(Circular) (Six-base) MAPSORT of: pFN21AB6264.seq Check: 9153 from: 1 to: 7012 pFN21AB6264 7012 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2199 2199 Size: 7012 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4822 278 Size: 53 83 186 936 3286 2468 Fragments arranged by size: 3286 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2695 4355 1937 Size: 758 1660 4594 Fragments arranged by size: 4594 1660 758 Acc65I G'GTAC_C Cuts at: 4333 4333 Size: 7012 AclI AA'CG_TT Cuts at: 1563 5139 5512 1563 Size: 3576 373 3063 Fragments arranged by size: 3576 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2074 2238 2593 2927 2992 5075 6459 6727 Size: 442 164 355 334 65 2083 1384 268 Cuts at: 6727 1632 Size: 1917 Fragments arranged by size: 2083 1917 1384 442 355 334 268 164 65 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6815 AflIII A'CryG_T Cuts at: 1203 2956 4158 5911 6888 1203 Size: 1753 1202 1753 977 1327 Fragments arranged by size: 1753 1753 1327 1202 977 AgeI A'CCGG_T Cuts at: 3435 5843 3435 Size: 2408 4604 AhdI GACnn_n'nnGTC Cuts at: 5741 5741 Size: 7012 AleI CACnn'nnGTG Cuts at: 1166 2969 3666 1166 Size: 1803 697 4512 Fragments arranged by size: 4512 1803 697 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7012 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2196 2228 2196 Size: 32 6980 AlwNI CAG_nnn'CTG Cuts at: 2910 3162 3834 3868 4086 4755 6327 2910 Size: 252 672 34 218 669 1572 3595 Fragments arranged by size: 3595 1572 672 669 252 218 34 ApaLI G'TGCA_C Cuts at: 5069 6225 5069 Size: 1156 5856 ApoI r'AATT_y Cuts at: 1493 2405 2519 2622 2706 2874 3126 3210 3378 Size: 912 114 103 84 168 252 84 168 Cuts at: 3378 3630 3714 3798 4050 4134 4321 4547 4699 Size: 252 84 84 252 84 187 226 152 Cuts at: 4699 6808 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 912 252 252 252 226 187 168 168 152 114 103 84 84 84 84 84 AseI AT'TA_AT Cuts at: 160 1032 5566 160 Size: 872 4534 1606 Fragments arranged by size: 4534 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7012 AvaI C'yCGr_G Cuts at: 1870 1943 4337 1870 Size: 73 2394 4545 Fragments arranged by size: 4545 2394 73 BamHI G'GATC_C Cuts at: 4342 4709 4342 Size: 367 6645 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2130 4333 5788 618 Size: 325 217 627 343 2203 1455 1842 Fragments arranged by size: 2203 1842 1455 627 343 325 217 BanII G_rGCy'C Cuts at: 1391 4331 1391 Size: 2940 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2855 2998 4016 928 Size: 971 956 143 1018 3924 Fragments arranged by size: 3924 1018 971 956 143 BbvCI CC'TCA_GC Cuts at: 2038 2038 Size: 7012 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5203 5237 1903 Size: 34 50 34 3182 34 3678 Fragments arranged by size: 3678 3182 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2362 2385 4904 6114 2362 Size: 23 2519 1210 3260 Fragments arranged by size: 3260 2519 1210 23 BclI T'GATC_A Cuts at: 1548 1548 Size: 7012 BfrBI ATG'CAT Cuts at: 2484 2782 3240 2484 Size: 298 458 6256 Fragments arranged by size: 6256 458 298 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5623 136 Size: 107 122 71 631 605 3951 1525 Fragments arranged by size: 3951 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2686 7007 2686 Size: 4321 2691 BlpI GC'TnA_GC Cuts at: 4429 4429 Size: 7012 Bme1580I G_kGCm'C Cuts at: 1790 2263 5073 6229 1790 Size: 473 2810 1156 2573 Fragments arranged by size: 2810 2573 1156 473 BmgBI CAC'GTC Cuts at: 1318 2282 1318 Size: 964 6048 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3564 4743 5701 Size: 416 53 83 399 24 2147 1179 958 Cuts at: 5701 442 Size: 1753 Fragments arranged by size: 2147 1753 1179 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7012 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2489 2657 2489 Size: 168 6844 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2034 2195 2736 2904 3156 3324 3660 3828 Size: 795 161 541 168 252 168 336 168 Cuts at: 3828 4080 4164 4248 5672 1239 Size: 252 84 84 1424 2579 Fragments arranged by size: 2579 1424 795 541 336 252 252 168 168 168 161 84 84 Bpu10I CC'TnA_GC Cuts at: 2038 2038 Size: 7012 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2759 5137 6002 6300 2759 Size: 2378 865 298 3471 Fragments arranged by size: 3471 2378 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2171 5675 882 Size: 617 672 3504 2219 Fragments arranged by size: 3504 2219 672 617 BsaAI yAC'GTr Cuts at: 493 1204 4159 4803 493 Size: 711 2955 644 2702 Fragments arranged by size: 2955 2702 711 644 BsaBI GATnn'nnATC Cuts at: 7006 7006 Size: 7012 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4819 5201 5836 275 Size: 53 83 186 936 3286 382 635 1451 Fragments arranged by size: 3286 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2630 3435 3974 5445 5843 6117 6264 6675 2630 Size: 805 539 1471 398 274 147 411 2967 Fragments arranged by size: 2967 1471 805 539 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6787 Fragments arranged by size: 6787 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7012 BseYI C'CCAG_C Cuts at: 6215 6711 6215 Size: 496 6516 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2923 4099 1163 Size: 573 1187 1176 4076 Fragments arranged by size: 4076 1187 1176 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4462 5223 6251 1540 Size: 275 186 2461 761 1028 2301 Fragments arranged by size: 2461 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5073 5158 6229 5073 Size: 85 1071 5856 Fragments arranged by size: 5856 1071 85 BsmI GAATG_Cn' Cuts at: 2402 2654 2738 2753 2831 3158 3425 3578 3662 Size: 252 84 15 78 327 267 153 84 Cuts at: 3662 3830 4523 4616 2402 Size: 168 693 93 4798 Fragments arranged by size: 4798 693 327 267 252 168 153 93 84 84 78 15 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2263 4331 5073 5158 6229 1391 Size: 399 473 2068 742 85 1071 2174 Fragments arranged by size: 2174 2068 1071 742 473 399 85 BspEI T'CCGG_A Cuts at: 6675 6675 Size: 7012 BspHI T'CATG_A Cuts at: 4901 6631 4901 Size: 1730 5282 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2199 3930 4367 844 Size: 1355 1731 437 3489 Fragments arranged by size: 3489 1731 1355 437 BsrBI CCG'CTC Cuts at: 1394 1823 4899 1394 Size: 429 3076 3507 Fragments arranged by size: 3507 3076 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2575 5507 5681 65 Size: 1183 1327 2932 174 1396 Fragments arranged by size: 2932 1396 1327 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3435 5656 5843 1700 Size: 110 1625 2221 187 2869 Fragments arranged by size: 2869 2221 1625 187 110 BsrGI T'GTAC_A Cuts at: 96 2506 96 Size: 2410 4602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7012 BssSI C'ACGA_G Cuts at: 5072 6084 5072 Size: 1012 6000 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7012 BstBI TT'CG_AA Cuts at: 5816 5816 Size: 7012 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7012 BstXI CCAn_nnnn'nTGG Cuts at: 2016 3668 2016 Size: 1652 5360 BstYI r'GATC_y Cuts at: 1972 2686 2807 4342 4709 5094 5111 6563 6649 Size: 714 121 1535 367 385 17 1452 86 Cuts at: 6649 6661 7007 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1535 1452 714 385 367 346 121 86 17 12 BstZ17I GTA'TAC Cuts at: 2696 2696 Size: 7012 BtgI C'CryG_G Cuts at: 513 1065 2255 513 Size: 552 1190 5270 Fragments arranged by size: 5270 1190 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6273 Fragments arranged by size: 6273 683 56 BtsI GCAGTG_nn' Cuts at: 2261 3163 3835 4087 4528 5322 5342 2261 Size: 902 672 252 441 794 20 3931 Fragments arranged by size: 3931 902 794 672 441 252 20 ClaI AT'CG_AT Cuts at: 1554 2106 1554 Size: 552 6460 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2557 2592 3397 3432 3565 3600 529 Size: 35 1993 35 805 35 133 35 3941 Fragments arranged by size: 3941 1993 805 133 35 35 35 35 DraI TTT'AAA Cuts at: 2563 4316 2563 Size: 1753 5259 DraIII CAC_nnn'GTG Cuts at: 3750 3750 Size: 7012 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6019 809 Size: 507 4703 1802 Fragments arranged by size: 4703 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4459 5348 6977 Size: 54 1425 50 275 18 2629 889 1629 Cuts at: 6977 8 Size: 43 Fragments arranged by size: 2629 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4459 1537 Size: 275 2647 4090 Fragments arranged by size: 4090 2647 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2612 3751 4942 1353 Size: 1259 1139 1191 3423 Fragments arranged by size: 3423 1259 1191 1139 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5599 5973 6119 387 Size: 1064 138 4010 374 146 1280 Fragments arranged by size: 4010 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2074 2195 2238 2593 2736 2904 Size: 393 402 40 121 43 355 143 168 Cuts at: 2904 2927 2992 3156 3324 3660 3828 4080 4164 Size: 23 65 164 168 336 168 252 84 Cuts at: 4164 4248 5075 5672 6459 6727 1239 Size: 84 827 597 787 268 1524 Fragments arranged by size: 1524 827 787 597 402 393 355 336 268 252 168 168 168 164 143 121 84 84 65 43 40 23 EcoRI G'AATT_C Cuts at: 2622 2874 3126 3210 3378 3798 4050 4134 4321 Size: 252 252 84 168 420 252 84 187 Cuts at: 4321 4699 2622 Size: 378 4935 Fragments arranged by size: 4935 420 378 252 252 252 187 168 84 84 EcoRV GAT'ATC Cuts at: 2207 2207 Size: 7012 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3292 3796 3292 Size: 504 6508 FspI TGC'GCA Cuts at: 5518 5518 Size: 7012 HaeII r_GCGC'y Cuts at: 1678 6159 1678 Size: 4481 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2011 2043 2266 2298 2799 Size: 32 135 32 439 32 223 32 501 Cuts at: 2799 2831 3103 3135 3271 3303 3523 3555 3750 Size: 32 272 32 136 32 220 32 195 Cuts at: 3750 3782 4027 4059 5653 5685 5727 5759 1373 Size: 32 245 32 1594 32 42 32 2626 Fragments arranged by size: 2626 1594 501 439 272 245 223 220 195 136 135 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4356 4602 669 Size: 988 281 2418 246 3079 Fragments arranged by size: 3079 2418 988 281 246 HindIII A'AGCT_T Cuts at: 748 2388 748 Size: 1640 5372 HpaI GTT'AAC Cuts at: 4602 4602 Size: 7012 KpnI G_GTAC'C Cuts at: 4337 4337 Size: 7012 MfeI C'AATT_G Cuts at: 4611 4611 Size: 7012 MluI A'CGCG_T Cuts at: 6888 6888 Size: 7012 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2217 3045 6126 6310 1305 Size: 912 828 3081 184 2007 Fragments arranged by size: 3081 2007 912 828 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5190 Fragments arranged by size: 5190 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2899 2955 2969 3321 3666 4970 5329 Size: 648 1733 56 14 352 345 1304 359 Cuts at: 5329 5488 518 Size: 159 2042 Fragments arranged by size: 2042 1733 1304 648 359 352 345 159 56 14 MspA1I CmG'CkG Cuts at: 1606 4428 5105 6253 6498 1606 Size: 2822 677 1148 245 2120 Fragments arranged by size: 2822 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2255 513 Size: 552 1190 5270 Fragments arranged by size: 5270 1190 552 NdeI CA'TA_TG Cuts at: 387 2544 2896 3068 387 Size: 2157 352 172 4331 Fragments arranged by size: 4331 2157 352 172 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7012 NotI GC'GGCC_GC Cuts at: 4459 4459 Size: 7012 NsiI A_TGCA'T Cuts at: 2486 2784 3242 2486 Size: 298 458 6256 Fragments arranged by size: 6256 458 298 NspI r_CATG'y Cuts at: 1132 1229 4370 5915 1132 Size: 97 3141 1545 2229 Fragments arranged by size: 3141 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7012 PciI A'CATG_T Cuts at: 5911 5911 Size: 7012 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7012 PmeI GTTT'AAAC Cuts at: 4316 4316 Size: 7012 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5081 5113 6594 6626 5081 Size: 32 1481 32 5467 Fragments arranged by size: 5467 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 4024 1613 Size: 2411 4601 PsiI TTA'TAA Cuts at: 2343 2679 4582 2343 Size: 336 1903 4773 Fragments arranged by size: 4773 1903 336 PstI C_TGCA'G Cuts at: 830 2268 4364 830 Size: 1438 2096 3478 Fragments arranged by size: 3478 2096 1438 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7012 SalI G'TCGA_C Cuts at: 1936 4354 1936 Size: 2418 4594 SapI GCTCTTCn'nnn_ Cuts at: 3751 3751 Size: 7012 SbfI CC_TGCA'GG Cuts at: 4364 4364 Size: 7012 ScaI AGT'ACT Cuts at: 5260 6972 5260 Size: 1712 5300 SfcI C'TryA_G Cuts at: 826 1045 2182 2264 3097 4360 5495 6176 6367 Size: 219 1137 82 833 1263 1135 681 191 Cuts at: 6367 826 Size: 1471 Fragments arranged by size: 1471 1263 1137 1135 833 681 219 191 82 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7012 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7012 SmaI CCC'GGG Cuts at: 1872 4339 1872 Size: 2467 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2774 5116 6017 6279 820 Size: 197 926 831 2342 901 262 1553 Fragments arranged by size: 2342 1553 926 901 831 262 197 SnaBI TAC'GTA Cuts at: 493 4803 493 Size: 4310 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7012 SphI G_CATG'C Cuts at: 1132 4370 1132 Size: 3238 3774 SspI AAT'ATT Cuts at: 5 52 4936 5 Size: 47 4884 2081 Fragments arranged by size: 4884 2081 47 StuI AGG'CCT Cuts at: 1517 2837 3089 3509 3929 4181 1517 Size: 1320 252 420 420 252 4348 Fragments arranged by size: 4348 1320 420 420 252 252 StyI C'CwwG_G Cuts at: 513 1065 2255 4451 513 Size: 552 1190 2196 3074 Fragments arranged by size: 3074 2196 1190 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2438 3530 5039 5056 5209 5394 5839 Size: 1255 858 1092 1509 17 153 185 445 Cuts at: 5839 325 Size: 1498 Fragments arranged by size: 1509 1498 1255 1092 858 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2443 2506 3071 Size: 274 80 33 51 1443 466 63 565 Cuts at: 3071 5258 6970 96 Size: 2187 1712 138 Fragments arranged by size: 2187 1712 1443 565 466 274 138 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6972 7004 6972 Size: 32 6980 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7012 XbaI T'CTAG_A Cuts at: 4348 4348 Size: 7012 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7012 XmaI C'CCGG_G Cuts at: 1870 4337 1870 Size: 2467 4545 XmnI GAAnn'nnTTC Cuts at: 2669 3005 3173 3425 3677 4097 4265 5141 2669 Size: 336 168 252 252 420 168 876 4540 Fragments arranged by size: 4540 876 420 336 252 252 168 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4820 276 Size: 53 83 186 936 3286 2468 Fragments arranged by size: 3286 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI