: 5 52 2090 4945 5 Size: 47 2038 2855 2081 Fragments arranged by size: 2855 2081 2038 47 StuI AGG'CCT Cuts at: 1517 2792 1517 Size: 1275 5746 StyI C'CwwG_G Cuts at: 513 1065 2004 2704 3081 3369 4110 4241 4460 Size: 552 939 700 377 288 741 131 219 Cuts at: 4460 513 Size: 3074 Fragments arranged by size: 3074 939 741 700 552 377 288 219 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2684 5048 5065 5218 5403 5848 325 Size: 1255 1104 2364 17 153 185 445 1498 Fragments arranged by size: 2364 1498 1255 1104 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3676 3755 3931 Size: 274 80 33 51 1443 1699 79 176 Cuts at: 3931 5267 6979 96 Size: 1336 1712 138 Fragments arranged by size: 1712 1699 1443 1336 274 176 138 80 79 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2573 2605 2729 2761 6981 7013 2573 Size: 32 124 32 4220 32 2581 Fragments arranged by size: 4220 2581 124 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7021 XbaI T'CTAG_A Cuts at: 4357 4357 Size: 7021 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2206 2206 Size: 7021 XhoI C'TCGA_G Cuts at: 1943 2420 3506 1943 Size: 477 1086 5458 Fragments arranged by size: 5458 1086 477 XmaI C'CCGG_G Cuts at: 1870 4346 1870 Size: 2476 4545 XmnI GAAnn'nnTTC Cuts at: 3595 4307 5150 3595 Size: 712 843 5466 Fragments arranged by size: 5466 843 712 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4030 4829 276 Size: 53 83 186 936 2496 799 2468 Fragments arranged by size: 2496 2468 936 799 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI BaeI BbeI BsiWI BsmBI BstZ17I FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI