Restriction map for
pFN21AB6250
(Linear) (Six-base) MAPSORT of: KIBB6250.seq Check: 5712 from: 1 to: 2046
KIBB6250.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
Acc65I G'GTAC_C
Cuts at: 0 284 2046
Size: 284 1762
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 837 921 1108 1257 1341 1425 1509
Size: 105 732 84 187 149 84 84 84
Cuts at: 1509 1528 1593 1761 1845 1929 2046
Size: 19 65 168 84 84 117
Fragments arranged by size:
732 187 168 149 117 105 84 84
84 84 84 84 65 19
AfeI AGC'GCT
Cuts at: 0 1774 2046
Size: 1774 272
AflIII A'CryG_T
Cuts at: 0 1426 2046
Size: 1426 620
AleI CACnn'nnGTG
Cuts at: 0 476 2046
Size: 476 1570
BanI G'GyrC_C
Cuts at: 0 284 1643 2046
Size: 284 1359 403
Fragments arranged by size:
1359 403 284
BanII G_rGCy'C
Cuts at: 0 529 555 1453 1873 1957 2046
Size: 529 26 898 420 84 89
Fragments arranged by size:
898 529 420 89 84 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 39 610 1851 2046
Size: 39 571 1241 195
Fragments arranged by size:
1241 571 195 39
BfrBI ATG'CAT
Cuts at: 0 1006 1258 2046
Size: 1006 252 788
Fragments arranged by size:
1006 788 252
BglII A'GATC_T
Cuts at: 0 317 1455 2046
Size: 317 1138 591
Fragments arranged by size:
1138 591 317
BmgBI CAC'GTC
Cuts at: 0 36 2046
Size: 36 2010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 543 749 1169 1253 1421 1521 1589
Size: 133 410 206 420 84 168 100 68
Cuts at: 1589 1673 1887 2046
Size: 84 214 159
Fragments arranged by size:
420 410 214 206 168 159 133 100
84 84 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1941 2046
Size: 1941 105
BsaI GGTCTCn'nnnn_
Cuts at: 0 138 1821 2046
Size: 138 1683 225
Fragments arranged by size:
1683 225 138
BsaWI w'CCGG_w
Cuts at: 0 1903 2046
Size: 1903 143
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 672 702 1944 1974 2046
Size: 672 30 1242 30 72
Fragments arranged by size:
1242 672 72 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 73 219 785 850 1102 1186 1354
Size: 70 3 146 566 65 252 84 168
Cuts at: 1354 1690 1942 2046
Size: 336 252 104
Fragments arranged by size:
566 336 252 252 168 146 104 84
70 65 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1610 2046
Size: 1610 436
BsiHKAI G_wGCw'C
Cuts at: 0 529 1453 1873 1957 2046
Size: 529 924 420 84 89
Fragments arranged by size:
924 529 420 89 84
Bsp1286I G_dGCh'C
Cuts at: 0 529 555 1453 1873 1957 2046
Size: 529 26 898 420 84 89
Fragments arranged by size:
898 529 420 89 84 26
BspHI T'CATG_A
Cuts at: 0 294 963 2046
Size: 294 669 1083
Fragments arranged by size:
1083 669 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 296 2046
Size: 296 1750
BsrDI GCAATG_nn'
Cuts at: 0 1008 2046
Size: 1008 1038
BsrGI T'GTAC_A
Cuts at: 0 1464 2046
Size: 1464 582
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1713 2046
Size: 1713 333
BstYI r'GATC_y
Cuts at: 0 317 1455 1786 2046
Size: 317 1138 331 260
Fragments arranged by size:
1138 331 317 260
Bsu36I CC'TnA_GG
Cuts at: 0 231 2046
Size: 231 1815
BtsI GCAGTG_nn'
Cuts at: 0 26 2046
Size: 26 2020
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 990 1025 2046
Size: 990 35 1021
Fragments arranged by size:
1021 990 35
DraIII CAC_nnn'GTG
Cuts at: 0 1723 2046
Size: 1723 323
EarI CTCTTCn'nnn_
Cuts at: 0 269 293 746 914 925 2046
Size: 269 24 453 168 11 1121
Fragments arranged by size:
1121 453 269 168 24 11
EcoICRI GAG'CTC
Cuts at: 0 527 1451 1871 1955 2046
Size: 527 924 420 84 91
Fragments arranged by size:
924 527 420 91 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 133 543 749 837 921 1108 1169
Size: 105 28 410 206 88 84 187 61
Cuts at: 1169 1253 1257 1341 1421 1425 1509 1521 1528
Size: 84 4 84 80 4 84 12 7
Cuts at: 1528 1589 1593 1673 1761 1845 1887 1929 2046
Size: 61 4 80 88 84 42 42 117
Fragments arranged by size:
410 206 187 117 105 88 88 84
84 84 84 84 80 80 61 61
42 42 28 12 7 4 4 4
HaeII r_GCGC'y
Cuts at: 0 1776 2046
Size: 1776 270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 278 329 361 435 467 516
Size: 214 32 32 51 32 74 32 49
Cuts at: 516 548 672 684 704 716 852 884 1440
Size: 32 124 12 20 12 136 32 556
Cuts at: 1440 1472 1944 1976 2046
Size: 32 472 32 70
Fragments arranged by size:
556 472 214 136 124 74 70 51
49 32 32 32 32 32 32 32
32 20 12 12
KpnI G_GTAC'C
Cuts at: 0 288 2046
Size: 288 1758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1426 1930 2046
Size: 374 1052 504 116
Fragments arranged by size:
1052 504 374 116
MslI CAynn'nnrTG
Cuts at: 0 476 996 1164 1248 1584 1752 1836 1920
Size: 476 520 168 84 336 168 84 84
Cuts at: 1920 1988 2046
Size: 68 58
Fragments arranged by size:
520 476 336 168 168 84 84 84
68 58
MspA1I CmG'CkG
Cuts at: 0 521 1865 2046
Size: 521 1344 181
Fragments arranged by size:
1344 521 181
NsiI A_TGCA'T
Cuts at: 0 1008 1260 2046
Size: 1008 252 786
Fragments arranged by size:
1008 786 252
NspI r_CATG'y
Cuts at: 0 1010 1262 1430 2046
Size: 1010 252 168 616
Fragments arranged by size:
1010 616 252 168
PciI A'CATG_T
Cuts at: 0 1426 2046
Size: 1426 620
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 429 461 2046
Size: 429 32 1585
Fragments arranged by size:
1585 429 32
PstI C_TGCA'G
Cuts at: 0 959 2046
Size: 959 1087
PvuII CAG'CTG
Cuts at: 0 521 1865 2046
Size: 521 1344 181
Fragments arranged by size:
1344 521 181
SacI G_AGCT'C
Cuts at: 0 529 1453 1873 1957 2046
Size: 529 924 420 84 89
Fragments arranged by size:
924 529 420 89 84
SapI GCTCTTCn'nnn_
Cuts at: 0 293 925 2046
Size: 293 632 1121
Fragments arranged by size:
1121 632 293
SfcI C'TryA_G
Cuts at: 0 955 1470 2046
Size: 955 515 576
Fragments arranged by size:
955 576 515
SmlI C'TyrA_G
Cuts at: 0 1956 2046
Size: 1956 90
SspI AAT'ATT
Cuts at: 0 372 2046
Size: 372 1674
StuI AGG'CCT
Cuts at: 0 1366 1522 2046
Size: 1366 156 524
Fragments arranged by size:
1366 524 156
TatI w'GTAC_w
Cuts at: 0 1464 2046
Size: 1464 582
Tth111I GACn'n_nGTC
Cuts at: 0 6 2046
Size: 6 2040
XmnI GAAnn'nnTTC
Cuts at: 0 514 934 1102 1186 1438 1606 1690 1858
Size: 514 420 168 84 252 168 84 168
Cuts at: 1858 1942 2046
Size: 84 104
Fragments arranged by size:
514 420 252 168 168 168 104 84
84 84
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AleI BanI BanII BbsI
BfrBI BglII BmgBI BpmI BpuEI BsaI BsaWI BsaXI
BseRI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI
BstXI BstYI Bsu36I BtsI CspCI DraIII EarI EcoICRI
Eco57MI HaeII Hin4I KpnI MmeI MslI MspA1I NsiI
NspI PciI PsrI PstI PvuII SacI SapI SfcI
SmlI SspI StuI TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BglI BlpI Bme1580I BmrI BmtI BplI Bpu10I
BsaAI BsaBI BsaHI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgI BtgZI ClaI DraI DrdI EaeI EagI
EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StyI SwaI TaqII
TstI XbaI XcmI XhoI XmaI ZraI