Restriction map for
pFN21AB6268
(Linear) (Six-base) MAPSORT of: KIBB6268.seq Check: 6705 from: 1 to: 2316
KIBB6268.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1593 2316
Size: 1593 723
AatII G_ACGT'C
Cuts at: 0 2027 2316
Size: 2027 289
AccI GT'mk_AC
Cuts at: 0 119 572 2316
Size: 119 453 1744
Fragments arranged by size:
1744 453 119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 944 946 1262 1882 2316
Size: 482 462 2 316 620 434
Fragments arranged by size:
620 482 462 434 316 2
AhdI GACnn_n'nnGTC
Cuts at: 0 507 2316
Size: 507 1809
AleI CACnn'nnGTG
Cuts at: 0 689 2316
Size: 689 1627
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2290 2316
Size: 2290 26
AlwNI CAG_nnn'CTG
Cuts at: 0 505 2316
Size: 505 1811
ApoI r'AATT_y
Cuts at: 0 1195 1590 2316
Size: 1195 395 726
Fragments arranged by size:
1195 726 395
AscI GG'CGCG_CC
Cuts at: 0 43 2316
Size: 43 2273
AseI AT'TA_AT
Cuts at: 0 1795 2316
Size: 1795 521
AvaI C'yCGr_G
Cuts at: 0 293 415 664 1501 1907 2316
Size: 293 122 249 837 406 409
Fragments arranged by size:
837 409 406 293 249 122
AvrII C'CTAG_G
Cuts at: 0 2236 2316
Size: 2236 80
BanI G'GyrC_C
Cuts at: 0 1425 2202 2316
Size: 1425 777 114
Fragments arranged by size:
1425 777 114
BanII G_rGCy'C
Cuts at: 0 416 608 1159 2316
Size: 416 192 551 1157
Fragments arranged by size:
1157 551 416 192
BbsI GAAGACnn'nnnn_
Cuts at: 0 503 2316
Size: 503 1813
BbvCI CC'TCA_GC
Cuts at: 0 1231 2316
Size: 1231 1085
BclI T'GATC_A
Cuts at: 0 1758 2316
Size: 1758 558
BfrBI ATG'CAT
Cuts at: 0 1664 2316
Size: 1664 652
BglI GCCn_nnn'nGGC
Cuts at: 0 2200 2316
Size: 2200 116
BlpI GC'TnA_GC
Cuts at: 0 245 2316
Size: 245 2071
Bme1580I G_kGCm'C
Cuts at: 0 2196 2316
Size: 2196 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 146 204 1162 1586 2316
Size: 146 58 958 424 730
Fragments arranged by size:
958 730 424 146 58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 804 2316
Size: 804 1512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 1097 1418 2316
Size: 610 487 321 898
Fragments arranged by size:
898 610 487 321
Bpu10I CC'TnA_GC
Cuts at: 0 1231 2316
Size: 1231 1085
BsaI GGTCTCn'nnnn_
Cuts at: 0 414 986 1058 2316
Size: 414 572 72 1258
Fragments arranged by size:
1258 572 414 72
BsaHI Gr'CG_yC
Cuts at: 0 1904 2024 2316
Size: 1904 120 292
Fragments arranged by size:
1904 292 120
BsaWI w'CCGG_w
Cuts at: 0 2027 2316
Size: 2027 289
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2051 2081 2290 2316
Size: 2051 30 209 26
Fragments arranged by size:
2051 209 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 331 465 607 847 1189 1724 2046
Size: 328 3 134 142 240 342 535 322
Cuts at: 2046 2072 2173 2316
Size: 26 101 143
Fragments arranged by size:
535 342 328 322 240 143 142 134
101 26 3
BseYI C'CCAG_C
Cuts at: 0 2149 2316
Size: 2149 167
BsiHKAI G_wGCw'C
Cuts at: 0 416 734 2316
Size: 416 318 1582
Fragments arranged by size:
1582 416 318
Bsp1286I G_dGCh'C
Cuts at: 0 416 608 734 1159 2196 2316
Size: 416 192 126 425 1037 120
Fragments arranged by size:
1037 425 416 192 126 120
BspEI T'CCGG_A
Cuts at: 0 2027 2316
Size: 2027 289
BspHI T'CATG_A
Cuts at: 0 444 2316
Size: 444 1872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1593 1898 2316
Size: 1593 305 418
Fragments arranged by size:
1593 418 305
BsrDI GCAATG_nn'
Cuts at: 0 651 1666 2316
Size: 651 1015 650
Fragments arranged by size:
1015 651 650
BsrFI r'CCGG_y
Cuts at: 0 1392 2163 2316
Size: 1392 771 153
Fragments arranged by size:
1392 771 153
BsrGI T'GTAC_A
Cuts at: 0 1750 2316
Size: 1750 566
BssHII G'CGCG_C
Cuts at: 0 43 2316
Size: 43 2273
BssSI C'ACGA_G
Cuts at: 0 172 2018 2316
Size: 172 1846 298
Fragments arranged by size:
1846 298 172
BstXI CCAn_nnnn'nTGG
Cuts at: 0 628 2316
Size: 628 1688
BstYI r'GATC_y
Cuts at: 0 583 2316
Size: 583 1733
Bsu36I CC'TnA_GG
Cuts at: 0 852 2156 2316
Size: 852 1304 160
Fragments arranged by size:
1304 852 160
BtgI C'CryG_G
Cuts at: 0 353 699 761 1283 2105 2316
Size: 353 346 62 522 822 211
Fragments arranged by size:
822 522 353 346 211 62
BtsI GCAGTG_nn'
Cuts at: 0 1489 2196 2316
Size: 1489 707 120
Fragments arranged by size:
1489 707 120
ClaI AT'CG_AT
Cuts at: 0 1688 2316
Size: 1688 628
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1272 1307 2316
Size: 1272 35 1009
Fragments arranged by size:
1272 1009 35
DraIII CAC_nnn'GTG
Cuts at: 0 432 2020 2316
Size: 432 1588 296
Fragments arranged by size:
1588 432 296
EaeI y'GGCC_r
Cuts at: 0 50 522 2006 2273 2316
Size: 50 472 1484 267 43
Fragments arranged by size:
1484 472 267 50 43
EarI CTCTTCn'nnn_
Cuts at: 0 265 501 1548 1856 2316
Size: 265 236 1047 308 460
Fragments arranged by size:
1047 460 308 265 236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 2115 2316
Size: 22 2093 201
Fragments arranged by size:
2093 201 22
EcoICRI GAG'CTC
Cuts at: 0 414 2316
Size: 414 1902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 610 944 946 1097 1262 1418 1882
Size: 482 128 334 2 151 165 156 464
Cuts at: 1882 2316
Size: 434
Fragments arranged by size:
482 464 434 334 165 156 151 128
2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 307 592 632 2316
Size: 307 285 40 1684
Fragments arranged by size:
1684 307 285 40
EcoO109I rG'GnC_Cy
Cuts at: 0 198 819 2316
Size: 198 621 1497
Fragments arranged by size:
1497 621 198
EcoRI G'AATT_C
Cuts at: 0 1195 1590 2316
Size: 1195 395 726
Fragments arranged by size:
1195 726 395
EcoRV GAT'ATC
Cuts at: 0 1511 2316
Size: 1511 805
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2007 2316
Size: 2007 309
HaeII r_GCGC'y
Cuts at: 0 19 291 2316
Size: 19 272 2025
Fragments arranged by size:
2025 272 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 721 753 1132 1164 1495 1527
Size: 493 32 196 32 379 32 331 32
Cuts at: 1527 1855 1887 2051 2083 2316
Size: 328 32 164 32 233
Fragments arranged by size:
493 379 331 328 233 196 164 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 573 1998 2316
Size: 573 1425 318
Fragments arranged by size:
1425 573 318
HindIII A'AGCT_T
Cuts at: 0 186 2288 2316
Size: 186 2102 28
Fragments arranged by size:
2102 186 28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 2316
Size: 162 2154
MscI TGG'CCA
Cuts at: 0 524 2008 2275 2316
Size: 524 1484 267 41
Fragments arranged by size:
1484 524 267 41
MslI CAynn'nnrTG
Cuts at: 0 176 443 644 689 2316
Size: 176 267 201 45 1627
Fragments arranged by size:
1627 267 201 176 45
MspA1I CmG'CkG
Cuts at: 0 1764 1917 2065 2316
Size: 1764 153 148 251
Fragments arranged by size:
1764 251 153 148
NaeI GCC'GGC
Cuts at: 0 1394 2165 2316
Size: 1394 771 151
Fragments arranged by size:
1394 771 151
NcoI C'CATG_G
Cuts at: 0 699 2105 2316
Size: 699 1406 211
Fragments arranged by size:
1406 699 211
NgoMIV G'CCGG_C
Cuts at: 0 1392 2163 2316
Size: 1392 771 153
Fragments arranged by size:
1392 771 153
NsiI A_TGCA'T
Cuts at: 0 1666 2316
Size: 1666 650
NspI r_CATG'y
Cuts at: 0 181 1957 2316
Size: 181 1776 359
Fragments arranged by size:
1776 359 181
PflMI CCAn_nnn'nTGG
Cuts at: 0 27 2316
Size: 27 2289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2290 2316
Size: 2290 26
PpuMI rG'GwC_Cy
Cuts at: 0 198 2316
Size: 198 2118
PspXI vC'TCGA_Gb
Cuts at: 0 1501 2316
Size: 1501 815
PstI C_TGCA'G
Cuts at: 0 41 308 2316
Size: 41 267 2008
Fragments arranged by size:
2008 267 41
PvuII CAG'CTG
Cuts at: 0 1764 2065 2316
Size: 1764 301 251
Fragments arranged by size:
1764 301 251
SacI G_AGCT'C
Cuts at: 0 416 2316
Size: 416 1900
SalI G'TCGA_C
Cuts at: 0 571 2316
Size: 571 1745
SapI GCTCTTCn'nnn_
Cuts at: 0 265 2316
Size: 265 2051
ScaI AGT'ACT
Cuts at: 0 1673 1928 2316
Size: 1673 255 388
Fragments arranged by size:
1673 388 255
SfcI C'TryA_G
Cuts at: 0 37 304 369 579 2316
Size: 37 267 65 210 1737
Fragments arranged by size:
1737 267 210 65 37
SmlI C'TyrA_G
Cuts at: 0 415 1501 2316
Size: 415 1086 815
Fragments arranged by size:
1086 815 415
SspI AAT'ATT
Cuts at: 0 85 2316
Size: 85 2231
StuI AGG'CCT
Cuts at: 0 787 2316
Size: 787 1529
StyI C'CwwG_G
Cuts at: 0 699 1076 1364 2105 2236 2316
Size: 699 377 288 741 131 80
Fragments arranged by size:
741 699 377 288 131 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 679 2316
Size: 679 1637
TatI w'GTAC_w
Cuts at: 0 1671 1750 1926 2316
Size: 1671 79 176 390
Fragments arranged by size:
1671 390 176 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 568 600 724 756 2316
Size: 568 32 124 32 1560
Fragments arranged by size:
1560 568 124 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 201 2316
Size: 201 2115
XhoI C'TCGA_G
Cuts at: 0 415 1501 2316
Size: 415 1086 815
Fragments arranged by size:
1086 815 415
XmnI GAAnn'nnTTC
Cuts at: 0 1590 2302 2316
Size: 1590 712 14
Fragments arranged by size:
1590 712 14
ZraI GAC'GTC
Cuts at: 0 2025 2316
Size: 2025 291
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AleI AloI AlwNI
ApoI AscI AseI AvaI AvrII BanI BanII BbsI
BbvCI BclI BfrBI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstXI BstYI Bsu36I BtgI BtsI ClaI
CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
NspI PflMI PpiI PpuMI PspXI PstI PvuII SacI
SalI SapI ScaI SfcI SmlI SspI StuI StyI
TaqII TatI TstI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AlfI ApaI
ApaLI AsiSI BaeI BamHI BbeI BcgI BciVI BglII
BmgBI BmtI BpuEI BsaAI BsaBI BsgI BsiEI BsiWI
BsmI BsmBI BsrBI BstAPI BstBI BstEII BstZ17I BtgZI
DraI DrdI EagI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PshAI PsiI
PspOMI PsrI PvuI RsrII SacII SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XmaI