Restriction map for
pFN21AB6248
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NarI NciI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 989 TCCGGA T^CCGGA
AcyI 766 GACGCC G[GA]^CG[CT]C
AfaI 1347 GTAC GT^AC
AflIII 619 ACGTGT A^C[GA][CT]GT
AgeI 1935 ACCGGT A^CCGGT
AvaI 843 CTCGGG C^[CT]CG[GA]G
AvaII 198 GGTCC G^G[AT]CC
AviII 1073 TGCGCA TGC^GCA
BamHI 668 GGATCC G^GATCC
BanII 33 GGGCTC G[GA]GC[CT]^C
BbsI 1939 GTCTTC GAAGAC(2/6)
BciVI 1088 GTATCC GTATCC(6/5)
BfaI 1207 CTAG C^TAG
BglII 701 AGATCT A^GATCT
BpuAI 1939 GTCTTC GAAGAC(2/6)
BsaHI 766 GACGCC G[GA]^CG[CT]C
BsaI 268 GAGACC GGTCTC(1/5)
BseAI 989 TCCGGA T^CCGGA
BsiEI 1101 CGACCG CG[GA][CT]^CG
BsmI 1039 GAATGC GAATGC(1/-1)
BsoBI 843 CTCGGG C^[CT]CG[GA]G
BspEI 989 TCCGGA T^CCGGA
DraI 495 TTTAAA TTT^AAA
EcoNI 1626 CCTGCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1073 TGCGCA TGC^GCA
HgaI 766 GACGC GACGC(5/10)
Hin1I 766 GACGCC G[GA]^CG[CT]C
MaeI 1207 CTAG C^TAG
MroI 989 TCCGGA T^CCGGA
NcoI 1179 CCATGG C^CATGG
PinAI 1935 ACCGGT A^CCGGT
PvuII 163 CAGCTG CAG^CTG
RsaI 1347 GTAC GT^AC
SmlI 904 CTCAAG C^T[CT][GA]AG
StuI 1845 AGGCCT AGG^CCT
TfiI 1860 GATTC G^A[AT]TC
XbaI 1206 TCTAGA T^CTAGA
XcmI 1782 CCAGGAAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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Alw44I 838 GTGCAC G^TGCAC
1006 GTGCAC
AocI 663 CCTGAGG CC^T[ACGT]AGG
1769 CCTCAGG
ApaLI 838 GTGCAC G^TGCAC
1006 GTGCAC
BalI 1247 TGGCCA TGG^CCA
1919 TGGCCA
BglI 587 GCCACACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1573 GCCTTGGTGGC
BsaWI 989 TCCGGA [AT]^CCGG[AT]
1935 ACCGGT
BseRI 1944 CTCCTC GAGGAG(10/8)
1947 CTCCTC
BsrBI 487 GAGCGG CCGCTC(-3/-3)
682 GAGCGG
BssSI 1081 CACGAG CACGAG(-5/-1)
1720 CACGAG
Bsu36I 663 CCTGAGG CC^T[ACGT]AGG
1769 CCTCAGG
EaeI 1247 TGGCCA [CT]^GGCC[GA]
1919 TGGCCA
Eco81I 663 CCTGAGG CC^T[ACGT]AGG
1769 CCTCAGG
EcoT14I 1179 CCATGG C^C[AT][AT]GG
1574 CCTTGG
HaeII 793 AGCGCC [GA]GCGC^[CT]
1929 AGCGCC
MaeII 619 ACGT A^CGT
1370 ACGT
MluNI 1247 TGGCCA TGG^CCA
1919 TGGCCA
MscI 1247 TGGCCA TGG^CCA
1919 TGGCCA
NspI 578 ACATGC [GA]CATG^[CT]
830 ACATGC
StyI 1179 CCATGG C^C[AT][AT]GG
1574 CCTTGG
TaqI 1133 TCGA T^CGA
1394 TCGA
TthHB8I 1133 TCGA T^CGA
1394 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI Asp700I AspHI BanI
BbvI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NdeII NgoMIV NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI