(Circular) (Six-base) MAPSORT of: pFN21AB6250.seq Check: 5527 from: 1 to: 6751 pFN21AB6250 6751 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4561 278 Size: 53 83 186 936 3025 2468 Fragments arranged by size: 3025 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4094 1937 Size: 2157 4594 Acc65I G'GTAC_C Cuts at: 2289 4072 2289 Size: 1783 4968 AclI AA'CG_TT Cuts at: 1563 4878 5251 1563 Size: 3315 373 3063 Fragments arranged by size: 3315 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2110 2842 2926 3113 3262 3346 3430 3514 Size: 478 732 84 187 149 84 84 84 Cuts at: 3514 3533 3598 3766 3850 3934 4814 6198 6466 Size: 19 65 168 84 84 880 1384 268 Cuts at: 6466 1632 Size: 1917 Fragments arranged by size: 1917 1384 880 732 478 268 187 168 149 84 84 84 84 84 84 65 19 AfeI AGC'GCT Cuts at: 3779 3779 Size: 6751 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6554 AflIII A'CryG_T Cuts at: 1203 3431 5650 6627 1203 Size: 2228 2219 977 1327 Fragments arranged by size: 2228 2219 1327 977 AgeI A'CCGG_T Cuts at: 5582 5582 Size: 6751 AhdI GACnn_n'nnGTC Cuts at: 5480 5480 Size: 6751 AleI CACnn'nnGTG Cuts at: 1166 2481 1166 Size: 1315 5436 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6751 AlwNI CAG_nnn'CTG Cuts at: 4494 6066 4494 Size: 1572 5179 ApaLI G'TGCA_C Cuts at: 4808 5964 4808 Size: 1156 5595 ApoI r'AATT_y Cuts at: 1493 4060 4286 4438 6547 1493 Size: 2567 226 152 2109 1697 Fragments arranged by size: 2567 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5305 160 Size: 872 4273 1606 Fragments arranged by size: 4273 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6751 AvaI C'yCGr_G Cuts at: 1870 1943 4047 4076 1870 Size: 73 2104 29 4545 Fragments arranged by size: 4545 2104 73 29 BamHI G'GATC_C Cuts at: 4081 4448 4081 Size: 367 6384 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2289 3648 4072 5527 618 Size: 325 217 627 502 1359 424 1455 1842 Fragments arranged by size: 1842 1455 1359 627 502 424 325 217 BanII G_rGCy'C Cuts at: 1391 2534 2560 3458 3878 3962 4070 1391 Size: 1143 26 898 420 84 108 4072 Fragments arranged by size: 4072 1143 898 420 108 84 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2044 2615 3856 928 Size: 971 145 571 1241 3823 Fragments arranged by size: 3823 1241 971 571 145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4942 4976 1903 Size: 34 3005 34 3678 Fragments arranged by size: 3678 3005 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4643 5853 4643 Size: 1210 5541 BclI T'GATC_A Cuts at: 1548 1548 Size: 6751 BfrBI ATG'CAT Cuts at: 3011 3263 3011 Size: 252 6499 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5362 136 Size: 107 122 71 631 605 3690 1525 Fragments arranged by size: 3690 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2322 3460 6746 2322 Size: 1138 3286 2327 Fragments arranged by size: 3286 2327 1138 BlpI GC'TnA_GC Cuts at: 4168 4168 Size: 6751 Bme1580I G_kGCm'C Cuts at: 1790 4812 5968 1790 Size: 3022 1156 2573 Fragments arranged by size: 3022 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2041 1318 Size: 723 6028 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4482 5440 442 Size: 416 53 83 399 24 3065 958 1753 Fragments arranged by size: 3065 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6751 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2548 2754 3174 3258 3426 3526 3594 Size: 899 410 206 420 84 168 100 68 Cuts at: 3594 3678 3892 5411 1239 Size: 84 214 1519 2579 Fragments arranged by size: 2579 1519 899 420 410 214 206 168 100 84 84 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3946 4876 5741 6039 3946 Size: 930 865 298 4658 Fragments arranged by size: 4658 930 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2143 3826 5414 882 Size: 617 644 1683 1588 2219 Fragments arranged by size: 2219 1683 1588 644 617 BsaAI yAC'GTr Cuts at: 493 1204 4542 493 Size: 711 3338 2702 Fragments arranged by size: 3338 2702 711 BsaBI GATnn'nnATC Cuts at: 6745 6745 Size: 6751 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4558 4940 5575 275 Size: 53 83 186 936 3025 382 635 1451 Fragments arranged by size: 3025 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3908 5184 5582 5856 6003 6414 3908 Size: 1276 398 274 147 411 4245 Fragments arranged by size: 4245 1276 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2677 2707 3949 3979 1178 Size: 30 165 30 1274 30 1242 30 3950 Fragments arranged by size: 3950 1274 1242 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2078 2224 2790 2855 3107 3191 3359 Size: 889 3 146 566 65 252 84 168 Cuts at: 3359 3695 3947 1186 Size: 336 252 3990 Fragments arranged by size: 3990 889 566 336 252 252 168 146 84 65 3 BseYI C'CCAG_C Cuts at: 5954 6450 5954 Size: 496 6255 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3615 1163 Size: 573 1879 4299 Fragments arranged by size: 4299 1879 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4201 4962 5990 1540 Size: 275 186 2200 761 1028 2301 Fragments arranged by size: 2301 2200 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2534 3458 3878 3962 4812 4897 5968 2534 Size: 924 420 84 850 85 1071 3317 Fragments arranged by size: 3317 1071 924 850 420 85 84 BsmI GAATG_Cn' Cuts at: 4262 4355 4262 Size: 93 6658 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2534 2560 3458 3878 3962 4070 4812 Size: 399 744 26 898 420 84 108 742 Cuts at: 4812 4897 5968 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 898 744 742 420 399 108 85 84 26 BspEI T'CCGG_A Cuts at: 6414 6414 Size: 6751 BspHI T'CATG_A Cuts at: 2299 2968 4640 6370 2299 Size: 669 1672 1730 2680 Fragments arranged by size: 2680 1730 1672 669 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2301 4106 844 Size: 1457 1805 3489 Fragments arranged by size: 3489 1805 1457 BsrBI CCG'CTC Cuts at: 1394 1823 4638 1394 Size: 429 2815 3507 Fragments arranged by size: 3507 2815 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3013 5246 5420 65 Size: 1183 1765 2233 174 1396 Fragments arranged by size: 2233 1765 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5395 5582 1700 Size: 110 3585 187 2869 Fragments arranged by size: 3585 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3469 96 Size: 3373 3378 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6751 BssSI C'ACGA_G Cuts at: 4811 5823 4811 Size: 1012 5739 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6751 BstBI TT'CG_AA Cuts at: 5555 5555 Size: 6751 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6751 BstXI CCAn_nnnn'nTGG Cuts at: 3718 3718 Size: 6751 BstYI r'GATC_y Cuts at: 1972 2322 3460 3791 4081 4448 4833 4850 6302 Size: 350 1138 331 290 367 385 17 1452 Cuts at: 6302 6388 6400 6746 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1138 385 367 350 346 331 290 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2236 2236 Size: 6751 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6199 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6012 Fragments arranged by size: 6012 683 56 BtsI GCAGTG_nn' Cuts at: 2031 4267 5061 5081 2031 Size: 2236 794 20 3701 Fragments arranged by size: 3701 2236 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6751 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2995 3030 529 Size: 35 2431 35 4250 Fragments arranged by size: 4250 2431 35 35 DraI TTT'AAA Cuts at: 4055 4055 Size: 6751 DraIII CAC_nnn'GTG Cuts at: 3728 3728 Size: 6751 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5758 809 Size: 507 4442 1802 Fragments arranged by size: 4442 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4198 5087 6716 Size: 54 1425 50 275 18 2368 889 1629 Cuts at: 6716 8 Size: 43 Fragments arranged by size: 2368 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4198 1537 Size: 275 2386 4090 Fragments arranged by size: 4090 2386 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2274 2298 2751 2919 2930 4681 1353 Size: 921 24 453 168 11 1751 3423 Fragments arranged by size: 3423 1751 921 453 168 24 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5338 5712 5858 387 Size: 1064 138 3749 374 146 1280 Fragments arranged by size: 3749 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2532 3456 3876 3960 2532 Size: 924 420 84 5323 Fragments arranged by size: 5323 924 420 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 2138 2548 2754 2842 2926 3113 Size: 393 478 28 410 206 88 84 187 Cuts at: 3113 3174 3258 3262 3346 3426 3430 3514 3526 Size: 61 84 4 84 80 4 84 12 Cuts at: 3526 3533 3594 3598 3678 3766 3850 3892 3934 Size: 7 61 4 80 88 84 42 42 Cuts at: 3934 4814 5411 6198 6466 1239 Size: 880 597 787 268 1524 Fragments arranged by size: 1524 880 787 597 478 410 393 268 206 187 88 88 84 84 84 84 84 80 80 61 61 42 42 28 12 7 4 4 4 EcoRI G'AATT_C Cuts at: 4060 4438 4060 Size: 378 6373 FspI TGC'GCA Cuts at: 5257 5257 Size: 6751 HaeII r_GCGC'y Cuts at: 1678 3781 5898 1678 Size: 2103 2117 2531 Fragments arranged by size: 2531 2117 2103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2251 2283 2334 2366 Size: 32 135 32 647 32 32 51 32 Cuts at: 2366 2440 2472 2521 2553 2677 2689 2709 2721 Size: 74 32 49 32 124 12 20 12 Cuts at: 2721 2857 2889 3445 3477 3949 3981 5392 5424 Size: 136 32 556 32 472 32 1411 32 Cuts at: 5424 5466 5498 1373 Size: 42 32 2626 Fragments arranged by size: 2626 1411 647 556 472 136 135 124 74 51 49 42 32 32 32 32 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 4095 4341 669 Size: 988 281 2157 246 3079 Fragments arranged by size: 3079 2157 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6751 HpaI GTT'AAC Cuts at: 4341 4341 Size: 6751 KpnI G_GTAC'C Cuts at: 2293 4076 2293 Size: 1783 4968 MfeI C'AATT_G Cuts at: 4350 4350 Size: 6751 MluI A'CGCG_T Cuts at: 6627 6627 Size: 6751 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2379 3431 3935 5865 6049 1305 Size: 1074 1052 504 1930 184 2007 Fragments arranged by size: 2007 1930 1074 1052 504 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4929 Fragments arranged by size: 4929 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2481 3001 3169 3253 3589 3757 3841 Size: 648 1315 520 168 84 336 168 84 Cuts at: 3841 3925 3993 4709 5068 5227 518 Size: 84 68 716 359 159 2042 Fragments arranged by size: 2042 1315 716 648 520 359 336 168 168 159 84 84 84 68 MspA1I CmG'CkG Cuts at: 1606 2526 3870 4167 4844 5992 6237 1606 Size: 920 1344 297 677 1148 245 2120 Fragments arranged by size: 2120 1344 1148 920 677 297 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6199 NdeI CA'TA_TG Cuts at: 387 387 Size: 6751 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6751 NotI GC'GGCC_GC Cuts at: 4198 4198 Size: 6751 NsiI A_TGCA'T Cuts at: 3013 3265 3013 Size: 252 6499 NspI r_CATG'y Cuts at: 1132 1229 3015 3267 3435 4109 5654 1132 Size: 97 1786 252 168 674 1545 2229 Fragments arranged by size: 2229 1786 1545 674 252 168 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6751 PciI A'CATG_T Cuts at: 3431 5650 3431 Size: 2219 4532 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6751 PmeI GTTT'AAAC Cuts at: 4055 4055 Size: 6751 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4820 4852 6333 6365 4820 Size: 32 1481 32 5206 Fragments arranged by size: 5206 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6751 PsiI TTA'TAA Cuts at: 4321 4321 Size: 6751 PspXI vC'TCGA_Gb Cuts at: 4047 4047 Size: 6751 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2434 2466 2434 Size: 32 6719 PstI C_TGCA'G Cuts at: 830 2964 4103 830 Size: 2134 1139 3478 Fragments arranged by size: 3478 2134 1139 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6751 PvuII CAG'CTG Cuts at: 2526 3870 2526 Size: 1344 5407 SacI G_AGCT'C Cuts at: 2534 3458 3878 3962 2534 Size: 924 420 84 5323 Fragments arranged by size: 5323 924 420 84 SalI G'TCGA_C Cuts at: 1936 4093 1936 Size: 2157 4594 SapI GCTCTTCn'nnn_ Cuts at: 2298 2930 2298 Size: 632 6119 SbfI CC_TGCA'GG Cuts at: 4103 4103 Size: 6751 ScaI AGT'ACT Cuts at: 4999 6711 4999 Size: 1712 5039 SfcI C'TryA_G Cuts at: 826 1045 2960 3475 4099 5234 5915 6106 826 Size: 219 1915 515 624 1135 681 191 1471 Fragments arranged by size: 1915 1471 1135 681 624 515 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6751 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6751 SmaI CCC'GGG Cuts at: 1872 4078 1872 Size: 2206 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3961 4047 4855 5756 6018 820 Size: 197 926 2018 86 808 901 262 1553 Fragments arranged by size: 2018 1553 926 901 808 262 197 86 SnaBI TAC'GTA Cuts at: 493 4542 493 Size: 4049 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6751 SphI G_CATG'C Cuts at: 1132 4109 1132 Size: 2977 3774 SspI AAT'ATT Cuts at: 5 52 2377 4675 5 Size: 47 2325 2298 2081 Fragments arranged by size: 2325 2298 2081 47 StuI AGG'CCT Cuts at: 1517 3371 3527 1517 Size: 1854 156 4741 Fragments arranged by size: 4741 1854 156 StyI C'CwwG_G Cuts at: 513 1065 4190 513 Size: 552 3125 3074 Fragments arranged by size: 3125 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4778 4795 4948 5133 5578 325 Size: 1255 3198 17 153 185 445 1498 Fragments arranged by size: 3198 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3469 4997 6709 Size: 274 80 33 51 1443 1492 1528 1712 Cuts at: 6709 96 Size: 138 Fragments arranged by size: 1712 1528 1492 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6711 6743 6711 Size: 32 6719 Tth111I GACn'n_nGTC Cuts at: 1316 2011 1316 Size: 695 6056 XbaI T'CTAG_A Cuts at: 4087 4087 Size: 6751 XhoI C'TCGA_G Cuts at: 1943 4047 1943 Size: 2104 4647 XmaI C'CCGG_G Cuts at: 1870 4076 1870 Size: 2206 4545 XmnI GAAnn'nnTTC Cuts at: 2519 2939 3107 3191 3443 3611 3695 3863 3947 Size: 420 168 84 252 168 84 168 84 Cuts at: 3947 4880 2519 Size: 933 4390 Fragments arranged by size: 4390 933 420 252 168 168 168 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4559 276 Size: 53 83 186 936 3025 2468 Fragments arranged by size: 3025 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI