Restriction map for
pFN21AB6250
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaJI BseAI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StyI SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 284 GGTACC G^GTACC
AflIII 1426 ACATGT A^C[GA][CT]GT
AlwI 1787 GATCC GGATC(4/5)
AocI 230 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1772 AGCGCT AGC^GCT
Asp718I 284 GGTACC G^GTACC
AspI 3 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcnI 1194 CCGGG CC^[GC]GG
BsaWI 1903 ACCGGA [AT]^CCGG[AT]
BsgI 1589 GTGCAG GTGCAG(16/14)
Bsp1407I 1464 TGTACA T^GTACA
BspLU11I 1426 ACATGT A^CATGT
BspMI 287 ACCTGC ACCTGC(4/8)
BsrDI 1001 GCAATG GCAATG(2/0)
BsrGI 1464 TGTACA T^GTACA
BstF5I 197 GGATG GGATG(2/0)
BstNI 1362 CCAGG CC^[AT]GG
BstXI 1706 CCAATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 230 CCTCAGG CC^T[ACGT]AGG
Cac8I 957 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 1773 GCGC GCG^C
DraIII 1718 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1772 AGCGCT AGC^GCT
Eco81I 230 CCTCAGG CC^T[ACGT]AGG
EcoRII 1362 CCAGG ^CC[AT]GG
FokI 197 GGATG GGATG(9/13)
HaeII 1772 AGCGCT [GA]GCGC^[CT]
HhaI 1773 GCGC GCG^C
HinP1I 1773 GCGC G^CGC
KpnI 284 GGTACC GGTAC^C
MaeII 35 ACGT A^CGT
MvaI 1362 CCAGG CC^[AT]GG
MwoI 63 GCTGCAACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1194 CCGGG CC^[GC]GG
PflFI 3 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 955 CTGCAG CTGCA^G
SmlI 1956 CTCAAG C^T[CT][GA]AG
SspBI 1464 TGTACA T^GTACA
SspI 370 AATATT AAT^ATT
TaqI 2043 TCGA T^CGA
Tth111I 3 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2043 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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BanI 284 GGTACC G^G[CT][GA]CC
1643 GGTGCC
BbvI 63 GCTGC GCAGC(8/12)
386 GCTGC
BglII 317 AGATCT A^GATCT
1455 AGATCT
BsaI 1827 GAGACC GGTCTC(1/5)
132 GGTCTC
BspHI 294 TCATGA T^CATGA
963 TCATGA
BssKI 1194 CCGGG ^CC[ACGT]GG
1362 CCAGG
EcoT22I 1004 ATGCAT ATGCA^T
1256 ATGCAT
Fnu4HI 63 GCTGC GC^[ACGT]GC
386 GCTGC
HapII 1194 CCGG C^CGG
1904 CCGG
HpaII 1194 CCGG C^CGG
1904 CCGG
HphI 478 GGTGA GGTGA(8/7)
1999 GGTGA
ItaI 63 GCTGC GC^[ACGT]GC
386 GCTGC
MspA1I 519 CAGCTG C[AC]G^C[GT]G
1863 CAGCTG
MspI 1194 CCGG C^CGG
1904 CCGG
NsiI 1004 ATGCAT ATGCA^T
1256 ATGCAT
PvuII 519 CAGCTG CAG^CTG
1863 CAGCTG
RcaI 294 TCATGA T^CATGA
963 TCATGA
SapI 298 GAAGAGC GCTCTTC(1/4)
930 GAAGAGC
ScrFI 1194 CCGGG CC^[ACGT]GG
1362 CCAGG
SfcI 955 CTGCAG C^T[GA][CT]AG
1470 CTGTAG
StuI 1364 AGGCCT AGG^CCT
1520 AGGCCT
TseI 63 GCTGC G^C[AT]GC
386 GCTGC
Tsp509I 540 AATT ^AATT
1708 AATT
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI Asp700I AspHI AvaII
BanII BbsI BmyI BpmI BpuAI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BstYI Cfr13I DdeI
DpnII EarI HaeIII HinfI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MslI NdeII NlaIII NlaIV
NspI PleI RsaI SacI Sau3AI
Sau96I SfaNI TfiI Tru9I Tsp45I
TspRI XhoII XmnI