(Circular) (Six-base) MAPSORT of: pFN21AB6248.seq Check: 5256 from: 1 to: 6748 pFN21AB6248 6748 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2428 2760 3238 2428 Size: 332 478 5938 Fragments arranged by size: 5938 478 332 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4558 278 Size: 53 83 186 936 3022 2468 Fragments arranged by size: 3022 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4091 1937 Size: 2154 4594 Acc65I G'GTAC_C Cuts at: 4069 4069 Size: 6748 AclI AA'CG_TT Cuts at: 1563 4875 5248 1563 Size: 3312 373 3063 Fragments arranged by size: 3312 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4811 6195 6463 1632 Size: 3179 1384 268 1917 Fragments arranged by size: 3179 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6551 AflIII A'CryG_T Cuts at: 1203 2624 5647 6624 1203 Size: 1421 3023 977 1327 Fragments arranged by size: 3023 1421 1327 977 AgeI A'CCGG_T Cuts at: 3940 5579 3940 Size: 1639 5109 AhdI GACnn_n'nnGTC Cuts at: 5477 5477 Size: 6748 AleI CACnn'nnGTG Cuts at: 1166 2066 2600 1166 Size: 900 534 5314 Fragments arranged by size: 5314 900 534 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2084 1184 Size: 900 5848 AlwNI CAG_nnn'CTG Cuts at: 2231 2369 3631 4491 6063 2231 Size: 138 1262 860 1572 2916 Fragments arranged by size: 2916 1572 1262 860 138 ApaLI G'TGCA_C Cuts at: 2843 3011 4805 5961 2843 Size: 168 1794 1156 3630 Fragments arranged by size: 3630 1794 1156 168 ApoI r'AATT_y Cuts at: 1493 2049 2303 2505 4057 4283 4435 6544 1493 Size: 556 254 202 1552 226 152 2109 1697 Fragments arranged by size: 2109 1697 1552 556 254 226 202 152 AseI AT'TA_AT Cuts at: 160 1032 5302 160 Size: 872 4270 1606 Fragments arranged by size: 4270 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6748 AvaI C'yCGr_G Cuts at: 1870 1943 2848 4073 1870 Size: 73 905 1225 4545 Fragments arranged by size: 4545 1225 905 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2908 2941 3076 3109 2908 Size: 33 135 33 6547 Fragments arranged by size: 6547 135 33 33 BamHI G'GATC_C Cuts at: 2673 4078 4445 2673 Size: 1405 367 4976 Fragments arranged by size: 4976 1405 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2589 3448 3517 4069 5524 Size: 325 217 627 802 859 69 552 1455 Cuts at: 5524 618 Size: 1842 Fragments arranged by size: 1842 1455 859 802 627 552 325 217 69 BanII G_rGCy'C Cuts at: 1391 2042 4067 1391 Size: 651 2025 4072 Fragments arranged by size: 4072 2025 651 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3937 928 Size: 971 2038 3739 Fragments arranged by size: 3739 2038 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4939 4973 1903 Size: 34 3002 34 3678 Fragments arranged by size: 3678 3002 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3104 4640 5850 3104 Size: 1536 1210 4002 Fragments arranged by size: 4002 1536 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6748 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2598 3584 5359 Size: 107 122 71 631 605 926 986 1775 Cuts at: 5359 136 Size: 1525 Fragments arranged by size: 1775 1525 986 926 631 605 122 107 71 BglII A'GATC_T Cuts at: 2706 6743 2706 Size: 4037 2711 BlpI GC'TnA_GC Cuts at: 4165 4165 Size: 6748 Bme1580I G_kGCm'C Cuts at: 1790 2847 3015 3453 3474 3522 4809 5965 1790 Size: 1057 168 438 21 48 1287 1156 2573 Fragments arranged by size: 2573 1287 1156 1057 438 168 48 21 BmgBI CAC'GTC Cuts at: 1318 2625 3376 1318 Size: 1307 751 4690 Fragments arranged by size: 4690 1307 751 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3469 4479 5437 Size: 416 53 83 399 24 2052 1010 958 Cuts at: 5437 442 Size: 1753 Fragments arranged by size: 2052 1753 1010 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6748 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3055 3055 Size: 6748 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2283 2716 3046 3371 3428 3440 5408 1239 Size: 1044 433 330 325 57 12 1968 2579 Fragments arranged by size: 2579 1968 1044 433 330 325 57 12 Bpu10I CC'TnA_GC Cuts at: 3161 3161 Size: 6748 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2894 4873 5738 6036 2894 Size: 1979 865 298 3606 Fragments arranged by size: 3606 1979 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2267 5411 882 Size: 617 768 3144 2219 Fragments arranged by size: 3144 2219 768 617 BsaAI yAC'GTr Cuts at: 493 1204 4539 493 Size: 711 3335 2702 Fragments arranged by size: 3335 2702 711 BsaBI GATnn'nnATC Cuts at: 6742 6742 Size: 6748 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2772 4555 4937 5572 Size: 53 83 186 936 1239 1783 382 635 Cuts at: 5572 275 Size: 1451 Fragments arranged by size: 1783 1451 1239 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2994 3940 5181 5579 5853 6000 6411 2994 Size: 946 1241 398 274 147 411 3331 Fragments arranged by size: 3331 1241 946 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3044 3074 1178 Size: 30 165 30 1641 30 4852 Fragments arranged by size: 4852 1641 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3940 3943 1186 Size: 2754 3 3991 Fragments arranged by size: 3991 2754 3 BseYI C'CCAG_C Cuts at: 2195 3152 3404 3461 3699 3960 5951 6447 2195 Size: 957 252 57 238 261 1991 496 2496 Fragments arranged by size: 2496 1991 957 496 261 252 238 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2875 3085 3734 1163 Size: 573 1139 210 649 4177 Fragments arranged by size: 4177 1139 649 573 210 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 4198 4959 5987 1540 Size: 275 186 1108 1089 761 1028 2301 Fragments arranged by size: 2301 1108 1089 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2847 3015 3041 4809 4894 5965 2847 Size: 168 26 1768 85 1071 3630 Fragments arranged by size: 3630 1768 1071 168 85 26 BsmI GAATG_Cn' Cuts at: 3050 4259 4352 3050 Size: 1209 93 5446 Fragments arranged by size: 5446 1209 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2847 3015 3041 3453 3474 3522 Size: 399 252 805 168 26 412 21 48 Cuts at: 3522 4067 4809 4894 5965 1391 Size: 545 742 85 1071 2174 Fragments arranged by size: 2174 1071 805 742 545 412 399 252 168 85 48 26 21 BspEI T'CCGG_A Cuts at: 2994 6411 2994 Size: 3417 3331 BspHI T'CATG_A Cuts at: 4637 6367 4637 Size: 1730 5018 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2343 2428 2760 3238 4103 844 Size: 1499 85 332 478 865 3489 Fragments arranged by size: 3489 1499 865 478 332 85 BsrBI CCG'CTC Cuts at: 1394 1823 2494 2689 4635 1394 Size: 429 671 195 1946 3507 Fragments arranged by size: 3507 1946 671 429 195 BsrDI GCAATG_nn' Cuts at: 65 1248 2887 2959 3699 5243 5417 65 Size: 1183 1639 72 740 1544 174 1396 Fragments arranged by size: 1639 1544 1396 1183 740 174 72 BsrFI r'CCGG_y Cuts at: 1700 1810 3227 3243 3618 3703 3940 5392 5579 Size: 110 1417 16 375 85 237 1452 187 Cuts at: 5579 1700 Size: 2869 Fragments arranged by size: 2869 1452 1417 375 237 187 110 85 16 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6748 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6748 BssSI C'ACGA_G Cuts at: 3086 3725 4808 5820 3086 Size: 639 1083 1012 4014 Fragments arranged by size: 4014 1083 1012 639 BstAPI GCAn_nnn'nTGC Cuts at: 794 3129 3517 3631 794 Size: 2335 388 114 3911 Fragments arranged by size: 3911 2335 388 114 BstBI TT'CG_AA Cuts at: 5552 5552 Size: 6748 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6748 BstYI r'GATC_y Cuts at: 1972 2673 2706 3627 4078 4445 4830 4847 6299 Size: 701 33 921 451 367 385 17 1452 Cuts at: 6299 6385 6397 6743 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 921 701 451 385 367 346 86 33 17 12 Bsu36I CC'TnA_GG Cuts at: 2669 3775 2669 Size: 1106 5642 BtgI C'CryG_G Cuts at: 513 1065 2340 3184 3768 513 Size: 552 1275 844 584 3493 Fragments arranged by size: 3493 1275 844 584 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3309 3901 3915 487 Size: 683 56 2083 592 14 3320 Fragments arranged by size: 3320 2083 683 592 56 14 BtsI GCAGTG_nn' Cuts at: 3097 3280 3564 3927 4264 5058 5078 3097 Size: 183 284 363 337 794 20 4767 Fragments arranged by size: 4767 794 363 337 284 183 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6748 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2140 2175 529 Size: 35 1576 35 5102 Fragments arranged by size: 5102 1576 35 35 DraI TTT'AAA Cuts at: 2502 4052 2502 Size: 1550 5198 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5755 809 Size: 507 4439 1802 Fragments arranged by size: 4439 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3252 3924 4195 Size: 54 1425 50 275 18 1422 672 271 Cuts at: 4195 5084 6713 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1422 889 672 275 271 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4195 1537 Size: 275 2383 4090 Fragments arranged by size: 4090 2383 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3266 3484 3961 4678 1353 Size: 1913 218 477 717 3423 Fragments arranged by size: 3423 1913 717 477 218 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5335 5709 5855 387 Size: 1064 138 3746 374 146 1280 Fragments arranged by size: 3746 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2716 3046 3371 3428 3440 4811 Size: 393 651 433 330 325 57 12 1371 Cuts at: 4811 5408 6195 6463 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1371 787 651 597 433 393 330 325 268 57 12 EcoNI CCTnn'n_nnAGG Cuts at: 3635 3635 Size: 6748 EcoRI G'AATT_C Cuts at: 4057 4435 4057 Size: 378 6370 FspI TGC'GCA Cuts at: 3080 5254 3080 Size: 2174 4574 HaeII r_GCGC'y Cuts at: 1678 2802 3938 5895 1678 Size: 1124 1136 1957 2531 Fragments arranged by size: 2531 1957 1136 1124 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3193 3225 3483 3515 5389 Size: 32 135 32 1621 32 258 32 1874 Cuts at: 5389 5421 5463 5495 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1874 1621 258 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4092 4338 669 Size: 988 281 2154 246 3079 Fragments arranged by size: 3079 2154 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6748 HpaI GTT'AAC Cuts at: 4338 4338 Size: 6748 KpnI G_GTAC'C Cuts at: 4073 4073 Size: 6748 MfeI C'AATT_G Cuts at: 4347 4347 Size: 6748 MluI A'CGCG_T Cuts at: 6624 6624 Size: 6748 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3000 5862 6046 1305 Size: 1695 2862 184 2007 Fragments arranged by size: 2862 2007 1695 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3254 3926 10 Size: 54 1425 343 1422 672 2832 Fragments arranged by size: 2832 1425 1422 672 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2066 2532 2600 2840 3920 4706 5065 Size: 648 900 466 68 240 1080 786 359 Cuts at: 5065 5224 518 Size: 159 2042 Fragments arranged by size: 2042 1080 900 786 648 466 359 240 159 68 MspA1I CmG'CkG Cuts at: 1606 2170 2639 2889 2937 3554 4164 4841 5989 Size: 564 469 250 48 617 610 677 1148 Cuts at: 5989 6234 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 617 610 564 469 250 245 48 NaeI GCC'GGC Cuts at: 3245 3620 3705 3245 Size: 375 85 6288 Fragments arranged by size: 6288 375 85 NcoI C'CATG_G Cuts at: 513 1065 3184 513 Size: 552 2119 4077 Fragments arranged by size: 4077 2119 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6748 NgoMIV G'CCGG_C Cuts at: 3243 3618 3703 3243 Size: 375 85 6288 Fragments arranged by size: 6288 375 85 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6748 NotI GC'GGCC_GC Cuts at: 4195 4195 Size: 6748 NspI r_CATG'y Cuts at: 1132 1229 2587 2839 4106 5651 1132 Size: 97 1358 252 1267 1545 2229 Fragments arranged by size: 2229 1545 1358 1267 252 97 PasI CC'CwG_GG Cuts at: 1348 3608 1348 Size: 2260 4488 PciI A'CATG_T Cuts at: 5647 5647 Size: 6748 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6748 PmeI GTTT'AAAC Cuts at: 4052 4052 Size: 6748 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4817 4849 6330 6362 4817 Size: 32 1481 32 5203 Fragments arranged by size: 5203 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6748 PsiI TTA'TAA Cuts at: 4318 4318 Size: 6748 PstI C_TGCA'G Cuts at: 830 3134 3559 3627 4100 830 Size: 2304 425 68 473 3478 Fragments arranged by size: 3478 2304 473 425 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6748 PvuII CAG'CTG Cuts at: 2170 2170 Size: 6748 SalI G'TCGA_C Cuts at: 1936 4090 1936 Size: 2154 4594 SbfI CC_TGCA'GG Cuts at: 4100 4100 Size: 6748 ScaI AGT'ACT Cuts at: 4996 6708 4996 Size: 1712 5036 SfcI C'TryA_G Cuts at: 826 1045 2794 3130 3555 3623 4096 5231 5912 Size: 219 1749 336 425 68 473 1135 681 Cuts at: 5912 6103 826 Size: 191 1471 Fragments arranged by size: 1749 1471 1135 681 473 425 336 219 191 68 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6748 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6748 SmaI CCC'GGG Cuts at: 1872 4075 1872 Size: 2203 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2909 4852 5753 6015 820 Size: 197 926 966 1943 901 262 1553 Fragments arranged by size: 1943 1553 966 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4539 493 Size: 4046 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6748 SphI G_CATG'C Cuts at: 1132 4106 1132 Size: 2974 3774 SspI AAT'ATT Cuts at: 5 52 4672 5 Size: 47 4620 2081 Fragments arranged by size: 4620 2081 47 StuI AGG'CCT Cuts at: 1517 3852 1517 Size: 2335 4413 StyI C'CwwG_G Cuts at: 513 1065 3184 3579 4187 513 Size: 552 2119 395 608 3074 Fragments arranged by size: 3074 2119 608 552 395 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2252 2963 3737 4775 4792 4945 5130 Size: 1255 672 711 774 1038 17 153 185 Cuts at: 5130 5575 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1038 774 711 672 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3351 4994 6706 Size: 274 80 33 51 1443 1374 1643 1712 Cuts at: 6706 96 Size: 138 Fragments arranged by size: 1712 1643 1443 1374 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2741 2773 6708 6740 2741 Size: 32 3935 32 2749 Fragments arranged by size: 3935 2749 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6748 XbaI T'CTAG_A Cuts at: 3211 4084 3211 Size: 873 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3794 3794 Size: 6748 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6748 XmaI C'CCGG_G Cuts at: 1870 4073 1870 Size: 2203 4545 XmnI GAAnn'nnTTC Cuts at: 2861 2945 3179 3275 4877 2861 Size: 84 234 96 1602 4732 Fragments arranged by size: 4732 1602 234 96 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4556 276 Size: 53 83 186 936 3022 2468 Fragments arranged by size: 3022 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI